FastQCFastQC Report
Wed 25 May 2016
SRR1295079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768780
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA1300.005743537619.162415
GTACATG75950.018.4291551
ATACCGT2502.8182403E-617.9868986
TACATGG77800.017.6732
GTATTAT19050.017.079661
CGCAGCG3008.579227E-716.6690048
TATACCG2404.088723E-516.6074265
GTATAGA12200.016.1023221
GTCTAAG8400.016.078361
GTACTAG6151.8189894E-1115.9714091
ACATGGG79950.015.8425373
GTCCTAG8150.015.8183071
GTATATA28450.015.5363341
TATACTG18500.015.3506455
GTGTTAG11650.015.2816561
GTATTAG11800.015.0873981
CTAGGAC9350.014.9192083
CTATACG2656.899651E-414.7390514
GTATCAA55350.014.6404561
TCAACGC56250.014.1850494
Produced by FastQC (version 0.11.5)