FastQCFastQC Report
Wed 25 May 2016
SRR1295081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272935
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82760.3641107202801664No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53840.23687434968443885No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25070.11029791877022439No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1350.00998174317.4110415
GGGTACG2052.213216E-417.11431
CGTAGTA2403.9034185E-516.6974012
GTACATG115300.016.388041
TAGTACG2304.670934E-516.3512364
CATGGGG36200.016.2326684
TACATGG115750.015.7092612
ACATGGG115250.015.0509633
CCTAGTA6001.8189894E-1215.0276592
GTATAGA6750.014.8505031
GTACGGA2902.0814603E-514.5715886
CTAGACT9450.014.426294
CTACACT11800.014.3419754
GTATTGC6455.456968E-1213.98710251
ATAGCGA2350.001027427613.9838138
CGTAAGA2150.00599616813.97921852
GTATAGG6102.7284841E-1113.9679951
TCTACAC9900.013.77024653
CTATACA9300.013.6480084
TGGGGCG3154.4837394E-513.4313755
Produced by FastQC (version 0.11.5)