##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295081_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2272935 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.03140476960406 33.0 27.0 33.0 6.0 33.0 2 22.057909707052776 27.0 2.0 33.0 2.0 33.0 3 24.408966380472826 27.0 15.0 33.0 6.0 33.0 4 24.361941718526925 33.0 2.0 37.0 2.0 37.0 5 26.398420544362246 33.0 15.0 37.0 2.0 37.0 6 27.301147195146363 33.0 15.0 37.0 2.0 37.0 7 27.035963632923952 33.0 15.0 37.0 6.0 37.0 8 27.83707277154868 33.0 15.0 37.0 6.0 37.0 9 28.871650531141455 33.0 22.0 37.0 15.0 37.0 10-11 28.384838545756917 33.0 18.5 37.0 10.5 37.0 12-13 27.175365551588584 33.0 15.0 37.0 4.0 37.0 14-15 28.939216255634236 33.0 22.0 37.0 4.0 40.0 16-17 29.351283912650384 33.0 27.0 37.0 6.0 40.0 18-19 29.55347205265439 33.0 22.0 40.0 6.0 40.0 20-21 28.98355584299595 33.0 22.0 38.5 6.0 40.0 22-23 29.783699709846523 33.0 27.0 37.0 6.0 40.0 24-25 29.923962409835738 33.0 27.0 38.5 6.0 40.0 26-27 28.418632956947736 33.0 18.5 37.0 4.0 40.0 28-29 29.15736481685574 33.0 22.0 37.0 6.0 40.0 30-31 29.907347988393862 33.0 27.0 38.5 10.5 40.0 32-33 28.596810951479036 33.0 22.0 37.0 4.0 40.0 34-35 28.21420916128266 33.0 22.0 37.0 6.0 40.0 36-37 28.52234775741497 33.0 22.0 37.0 6.0 40.0 38-39 28.686683957086323 33.0 22.0 37.0 6.0 40.0 40-41 27.693117049101712 33.0 15.0 37.0 4.0 40.0 42-43 28.334943806136117 33.0 22.0 37.0 6.0 40.0 44-45 28.208934703368115 33.0 22.0 37.0 6.0 40.0 46-47 27.9452575634587 33.0 22.0 37.0 6.0 40.0 48-49 27.888982526997033 33.0 22.0 37.0 6.0 40.0 50-51 27.7932459573195 33.0 22.0 37.0 6.0 40.0 52-53 27.98890267429557 33.0 22.0 37.0 6.0 40.0 54-55 27.944051413700787 33.0 22.0 37.0 6.0 40.0 56-57 27.92154747056119 33.0 22.0 37.0 6.0 40.0 58-59 27.83997936588596 33.0 22.0 37.0 6.0 40.0 60-61 27.770898419884425 33.0 22.0 37.0 6.0 40.0 62-63 27.758251335827907 33.0 22.0 37.0 6.0 40.0 64-65 27.266672826103694 33.0 22.0 37.0 6.0 40.0 66-67 27.46116188980327 33.0 22.0 37.0 6.0 37.0 68-69 26.9956054616608 33.0 22.0 37.0 6.0 37.0 70-71 27.14276189156311 33.0 22.0 37.0 10.5 37.0 72-73 27.20855633795071 27.0 22.0 37.0 10.5 37.0 74-75 27.32654585370897 27.0 22.0 37.0 10.5 37.0 76-77 27.076286607404082 27.0 22.0 35.0 10.5 37.0 78-79 26.62692751882478 27.0 22.0 33.0 6.0 37.0 80-81 26.413060206297146 27.0 22.0 33.0 6.0 37.0 82-83 26.569450732203077 27.0 22.0 33.0 10.5 37.0 84-85 26.526181127044985 27.0 22.0 33.0 10.5 37.0 86-87 26.306709166782156 27.0 22.0 33.0 10.5 37.0 88-89 26.29720163577049 27.0 22.0 33.0 10.5 37.0 90-91 26.4514189803052 27.0 22.0 33.0 15.0 37.0 92-93 26.355633795071128 27.0 22.0 33.0 15.0 37.0 94-95 26.21551540189227 27.0 22.0 33.0 10.5 37.0 96-97 26.293751691095434 27.0 22.0 33.0 10.5 37.0 98-99 26.37038762657093 27.0 22.0 33.0 15.0 37.0 100 26.045677945035823 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.509959370845703 1101 2 11.443780991530549 1101 3 8.549576483043303 1101 4 13.033384475845036 1101 5 10.963437260097752 1101 6 10.180270551723492 1101 7 9.677024097230511 1101 8 9.102793807102515 1101 9 7.4559184159423495 1101 10-11 7.900682669721029 1101 12-13 9.25106223339591 1101 14-15 8.06705136687572 1101 16-17 8.23007963476925 1101 18-19 8.356144711209883 1101 20-21 7.348923489931931 1101 22-23 7.5008430846909775 1101 24-25 7.628926210139415 1101 26-27 8.874396926119147 1101 28-29 8.406497186259994 1101 30-31 5.650457942507302 1101 32-33 7.215964378071764 1101 34-35 9.051408884108351 1101 36-37 8.37806712074299 1101 38-39 8.52865529974282 1101 40-41 9.314538817038166 1101 42-43 8.861090725282804 1101 44-45 8.669678026718842 1101 46-47 7.493632527455215 1101 48-49 5.501457426453388 1101 50-51 6.90355258797025 1101 52-53 6.07471327757581 1101 54-55 4.411319549313415 1101 56-57 6.399207643039578 1101 58-59 6.360143378985086 1101 60-61 7.031560198791528 1101 62-63 6.5111137690362995 1101 64-65 7.016424275989834 1101 66-67 5.894192891063788 1101 68-69 6.929761645638205 1101 70-71 6.49093825302451 1101 72-73 5.752403269529616 1101 74-75 5.144094098370939 1101 76-77 5.237315747654755 1101 78-79 5.332971008303456 1101 80-81 5.12990478606719 1101 82-83 4.77670991252338 1101 84-85 4.46205168503678 1101 86-87 4.311846542554072 1101 88-89 4.221360789324361 1101 90-91 4.010266292518576 1101 92-93 3.965659791473456 1101 94-95 3.9153729154971266 1101 96-97 3.937442589515932 1101 98-99 3.6886816520314376 1101 100 4.059661563242177 1102 1 6.961325852238517 1102 2 10.785642299827625 1102 3 8.291989102482486 1102 4 12.785108841091269 1102 5 10.802510960231675 1102 6 9.679766426431183 1102 7 6.722635639722064 1102 8 8.016512052066467 1102 9 6.646477602957916 1102 10-11 7.669156710564543 1102 12-13 9.359236401224873 1102 14-15 6.438572885011045 1102 16-17 7.671276437927926 1102 18-19 7.752748284150183 1102 20-21 7.163360625195718 1102 22-23 6.792144668192584 1102 24-25 7.258322213914287 1102 26-27 8.723145286757827 1102 28-29 7.967097001680056 1102 30-31 4.5017421956177515 1102 32-33 4.614048580526813 1102 34-35 5.425253611436588 1102 36-37 5.583341614015538 1102 38-39 4.937460491574118 1102 40-41 6.478755722756674 1102 42-43 7.169499954973798 1102 44-45 6.580818918115057 1102 46-47 5.6481931612722 1102 48-49 3.9105831177607833 1102 50-51 5.190273240800362 1102 52-53 6.07605894168195 1102 54-55 5.456647369535325 1102 56-57 6.579127461790403 1102 58-59 6.35142700757693 1102 60-61 6.221187118318838 1102 62-63 4.404472100461383 1102 64-65 6.279583316590482 1102 66-67 4.12021111659827 1102 68-69 6.425280724681446 1102 70-71 5.521941264591984 1102 72-73 5.342576295877361 1102 74-75 4.517036396321821 1102 76-77 3.6068528579378025 1102 78-79 4.749678442156526 1102 80-81 4.6705324966513935 1102 82-83 4.093767857440838 1102 84-85 3.2515099845420217 1102 86-87 3.466333708696837 1102 88-89 3.638169360495194 1102 90-91 3.576174862995501 1102 92-93 3.470455226936398 1102 94-95 3.3311570159474755 1102 96-97 3.5060846432106523 1102 98-99 3.4059339783632225 1102 100 3.534213124191723 1103 1 6.671603396399121 1103 2 10.930643611098223 1103 3 7.991336841010259 1103 4 11.963350514175158 1103 5 10.379988096472342 1103 6 9.267500689966663 1103 7 8.272899911180811 1103 8 8.170838340711562 1103 9 7.3183597875556 1103 10-11 7.530877487142817 1103 12-13 8.927881938696256 1103 14-15 8.705817397674029 1103 16-17 8.133418542792011 1103 18-19 7.319208399611558 1103 20-21 8.40532622329859 1103 22-23 6.994648760261164 1103 24-25 5.4472592638823905 1103 26-27 6.165198517387715 1103 28-29 7.138025906466506 1103 30-31 6.278271078004778 1103 32-33 8.13588784449011 1103 34-35 7.3321940936641745 1103 36-37 4.151769678823609 1103 38-39 6.42043945142002 1103 40-41 6.982473540106767 1103 42-43 7.8489988130901445 1103 44-45 5.874339710971171 1103 46-47 6.419628696496687 1103 48-49 5.678371964239165 1103 50-51 6.507630898377176 1103 52-53 6.118032209152631 1103 54-55 5.0454093457488085 1103 56-57 6.012384223181588 1103 58-59 5.5160478138587905 1103 60-61 5.2102562692346055 1103 62-63 3.001780900165322 1103 64-65 5.232625481766053 1103 66-67 4.220782875794725 1103 68-69 5.308985457150936 1103 70-71 3.9341543067606395 1103 72-73 4.653127693858771 1103 74-75 3.5473785718623496 1103 76-77 3.652796979723881 1103 78-79 3.1832640646483306 1103 80-81 4.290968005441776 1103 82-83 3.663018857589009 1103 84-85 3.4306388521816054 1103 86-87 2.986299629574006 1103 88-89 3.2559782412405234 1103 90-91 2.9159476827559665 1103 92-93 2.5057497967457323 1103 94-95 3.1876812306285593 1103 96-97 3.242816202120089 1103 98-99 2.9561179910182 1103 100 3.061616071037612 1104 1 6.429341142240954 1104 2 9.885491826112105 1104 3 7.479530272580568 1104 4 12.290863343330816 1104 5 9.703533521711467 1104 6 8.321792756138887 1104 7 4.1685776381705 1104 8 6.740679578050852 1104 9 6.819562548243589 1104 10-11 7.314700789040735 1104 12-13 7.567297040855227 1104 14-15 7.368822491533987 1104 16-17 7.2596871949895 1104 18-19 6.91953679834462 1104 20-21 7.570408387298269 1104 22-23 6.505278075805016 1104 24-25 6.100456104660157 1104 26-27 8.295903558289453 1104 28-29 7.302673606529051 1104 30-31 6.442910990093431 1104 32-33 7.791312789598013 1104 34-35 8.280511917553763 1104 36-37 6.8555739990665 1104 38-39 7.381239297340645 1104 40-41 7.323685721618009 1104 42-43 7.398176105528396 1104 44-45 6.809293940436042 1104 46-47 6.678275666414809 1104 48-49 5.457769903103749 1104 50-51 5.669324112409253 1104 52-53 6.023703613007555 1104 54-55 4.822474086388276 1104 56-57 6.073569565059639 1104 58-59 4.002146280450482 1104 60-61 4.329985254743374 1104 62-63 4.068530618135661 1104 64-65 3.066115493383265 1104 66-67 3.4653149743229257 1104 68-69 4.285303322343037 1104 70-71 2.62202530746103 1104 72-73 2.5511100923377157 1104 74-75 3.1986126244544444 1104 76-77 3.869198566510441 1104 78-79 4.3927473929588565 1104 80-81 3.2208075049253218 1104 82-83 3.092872357924506 1104 84-85 2.9628071658734925 1104 86-87 0.9826060735711195 1104 88-89 0.3285130237320395 1104 90-91 2.3100788843895863 1104 92-93 2.6320585701345927 1104 94-95 2.611783312240224 1104 96-97 2.8611055062742494 1104 98-99 2.4795977260100734 1104 100 0.9159890971362508 1105 1 5.8839769214153606 1105 2 -2.771655265978879 1105 3 0.5045173724076939 1105 4 8.573212657025767 1105 5 8.68004173224546 1105 6 8.256504406560456 1105 7 5.450966187414288 1105 8 6.100766799864864 1105 9 5.2245633765496144 1105 10-11 5.153791641926453 1105 12-13 5.359662714585085 1105 14-15 6.17272302030236 1105 16-17 6.388924931502576 1105 18-19 6.5319989598191235 1105 20-21 7.310981947611236 1105 22-23 2.2860740184529504 1105 24-25 4.332633653841317 1105 26-27 7.080507925245669 1105 28-29 6.153905087548164 1105 30-31 5.517388978950528 1105 32-33 7.368095767869445 1105 34-35 6.389956112317439 1105 36-37 6.494333291748632 1105 38-39 4.329354643513117 1105 40-41 5.62309833770966 1105 42-43 5.787436543175179 1105 44-45 6.366755788818779 1105 46-47 5.522114020709434 1105 48-49 5.164904471524597 1105 50-51 6.10755289291291 1105 52-53 5.784144232184147 1105 54-55 5.63904135633123 1105 56-57 5.520349515456392 1105 58-59 2.215215721030816 1105 60-61 4.580723200116392 1105 62-63 -0.6183689095317462 1105 64-65 -0.10006806081846875 1105 66-67 1.002565957391397 1105 68-69 3.0731999085332298 1105 70-71 3.2032571610196925 1105 72-73 2.907404544431113 1105 74-75 2.7040616099182735 1105 76-77 1.8061359426113128 1105 78-79 3.3368230628761317 1105 80-81 1.74080515079239 1105 82-83 1.111594253976456 1105 84-85 1.5980059315786086 1105 86-87 -0.19218968359967192 1105 88-89 1.688983287545529 1105 90-91 1.4031955423768707 1105 92-93 1.6536565508116787 1105 94-95 1.0793260017063098 1105 96-97 2.1071716471052966 1105 98-99 2.025478151909976 1105 100 2.181104889563553 1106 1 6.141018265837403 1106 2 10.304335292302241 1106 3 6.774804055207632 1106 4 11.780153977752878 1106 5 9.92860417486941 1106 6 9.376500211391889 1106 7 6.683837145841977 1106 8 7.437493428956287 1106 9 7.083362677872778 1106 10-11 5.894640671534759 1106 12-13 8.189482788466659 1106 14-15 8.01847690115699 1106 16-17 7.863537234481793 1106 18-19 7.2760673281186605 1106 20-21 8.453480494192501 1106 22-23 6.391887563927156 1106 24-25 5.242368581338706 1106 26-27 7.880192316499517 1106 28-29 6.249444160234642 1106 30-31 5.537376820730529 1106 32-33 7.480679784536182 1106 34-35 7.123981518983353 1106 36-37 6.18935696726011 1106 38-39 7.329765355694779 1106 40-41 6.790209769932439 1106 42-43 6.339993459570909 1106 44-45 4.594955219881413 1106 46-47 6.063699729686451 1106 48-49 6.033763049025804 1106 50-51 6.800600566342343 1106 52-53 5.772098336522134 1106 54-55 5.492027889063255 1106 56-57 5.209372675635439 1106 58-59 5.235110013141444 1106 60-61 5.099839120133691 1106 62-63 5.1849821108394245 1106 64-65 5.3761929822506325 1106 66-67 3.3223461771716174 1106 68-69 3.345598871653298 1106 70-71 3.032509527599739 1106 72-73 2.9320094670211887 1106 74-75 3.05995977090657 1106 76-77 3.838410190102948 1106 78-79 4.368325541780518 1106 80-81 3.813947785228766 1106 82-83 2.0773137059631424 1106 84-85 2.7595394081173907 1106 86-87 3.385200494267707 1106 88-89 3.3204693224249873 1106 90-91 3.1806130406321245 1106 92-93 2.9936712727458072 1106 94-95 1.4912881234775526 1106 96-97 1.5753038389384457 1106 98-99 2.0385233781577483 1106 100 2.764291715698416 1107 1 6.156455476685242 1107 2 10.47774096160163 1107 3 6.237910156477987 1107 4 11.438571021435617 1107 5 9.651603433617463 1107 6 8.229945754934054 1107 7 3.637967708426647 1107 8 6.365921719220328 1107 9 5.812068298607386 1107 10-11 4.68686902825587 1107 12-13 6.193111088954868 1107 14-15 6.192201436596498 1107 16-17 7.15863922052581 1107 18-19 6.400804694885014 1107 20-21 5.53605915636053 1107 22-23 5.871421342011658 1107 24-25 4.610643636932604 1107 26-27 7.282322137832239 1107 28-29 6.485022336754849 1107 30-31 5.348989554927805 1107 32-33 5.07673581295936 1107 34-35 6.94039002548848 1107 36-37 6.852849030752175 1107 38-39 6.439617616513221 1107 40-41 7.823287499145433 1107 42-43 6.218971298693678 1107 44-45 4.117892494630283 1107 46-47 3.382758211909181 1107 48-49 4.099796456484338 1107 50-51 5.02217738816071 1107 52-53 3.8635528517689224 1107 54-55 5.379500092115169 1107 56-57 5.437542166727635 1107 58-59 3.367822867472654 1107 60-61 2.919302095876862 1107 62-63 0.5051326616663019 1107 64-65 2.3165547898829715 1107 66-67 4.399242120734229 1107 68-69 5.179352673764587 1107 70-71 4.471992057058117 1107 72-73 2.5035358070257914 1107 74-75 2.6943487492569 1107 76-77 3.1911074525828234 1107 78-79 4.091813643408717 1107 80-81 2.226025079914759 1107 82-83 1.820571508278917 1107 84-85 2.352847692246929 1107 86-87 2.347319963633552 1107 88-89 2.7247788078938946 1107 90-91 1.751819875486298 1107 92-93 2.526546435693433 1107 94-95 2.5034714938734837 1107 96-97 2.4403768139148028 1107 98-99 1.9095723774676152 1107 100 -0.00694898670170474 1108 1 4.317306799764996 1108 2 -3.3334103821692036 1108 3 0.005482632740715587 1108 4 6.657412550736492 1108 5 7.107701555327004 1108 6 6.92064971152876 1108 7 4.1744242456591 1108 8 4.952725406830968 1108 9 4.916003052728357 1108 10-11 3.463774332069928 1108 12-13 2.738730327063241 1108 14-15 4.135439476001512 1108 16-17 4.699771367570431 1108 18-19 4.065240173193864 1108 20-21 4.920216109713632 1108 22-23 4.217265883014484 1108 24-25 3.090119807703001 1108 26-27 4.713781203974733 1108 28-29 6.186003438899821 1108 30-31 4.70289384717784 1108 32-33 4.712778528548938 1108 34-35 3.200719473496388 1108 36-37 1.4317441269657074 1108 38-39 2.709775328539333 1108 40-41 5.463575335088141 1108 42-43 3.093414363732645 1108 44-45 3.7488342837191837 1108 46-47 4.849001286144187 1108 48-49 5.001897176074191 1108 50-51 2.046966454073303 1108 52-53 0.38532964097277045 1108 54-55 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2213 50-51 -23.470620965748754 2213 52-53 -17.905573984734414 2213 54-55 -17.840404807861127 2213 56-57 -17.60159123468625 2213 58-59 -18.11737705836817 2213 60-61 -19.344869296917544 2213 62-63 -17.672415952104203 2213 64-65 -17.751885466391684 2213 66-67 -8.917802940082375 2213 68-69 -22.76961851965105 2213 70-71 -17.447760877396117 2213 72-73 -9.5695713104314 2213 74-75 -4.609892304532817 2213 76-77 -3.431526444238667 2213 78-79 -4.892653200753681 2213 80-81 -3.506684586916034 2213 82-83 -3.5977879023380055 2213 84-85 -3.061323092685388 2213 86-87 -3.5179398062298617 2213 88-89 -3.1963620276733344 2213 90-91 -3.0535990630649223 2213 92-93 -4.01137458478544 2213 94-95 -4.048619855641139 2213 96-97 -3.656507892882921 2213 98-99 -4.118820860674514 2213 100 -3.745288976124904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 655.0 3 645.0 4 3727.0 5 11845.0 6 17888.0 7 22801.0 8 22552.0 9 23666.0 10 25756.0 11 29304.0 12 35566.0 13 46152.0 14 35898.0 15 39713.0 16 45097.0 17 47652.0 18 48274.0 19 47666.0 20 46994.0 21 45490.0 22 47188.0 23 51728.0 24 57172.0 25 62635.0 26 69149.0 27 73647.0 28 79824.0 29 82876.0 30 87469.0 31 95583.0 32 109203.0 33 130714.0 34 156690.0 35 181316.0 36 192947.0 37 155504.0 38 41845.0 39 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40249371452194 18.964033446566916 11.569094862183107 26.064377976728036 2 16.352590493104078 20.8115453265286 37.98620516454243 24.849659015824894 3 18.864263388363433 25.117648226368516 28.820625560595335 27.19746282467272 4 13.181624183329593 16.0830720362937 37.07560812606662 33.659695654310084 5 13.757553213558332 36.67599332486508 35.637832032267916 13.928621429308667 6 32.80234537582427 36.8804300516645 17.180651244320227 13.136573328191009 7 27.954227561094203 30.822786461983416 22.52827186617033 18.694714110752056 8 26.242213851583013 34.5523936329641 21.102880722615005 18.102511792837888 9 25.742749290755203 15.478558533211414 20.50376310051347 38.27492907551992 10-11 24.46088183830018 26.20682473539442 28.18939175685429 21.14290166945111 12-13 25.32107562596887 23.75574130694173 28.147061747419926 22.776121319669475 14-15 22.639048381199455 24.992878436289708 25.319929488706585 27.04814369380425 16-17 22.435905028658144 28.510257395145942 26.31968766717051 22.7341499090254 18-19 22.262097702308605 27.725136027222053 27.814689753525556 22.198076516943782 20-21 23.560105433936414 27.3699019150473 26.589562892912667 22.48042975810362 22-23 22.502837003841773 27.4086315459778 27.166410438857337 22.92212101132309 24-25 22.95470536654011 27.17209712900231 26.582303377692547 23.290894126765032 26-27 22.82493696227733 26.999325611473914 26.865334287670656 23.3104031385781 28-29 22.605206474710663 26.994804317578247 27.327258884053656 23.07273032365743 30-31 23.335605470765255 27.651135418544566 26.40696128125468 22.606297829435498 32-33 22.274289825558352 27.39293575352572 27.313427921638443 23.01934649927748 34-35 22.395367796089474 26.70217390366653 27.265790983372817 23.636667316871176 36-37 23.184661652625103 27.40241545350789 26.658702354310353 22.754220539556655 38-39 22.86474363697855 27.100160787803556 26.897900689441474 23.13719488577642 40-41 22.948790487675193 27.15054791394228 26.599406721886332 23.3012548764962 42-43 22.730909540841978 27.20723656165001 27.136726205842265 22.925127691665743 44-45 23.171611400872507 26.63760053337526 27.057013625818822 23.133774439933408 46-47 22.872384749122816 27.288104713136267 27.296366930979175 22.543143606761742 48-49 22.773682772425918 26.94132680014944 27.35272770632715 22.932262721097487 50-51 23.042461264504823 26.98858977951979 26.89979114998971 23.069157805985675 52-53 22.317126837033623 27.154785140942295 27.11605024853728 23.4120377734868 54-55 22.861651548341175 27.132786864559467 26.78035049215372 23.22521109494564 56-57 23.040433533959842 26.962721751982414 26.750653935922315 23.24619077813543 58-59 22.602754000744323 26.403801780653286 27.46007844035384 23.533365778248548 60-61 23.21407096373748 27.063924239238148 26.671797157563393 23.050207639460986 62-63 22.45947661461307 27.113839123994065 27.23235746699736 23.194326794395508 64-65 22.49402732591139 26.541673313276032 27.265396329587738 23.698903031224845 66-67 22.79867066242991 26.832505373526512 26.927714317763083 23.441109646280495 68-69 23.31334122544702 26.603319363170012 26.897496965650603 23.18584244573237 70-71 23.21260210405513 26.794372100460293 26.611114954168702 23.381910841315868 72-73 22.85361990042975 26.96571495522968 27.090564214255036 23.090100930085537 74-75 23.259898247486966 26.40249710107117 26.7257237766387 23.611880874803166 76-77 23.078120490427683 26.977731397847165 26.84977089145671 23.094377220268445 78-79 23.091603096261895 26.713259156079456 27.067987737212256 23.127150010446396 80-81 23.207933591920234 26.630152220566465 26.788701194808805 23.373212992704488 82-83 22.603994948247937 26.908188865248086 27.20674182179419 23.281074364709788 84-85 22.9530875778203 26.44664171185936 26.670026767865007 23.93024394245533 86-87 23.198328104234186 26.790244649272033 26.719889094866943 23.29153815162684 88-89 22.903275448803466 26.25281349814832 27.155413596683033 23.688497456365177 90-91 23.32366472677109 26.373222913936022 26.736644621596877 23.566467737696012 92-93 22.786080500866422 26.688935440347194 27.070906562306252 23.45407749648013 94-95 22.722613607093255 26.228834900495595 27.04379468359786 24.004756808813283 96-97 23.108539290580925 26.430907762595073 26.900390915048405 23.560162031775597 98-99 23.117361539837088 26.166921194510333 26.970026721555378 23.745690544097197 100 23.275451083453035 26.082580404573374 26.682287112513603 23.95968139945999 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 298.0 2 276.5 3 195.5 4 227.0 5 320.0 6 448.5 7 633.5 8 922.5 9 1364.0 10 1916.5 11 2527.5 12 3265.0 13 4079.5 14 4902.5 15 5592.0 16 6240.0 17 7002.0 18 7680.5 19 8467.0 20 9251.5 21 9954.5 22 10691.0 23 11512.0 24 12523.5 25 13653.0 26 14922.0 27 16392.0 28 18234.5 29 20595.5 30 23657.5 31 27708.0 32 32800.0 33 38481.5 34 44662.5 35 51587.0 36 59021.0 37 66026.5 38 72302.0 39 77894.5 40 82602.5 41 85797.5 42 87510.0 43 88237.5 44 87629.0 45 86480.0 46 84766.5 47 82899.5 48 81947.0 49 81700.0 50 82557.5 51 79311.0 52 71270.5 53 64376.5 54 59026.5 55 54173.0 56 49453.0 57 44798.5 58 39850.0 59 35035.5 60 30412.5 61 25543.5 62 21079.0 63 17248.5 64 13855.5 65 11151.0 66 8829.0 67 6785.0 68 5218.5 69 3984.0 70 2967.0 71 2208.0 72 1691.0 73 1252.0 74 913.0 75 644.0 76 428.0 77 286.0 78 210.5 79 150.0 80 98.5 81 60.5 82 42.5 83 30.0 84 16.5 85 13.0 86 12.0 87 9.0 88 5.5 89 2.5 90 2.5 91 3.5 92 2.0 93 1.0 94 0.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 2.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.89990254890703 2 26.422664968421888 3 6.902133145030544 4 25.676317184609328 5 13.894854010343455 6 11.2492878150937 7 8.971528002340586 8 9.72548709047993 9 6.176375479281194 10-11 7.684799609315708 12-13 11.26655623675996 14-15 8.490541964464448 16-17 8.997199655951446 18-19 7.2954131992335896 20-21 9.182136752700803 22-23 6.448578599915968 24-25 6.050656969952946 26-27 10.656112031360333 28-29 7.053237334107662 30-31 4.700508373534659 32-33 9.803293978930325 34-35 8.201378394014787 36-37 8.00465037495573 38-39 6.857169254730118 40-41 9.683668912661384 42-43 7.37242816006617 44-45 7.384747034121081 46-47 6.546953608440188 48-49 4.436752480823253 50-51 7.299834795099727 52-53 5.272368105555152 54-55 5.862816138604932 56-57 5.532252352135015 58-59 3.948529104439854 60-61 4.6987925303627245 62-63 3.446556984691599 64-65 5.123199739543806 66-67 3.1208987498542635 68-69 5.8994647889182925 70-71 3.0085550180713483 72-73 1.8057489545455545 74-75 1.0104116483753385 76-77 1.2191725676273188 78-79 1.9751334728005858 80-81 2.6005143129917925 82-83 0.8387173412350112 84-85 1.3175475761515398 86-87 0.571309782285899 88-89 0.6532302947510598 90-91 0.2401740480920044 92-93 0.3335115170473419 94-95 0.5911739666994437 96-97 0.6048787140855325 98-99 0.4797101544918795 100 0.42508914685197774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2272935.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.26746566888923 #Duplication Level Percentage of deduplicated Percentage of total 1 97.32426913200437 92.71836468282963 2 2.0857637396166138 3.9741085091467947 3 0.3283683365694822 0.9384845769265022 4 0.10495010629433887 0.39993322593368824 5 0.04836829573036268 0.23039624764775044 6 0.030175119257968393 0.1724824282777885 7 0.019147185643728713 0.12768726956788418 8 0.01560433331337076 0.11892682305739627 9 0.008117187459431704 0.06959734898574133 >10 0.03263810251314579 0.5001107101288088 >50 9.24240509149531E-4 0.0641633521243156 >100 0.0013025392272281494 0.27066060836462724 >500 2.7876139558062054E-4 0.201168454500758 >1k 9.292046519327894E-5 0.21391576250828678 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2712 0.11931709441757024 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.399597876754065E-5 0.0 5 0.0 0.0 0.0 4.399597876754065E-5 0.0 6 0.0 0.0 0.0 4.399597876754065E-5 4.399597876754065E-5 7 0.0 0.0 0.0 4.399597876754065E-5 4.399597876754065E-5 8 0.0 0.0 0.0 4.399597876754065E-5 4.399597876754065E-5 9 0.0 0.0 0.0 4.399597876754065E-5 4.399597876754065E-5 10-11 0.0 0.0 0.0 4.399597876754065E-5 4.399597876754065E-5 12-13 0.0 0.0 0.0 6.599396815131097E-5 4.399597876754065E-5 14-15 0.0 0.0 0.0 8.79919575350813E-5 4.399597876754065E-5 16-17 0.0 0.0 0.0 8.79919575350813E-5 4.399597876754065E-5 18-19 0.0 0.0 0.0 1.3198793630262194E-4 4.399597876754065E-5 20-21 0.0 0.0 0.0 1.3198793630262194E-4 4.399597876754065E-5 22-23 2.1997989383770325E-5 0.0 0.0 1.5398592568639227E-4 4.399597876754065E-5 24-25 4.399597876754065E-5 0.0 0.0 1.9798190445393293E-4 4.399597876754065E-5 26-27 4.399597876754065E-5 0.0 0.0 3.519678301403252E-4 4.399597876754065E-5 28-29 4.399597876754065E-5 0.0 0.0 0.0017378411613178557 4.399597876754065E-5 30-31 8.79919575350813E-5 0.0 0.0 0.007787288241854694 8.79919575350813E-5 32-33 8.79919575350813E-5 0.0 0.0 0.020766101978279185 8.79919575350813E-5 34-35 8.79919575350813E-5 0.0 0.0 0.03981636078462429 8.79919575350813E-5 36-37 8.79919575350813E-5 0.0 0.0 0.0721974011575342 8.79919575350813E-5 38-39 8.79919575350813E-5 0.0 0.0 0.12017501600353728 8.79919575350813E-5 40-41 1.759839150701626E-4 0.0 0.0 0.18192337220378058 8.79919575350813E-5 42-43 1.759839150701626E-4 0.0 0.0 0.24048201994337717 8.79919575350813E-5 44-45 1.759839150701626E-4 0.0 0.0 0.308499803117995 8.79919575350813E-5 46-47 1.759839150701626E-4 0.0 0.0 0.39143222309480913 8.79919575350813E-5 48-49 1.759839150701626E-4 0.0 0.0 0.4813380057062784 8.79919575350813E-5 50-51 1.759839150701626E-4 0.0 0.0 0.5747414686297673 8.79919575350813E-5 52-53 1.759839150701626E-4 0.0 0.0 0.675822229848192 8.79919575350813E-5 54-55 1.759839150701626E-4 0.0 0.0 0.7866261023742429 8.79919575350813E-5 56-57 1.759839150701626E-4 0.0 0.0 0.9148083865134726 8.79919575350813E-5 58-59 1.759839150701626E-4 0.0 0.0 1.0589392129559358 8.79919575350813E-5 60-61 1.759839150701626E-4 0.0 0.0 1.2115832612899182 8.79919575350813E-5 62-63 1.759839150701626E-4 0.0 0.0 1.368670903479422 8.79919575350813E-5 64-65 1.759839150701626E-4 0.0 0.0 1.5437748989742337 8.79919575350813E-5 66-67 1.9798190445393293E-4 0.0 0.0 1.74661835908198 8.79919575350813E-5 68-69 2.1997989383770324E-4 0.0 0.0 1.9708658628601348 8.79919575350813E-5 70-71 2.1997989383770324E-4 0.0 0.0 2.2399452690024133 8.79919575350813E-5 72-73 2.1997989383770324E-4 0.0 0.0 2.514744152384472 8.79919575350813E-5 74-75 2.1997989383770324E-4 0.0 0.0 2.7949325431655545 8.79919575350813E-5 76-77 2.1997989383770324E-4 0.0 0.0 3.111923570185685 8.79919575350813E-5 78-79 2.1997989383770324E-4 0.0 0.0 3.458259915043765 8.79919575350813E-5 80-81 2.1997989383770324E-4 0.0 0.0 3.852485882790313 8.79919575350813E-5 82-83 2.1997989383770324E-4 4.399597876754065E-5 0.0 4.273835371447049 8.79919575350813E-5 84-85 2.1997989383770324E-4 4.399597876754065E-5 0.0 4.695052872167484 8.79919575350813E-5 86-87 2.1997989383770324E-4 8.79919575350813E-5 0.0 5.154678862351981 8.79919575350813E-5 88 2.1997989383770324E-4 1.3198793630262194E-4 0.0 5.540237622281324 8.79919575350813E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAT 800 0.0 18.042315 1 ATAGGAC 640 0.0 16.832106 3 GTACATG 9515 0.0 16.752487 1 GTATTAC 610 1.0913936E-11 16.460558 1 TATACGG 185 0.0021145141 16.349463 5 TACATGG 9615 0.0 16.3123 2 GTACAAG 820 0.0 16.071627 1 GTCCTAA 680 3.6379788E-12 15.68897 1 GTATAGG 585 1.8208084E-9 15.0185 1 GTATATA 935 0.0 14.7660885 1 CATGGGG 2995 0.0 14.765104 4 ACATGGG 9915 0.0 14.753389 3 GTCTAAG 645 4.6020432E-10 14.59439 1 TAGACAG 985 0.0 14.329986 5 GTACATA 880 0.0 14.262699 1 TATATAG 695 1.8189894E-12 13.781382 5 GTCCTAG 820 5.456968E-12 13.775681 1 GTGTTAA 780 4.0017767E-11 13.677562 1 TATACTG 860 0.0 13.48197 5 TAGTCCG 225 0.007768091 13.442891 5 >>END_MODULE