##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2307555 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44036696850129 33.0 33.0 33.0 27.0 33.0 2 30.088198981172713 33.0 33.0 33.0 27.0 33.0 3 30.913024824977086 33.0 33.0 33.0 27.0 33.0 4 35.2439291804529 37.0 37.0 37.0 33.0 37.0 5 35.52233771242722 37.0 37.0 37.0 33.0 37.0 6 35.259718186565436 37.0 37.0 37.0 33.0 37.0 7 35.18114194461237 37.0 37.0 37.0 33.0 37.0 8 35.16788505582749 37.0 37.0 37.0 33.0 37.0 9 35.157937297269186 37.0 37.0 37.0 33.0 37.0 10-11 35.1175061916184 37.0 37.0 37.0 33.0 37.0 12-13 35.026918968345285 37.0 37.0 37.0 33.0 37.0 14-15 36.47160132694562 38.5 37.0 40.0 33.0 40.0 16-17 36.46264856092271 37.0 37.0 40.0 33.0 40.0 18-19 36.49037401058696 38.5 37.0 40.0 33.0 40.0 20-21 36.48900892936463 40.0 37.0 40.0 33.0 40.0 22-23 36.44936025360175 40.0 37.0 40.0 33.0 40.0 24-25 36.404589706420865 38.5 37.0 40.0 33.0 40.0 26-27 36.37537285135132 37.0 37.0 40.0 33.0 40.0 28-29 36.31498664170518 37.0 37.0 40.0 33.0 40.0 30-31 36.21240403804026 37.0 37.0 40.0 27.0 40.0 32-33 36.16021914970607 37.0 37.0 40.0 27.0 40.0 34-35 36.049938788024555 37.0 37.0 40.0 27.0 40.0 36-37 36.03000253515084 37.0 37.0 40.0 27.0 40.0 38-39 35.95649854499676 37.0 37.0 40.0 27.0 40.0 40-41 35.87863387871579 37.0 37.0 40.0 27.0 40.0 42-43 35.769789669151976 37.0 37.0 40.0 27.0 40.0 44-45 35.64590378127499 37.0 37.0 40.0 27.0 40.0 46-47 35.52220597125529 37.0 37.0 40.0 27.0 40.0 48-49 35.393015551091956 37.0 35.0 40.0 27.0 40.0 50-51 35.28278935930021 37.0 33.0 40.0 27.0 40.0 52-53 35.17458283767884 37.0 33.0 40.0 27.0 40.0 54-55 35.034636227522206 37.0 33.0 40.0 27.0 40.0 56-57 34.89935277815697 37.0 33.0 40.0 27.0 40.0 58-59 34.75743568408987 37.0 33.0 40.0 27.0 40.0 60-61 34.58587205938753 37.0 33.0 40.0 27.0 40.0 62-63 34.39585773686868 37.0 33.0 40.0 27.0 40.0 64-65 34.20536715267892 37.0 33.0 40.0 27.0 40.0 66-67 33.97032660109943 37.0 33.0 37.0 22.0 40.0 68-69 33.74936827074544 37.0 33.0 37.0 22.0 40.0 70-71 33.52085237404959 37.0 33.0 37.0 22.0 40.0 72-73 33.31199343027577 37.0 33.0 37.0 22.0 40.0 74-75 33.07733726823413 37.0 33.0 37.0 22.0 38.5 76-77 32.8502161378602 37.0 33.0 37.0 22.0 37.0 78-79 32.62492464968332 37.0 33.0 37.0 22.0 37.0 80-81 32.4257788005053 37.0 33.0 37.0 22.0 37.0 82-83 32.23029916946725 33.0 33.0 37.0 22.0 37.0 84-85 32.06562075443489 33.0 33.0 37.0 22.0 37.0 86-87 32.10051483063242 33.0 33.0 37.0 22.0 37.0 88-89 32.12933386203146 33.0 33.0 37.0 22.0 37.0 90-91 32.16500863468043 33.0 33.0 37.0 22.0 37.0 92-93 32.14316560168663 33.0 33.0 37.0 22.0 37.0 94-95 32.17989798726357 33.0 33.0 37.0 22.0 37.0 96-97 32.1542827798254 33.0 33.0 37.0 22.0 37.0 98-99 32.088732229567654 33.0 33.0 37.0 22.0 37.0 100 31.54811694629164 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6245105178742705 1101 2 4.136177029629902 1101 3 2.3164266442046824 1101 4 1.107635772568159 1101 5 0.4378410537547097 1101 6 0.4682481372441458 1101 7 0.5169574074586123 1101 8 0.40294282717677277 1101 9 0.518313463932202 1101 10-11 0.6332069362325683 1101 12-13 0.6245339302713333 1101 14-15 0.9189295575026861 1101 16-17 0.9053118687969501 1101 18-19 0.957371840853142 1101 20-21 0.8629181523311047 1101 22-23 0.8218224531130858 1101 24-25 0.8541055358627787 1101 26-27 0.8351373733664076 1101 28-29 0.8019246575703747 1101 30-31 0.7245128476350402 1101 32-33 0.9052854861673936 1101 34-35 1.0472100474609363 1101 36-37 0.9588311710980832 1101 38-39 0.9869398855454818 1101 40-41 0.9662304596517473 1101 42-43 0.9774645900049563 1101 44-45 0.977368497356288 1101 46-47 1.0279884929640062 1101 48-49 0.997322086936812 1101 50-51 1.0687866475078636 1101 52-53 0.8719968345130127 1101 54-55 1.0136519824533892 1101 56-57 0.992699159561468 1101 58-59 0.9596017634357565 1101 60-61 0.9417881921133926 1101 62-63 0.9331069391895426 1101 64-65 0.9558503401232485 1101 66-67 0.9459311747680204 1101 68-69 0.8478871838131568 1101 70-71 0.8341867123960611 1101 72-73 0.7785624197928911 1101 74-75 0.7303825743491359 1101 76-77 0.7801378390774047 1101 78-79 0.8946425102591746 1101 80-81 0.8833340811312027 1101 82-83 0.8919629721685496 1101 84-85 0.850274631599838 1101 86-87 0.8142974481872862 1101 88-89 0.9137175191672036 1101 90-91 0.8932898985395887 1101 92-93 0.950793199160163 1101 94-95 0.8593629908566953 1101 96-97 0.8083825195898307 1101 98-99 0.8413626527786917 1101 100 0.9810353312402853 1102 1 0.5458291764471923 1102 2 4.0394311568965655 1102 3 2.3323358307512834 1102 4 1.1170711220855623 1102 5 0.598153292644021 1102 6 0.562090183493261 1102 7 0.5629423528867221 1102 8 0.5200880392306786 1102 9 0.5866931176515848 1102 10-11 0.6074655080389135 1102 12-13 0.6928442132712576 1102 14-15 0.9311838155139398 1102 16-17 0.9832817624481791 1102 18-19 0.8845537615470747 1102 20-21 0.9028143905048864 1102 22-23 0.929290100426293 1102 24-25 0.9063666680581335 1102 26-27 0.910312698446063 1102 28-29 0.8977382569332377 1102 30-31 0.8603966554454701 1102 32-33 1.0239881232795724 1102 34-35 1.0389355220195498 1102 36-37 0.8583410263985982 1102 38-39 1.0119003205554051 1102 40-41 0.8959513409137614 1102 42-43 0.905911487903218 1102 44-45 0.9888475851317153 1102 46-47 0.908281649664815 1102 48-49 0.8414023499849819 1102 50-51 0.819756420662884 1102 52-53 0.9026187859086363 1102 54-55 0.830945539928301 1102 56-57 0.7860448538486224 1102 58-59 0.8754789548245654 1102 60-61 0.8402427351540638 1102 62-63 0.9704198847518057 1102 64-65 0.9338258936798525 1102 66-67 0.8755650223685905 1102 68-69 0.8025179374874014 1102 70-71 0.8387340465185247 1102 72-73 0.7559080662542357 1102 74-75 0.7812791763407034 1102 76-77 0.8485867664671147 1102 78-79 0.8330027634689046 1102 80-81 0.7642687908230883 1102 82-83 0.8709619670500999 1102 84-85 0.8052467847101283 1102 86-87 0.9362587039999433 1102 88-89 0.8602439715773365 1102 90-91 0.8409114533536375 1102 92-93 0.977669306393711 1102 94-95 0.8956540672899536 1102 96-97 0.8802327667234984 1102 98-99 0.8484896692849659 1102 100 0.8363122108606973 1103 1 0.5518217756948118 1103 2 4.036109307162889 1103 3 2.347100177124922 1103 4 1.0788672466941378 1103 5 0.5052269407333796 1103 6 0.4671003180303046 1103 7 0.4317348108851178 1103 8 0.5195537788783184 1103 9 0.44978898125204836 1103 10-11 0.49715967948883133 1103 12-13 0.5218223622437463 1103 14-15 0.8125935985688884 1103 16-17 0.7484561922996065 1103 18-19 0.7743476137346192 1103 20-21 0.7322312609510107 1103 22-23 0.6758566571798568 1103 24-25 0.6871041130187479 1103 26-27 0.684079670994933 1103 28-29 0.7623059005105759 1103 30-31 0.6507370988910282 1103 32-33 0.796167856603816 1103 34-35 0.8788924372198395 1103 36-37 0.7520234529567418 1103 38-39 0.6070611067291409 1103 40-41 0.6773213463447334 1103 42-43 0.6990270162608283 1103 44-45 0.7793934647789271 1103 46-47 0.7158209058090463 1103 48-49 0.5971934971047617 1103 50-51 0.6485774551209431 1103 52-53 0.649355237769818 1103 54-55 0.6448555273149523 1103 56-57 0.5636398507355267 1103 58-59 0.5702796517297983 1103 60-61 0.5728287035774784 1103 62-63 0.6200145190485529 1103 64-65 0.5865980832352946 1103 66-67 0.5920554509665692 1103 68-69 0.6017959207539718 1103 70-71 0.7258250004777764 1103 72-73 0.5825619223485461 1103 74-75 0.5736595444659685 1103 76-77 0.5358327521774626 1103 78-79 0.5818442263639909 1103 80-81 0.622734174507535 1103 82-83 0.6438885367041749 1103 84-85 0.5432885674671937 1103 86-87 0.5590249797697986 1103 88-89 0.6248043412615125 1103 90-91 0.6861827679906973 1103 92-93 0.655267837666873 1103 94-95 0.6239985749641477 1103 96-97 0.5817748320736733 1103 98-99 0.5685382702517998 1103 100 0.6990613417014657 1104 1 0.41749308753779957 1104 2 3.5942093708264267 1104 3 2.1444720601258815 1104 4 0.9513212112838687 1104 5 0.44280290658990396 1104 6 0.3895175274943554 1104 7 0.4007657630898507 1104 8 0.41526145392187175 1104 9 0.36046965314125146 1104 10-11 0.4073621055700585 1104 12-13 0.44020662991628257 1104 14-15 0.5203145961511026 1104 16-17 0.5347307643441042 1104 18-19 0.5658115090912617 1104 20-21 0.5691432457456145 1104 22-23 0.49177801562468204 1104 24-25 0.5993553195245767 1104 26-27 0.5301954987934039 1104 28-29 0.5414869519355321 1104 30-31 0.5319594161433869 1104 32-33 0.6166676129462374 1104 34-35 0.7009720809372411 1104 36-37 0.6285611314547381 1104 38-39 0.6386911232693535 1104 40-41 0.6222457056079023 1104 42-43 0.6625084222484432 1104 44-45 0.7625628319769575 1104 46-47 0.6262475551619033 1104 48-49 0.6234122748846787 1104 50-51 0.5260159135661127 1104 52-53 0.5133823265970534 1104 54-55 0.6155443470357227 1104 56-57 0.6224893901992274 1104 58-59 0.5303913924943373 1104 60-61 0.6335245828088318 1104 62-63 0.6291822466392176 1104 64-65 0.6463137043175635 1104 66-67 0.581079078058572 1104 68-69 0.6169943555152031 1104 70-71 0.5306569431517616 1104 72-73 0.4886110354236308 1104 74-75 0.4379372143383833 1104 76-77 0.5187832810253283 1104 78-79 0.5236010371559914 1104 80-81 0.5907758801980165 1104 82-83 0.5727505659466416 1104 84-85 0.5703003053003854 1104 86-87 0.6563709989524291 1104 88-89 0.571894613113404 1104 90-91 0.5891862423582275 1104 92-93 0.5968319249534524 1104 94-95 0.5437959998865978 1104 96-97 0.5664910483314749 1104 98-99 0.5284709320112029 1104 100 0.49048057864918704 1105 1 0.44035207684956035 1105 2 3.634796474636836 1105 3 2.108521877713901 1105 4 0.9327873123337653 1105 5 0.2659106836355676 1105 6 0.24660428243741706 1105 7 0.2770597599202276 1105 8 0.2234115792130993 1105 9 0.29072767303170366 1105 10-11 0.2836642397832776 1105 12-13 0.37749398215548524 1105 14-15 0.42733043553621286 1105 16-17 0.4357943427155888 1105 18-19 0.4886226000136915 1105 20-21 0.3877511724457037 1105 22-23 0.37529515319769047 1105 24-25 0.4313148559703066 1105 26-27 0.38778089787308545 1105 28-29 0.41597479648027047 1105 30-31 0.25569828699769914 1105 32-33 0.36073925422005715 1105 34-35 0.6252822858026832 1105 36-37 0.44627155989223155 1105 38-39 0.4595584483610935 1105 40-41 0.35619994912456576 1105 42-43 0.4813842503546084 1105 44-45 0.43985615244601917 1105 46-47 0.3827875432401342 1105 48-49 0.364834086545919 1105 50-51 0.34540211261376186 1105 52-53 0.3956733910328083 1105 54-55 0.33996051734923327 1105 56-57 0.27317530427123415 1105 58-59 0.2507650578513818 1105 60-61 0.3735591236536422 1105 62-63 0.5038869249042293 1105 64-65 0.336959980565517 1105 66-67 0.38442384424485 1105 68-69 0.32293175305861865 1105 70-71 0.2798838917816653 1105 72-73 0.2688400462580631 1105 74-75 0.29851935250975714 1105 76-77 0.34944960582674867 1105 78-79 0.30533432172757813 1105 80-81 0.4183710261900089 1105 82-83 0.42673999640408056 1105 84-85 0.3560253330592573 1105 86-87 0.3259331327039199 1105 88-89 0.33191301624318825 1105 90-91 0.3891008875344859 1105 92-93 0.37226848636548837 1105 94-95 0.4108625920673177 1105 96-97 0.3459466203013797 1105 98-99 0.39278431514667744 1105 100 0.3547206029724137 1106 1 0.34803065092690844 1106 2 3.7783665371208173 1106 3 2.0304737737810683 1106 4 0.8485366580696052 1106 5 0.1781556419762751 1106 6 0.12485653630039906 1106 7 0.03566874827590283 1106 8 0.038976232600433036 1106 9 0.0748624622754761 1106 10-11 0.10725137782208094 1106 12-13 0.08243607701123778 1106 14-15 0.19795733613065636 1106 16-17 0.14561905711232015 1106 18-19 0.12411929305768865 1106 20-21 0.01245985405692096 1106 22-23 0.0061969855339398805 1106 24-25 0.1000740785903389 1106 26-27 0.17101744963426313 1106 28-29 0.0872645755546202 1106 30-31 -0.03565282192978003 1106 32-33 0.10938811039108742 1106 34-35 0.2683668209437613 1106 36-37 0.15553241689359965 1106 38-39 0.0814370750921043 1106 40-41 0.0762035844322213 1106 42-43 0.14147556852128673 1106 44-45 0.17612189986027715 1106 46-47 0.015288181183876759 1106 48-49 -0.1283352552539938 1106 50-51 -0.18932647791884705 1106 52-53 -0.09468659881720498 1106 54-55 -0.05648562635719401 1106 56-57 -0.031289127975483666 1106 58-59 0.038602697063709 1106 60-61 0.05460718134199283 1106 62-63 0.038925365327898476 1106 64-65 -0.024648485616246774 1106 66-67 -0.08491031699417562 1106 68-69 -0.09875782656956744 1106 70-71 -0.06767485992206446 1106 72-73 -0.01369294298886814 1106 74-75 -0.07536111273664403 1106 76-77 -0.09695326399600646 1106 78-79 -0.16022163404937828 1106 80-81 -0.1809272079293578 1106 82-83 0.007344614775703917 1106 84-85 0.028089786437252684 1106 86-87 0.10211147250500474 1106 88-89 0.001686325501896846 1106 90-91 0.040049700451461945 1106 92-93 0.11679767753690484 1106 94-95 0.07579486611922448 1106 96-97 -0.0018260616823333464 1106 98-99 0.10522358754258931 1106 100 0.10653351733343541 1107 1 0.40670204456471737 1107 2 3.4272593651523238 1107 3 2.127360190027421 1107 4 0.8747152269980774 1107 5 0.2671142964031219 1107 6 0.08256612261527607 1107 7 0.048735390579999205 1107 8 0.08159182329978165 1107 9 0.09411146236209333 1107 10-11 0.15828200149198324 1107 12-13 0.12091230511607165 1107 14-15 0.09095897071664893 1107 16-17 0.013775676790075408 1107 18-19 -0.061087901493635854 1107 20-21 0.10139408659051696 1107 22-23 0.04954533190480248 1107 24-25 0.10425107265275102 1107 26-27 0.04517386602694984 1107 28-29 0.010985768102365512 1107 30-31 -0.048577520630843196 1107 32-33 0.07693507260419352 1107 34-35 0.1704524308523716 1107 36-37 -0.07054427220165849 1107 38-39 -0.11226820664162318 1107 40-41 -0.11541804509932518 1107 42-43 -0.04849785017595565 1107 44-45 -0.09282422920043132 1107 46-47 -0.11865573903029514 1107 48-49 -0.17916281137589607 1107 50-51 -0.22125008465478402 1107 52-53 -0.199483078426006 1107 54-55 -0.16834246541671405 1107 56-57 -0.16308141018262745 1107 58-59 -0.12845990089576276 1107 60-61 -0.13260873864753364 1107 62-63 -0.06511886064441796 1107 64-65 -0.2119209220194591 1107 66-67 -0.2754601714713303 1107 68-69 -0.20730786218400254 1107 70-71 -0.1630820213308013 1107 72-73 -0.20120789769070768 1107 74-75 -0.06329841788058843 1107 76-77 -0.16525813544192403 1107 78-79 -0.17362369672050448 1107 80-81 -0.16066249438136282 1107 82-83 -0.13283244493909052 1107 84-85 0.014630515234006936 1107 86-87 -0.09210291177259577 1107 88-89 -0.012957793132741813 1107 90-91 -0.03708964750969557 1107 92-93 0.006753081380100667 1107 94-95 -0.16096745922468614 1107 96-97 -0.1454236497880217 1107 98-99 -0.109517625420672 1107 100 -0.017101892078347447 1108 1 0.2568874823684517 1108 2 3.497037976571317 1108 3 1.9709297848588463 1108 4 0.8342156580284765 1108 5 0.19928574229696494 1108 6 0.05547475680151592 1108 7 0.018755055355185846 1108 8 -7.668366219633072E-4 1108 9 -0.0037590816835333385 1108 10-11 0.01673840742472521 1108 12-13 0.007065039080323743 1108 14-15 -0.054657051456949546 1108 16-17 -0.12160728108893437 1108 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2211 70-71 -0.19981151193826463 2211 72-73 -0.04373398116818805 2211 74-75 -0.04981109394859118 2211 76-77 -0.12500381700766638 2211 78-79 0.09435358769938063 2211 80-81 -0.08727566391076635 2211 82-83 -0.712255508126372 2211 84-85 -0.1546212598088772 2211 86-87 -0.11622258470302427 2211 88-89 -0.06950617191483488 2211 90-91 -0.15816894811329618 2211 92-93 -0.07117831433826538 2211 94-95 0.016552770415501072 2211 96-97 -0.21251700995273737 2211 98-99 -0.13616526582218924 2211 100 -0.13567693040519657 2212 1 0.7746871027936777 2212 2 4.09746371584448 2212 3 2.4273235680395757 2212 4 1.1426490144440606 2212 5 0.6742043132492341 2212 6 0.6417350476378658 2212 7 0.557856707018388 2212 8 0.3682833929181868 2212 9 0.4589909760220934 2212 10-11 0.35435734512829953 2212 12-13 0.34530254901850554 2212 14-15 0.5577123094211984 2212 16-17 0.5096398336298407 2212 18-19 0.6005932918948673 2212 20-21 0.5533155202192717 2212 22-23 0.5144333906953023 2212 24-25 0.5086188550693151 2212 26-27 0.44775693690269947 2212 28-29 0.4343347268171769 2212 30-31 0.39519185850438276 2212 32-33 0.4628111926266527 2212 34-35 0.45429480981669457 2212 36-37 0.5185741144777012 2212 38-39 0.44392579223529793 2212 40-41 0.5168727823134489 2212 42-43 0.4756848691287132 2212 44-45 0.5419837495099884 2212 46-47 0.7001911566458645 2212 48-49 0.721608691834632 2212 50-51 0.548822426881145 2212 52-53 0.6178525498852281 2212 54-55 0.5941834751580117 2212 56-57 0.5385751244007366 2212 58-59 0.6409936990456373 2212 60-61 0.6442083223158761 2212 62-63 0.5926095995230725 2212 64-65 0.5341763948095632 2212 66-67 0.631322220676914 2212 68-69 0.5844497490857989 2212 70-71 0.8771472323130851 2212 72-73 0.8464788134106342 2212 74-75 0.771509560829827 2212 76-77 0.559312776933865 2212 78-79 0.5441135527240633 2212 80-81 0.5011761735548674 2212 82-83 0.7342810099792487 2212 84-85 0.836829543530591 2212 86-87 0.7393041050002687 2212 88-89 0.7986074306276016 2212 90-91 0.6650173375241231 2212 92-93 0.651605250622513 2212 94-95 0.7573166785716481 2212 96-97 0.5389643967951478 2212 98-99 0.6962234815399526 2212 100 0.7914432384180046 2213 1 -0.527233421513607 2213 2 -25.911479373586413 2213 3 -14.757482305529361 2213 4 -6.220176603608039 2213 5 -1.962820740674772 2213 6 -1.0589493724716448 2213 7 -0.8342828317239395 2213 8 -1.0539125586331721 2213 9 -1.076984633734007 2213 10-11 -0.9570496566968245 2213 12-13 -0.8653972186934524 2213 14-15 -1.0903049462615613 2213 16-17 -1.1647939642795677 2213 18-19 -1.10273133063707 2213 20-21 -1.3140397145575662 2213 22-23 -1.25906710706478 2213 24-25 -1.2075645695200308 2213 26-27 -1.1076769439667231 2213 28-29 -1.1762841548818415 2213 30-31 -1.1920426694531443 2213 32-33 -1.1995996253590775 2213 34-35 -1.1629919829491868 2213 36-37 -1.1202138397283719 2213 38-39 -1.2526251452128463 2213 40-41 -1.2256691124932502 2213 42-43 -1.21354069914738 2213 44-45 -1.0988636870256059 2213 46-47 -1.2196549525295026 2213 48-49 -1.3132428097416096 2213 50-51 -1.1456088822278616 2213 52-53 -1.338469003126221 2213 54-55 -1.2112006297034483 2213 56-57 -1.237192255722043 2213 58-59 -1.3650582339226673 2213 60-61 -1.2834235841052362 2213 62-63 -1.1569527864069116 2213 64-65 -1.1089151100850785 2213 66-67 -1.2361844161241464 2213 68-69 -1.1755697465173185 2213 70-71 -1.0686176590092913 2213 72-73 -1.1605105677076644 2213 74-75 -1.2985045423724273 2213 76-77 -1.0868378784502397 2213 78-79 -1.168054607222846 2213 80-81 -1.2197110837870895 2213 82-83 -1.073009101187175 2213 84-85 -1.1704522716344954 2213 86-87 -1.071569878739563 2213 88-89 -0.9222487167093796 2213 90-91 -1.088981335115669 2213 92-93 -1.0657022821466917 2213 94-95 -1.1441143816573245 2213 96-97 -1.2490686212901352 2213 98-99 -1.1165253984998742 2213 100 -1.2312338812103363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 21.0 5 43.0 6 38.0 7 31.0 8 39.0 9 69.0 10 298.0 11 1416.0 12 4055.0 13 7307.0 14 9692.0 15 11367.0 16 12185.0 17 13080.0 18 13698.0 19 14735.0 20 16311.0 21 18235.0 22 20646.0 23 22528.0 24 23514.0 25 24163.0 26 26307.0 27 29627.0 28 34635.0 29 41552.0 30 50324.0 31 62038.0 32 78520.0 33 104557.0 34 144486.0 35 205550.0 36 315569.0 37 527649.0 38 471517.0 39 1741.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.43272177929914 20.13032999467328 10.429062933887545 31.00788529214003 2 13.258562142766825 22.711829308742924 35.69564603884645 28.333962509643808 3 15.641230653223866 26.512434156498976 29.037921089638168 28.80841410063899 4 11.150028884962442 18.852497736658417 36.23236077903155 33.76511259934759 5 11.95995810194701 38.28284832097416 34.68340100022269 15.073792576856142 6 30.000845537621 38.692972281542346 17.799781027436612 13.506401153400036 7 26.13287818928507 33.16596356044148 22.70499148339012 17.996166766883327 8 24.81678152866463 35.907655462563774 20.81159799803688 18.46396501073471 9 25.30871717678397 16.77649106180153 21.457659259824474 36.45713250159003 10-11 23.37835280458162 27.243048566011012 28.88359327393795 20.495005355469416 12-13 24.39017516964628 24.815743244063466 28.78348487187002 22.010596714420238 14-15 21.953992166047364 26.337823328304786 26.211202825408296 25.49698168023955 16-17 21.281390723811487 29.30148425519001 27.18891062496952 22.22821439602898 18-19 21.48013043718565 28.386148689288348 29.001661350993725 21.132059522532277 20-21 22.39669022053276 27.739342513149758 28.52118666751337 21.342780598804108 22-23 22.03203623436079 27.53307731336381 28.290690670904816 22.144195781370595 24-25 21.951715865019413 27.695250149269256 28.211163715054465 22.14187027065686 26-27 21.802272664230287 27.434025297670644 28.505799983356795 22.257902054742274 28-29 21.684753928358187 27.44041497939515 28.733248739490335 22.141582352756327 30-31 22.099957736863207 27.781445399278276 28.307935716685268 21.810661147173246 32-33 21.59629021472846 27.64529782853359 28.585181747149214 22.17323020958874 34-35 21.589949509625153 27.52788281708599 28.616911296118975 22.26525637716988 36-37 22.120611826378187 27.597453814857253 28.251421914735385 22.03051244402917 38-39 22.06334478054125 27.683903642361717 28.247394041095536 22.005357536001497 40-41 21.869203392239868 27.61646956033326 28.28683912170666 22.22748792572021 42-43 21.990243893982576 27.420396030034986 28.27012768402889 22.31923239195354 44-45 22.183216478626477 27.482840181550067 28.132134123416343 22.201809216407113 46-47 22.033209948247613 27.687634907287812 28.137174163683177 22.1419809807814 48-49 22.14577876957035 27.673331790159576 27.94130651332028 22.239582926949797 50-51 21.97764901092842 27.67118246459056 27.9343583821844 22.41681014229662 52-53 21.99230650522523 27.607434964220133 27.659474858727183 22.74078367182745 54-55 22.141572834993813 27.880750838329792 27.5111531672741 22.4665231594023 56-57 22.161031494779763 27.479329708700163 27.692837477504966 22.666801319015107 58-59 21.9018662170611 27.280662879259886 28.06301557185472 22.754455331824293 60-61 22.135840486463408 27.627407341592754 27.744450680784393 22.49230149115945 62-63 21.867018564122283 27.677639333859677 27.790182659138928 22.665159442879116 64-65 21.816129639085215 27.434958965481073 27.913572209396982 22.835339186036734 66-67 21.989488287075396 27.440962911221302 27.836295382990926 22.733253418712373 68-69 22.257437991720018 27.4014641793636 27.695756558777525 22.64534127013886 70-71 21.990111387769538 27.48589446598373 27.697412453790722 22.82658169245601 72-73 22.106736495603975 27.484589099732943 27.74551565667709 22.663158747985996 74-75 22.270939502024632 27.385285796923792 27.518252905390924 22.825521795660656 76-77 22.01144676111794 27.484428066128462 27.747072047324895 22.7570531254287 78-79 22.13096031671043 27.50691525464867 27.7410283726982 22.6210960559427 80-81 21.958886722538146 27.451918607334807 27.767054377715205 22.82214029241184 82-83 21.824906933742287 27.456815934564222 27.673340306666294 23.0449368250272 84-85 21.938864734807254 27.47327117247979 27.474203000181813 23.11366109253114 86-87 22.006426837997004 27.433613935953954 27.55511666927788 23.00484255677116 88-89 21.847312400685713 27.23991168355339 27.73495271377614 23.177823201984758 90-91 21.819819648024573 27.326722074459443 27.745357607361086 23.1081006701549 92-93 21.627536640452377 27.509944391151038 27.696340104352995 23.16617886404359 94-95 21.48429916100402 27.293436243239494 27.87076601719595 23.351498578560534 96-97 21.720351682792106 27.26677050045633 27.71241940530454 23.300458411447025 98-99 21.68203404535437 27.309044980595132 27.61627699114143 23.392643982909068 100 21.591804844019855 27.215584435867214 27.680733201479 23.511877518633934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 9.5 2 9.0 3 10.0 4 17.0 5 36.0 6 89.5 7 180.5 8 315.0 9 383.5 10 319.5 11 274.5 12 307.0 13 375.0 14 452.0 15 538.0 16 666.0 17 775.5 18 903.5 19 1065.5 20 1270.5 21 1674.5 22 2265.5 23 3187.5 24 4585.5 25 6633.0 26 9699.5 27 13991.0 28 19664.5 29 26059.0 30 32659.5 31 39523.0 32 46510.0 33 53388.5 34 60058.0 35 65909.0 36 71198.5 37 76634.0 38 80747.5 39 83637.5 40 85779.0 41 86542.0 42 86122.0 43 85059.0 44 83674.5 45 81919.0 46 80144.5 47 78519.0 48 76679.0 49 76197.0 50 81713.5 51 79319.5 52 67961.0 53 62528.5 54 59326.0 55 55970.5 56 51993.0 57 47480.0 58 43317.0 59 39778.5 60 36001.0 61 30988.5 62 26397.5 63 21977.0 64 17586.0 65 14114.5 66 11069.5 67 8608.0 68 6642.5 69 5045.0 70 3778.5 71 2778.5 72 2077.0 73 1521.5 74 1122.0 75 780.5 76 453.0 77 265.5 78 141.0 79 71.5 80 44.0 81 19.0 82 7.0 83 7.0 84 6.0 85 2.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7460277219827913 2 5.408625146529552 3 0.0 4 0.005460324889330915 5 0.16762330692009508 6 0.05763676272071522 7 0.03839561787259675 8 0.04415929414466827 9 0.04307589634916611 10-11 0.04483100077787962 12-13 0.04294588861370585 14-15 0.04578439083792152 16-17 0.036510505708422986 18-19 0.03022679849451042 20-21 0.02485314542881968 22-23 0.02485314542881968 24-25 0.0209095774531918 26-27 0.013304124928766595 28-29 0.0196095000985892 30-31 0.025134828855650247 32-33 0.0015600928255231185 34-35 0.003705220460617407 36-37 0.004463598917468923 38-39 0.006543722684833081 40-41 0.007410440921234814 42-43 0.00777879617170555 44-45 0.008775522143567542 46-47 0.017984403405335952 48-49 0.026911601240273798 50-51 0.02353140011830704 52-53 0.02907839683127813 54-55 0.025438180238390853 56-57 0.01642431057981283 58-59 0.015059229357480103 60-61 0.012567414427825123 62-63 0.01921947689220842 64-65 0.020497886290900977 66-67 0.022079647072334136 68-69 0.02884004931626765 70-71 0.02855836588943709 72-73 0.02530817250293059 74-75 0.025719863665221412 76-77 0.019046133244928074 78-79 0.014604202283369195 80-81 0.012805761942835598 82-83 0.008385498937186762 84-85 0.011332340940952652 86-87 0.008645514408107282 88-89 0.00738877296532477 90-91 0.00771379230397542 92-93 0.004268587314278533 94-95 0.0015167569137030318 96-97 9.100541482218192E-4 98-99 6.717066332113427E-4 100 0.10773307678473537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2307555.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.39509048611895 #Duplication Level Percentage of deduplicated Percentage of total 1 85.87558279899281 67.32224084075243 2 9.871619630043867 15.47773028283674 3 2.3407299199555887 5.50505201635453 4 0.8013899999546418 2.5130016624446005 5 0.37287517076996135 1.461579137626909 6 0.19976401631401908 0.9396310880884842 7 0.13197917304585857 0.7242563449249263 8 0.08364279158786006 0.5245747372033503 9 0.06149747502060836 0.43389901070175885 >10 0.24926425618331596 3.186964419670553 >50 0.00787491915870534 0.4031917930023164 >100 0.002891931103920246 0.4891162026737106 >500 3.3296920081943114E-4 0.17802348727768175 >1k 5.549486680236106E-4 0.8407389764419608 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4870 0.2110458905638219 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3506 0.15193570684122373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.333591182008663E-5 0.0 6 0.0 0.0 0.0 4.333591182008663E-5 0.0 7 0.0 0.0 0.0 1.7334364728034652E-4 0.0 8 0.0 0.0 0.0 1.7334364728034652E-4 0.0 9 0.0 0.0 0.0 1.7334364728034652E-4 0.0 10-11 0.0 0.0 0.0 1.7334364728034652E-4 0.0 12-13 0.0 0.0 0.0 2.3834751501047646E-4 0.0 14-15 0.0 6.500386773012994E-5 0.0 2.6001547092051975E-4 0.0 16-17 0.0 1.3000773546025988E-4 0.0 2.6001547092051975E-4 0.0 18-19 0.0 1.516756913703032E-4 0.0 2.6001547092051975E-4 0.0 20-21 0.0 1.7334364728034652E-4 0.0 2.6001547092051975E-4 0.0 22-23 0.0 1.7334364728034652E-4 0.0 3.033513827406064E-4 0.0 24-25 0.0 2.1667955910043314E-4 0.0 3.4668729456069304E-4 0.0 26-27 0.0 2.1667955910043314E-4 0.0 5.200309418410395E-4 0.0 28-29 0.0 2.1667955910043314E-4 0.0 0.0011484016632322958 0.0 30-31 0.0 2.1667955910043314E-4 0.0 0.00346687294560693 0.0 32-33 0.0 2.1667955910043314E-4 0.0 0.009425560820868841 0.0 34-35 0.0 2.1667955910043314E-4 0.0 0.019306148715848594 0.0 36-37 0.0 2.1667955910043314E-4 0.0 0.034170366470138304 0.0 38-39 0.0 2.3834751501047646E-4 0.0 0.0571817356466043 0.0 40-41 0.0 2.6001547092051975E-4 0.0 0.08680183137563352 0.0 42-43 0.0 2.6001547092051975E-4 0.0 0.1167036105314933 0.0 44-45 0.0 2.6001547092051975E-4 0.0 0.15360413944629706 0.0 46-47 0.0 2.816834268305631E-4 0.0 0.19798011315006575 0.0 48-49 0.0 3.033513827406064E-4 0.0 0.24512958521032002 0.0 50-51 0.0 3.033513827406064E-4 0.0 0.2990394595145078 0.0 52-53 0.0 3.033513827406064E-4 0.0 0.3547261062033191 0.0 54-55 0.0 3.250193386506497E-4 0.0 0.4218100977008132 0.0 56-57 0.0 3.4668729456069304E-4 0.0 0.497756283165515 0.0 58-59 0.0 3.4668729456069304E-4 0.0 0.5834530487897364 0.0 60-61 0.0 3.4668729456069304E-4 0.0 0.6741551122291776 0.0 62-63 0.0 3.4668729456069304E-4 0.0 0.7721809447662136 0.0 64-65 0.0 3.4668729456069304E-4 0.0 0.8843992884243279 0.0 66-67 0.0 3.683552504707364E-4 0.0 1.0136269774718263 0.0 68-69 0.0 3.900232063807797E-4 0.0 1.1524102350756538 0.0 70-71 0.0 3.900232063807797E-4 0.0 1.3182567696111254 0.0 72-73 0.0 4.11691162290823E-4 0.0 1.4880252041663145 0.0 74-75 0.0 4.3335911820086627E-4 0.0 1.670404389061149 0.0 76-77 0.0 4.3335911820086627E-4 0.0 1.8813202718895108 0.0 78-79 0.0 4.3335911820086627E-4 0.0 2.104110194556576 0.0 80-81 4.333591182008663E-5 4.3335911820086627E-4 0.0 2.3554368151571685 0.0 82-83 4.333591182008663E-5 4.3335911820086627E-4 0.0 2.627759685034593 0.0 84-85 4.333591182008663E-5 4.3335911820086627E-4 0.0 2.9136250273557946 0.0 86-87 8.667182364017326E-5 4.766950300209529E-4 0.0 3.225903607931339 0.0 88 8.667182364017326E-5 4.766950300209529E-4 0.0 3.490404345725237 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9640 0.0 21.062366 1 ACCGTAC 170 8.705972E-5 19.33151 8 GAGTACT 4425 0.0 18.88487 12-13 TATCAAC 10735 0.0 18.619968 2 ATCAACG 10650 0.0 18.503902 3 CAACGCA 10630 0.0 18.495275 5 TCAACGC 10690 0.0 18.479462 4 AACGCAG 10635 0.0 18.456112 6 ACGCAGA 11695 0.0 16.6596 7 CGCAGAG 11895 0.0 16.418957 8 GTACTTT 5195 0.0 16.22132 14-15 GGTATCA 7425 0.0 16.018635 1 AGTACTT 5045 0.0 15.540414 12-13 GCAGAGT 12840 0.0 14.6617775 9 ATAGTAC 710 0.0 14.573478 3 CTAGTAC 520 8.913048E-11 14.471566 3 CATGGGG 4590 0.0 14.346076 4 GTACATG 10940 0.0 13.965135 1 ACATGGG 10860 0.0 13.945207 3 TATACTG 1165 0.0 13.323115 5 >>END_MODULE