##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295080_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 215668 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.184362075041268 27.0 22.0 33.0 6.0 33.0 2 21.38178125637554 27.0 2.0 33.0 2.0 33.0 3 23.96692137915685 27.0 15.0 33.0 6.0 33.0 4 23.64319695086893 33.0 6.0 37.0 2.0 37.0 5 25.987842424467235 33.0 15.0 37.0 2.0 37.0 6 26.55121297549938 33.0 15.0 37.0 2.0 37.0 7 26.66231429790233 33.0 15.0 37.0 6.0 37.0 8 27.362302242335442 33.0 15.0 37.0 6.0 37.0 9 27.978179423929372 33.0 15.0 37.0 6.0 37.0 10-11 27.72211686481073 33.0 15.0 37.0 6.0 37.0 12-13 26.650096444535116 33.0 15.0 37.0 4.0 37.0 14-15 28.331599959196545 33.0 15.0 37.0 6.0 40.0 16-17 28.649853014819072 33.0 22.0 37.0 6.0 40.0 18-19 28.75403629652985 33.0 18.5 37.0 6.0 40.0 20-21 28.27417836674889 33.0 18.5 37.0 6.0 40.0 22-23 29.14318535897769 33.0 22.0 37.0 6.0 40.0 24-25 29.359485412764062 33.0 24.5 37.0 6.0 40.0 26-27 27.810859747389507 33.0 15.0 37.0 4.0 40.0 28-29 28.542736984624515 33.0 22.0 37.0 6.0 40.0 30-31 29.16984670883024 33.0 22.0 37.0 6.0 40.0 32-33 27.726964593727395 33.0 22.0 37.0 6.0 40.0 34-35 27.38928816514272 33.0 15.0 37.0 6.0 40.0 36-37 27.76020318266966 33.0 22.0 37.0 6.0 40.0 38-39 27.747795222286108 33.0 18.5 37.0 6.0 40.0 40-41 26.774162138101154 33.0 15.0 37.0 4.0 40.0 42-43 27.201678042175935 33.0 15.0 37.0 6.0 40.0 44-45 27.052182057607062 33.0 15.0 37.0 6.0 40.0 46-47 26.61968395867723 33.0 15.0 37.0 6.0 40.0 48-49 26.44639445814864 30.0 15.0 37.0 6.0 40.0 50-51 26.57994231874919 33.0 15.0 37.0 6.0 40.0 52-53 26.93288758647551 33.0 18.5 37.0 6.0 40.0 54-55 26.826089174100932 33.0 22.0 37.0 6.0 40.0 56-57 26.568566036686015 33.0 15.0 37.0 6.0 40.0 58-59 26.487462210434558 27.0 15.0 37.0 6.0 40.0 60-61 26.347735871802957 27.0 18.5 37.0 6.0 37.0 62-63 26.00922250867073 27.0 15.0 37.0 6.0 37.0 64-65 25.746522432627927 27.0 15.0 37.0 6.0 37.0 66-67 25.973797689040563 27.0 18.5 35.0 6.0 37.0 68-69 25.471314705936905 27.0 18.5 33.0 6.0 37.0 70-71 25.344733571971737 27.0 15.0 33.0 6.0 37.0 72-73 25.12541730808465 27.0 15.0 33.0 6.0 37.0 74-75 25.183559916167443 27.0 15.0 33.0 6.0 37.0 76-77 24.768876235695608 27.0 15.0 33.0 6.0 37.0 78-79 24.324102323942355 27.0 15.0 33.0 6.0 37.0 80-81 24.291640391713187 27.0 15.0 33.0 6.0 37.0 82-83 24.563887549381455 27.0 15.0 33.0 6.0 37.0 84-85 24.364636849231225 27.0 15.0 33.0 6.0 37.0 86-87 23.857282953428417 27.0 15.0 33.0 6.0 33.0 88-89 23.79583433796391 27.0 15.0 33.0 6.0 33.0 90-91 23.959878146039284 27.0 15.0 33.0 6.0 35.0 92-93 23.664683680471835 27.0 15.0 33.0 6.0 33.0 94-95 23.634688502698594 27.0 15.0 33.0 6.0 33.0 96-97 23.831375076506482 27.0 15.0 33.0 6.0 37.0 98-99 23.8319453975555 27.0 15.0 33.0 6.0 37.0 100 23.254659940278575 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.843408811748443 1101 2 10.783092022595007 1101 3 7.436174479097222 1101 4 11.49622169429496 1101 5 9.95349544200285 1101 6 9.12014317434043 1101 7 8.76808391402328 1101 8 8.152365999136943 1101 9 6.51062245857171 1101 10-11 7.097400492470278 1101 12-13 8.547374897294578 1101 14-15 7.517018110818501 1101 16-17 7.088877431577192 1101 18-19 7.163878027189728 1101 20-21 6.100295848326262 1101 22-23 6.156251522575168 1101 24-25 5.979955674929336 1101 26-27 7.476862866675585 1101 28-29 7.017395034873466 1101 30-31 4.641000200425108 1101 32-33 6.395121228020027 1101 34-35 7.961101305529205 1101 36-37 7.742723268122393 1101 38-39 7.326768703304435 1101 40-41 7.832078445288467 1101 42-43 7.2089045762847945 1101 44-45 7.625173691957048 1101 46-47 6.806509467884151 1101 48-49 5.1592265185982775 1101 50-51 6.560150354622756 1101 52-53 5.079675028163727 1101 54-55 3.455365609881369 1101 56-57 5.234214724652105 1101 58-59 5.518448806507617 1101 60-61 6.400667895804407 1101 62-63 5.529494424224051 1101 64-65 5.859476667038713 1101 66-67 5.4431240649713395 1101 68-69 6.335603364626223 1101 70-71 5.582296561247201 1101 72-73 4.450951791771928 1101 74-75 4.087575837274347 1101 76-77 4.223948795543976 1101 78-79 4.518593155268494 1101 80-81 3.787890273436613 1101 82-83 3.767611422109564 1101 84-85 3.526943579997269 1101 86-87 3.2005149065430096 1101 88-89 3.3322453499029656 1101 90-91 3.4444597538738755 1101 92-93 2.918072227589402 1101 94-95 3.209950540693402 1101 96-97 3.4643237761358776 1101 98-99 2.932129030470584 1101 100 2.537439168677139 1102 1 6.065742049925245 1102 2 9.942488730178983 1102 3 7.493991588241766 1102 4 11.130021294637523 1102 5 10.062835055667325 1102 6 8.417107680953812 1102 7 6.178555890424459 1102 8 6.7769782362670234 1102 9 5.397576497966515 1102 10-11 6.270397447461235 1102 12-13 8.215333313889875 1102 14-15 5.691206901378973 1102 16-17 6.576105046002919 1102 18-19 6.690376742576536 1102 20-21 6.361771726145275 1102 22-23 5.821005542938671 1102 24-25 6.115123150426534 1102 26-27 7.852802537433984 1102 28-29 6.646182264866809 1102 30-31 3.0982225812415543 1102 32-33 3.121960128010514 1102 34-35 4.566113675878427 1102 36-37 4.814775794528334 1102 38-39 4.423792682750626 1102 40-41 5.879930286567372 1102 42-43 6.405605023520497 1102 44-45 6.103368572535597 1102 46-47 4.765708725663199 1102 48-49 3.5422791401607476 1102 50-51 4.680504336923644 1102 52-53 5.137646766673576 1102 54-55 4.709412141425759 1102 56-57 5.703900232312208 1102 58-59 5.680926206831153 1102 60-61 5.227230841850652 1102 62-63 4.399884090224624 1102 64-65 5.3768165661727885 1102 66-67 3.2666776856774007 1102 68-69 5.471415525631073 1102 70-71 4.732420264739449 1102 72-73 4.813736071912761 1102 74-75 3.8869263939400653 1102 76-77 3.395684926479362 1102 78-79 4.329279233867791 1102 80-81 4.281326851607705 1102 82-83 3.668831804638824 1102 84-85 2.2676563024256637 1102 86-87 2.7029889763879034 1102 88-89 2.9949225789447382 1102 90-91 2.7996338903283444 1102 92-93 2.5212623503413276 1102 94-95 2.891597224488244 1102 96-97 3.226361077496616 1102 98-99 3.0016884557251267 1102 100 3.3018458676970255 1103 1 6.71713546709649 1103 2 10.594736052023418 1103 3 7.327402549272659 1103 4 11.916312243247983 1103 5 9.886658996049253 1103 6 8.516238250741115 1103 7 7.600540054374154 1103 8 7.371819875763997 1103 9 6.634632079397967 1103 10-11 6.906653463607796 1103 12-13 7.886318021233453 1103 14-15 8.169868988011483 1103 16-17 7.623972225012391 1103 18-19 6.575196646318194 1103 20-21 7.781669645722982 1103 22-23 6.830883667453499 1103 24-25 4.946700157102512 1103 26-27 5.959036041548249 1103 28-29 6.88883674398496 1103 30-31 5.541806652038012 1103 32-33 7.965006627001355 1103 34-35 7.215013303265479 1103 36-37 4.080039340561559 1103 38-39 6.00291471349119 1103 40-41 6.494217664303747 1103 42-43 7.032652454043706 1103 44-45 4.8654396795065615 1103 46-47 5.684296677844539 1103 48-49 5.253736988320977 1103 50-51 6.092387196728023 1103 52-53 5.849298684077709 1103 54-55 5.094697811352788 1103 56-57 5.467038720124659 1103 58-59 4.9120820832478564 1103 60-61 4.405046786579732 1103 62-63 1.9427442827413124 1103 64-65 4.439442711181329 1103 66-67 3.164820443013216 1103 68-69 4.582985933952763 1103 70-71 2.946197240364352 1103 72-73 3.9272180622869257 1103 74-75 2.9978757806812553 1103 76-77 3.3214728476096234 1103 78-79 2.5842765168983775 1103 80-81 3.3604779927348574 1103 82-83 2.803739039540236 1103 84-85 2.6960932687352397 1103 86-87 2.3765548046754397 1103 88-89 2.509332220304774 1103 90-91 2.393320817824076 1103 92-93 2.2181358948220016 1103 94-95 2.6359692164149635 1103 96-97 2.95689593233282 1103 98-99 2.8249204849131395 1103 100 2.8319071935781004 1104 1 6.563578391588337 1104 2 9.94515919136958 1104 3 7.3046032603013344 1104 4 11.875118951648947 1104 5 9.849909633656491 1104 6 8.433851667543031 1104 7 4.36514705646087 1104 8 6.564696064032024 1104 9 6.464864323002576 1104 10-11 6.674178001825588 1104 12-13 7.141510655903833 1104 14-15 6.918927068234066 1104 16-17 6.915122043390941 1104 18-19 6.962378808772787 1104 20-21 7.899187421439802 1104 22-23 6.637640371990173 1104 24-25 6.355430545849714 1104 26-27 8.244259010865814 1104 28-29 7.316502135437155 1104 30-31 6.355132643227442 1104 32-33 7.754040748649086 1104 34-35 7.8842835328040834 1104 36-37 6.5124191612586735 1104 38-39 7.400201984532227 1104 40-41 7.2099028022587675 1104 42-43 7.412139856470478 1104 44-45 6.984981375155378 1104 46-47 6.492995186230036 1104 48-49 5.082394535272055 1104 50-51 5.263454319380468 1104 52-53 5.70374068114085 1104 54-55 4.12592124438471 1104 56-57 5.86303145526648 1104 58-59 4.249105809965531 1104 60-61 4.367741933036182 1104 62-63 3.517223570166923 1104 64-65 2.2821662601418353 1104 66-67 2.2452603920046386 1104 68-69 3.59022180998646 1104 70-71 2.2577311687977684 1104 72-73 2.0838327543033124 1104 74-75 3.0246939273695617 1104 76-77 3.6353978031703313 1104 78-79 4.12749372842941 1104 80-81 2.680107600896232 1104 82-83 2.862133235382263 1104 84-85 2.1562766291185795 1104 86-87 0.08475771834728363 1104 88-89 0.4647142050022026 1104 90-91 2.378622038938534 1104 92-93 2.207137170036468 1104 94-95 2.180070383429417 1104 96-97 1.945660520013476 1104 98-99 1.9982675364627234 1104 100 -0.024466946618204588 1105 1 5.279854765342105 1105 2 -2.301842895345004 1105 3 0.8754727247112548 1105 4 8.158768383598865 1105 5 8.445606364470308 1105 6 7.463255794600762 1105 7 5.497908475426787 1105 8 6.125767244185049 1105 9 4.6824390969418275 1105 10-11 4.839109603958676 1105 12-13 4.924432056321173 1105 14-15 5.838421619590431 1105 16-17 6.052963453082572 1105 18-19 6.2285299796515226 1105 20-21 7.048818768529994 1105 22-23 2.0139538428920964 1105 24-25 3.8133229641200543 1105 26-27 6.797632532776319 1105 28-29 5.893003410651623 1105 30-31 5.082596125722226 1105 32-33 7.137418907703108 1105 34-35 6.296460671686532 1105 36-37 5.803504252842256 1105 38-39 4.126598924017507 1105 40-41 5.394217664303749 1105 42-43 5.573748945271777 1105 44-45 6.346316872489023 1105 46-47 5.3876300111778725 1105 48-49 4.99900014621571 1105 50-51 5.871729301991181 1105 52-53 5.189386403375956 1105 54-55 4.870136407844019 1105 56-57 5.030898369247467 1105 58-59 2.38980138149347 1105 60-61 4.492722225176223 1105 62-63 0.03384077396938423 1105 64-65 0.44961814977781955 1105 66-67 1.3307414956447978 1105 68-69 3.0357052321983744 1105 70-71 3.091679696504702 1105 72-73 2.2735777114097324 1105 74-75 2.688051219277746 1105 76-77 1.8657710932236569 1105 78-79 2.8292326572492534 1105 80-81 1.6113990453664364 1105 82-83 0.9440460570840976 1105 84-85 1.5797336196124334 1105 86-87 0.2387039274824545 1105 88-89 1.7955164308310891 1105 90-91 1.4778383616837232 1105 92-93 1.7748464211377915 1105 94-95 1.4060569357132096 1105 96-97 2.1978608446135226 1105 98-99 2.0361924147377017 1105 100 2.263573860244769 1106 1 5.72635875921452 1106 2 10.010221881982929 1106 3 6.635897278820021 1106 4 10.821243490284495 1106 5 9.842069872512763 1106 6 8.942524861238251 1106 7 6.113752659692025 1106 8 6.850879400876153 1106 9 6.64904375828333 1106 10-11 5.594903454489366 1106 12-13 7.588047331515394 1106 14-15 7.660192053992443 1106 16-17 7.3645642846834 1106 18-19 6.695760530039966 1106 20-21 7.999625202494325 1106 22-23 5.914367454884655 1106 24-25 4.805234551821119 1106 26-27 7.287226580265102 1106 28-29 5.825424900967853 1106 30-31 5.365351350582706 1106 32-33 6.565279723984975 1106 34-35 6.434642091973217 1106 36-37 5.478676591172491 1106 38-39 6.426292544763761 1106 40-41 6.281612346435271 1106 42-43 5.406163870318281 1106 44-45 3.844859382610476 1106 46-47 5.336346500946991 1106 48-49 5.3366235185759265 1106 50-51 5.720649315121719 1106 52-53 5.050528760307785 1106 54-55 4.7107207168493765 1106 56-57 4.346438362682196 1106 58-59 4.552186762826221 1106 60-61 4.071273488990649 1106 62-63 4.077526078707319 1106 64-65 4.344939300888441 1106 66-67 2.85141771842628 1106 68-69 2.3941842780458416 1106 70-71 2.1308021387850395 1106 72-73 2.5025021013867566 1106 74-75 2.5151460874252436 1106 76-77 2.8442917191127037 1106 78-79 3.897150372535389 1106 80-81 3.2863656719120122 1106 82-83 1.3694624046057093 1106 84-85 2.6045328317800696 1106 86-87 2.9887137753870405 1106 88-89 2.5149605713278653 1106 90-91 2.598446743344738 1106 92-93 2.4923931986845673 1106 94-95 1.332904512034471 1106 96-97 1.2401827616722834 1106 98-99 1.4657842738032478 1106 100 2.6623089516114398 1107 1 6.160061692917452 1107 2 10.168341540102588 1107 3 6.321217214139956 1107 4 11.30351422255523 1107 5 9.634747165190053 1107 6 7.891069559782949 1107 7 4.169365965305328 1107 8 7.086788386785138 1107 9 6.020203379442954 1107 10-11 4.934993544579456 1107 12-13 6.4212651647332315 1107 14-15 6.351633495433884 1107 16-17 7.069288239407353 1107 18-19 6.611127820407258 1107 20-21 5.659621737490859 1107 22-23 5.727026267543465 1107 24-25 4.508919005505572 1107 26-27 7.564917732956257 1107 28-29 6.4188356193785765 1107 30-31 5.120866481097842 1107 32-33 4.825414859120112 1107 34-35 6.708406240737368 1107 36-37 6.900973888469789 1107 38-39 6.6253281187993345 1107 40-41 7.545443485266411 1107 42-43 6.021490735645145 1107 44-45 3.971996134747233 1107 46-47 3.2379404025408967 1107 48-49 3.791636223588629 1107 50-51 4.573184542656946 1107 52-53 3.828999538778561 1107 54-55 5.27086740199606 1107 56-57 5.496559637803472 1107 58-59 3.415174750814206 1107 60-61 2.874316917034072 1107 62-63 0.40239902858026966 1107 64-65 1.617692823641967 1107 66-67 3.784225526234092 1107 68-69 4.588946347807912 1107 70-71 4.221585229568131 1107 72-73 2.120341094225747 1107 74-75 2.386652208931366 1107 76-77 3.2196959695169554 1107 78-79 4.125465225850245 1107 80-81 2.444514205060546 1107 82-83 1.7597950449383468 1107 84-85 1.8220772993245369 1107 86-87 2.0023139139871766 1107 88-89 2.558561864929157 1107 90-91 0.9275931974911913 1107 92-93 1.4798036860950567 1107 94-95 1.6538504579804147 1107 96-97 1.8695140160035386 1107 98-99 1.5413790993980747 1107 100 -0.03825699895451251 1108 1 4.702474207028079 1108 2 -2.853832302825971 1108 3 0.037432340732440395 1108 4 6.295491323022894 1108 5 7.176353357739643 1108 6 6.768307102114832 1108 7 4.288419343792672 1108 8 5.131902078125243 1108 9 4.720130810502457 1108 10-11 2.8793170407897435 1108 12-13 2.867967309548579 1108 14-15 4.159366119204247 1108 16-17 4.883068274885506 1108 18-19 4.017654993443866 1108 20-21 5.077356780225902 1108 22-23 4.44658762861426 1108 24-25 3.099912393668774 1108 26-27 4.735434585076884 1108 28-29 6.242755148486779 1108 30-31 4.794689251618724 1108 32-33 4.820718036027063 1108 34-35 3.079455265088278 1108 36-37 1.5042435223998005 1108 38-39 2.495328925120898 1108 40-41 5.391657805537335 1108 42-43 2.8413181828310847 1108 44-45 3.510397199091692 1108 46-47 4.647471123392368 1108 48-49 4.645329176338187 1108 50-51 2.3838400820860706 1108 52-53 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-17.90097512639718 2212 20-21 -23.14486544199632 2212 22-23 -18.273037516102406 2212 24-25 -24.835098088511522 2212 26-27 -16.655980978614096 2212 28-29 -17.627774279843266 2212 30-31 -5.512297825573928 2212 32-33 -16.58230615121284 2212 34-35 -13.466791488564843 2212 36-37 -16.939582942758683 2212 38-39 -22.931295812824597 2212 40-41 -9.720039994769309 2212 42-43 -13.372181926684231 2212 44-45 -16.207092396953243 2212 46-47 -16.02832442715677 2212 48-49 -16.52316796061854 2212 50-51 -8.489849645377241 2212 52-53 -1.6799301716436865 2212 54-55 -22.493021486892825 2212 56-57 -22.173312157068324 2212 58-59 -11.65573671748532 2212 60-61 -5.678990265004451 2212 62-63 -1.8782330673503331 2212 64-65 -15.862534246137344 2212 66-67 -16.348779321322997 2212 68-69 -13.428960910770988 2212 70-71 -8.42498958864848 2212 72-73 -15.829540906029386 2212 74-75 -5.1196942638330185 2212 76-77 -8.562626393029287 2212 78-79 -4.061851396167871 2212 80-81 -1.28126395541911 2212 82-83 -2.179355356898622 2212 84-85 -3.985678792013644 2212 86-87 -4.318342419728857 2212 88-89 -2.437005171682653 2212 90-91 -3.7476683154568917 2212 92-93 -5.098586414683108 2212 94-95 -3.1511150988508128 2212 96-97 -3.668038605504311 2212 98-99 -2.8644124556472157 2212 100 -2.6144465639500467 2213 1 -20.425408392552633 2213 2 -16.45131657955553 2213 3 -19.497158854236112 2213 4 -18.232810563769558 2213 5 -20.77123574079285 2213 6 -21.282007363293975 2213 7 -10.393820847881484 2213 8 -10.506312956316204 2213 9 -4.555600446360874 2213 10-11 -17.26189290230699 2213 12-13 -15.792410048941985 2213 14-15 -23.11631522251483 2213 16-17 -17.514655594536478 2213 18-19 -17.95139761711818 2213 20-21 -17.16867496580584 2213 22-23 -19.265851225334025 2213 24-25 -7.782188035601472 2213 26-27 -22.78657799353947 2213 28-29 -23.691207115664163 2213 30-31 -18.904621919390557 2213 32-33 -22.645738987033734 2213 34-35 -17.245043087711963 2213 36-37 -23.070179957684058 2213 38-39 -10.89425877578756 2213 40-41 -16.274509709686782 2213 42-43 -22.360987896833485 2213 44-45 -16.209678671927577 2213 46-47 -15.877037212436722 2213 48-49 -5.39914521719281 2213 50-51 -22.23984964537724 2213 52-53 -17.08390237412335 2213 54-55 -16.765508259379597 2213 56-57 -16.23151321526938 2213 58-59 -16.81717160653215 2213 60-61 -17.94288902517187 2213 62-63 -16.247122745941503 2213 64-65 -16.131289675340533 2213 66-67 -7.927058560049996 2213 68-69 -21.30955792569636 2213 70-71 -15.675443667460767 2213 72-73 -8.31214771326306 2213 74-75 -3.071324999045842 2213 76-77 -2.5532486784276927 2213 78-79 -4.724707749322729 2213 80-81 -2.8343319487856107 2213 82-83 -2.740103204959901 2213 84-85 -2.6597094324621935 2213 86-87 -2.742649455976192 2213 88-89 -2.724812168444874 2213 90-91 -2.176141031243038 2213 92-93 -2.9042011979098312 2213 94-95 -3.3194401403101814 2213 96-97 -2.2916072701177477 2213 98-99 -3.0195692115502375 2213 100 -2.6838613945589067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60.0 3 77.0 4 554.0 5 1625.0 6 2249.0 7 2191.0 8 2098.0 9 2144.0 10 2492.0 11 3153.0 12 4029.0 13 4954.0 14 4662.0 15 5252.0 16 5367.0 17 5331.0 18 4993.0 19 5062.0 20 5148.0 21 5218.0 22 5594.0 23 6107.0 24 6512.0 25 6968.0 26 7399.0 27 7184.0 28 7542.0 29 8199.0 30 8738.0 31 9757.0 32 11154.0 33 12646.0 34 13965.0 35 14816.0 36 13598.0 37 7396.0 38 1433.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.258103789377564 22.89352204129255 10.271901790696495 22.57647237863339 2 11.68050297205087 29.65189893155863 31.114534669768663 27.553063426621843 3 22.43467745133024 36.52313548233615 23.019507553229452 18.02267951310416 4 13.893438316965693 27.061302942467076 24.52797537746519 34.51728336310204 5 10.208193059518846 46.111342674233434 30.314106469161135 13.36635779708658 6 29.79537919602155 33.223684210526315 18.323663489432242 18.657273104019893 7 20.82683623255908 39.60328871428282 20.358538709220145 19.21133634393795 8 26.437759494638176 38.36164955806312 19.592959576148143 15.607631371150566 9 26.35729763993101 24.985872999493886 16.64725739640609 32.00957196416901 10-11 20.59256030442178 37.02061460533139 20.165437906924893 22.221387183321937 12-13 25.738934772577625 33.913230574608946 20.377542397046852 19.97029225576657 14-15 26.917284915892125 32.51157096059714 19.1350125577291 21.436131565781633 16-17 20.50049781241212 35.97828372520755 26.147691949119267 17.373526513261066 18-19 23.558155437207702 34.65867402708247 25.42587758483861 16.357292950871223 20-21 20.94439536560352 34.462485149419685 24.634954965932515 19.95816451904428 22-23 18.58611067782804 35.68420348953807 20.052340595157627 25.677345237476263 24-25 20.76299041030123 37.02731648467987 17.58841849987074 24.621274605148155 26-27 23.69483822656028 31.392310274485503 19.742815090247117 25.1700364087071 28-29 22.87642292588358 30.800566684893372 24.084853606402547 22.2381567828205 30-31 25.44511684925376 34.22170224358351 21.432741141445 18.90043976571773 32-33 18.14657479175789 34.15785174544402 24.8433543047799 22.852219158018187 34-35 15.629794405802286 35.400695810775744 25.48526629499656 23.484243488425406 36-37 23.56707187353741 33.40491404058075 21.899691902620205 21.128322183261627 38-39 25.863793575356443 30.347358730000746 21.101296374629875 22.68755132001294 40-41 20.486961680278615 34.71430251765633 21.46349321618705 23.335242585878007 42-43 25.267723094265467 32.070907340625574 21.53556090918613 21.125808655922828 44-45 27.076231774917897 30.24509571674725 20.95395073149688 21.724721776837967 46-47 21.392573798680868 32.68821946590419 27.291265070689153 18.627941664725792 48-49 23.75599905080609 31.921972386205695 26.16264147105685 18.159387091931368 50-51 19.713025653767318 32.81023666066215 25.813528790608114 21.66320889496242 52-53 18.13912661838929 33.72369736425036 21.052105429985616 27.085070587374734 54-55 20.208271041551274 35.4970026419885 18.8164479714528 25.47827834500743 56-57 23.068777621719406 30.271566082550795 20.361695921483847 26.297960374245953 58-59 22.524993258080674 28.89803328581885 24.541067920021575 24.0359055360789 60-61 24.952711184927796 32.00487578642696 21.948323462779634 21.09408956586561 62-63 18.373999351904082 32.909350583886415 24.519148834027433 24.197501230182066 64-65 16.164541024448337 34.129038699920365 25.270748451735297 24.435671823896 66-67 22.686095480512794 32.20769192269468 22.02782286222273 23.0783897345698 68-69 25.446945920584866 29.860177038850054 21.00896954373173 23.683907496833342 70-71 20.892276025710053 33.29537495414024 21.169102196980162 24.64324682316954 72-73 24.77957843049475 31.584254996300782 21.49905516783141 22.137111405373055 74-75 26.2448132780083 29.93171791725598 20.787164092429027 23.0363047123067 76-77 21.56849384270888 32.13915642409532 25.797181814550523 20.49516791864528 78-79 23.933583141718948 31.797579508142427 25.026021706834715 19.242815643303903 80-81 20.706962633265807 32.39084329557667 24.646209592285086 22.25598447887244 82-83 19.130412408511553 33.34752393494467 21.024623812609207 26.497439843934572 84-85 20.939881774695273 34.195118742188015 19.192346368190062 25.672653114926646 86-87 23.14175154270564 30.232362743542108 20.76817872046454 25.857706993287717 88-89 22.959380546909166 29.046802758633593 24.19393689941371 23.799879795043534 90-91 24.713585812484865 31.61290773271735 21.943751047856782 21.729755406941003 92-93 19.9582868754961 32.24666609249047 23.71176975845028 24.083277273563144 94-95 18.132490789535048 33.24651401389731 24.363195448398077 24.257799748169564 96-97 23.358491357903794 31.814292276090082 21.796315596809432 23.03090076919669 98-99 25.044326757518075 29.907572012050355 21.113283115757493 23.934818114674076 100 21.11933895396553 32.32531895844214 23.36883533537268 23.186506752219653 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4306.0 1 4168.5 2 3400.5 3 2180.0 4 1257.5 5 753.0 6 494.5 7 382.0 8 329.5 9 286.5 10 273.0 11 281.0 12 312.5 13 353.0 14 430.0 15 520.5 16 594.0 17 690.5 18 757.5 19 799.5 20 839.0 21 868.5 22 912.0 23 1003.5 24 1110.0 25 1247.5 26 1413.0 27 1549.0 28 1746.5 29 1942.0 30 2032.0 31 2166.0 32 2279.0 33 2362.0 34 2397.5 35 2475.5 36 2630.0 37 2697.0 38 2839.5 39 2965.0 40 3035.0 41 3059.0 42 3027.0 43 3062.0 44 3277.0 45 3889.5 46 5189.0 47 7466.5 48 10937.0 49 16903.5 50 24299.0 51 23417.5 52 14687.0 53 8374.5 54 5526.0 55 4081.5 56 3230.5 57 2594.5 58 2040.5 59 1682.5 60 1423.5 61 1213.0 62 1044.5 63 878.5 64 724.0 65 609.5 66 512.5 67 418.5 68 333.0 69 245.0 70 176.0 71 124.0 72 82.0 73 64.5 74 46.5 75 26.5 76 19.5 77 12.5 78 11.5 79 11.0 80 7.0 81 5.0 82 1.5 83 1.5 84 1.5 85 2.5 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.59561919246249 2 25.660274125044047 3 6.445091529573233 4 24.975888866220302 5 13.2305209859599 6 10.492052599365692 7 8.412930986516312 8 8.982788359886493 9 5.636904872303726 10-11 7.454281580948495 12-13 10.566704378952835 14-15 7.7834912921713 16-17 8.48735092827865 18-19 7.102119925070014 20-21 8.673516701596899 22-23 6.3623254261179225 24-25 5.835589888161433 26-27 10.21616558784799 28-29 6.722369568039765 30-31 4.6849787636552485 32-33 9.46014244115956 34-35 7.972670957212011 36-37 7.369428937069941 38-39 6.826696589201922 40-41 9.33332715099134 42-43 7.039060036723112 44-45 7.026772632008457 46-47 6.430717584435336 48-49 4.2551514364671625 50-51 6.691071461691118 52-53 4.916352912810431 54-55 5.404139696199715 56-57 5.417818127863197 58-59 3.714969304671996 60-61 4.521996772817478 62-63 3.4152029971993993 64-65 4.802520540831277 66-67 2.724789954930727 68-69 5.373305265500677 70-71 2.6842183355898883 72-73 1.604781423298774 74-75 0.9931932414637313 76-77 1.3314446278539236 78-79 1.9965873472188735 80-81 2.4917929410019104 82-83 0.8863160042287219 84-85 1.3233303039857558 86-87 0.664910881540145 88-89 0.8654505999962906 90-91 0.43770981323144836 92-93 0.40223862603631505 94-95 0.5740304542166664 96-97 0.5673071572973274 98-99 0.4954374316078417 100 0.5656842925236938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 215668.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.5412946177608 #Duplication Level Percentage of deduplicated Percentage of total 1 90.82472130374964 50.44522604507574 2 4.584488217691161 5.0925682154087575 3 1.4981749647903084 2.496317313251158 4 0.7457380254471284 1.6567702151610464 5 0.45998811410509854 1.2774167683089723 6 0.3080711245482256 1.0266401455054728 7 0.23125453898610107 0.8990923537065043 8 0.1824022989215273 0.8104687858685936 9 0.13156708544662402 0.6576665629311947 >10 0.8661460889779012 9.170199355272475 >50 0.09269455995400461 3.6276674232187056 >100 0.05382264771522849 6.643065630798789 >500 0.012957304079592042 5.021604028081319 >1k 0.007973725587441258 11.175297157411263 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4917 2.27989316913033 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3804 1.7638221711148618 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3741 1.7346106051894579 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1926 0.893039301148061 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1777 0.8239516293562328 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1524 0.7066416900050076 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1253 0.5809855889608102 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1245 0.5772761837639334 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 967 0.44837435317246876 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 962 0.44605597492442084 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 951 0.44095554277871546 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 864 0.40061576126268156 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 666 0.30880798263998366 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 657 0.3046349017934974 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 647 0.29999814529740154 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 639 0.2962887401005249 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 578 0.26800452547434017 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 560 0.25965836378136764 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 552 0.25594895858449096 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 524 0.2429660403954226 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 504 0.2336925274032309 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 490 0.22720106830869669 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 490 0.22720106830869669 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 484 0.2244190144110392 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 483 0.22395533876142962 No Hit ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 447 0.20726301537548456 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 417 0.193352745887197 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 411 0.19057069198953946 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 396 0.1836155572453957 No Hit CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 356 0.16506853126101229 No Hit AAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 340 0.15764972086725892 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 315 0.1460578296270193 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 312 0.14466680267819057 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAA 302 0.14003004618209472 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 299 0.13863901923326594 No Hit GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 278 0.12890183059146465 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 275 0.1275108036426359 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 269 0.1247287497449784 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 263 0.12194669584732087 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 252 0.11684626370161545 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 236 0.10942745330786209 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.636756496095851E-4 0.0 2 0.0 0.0 0.0 4.636756496095851E-4 0.0 3 0.0 0.0 0.0 4.636756496095851E-4 0.0 4 0.0 0.0 0.0 4.636756496095851E-4 0.0 5 0.0 0.0 0.0 4.636756496095851E-4 0.0 6 0.0 0.0 0.0 4.636756496095851E-4 0.0 7 0.0 0.0 0.0 4.636756496095851E-4 0.0 8 0.0 0.0 0.0 4.636756496095851E-4 0.0 9 0.0 0.0 0.0 4.636756496095851E-4 0.0 10-11 0.0 0.0 0.0 4.636756496095851E-4 0.0 12-13 0.0 0.0 0.0 4.636756496095851E-4 0.0 14-15 0.0 0.0 0.0 4.636756496095851E-4 0.0 16-17 0.0 0.0 0.0 9.273512992191703E-4 0.0 18-19 0.0 0.0 0.0 9.273512992191703E-4 0.0 20-21 0.0 0.0 0.0 0.0011591891240239627 0.0 22-23 0.0 0.0 0.0 0.0018547025984383403 0.0 24-25 0.0 0.0 0.0 0.003477567372071888 0.0 26-27 0.0 0.0 0.0 0.004636756496095851 0.0 28-29 0.0 0.0 0.0 0.010200864291410872 0.0 30-31 0.0 0.0 0.0 0.03755772761837639 0.0 32-33 0.0 0.0 0.0 0.08508448170335886 0.0 34-35 0.0 0.0 0.0 0.16831426080827938 0.0 36-37 0.0 0.0 0.0 0.2675408498247306 0.0 38-39 0.0 0.0 0.0 0.43075467848730453 0.0 40-41 0.0 0.0 0.0 0.7544002819147949 0.0 42-43 0.0 0.0 0.0 0.9407978930578482 0.0 44-45 0.0 0.0 0.0 1.1227905855296103 0.0 46-47 0.0 0.0 0.0 1.3657566259250329 0.0 48-49 0.0 0.0 0.0 1.5435762375503088 0.0 50-51 0.0 0.0 0.0 1.7473616855537215 0.0 52-53 0.0 0.0 0.0 2.050837398223195 0.0 54-55 0.0 0.0 0.0 2.315828031975073 0.0 56-57 0.0 0.0 0.0 2.5611124506185434 0.0 58-59 0.0 0.0 0.0 2.8592558933175063 0.0 60-61 0.0 0.0 0.0 3.0994398798152716 0.0 62-63 0.0 0.0 0.0 3.333827920692917 0.0 64-65 0.0 0.0 0.0 3.681584657900106 0.0 66-67 0.0 0.0 0.0 4.016358476918226 0.0 68-69 0.0 0.0 0.0 4.49834931468739 0.0 70-71 0.0 0.0 0.0 5.351048834319417 0.0 72-73 0.0 0.0 0.0 5.938294044549957 0.0 74-75 0.0 0.0 0.0 6.450423799543743 0.0 76-77 0.0 0.0 0.0 7.064098521802029 0.0 78-79 0.0 0.0 0.0 7.607990058794073 0.0 80-81 0.0 0.0 0.0 8.198944674221488 0.0 82-83 0.0 0.0 0.0 9.111690190477958 0.0 84-85 0.0 0.0 0.0 9.916862956025001 0.0 86-87 0.0 0.0 0.0 10.593365728805386 0.0 88 0.0 0.0 0.0 11.237179368288295 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAT 20 0.0016722821 75.19788 6 GACTATG 25 0.004149504 59.7922 7 ATGCTAT 35 0.0064510955 53.478355 1 GTACAAA 85 4.2327883E-8 51.381157 1 ATGTTGT 30 0.008332268 50.131924 6 TACAGCA 35 0.009608748 48.353275 4 GAACAAA 230 0.0 46.11539 1 CTGTGCG 55 3.5623867E-5 45.650707 9 TGTGCGT 35 2.9296752E-6 43.0541 10-11 ACTGTGC 60 6.002746E-5 41.788235 8 TACTGTG 50 0.0012745203 39.861465 7 TACATAA 80 9.016595E-5 38.994633 2 AAAAGTA 115 4.5538764E-7 37.977383 2 ATCGGAA 95 5.7057514E-7 36.908493 5 TCGGAAG 100 8.4693056E-7 35.092346 6 AGAACAA 125 8.7482476E-7 34.93919 2 GTACATC 90 1.8030926E-4 34.661892 1 ACATGAA 150 6.162736E-9 33.85789 3 CATGAAA 150 6.182745E-9 33.84729 4 CGGAAGC 110 1.8708379E-6 31.707985 7 >>END_MODULE