FastQCFastQC Report
Wed 25 May 2016
SRR1295081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272935
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27120.11931709441757024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT8000.018.0423151
ATAGGAC6400.016.8321063
GTACATG95150.016.7524871
GTATTAC6101.0913936E-1116.4605581
TATACGG1850.002114514116.3494635
TACATGG96150.016.31232
GTACAAG8200.016.0716271
GTCCTAA6803.6379788E-1215.688971
GTATAGG5851.8208084E-915.01851
GTATATA9350.014.76608851
CATGGGG29950.014.7651044
ACATGGG99150.014.7533893
GTCTAAG6454.6020432E-1014.594391
TAGACAG9850.014.3299865
GTACATA8800.014.2626991
TATATAG6951.8189894E-1213.7813825
GTCCTAG8205.456968E-1213.7756811
GTGTTAA7804.0017767E-1113.6775621
TATACTG8600.013.481975
TAGTCCG2250.00776809113.4428915
Produced by FastQC (version 0.11.5)