##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2768780 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.59030403282312 33.0 27.0 33.0 15.0 33.0 2 22.942424461315092 27.0 15.0 33.0 2.0 33.0 3 25.17883110973064 33.0 15.0 33.0 15.0 33.0 4 25.525667261393103 33.0 15.0 37.0 2.0 37.0 5 27.211910299843254 33.0 15.0 37.0 2.0 37.0 6 28.016482349626912 33.0 15.0 37.0 6.0 37.0 7 27.9040440193876 33.0 15.0 37.0 6.0 37.0 8 28.535544897030462 33.0 22.0 37.0 6.0 37.0 9 29.608614985661553 33.0 27.0 37.0 15.0 37.0 10-11 29.161587594536222 35.0 24.5 37.0 10.5 37.0 12-13 27.98303151568561 33.0 18.5 37.0 4.0 37.0 14-15 29.735507696530604 33.0 27.0 37.0 10.5 40.0 16-17 30.195008090205796 35.0 27.0 38.5 10.5 40.0 18-19 30.31888521298189 37.0 27.0 40.0 10.5 40.0 20-21 29.882165069091805 33.0 27.0 40.0 6.0 40.0 22-23 30.580096829650607 35.0 27.0 40.0 15.0 40.0 24-25 30.7020568625893 37.0 27.0 40.0 15.0 40.0 26-27 29.326298044626153 33.0 24.5 38.5 6.0 40.0 28-29 30.01536633463114 33.0 27.0 40.0 10.5 40.0 30-31 30.65197939164542 33.0 27.0 40.0 15.0 40.0 32-33 29.52276255245993 33.0 27.0 37.0 6.0 40.0 34-35 29.11251435650359 33.0 22.0 37.0 6.0 40.0 36-37 29.416832142676558 33.0 24.5 37.0 6.0 40.0 38-39 29.528343349778602 33.0 24.5 37.0 6.0 40.0 40-41 28.609325768027794 33.0 22.0 37.0 6.0 40.0 42-43 29.253351114931487 33.0 22.0 37.0 6.0 40.0 44-45 29.139701782012295 33.0 22.0 37.0 6.0 40.0 46-47 28.826923590895632 33.0 22.0 37.0 6.0 40.0 48-49 28.705499172920923 33.0 22.0 37.0 6.0 40.0 50-51 28.698077673199027 33.0 22.0 37.0 6.0 40.0 52-53 28.901660839792257 33.0 22.0 37.0 6.0 40.0 54-55 28.89940154147314 33.0 22.0 37.0 6.0 40.0 56-57 28.86945261089722 33.0 22.0 37.0 6.0 40.0 58-59 28.713347214296547 33.0 22.0 37.0 6.0 40.0 60-61 28.69896633174178 33.0 22.0 37.0 10.5 40.0 62-63 28.596233178511838 33.0 22.0 37.0 10.5 40.0 64-65 28.211260374605423 33.0 22.0 37.0 6.0 40.0 66-67 28.40950833941303 33.0 22.0 37.0 10.5 40.0 68-69 28.033234673755228 33.0 22.0 37.0 6.0 40.0 70-71 28.135382189989816 33.0 22.0 37.0 10.5 37.0 72-73 28.148402184355565 33.0 22.0 37.0 10.5 37.0 74-75 28.227929810241335 33.0 22.0 37.0 15.0 37.0 76-77 28.007411567549607 33.0 22.0 37.0 15.0 37.0 78-79 27.590624751695692 33.0 22.0 37.0 10.5 37.0 80-81 27.405157867363965 33.0 22.0 37.0 10.5 37.0 82-83 27.467876104276975 30.0 22.0 33.0 15.0 37.0 84-85 27.412530970319057 30.0 22.0 33.0 15.0 37.0 86-87 27.160024631787287 27.0 22.0 33.0 15.0 37.0 88-89 27.10247545850519 27.0 22.0 33.0 15.0 37.0 90-91 27.21320112107137 27.0 22.0 33.0 15.0 37.0 92-93 27.137455124639736 27.0 22.0 33.0 15.0 37.0 94-95 27.01841533093998 27.0 22.0 33.0 15.0 37.0 96-97 27.099530479127992 27.0 22.0 33.0 15.0 37.0 98-99 27.16172213032455 27.0 22.0 33.0 15.0 37.0 100 26.823506020702258 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.308714581597265 1101 2 11.234979242638143 1101 3 8.399188505389379 1101 4 12.902912634741991 1101 5 10.900851579027812 1101 6 9.947281324045125 1101 7 9.498555349804324 1101 8 8.882443685389795 1101 9 7.295478328719003 1101 10-11 7.676957223389259 1101 12-13 9.083391690528504 1101 14-15 7.882388407094112 1101 16-17 8.121619744206019 1101 18-19 8.072957256680478 1101 20-21 7.213134944748365 1101 22-23 7.306286582261752 1101 24-25 7.35439608556818 1101 26-27 8.586077126041424 1101 28-29 8.269799619501036 1101 30-31 5.477161890788697 1101 32-33 7.043960825001005 1101 34-35 8.784030830000287 1101 36-37 8.139488925168635 1101 38-39 8.273724580448352 1101 40-41 9.122347721609717 1101 42-43 8.69337474436864 1101 44-45 8.585355423609993 1101 46-47 7.465883726408187 1101 48-49 5.435977903154345 1101 50-51 6.888924374920389 1101 52-53 6.057450183790117 1101 54-55 4.186201130176695 1101 56-57 6.231378657518988 1101 58-59 6.220345245320903 1101 60-61 6.823377368056889 1101 62-63 6.287857868432248 1101 64-65 6.816163254656061 1101 66-67 5.918963084319142 1101 68-69 6.856363664067857 1101 70-71 6.265641919974371 1101 72-73 5.622686351189667 1101 74-75 5.020972128290428 1101 76-77 5.034753393068584 1101 78-79 5.143076638154184 1101 80-81 4.979111566665473 1101 82-83 4.5898909542525175 1101 84-85 4.372600208353457 1101 86-87 4.158387262315106 1101 88-89 4.000858334346201 1101 90-91 3.8064152673367033 1101 92-93 3.829870366276868 1101 94-95 3.8197993355491313 1101 96-97 3.7941326077059685 1101 98-99 3.5140147849726695 1101 100 3.714447859999769 1102 1 6.695189592640411 1102 2 10.46666199850516 1102 3 7.957783926844684 1102 4 12.58090344181149 1102 5 10.507325851241045 1102 6 9.424902323163241 1102 7 6.474192433406856 1102 8 7.6153035387870744 1102 9 6.337973781771073 1102 10-11 7.291941204927937 1102 12-13 8.915066193508192 1102 14-15 6.125901480453635 1102 16-17 7.169438977677846 1102 18-19 7.305374401621616 1102 20-21 6.895291730743924 1102 22-23 6.43322455670452 1102 24-25 6.802185608645605 1102 26-27 8.26889072397018 1102 28-29 7.495862276993634 1102 30-31 4.157730624764344 1102 32-33 4.22631025696656 1102 34-35 5.077944993684124 1102 36-37 5.136351884795509 1102 38-39 4.513740473151387 1102 40-41 6.111974431725869 1102 42-43 6.907286007752077 1102 44-45 6.381216364816424 1102 46-47 5.419822240039597 1102 48-49 3.5849561957026985 1102 50-51 5.001734624534862 1102 52-53 5.717679794835462 1102 54-55 5.2230487001336705 1102 56-57 6.313574782277499 1102 58-59 6.026817120266859 1102 60-61 6.095155322944251 1102 62-63 4.34721554228129 1102 64-65 6.10388197582008 1102 66-67 3.717846979556203 1102 68-69 6.034032490633038 1102 70-71 5.229306893620155 1102 72-73 4.960645192075571 1102 74-75 4.12473340134153 1102 76-77 3.1819713054496397 1102 78-79 4.275303809488843 1102 80-81 4.478492835912384 1102 82-83 3.8676574006849904 1102 84-85 2.9577709095030436 1102 86-87 3.0963616657885495 1102 88-89 3.3140614606971184 1102 90-91 3.3472601036230003 1102 92-93 3.1074014089230744 1102 94-95 3.07614050047955 1102 96-97 3.303962857603402 1102 98-99 3.0347436799817977 1102 100 3.205900698285621 1103 1 6.711580466478093 1103 2 10.99402051309772 1103 3 7.98381608904706 1103 4 12.230537706339373 1103 5 10.479793885411208 1103 6 9.2135164692242 1103 7 8.177856088134337 1103 8 8.22891543963302 1103 9 7.184452279313401 1103 10-11 7.49193463683627 1103 12-13 8.836988942026682 1103 14-15 8.566893476764783 1103 16-17 8.05923397279199 1103 18-19 7.272047904465328 1103 20-21 8.432013931295916 1103 22-23 6.976118350662151 1103 24-25 5.377376384900213 1103 26-27 6.155315617841886 1103 28-29 7.151720612639203 1103 30-31 6.089618709843915 1103 32-33 7.833605932347343 1103 34-35 7.1304965632569655 1103 36-37 4.1741532630224185 1103 38-39 6.422668185270588 1103 40-41 6.897302119181216 1103 42-43 7.5969729644603134 1103 44-45 5.875858678607674 1103 46-47 6.5704912470915815 1103 48-49 5.711353691249126 1103 50-51 6.593691661172752 1103 52-53 6.188166141917556 1103 54-55 4.97166604981485 1103 56-57 6.031676643996377 1103 58-59 5.660801431910745 1103 60-61 5.225045233685222 1103 62-63 2.963895546195335 1103 64-65 5.22598125855766 1103 66-67 4.182962035096793 1103 68-69 5.306199867055735 1103 70-71 3.8422068267328555 1103 72-73 4.5345904436804005 1103 74-75 3.4017460187796793 1103 76-77 3.5996772720917782 1103 78-79 3.0591276720361087 1103 80-81 4.3846240154478195 1103 82-83 3.5720903263945125 1103 84-85 3.3962815075881103 1103 86-87 2.8834798284386274 1103 88-89 3.043149796168432 1103 90-91 2.6674491440248076 1103 92-93 2.3927258793539288 1103 94-95 2.873397450927989 1103 96-97 3.077191986151675 1103 98-99 2.8107170644658197 1103 100 3.0142423087548913 1104 1 6.3684900573561904 1104 2 9.704534149138333 1104 3 7.195967145770364 1104 4 12.165098327630506 1104 5 9.294818131540755 1104 6 8.232701371797557 1104 7 4.034937211088987 1104 8 6.312188566762593 1104 9 6.468373615419171 1104 10-11 7.082158284936206 1104 12-13 7.31919918870847 1104 14-15 7.161773607741075 1104 16-17 7.046188502687233 1104 18-19 6.806281519002052 1104 20-21 7.506181428408571 1104 22-23 6.39284481667281 1104 24-25 5.84867183181397 1104 26-27 8.20748922544572 1104 28-29 7.098251916158212 1104 30-31 6.230340164427243 1104 32-33 7.577124412189068 1104 34-35 7.952128076335885 1104 36-37 6.593126300154616 1104 38-39 7.187723542610094 1104 40-41 7.114874528013438 1104 42-43 7.267607562621194 1104 44-45 6.701442346063292 1104 46-47 6.699579550307348 1104 48-49 5.418748123346134 1104 50-51 5.675719309192402 1104 52-53 5.975960132002115 1104 54-55 4.904099662507139 1104 56-57 5.957301377164182 1104 58-59 4.054386419761915 1104 60-61 4.517493997835128 1104 62-63 4.030346052789987 1104 64-65 2.845002630629832 1104 66-67 3.2057468057390928 1104 68-69 4.09147599192805 1104 70-71 2.604112330026517 1104 72-73 2.5195384768389246 1104 74-75 3.247476405291028 1104 76-77 3.903183589538955 1104 78-79 4.426807627465976 1104 80-81 3.0502605855223592 1104 82-83 2.9366979210016133 1104 84-85 2.996492071078034 1104 86-87 0.7636228477829121 1104 88-89 0.3892085998813819 1104 90-91 2.115880997997955 1104 92-93 2.3288098107259074 1104 94-95 2.3824368984232756 1104 96-97 2.8780752786995762 1104 98-99 2.5610773252249537 1104 100 0.7472108064170762 1105 1 5.642794534745274 1105 2 -2.8170622739897766 1105 3 0.3696426940480251 1105 4 8.507243642337318 1105 5 8.546681515183387 1105 6 8.060971392723882 1105 7 5.336577102633889 1105 8 6.016766130264401 1105 9 4.840279706797578 1105 10-11 4.899744101501504 1105 12-13 5.082320800605654 1105 14-15 5.704452614513038 1105 16-17 5.969818407391049 1105 18-19 6.0934903096098445 1105 20-21 6.905063889247462 1105 22-23 1.8892017812690796 1105 24-25 3.7628555242344923 1105 26-27 6.8234569183137594 1105 28-29 5.8818887207054615 1105 30-31 5.215787419635678 1105 32-33 6.980273468827956 1105 34-35 6.1136582154998145 1105 36-37 6.0903825177588615 1105 38-39 4.065637454136596 1105 40-41 5.2110547617769 1105 42-43 5.477835990464442 1105 44-45 6.079237584297783 1105 46-47 5.3255138600216085 1105 48-49 4.840469529837737 1105 50-51 5.751151222191762 1105 52-53 5.440963026876268 1105 54-55 5.167760044910491 1105 56-57 4.9776412252439215 1105 58-59 1.8934099039164458 1105 60-61 4.253741324532378 1105 62-63 -1.0839136344854694 1105 64-65 -0.5992901809942452 1105 66-67 0.6824536895713287 1105 68-69 2.6926925227093435 1105 70-71 2.7784728574530035 1105 72-73 2.4793789275223546 1105 74-75 2.5729798695094246 1105 76-77 1.6475216025435628 1105 78-79 3.0907615014402303 1105 80-81 1.5209661057714783 1105 82-83 0.7575523328945408 1105 84-85 1.1757509067922562 1105 86-87 -0.4825164519011551 1105 88-89 1.4229800699267443 1105 90-91 1.3345221808015921 1105 92-93 1.4635916219638325 1105 94-95 0.804376213982902 1105 96-97 1.9193080827374587 1105 98-99 1.7373722449446234 1105 100 1.8737307509904468 1106 1 6.0262961995378745 1106 2 10.260346899451168 1106 3 6.77043018010723 1106 4 11.728582136285791 1106 5 9.742791553417124 1106 6 9.314306364148994 1106 7 6.311381755762547 1106 8 7.254619229438617 1106 9 6.785836936010231 1106 10-11 5.575572102294295 1106 12-13 7.8443796839592 1106 14-15 7.650345198209926 1106 16-17 7.4303768671848225 1106 18-19 7.0496622098661526 1106 20-21 8.038760323384782 1106 22-23 6.100809401014498 1106 24-25 4.768218633668962 1106 26-27 7.517072189099693 1106 28-29 6.147274251319885 1106 30-31 5.266455594462922 1106 32-33 7.2041890864598805 1106 34-35 6.657248046942268 1106 36-37 5.849731144462261 1106 38-39 6.981690209778392 1106 40-41 6.464001683605606 1106 42-43 6.104468462367741 1106 44-45 4.382247076206092 1106 46-47 5.906403110967297 1106 48-49 5.758463660342766 1106 50-51 6.427290108048059 1106 52-53 5.594988881126685 1106 54-55 5.249234308191102 1106 56-57 4.849834100408636 1106 58-59 4.821192580795408 1106 60-61 4.679518233335678 1106 62-63 4.8507015512745895 1106 64-65 5.095879796829539 1106 66-67 2.8662189855417175 1106 68-69 3.2936204047529998 1106 70-71 2.736214798380871 1106 72-73 2.7279055772838348 1106 74-75 2.682537421332075 1106 76-77 3.4723258760205695 1106 78-79 4.159071873719125 1106 80-81 3.4323579062993375 1106 82-83 2.027161065515653 1106 84-85 2.5952664342629497 1106 86-87 3.1338746015312857 1106 88-89 3.1978700521110213 1106 90-91 3.1405425115095724 1106 92-93 2.768855922696879 1106 94-95 1.307448159258115 1106 96-97 1.4649784320430044 1106 98-99 1.948961365056718 1106 100 2.8368179590668383 1107 1 6.018816131536411 1107 2 10.39760249261931 1107 3 6.15138820068848 1107 4 11.50513237951348 1107 5 9.398193746899363 1107 6 8.081282355890579 1107 7 3.768909714651084 1107 8 6.319904655963043 1107 9 5.610279947490415 1107 10-11 4.742048729436661 1107 12-13 6.264844523825442 1107 14-15 6.221114984649169 1107 16-17 6.956358136902399 1107 18-19 6.311349633794453 1107 20-21 5.512851410728757 1107 22-23 5.872677632534849 1107 24-25 4.5318045444022665 1107 26-27 7.14503119487113 1107 28-29 6.547013904941672 1107 30-31 5.069315236049551 1107 32-33 4.985670815129513 1107 34-35 6.767004262533813 1107 36-37 6.453255505890745 1107 38-39 6.5042762729686245 1107 40-41 7.686400518128007 1107 42-43 6.125056486450699 1107 44-45 4.367340717258141 1107 46-47 3.4993748056700085 1107 48-49 4.001827489969198 1107 50-51 4.938951958907886 1107 52-53 3.85964729309039 1107 54-55 5.263905765691334 1107 56-57 5.219046476072542 1107 58-59 3.1371828752433935 1107 60-61 2.8397879250618843 1107 62-63 0.3854697464037571 1107 64-65 2.073926040025821 1107 66-67 4.088677717752212 1107 68-69 4.909359559672684 1107 70-71 4.2708694687156665 1107 72-73 2.3930999386021767 1107 74-75 2.542984246089379 1107 76-77 3.162890752387053 1107 78-79 3.90620090146378 1107 80-81 2.244247322026151 1107 82-83 1.6644497700821503 1107 84-85 2.3930045372516418 1107 86-87 2.4336486397521746 1107 88-89 2.5724325563436636 1107 90-91 1.454644720139349 1107 92-93 2.2155044084931426 1107 94-95 2.2764626244543678 1107 96-97 2.405576706073532 1107 98-99 1.875467024447655 1107 100 0.03624157263847039 1108 1 4.3069230279732515 1108 2 -3.2271627678034918 1108 3 0.08873578283372296 1108 4 6.737626983760617 1108 5 7.01084251975524 1108 6 6.790615383769708 1108 7 4.302955076249816 1108 8 4.981817815266648 1108 9 4.847571683634719 1108 10-11 3.2281225658985946 1108 12-13 2.3107465225053296 1108 14-15 3.847686333770465 1108 16-17 4.63977575877318 1108 18-19 3.7915170052158835 1108 20-21 4.5952711440599785 1108 22-23 3.9587780725406425 1108 24-25 2.958459126495722 1108 26-27 4.613312158971688 1108 28-29 6.037313301191311 1108 30-31 4.479077351217665 1108 32-33 4.569191263903097 1108 34-35 3.0219091363096418 1108 36-37 1.1167952253280902 1108 38-39 2.303275778502261 1108 40-41 5.349984109497573 1108 42-43 3.0822384289613183 1108 44-45 3.6294634310109366 1108 46-47 4.7119646235553105 1108 48-49 4.737027268894245 1108 50-51 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2213 46-47 -17.252568336783515 2213 48-49 -6.283071416552875 2213 50-51 -23.826766603966366 2213 52-53 -18.15847655014699 2213 54-55 -18.040587982645732 2213 56-57 -17.948755847515386 2213 58-59 -18.431543529715807 2213 60-61 -19.73207711252004 2213 62-63 -17.97615187153912 2213 64-65 -17.981832024945064 2213 66-67 -8.895675948676903 2213 68-69 -23.27743352083297 2213 70-71 -17.82402306477556 2213 72-73 -9.704803864118734 2213 74-75 -4.488303033224373 2213 76-77 -3.2743720413954094 2213 78-79 -4.8305138489347605 2213 80-81 -3.2384118117277723 2213 82-83 -3.158853412479502 2213 84-85 -3.027675210575321 2213 86-87 -3.0382294501469396 2213 88-89 -2.7053116386564966 2213 90-91 -2.685824098676985 2213 92-93 -3.76063055165638 2213 94-95 -3.6412338060326057 2213 96-97 -3.3452588123683924 2213 98-99 -3.8427799934503035 2213 100 -3.3103444402683344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 753.0 3 577.0 4 2635.0 5 9731.0 6 16365.0 7 19688.0 8 17426.0 9 17654.0 10 21163.0 11 27383.0 12 35777.0 13 49852.0 14 40498.0 15 44641.0 16 52358.0 17 55844.0 18 55808.0 19 56733.0 20 56632.0 21 55168.0 22 57660.0 23 63234.0 24 68916.0 25 75898.0 26 82875.0 27 90208.0 28 97016.0 29 102251.0 30 107533.0 31 117279.0 32 131596.0 33 160055.0 34 193475.0 35 228711.0 36 254122.0 37 226483.0 38 74618.0 39 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.83238469528027 22.176529134472784 14.436912375729577 23.55417379451737 2 14.092576489706815 21.81088169582627 41.54233770207318 22.554204112393727 3 16.669384042098496 28.023162705012645 30.74608716984841 24.561366083040454 4 11.206706169031692 18.398918987711347 41.80538035686881 28.588994486388152 5 12.076327310741254 38.43600875534951 36.910740277903805 12.576923656005427 6 28.307408653916504 40.00177177133234 19.736108897495512 11.954710677255637 7 24.25048849683795 33.34981430701525 25.797223338459162 16.602473857687635 8 22.43586161294722 37.57536985893443 22.829080367551285 17.15968816056706 9 23.704166433339942 17.370291782005744 23.311534311136267 35.61400747351805 10-11 22.03429961051014 28.202153744704116 30.50076417438346 19.262782470402286 12-13 22.940738752539502 25.809620561933116 30.487701575667113 20.761939109860265 14-15 20.45848565481738 27.34579592704022 27.60908471891555 24.58663369922684 16-17 19.979155279219086 31.019145637712242 28.478523330373324 20.523175752695348 18-19 19.909208794812415 30.164172186879973 29.9760926220818 19.950526396225815 20-21 21.444485106270804 29.513243048572193 28.808738140336544 20.23353370482047 22-23 20.362710513851294 29.615031887358885 29.457585262372728 20.564672336417097 24-25 20.719060445338922 29.363863689670044 28.963372651680803 20.95370321331023 26-27 20.670501096045633 29.366745668963567 29.041560745251704 20.92119248973909 28-29 20.413050962937263 29.327715068853287 29.467439273564054 20.79179469464539 30-31 21.077297482353046 29.655758665063015 28.769882383678762 20.497061468905176 32-33 20.219275869677826 29.48997732397915 29.47348673945737 20.817260066885655 34-35 20.34878469082358 28.985100263464492 29.268326036489807 21.397789009222123 36-37 20.888611997647917 29.633022779003433 28.857159168913633 20.621206054435014 38-39 20.571074919187062 29.313211427592083 29.1266870742146 20.98902657900625 40-41 20.823108742901216 29.378310399328804 28.715278462672085 21.0833023950979 42-43 20.461117615114656 29.45799779456021 29.300373602952455 20.780510987372683 44-45 20.901199321004267 28.99297261955629 29.124844638754904 20.980983420684538 46-47 20.775369826748122 29.46250127680603 29.21022152418209 20.55190737226376 48-49 20.637579282458354 29.180299800916522 29.30594426148552 20.8761766551396 50-51 20.975666123205485 29.222172143053697 28.96843555999114 20.833726173749675 52-53 20.394044860549545 29.238533917464842 29.315177725451935 21.052243496533674 54-55 20.862785537716466 29.19469029549473 28.938262553223076 21.004261613565728 56-57 20.9647681228277 29.242403442495977 28.8940072119771 20.89882122269922 58-59 20.595045449167458 28.83281768069037 29.30669640403318 21.265440466109 60-61 21.05365257596267 29.27594239205994 28.730807766760723 20.939597265216666 62-63 20.564477776968655 29.331566557633348 29.223728345046613 20.880227320351384 64-65 20.63287592090329 28.793602829087078 29.270943613532513 21.30257763647712 66-67 20.73997243050643 29.151892578845857 28.925819783368862 21.182315207278847 68-69 21.12631108853512 29.02990070236296 28.887671967513235 20.95611624158869 70-71 21.24721695527257 29.12153301477249 28.626456132873862 21.004793897081083 72-73 20.794879275827014 29.245022280809586 29.178379359839923 20.781719083523477 74-75 21.092109101848326 28.78645960666841 28.810416861690474 21.31101442979279 76-77 21.092518217507692 29.253294535422114 28.778048071836892 20.876139175233302 78-79 21.035523468455295 29.003590013036607 28.996060438847763 20.96482607966033 80-81 21.22141865329144 28.93878398785299 28.747921828236546 21.091875530619024 82-83 20.67561040651837 29.165376779930085 29.24088855214993 20.918124261401612 84-85 20.90661017459444 28.781397029239574 28.817835875567816 21.49415692059817 86-87 21.127978030530834 29.10407276561954 28.83396763269762 20.933981571152003 88-89 20.8981674006441 28.698463026909117 29.095544998686147 21.30782457376064 90-91 21.174091554388298 28.706807078241443 28.791634996550926 21.32746637081934 92-93 20.745934424828064 29.026218042481794 29.117375167444393 21.11047236524575 94-95 20.756182973792157 28.57318305869172 29.012048481277468 21.658585486238653 96-97 20.941737056181417 28.80821319147369 29.002288154334043 21.24776159801085 98-99 20.889184165042607 28.606281645581095 29.02883922866197 21.475694960714332 100 21.09350790839376 28.470724935188535 28.70065023483622 21.735116921581483 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 357.5 2 348.0 3 284.0 4 323.0 5 477.0 6 739.0 7 1006.5 8 1393.0 9 2024.5 10 2841.5 11 3637.5 12 4398.0 13 5236.0 14 6064.5 15 6885.5 16 7728.0 17 8523.5 18 9221.5 19 10097.0 20 11292.0 21 12712.0 22 14572.5 23 17122.5 24 20654.5 25 25090.0 26 30980.0 27 38195.0 28 46629.0 29 55766.0 30 65131.0 31 74584.5 32 82931.0 33 90391.5 34 96840.0 35 102574.5 36 107372.0 37 110624.5 38 112750.0 39 113299.5 40 112445.5 41 109780.0 42 105433.5 43 100588.0 44 95144.5 45 89432.5 46 83702.0 47 78299.5 48 73754.0 49 69981.0 50 66787.0 51 62124.5 52 55757.0 53 49866.5 54 45014.5 55 40929.0 56 36847.5 57 33010.0 58 29457.5 59 25590.0 60 21656.5 61 17889.0 62 14367.5 63 11440.5 64 9125.0 65 7161.0 66 5503.5 67 4214.5 68 3210.5 69 2386.5 70 1759.5 71 1301.5 72 981.5 73 744.5 74 560.5 75 401.0 76 271.5 77 180.5 78 123.5 79 85.5 80 64.5 81 43.0 82 25.5 83 21.5 84 20.0 85 13.5 86 8.5 87 7.5 88 6.5 89 6.0 90 3.0 91 2.0 92 1.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 6.703421723647238 2 23.035669139476592 3 5.18993925122256 4 22.808746090335816 5 11.24361632199019 6 8.676637363748654 7 7.156184312224156 8 7.580920116441177 9 4.545250976964583 10-11 5.958924147097278 12-13 8.969853148318032 14-15 6.514331221693309 16-17 6.799763794884389 18-19 5.94352025079638 20-21 6.834706982858877 22-23 4.951440706737263 24-25 4.529070565375364 26-27 8.68153121591459 28-29 5.483967668070414 30-31 3.7873720555623778 32-33 7.684828697115698 34-35 6.240817255253217 36-37 6.056042733622751 38-39 5.168973338437867 40-41 7.532974812011066 42-43 5.459498407240734 44-45 5.4569341009397645 46-47 5.061814228649441 48-49 3.386744342273492 50-51 5.763477054876154 52-53 3.7509300124964784 54-55 4.139765528499916 56-57 4.10337766091925 58-59 3.080327797802642 60-61 3.4655696732857075 62-63 2.7899652554554715 64-65 3.915677663086269 66-67 1.9963124553052245 68-69 4.3677179118600975 70-71 2.0360230859801067 72-73 1.338387304155621 74-75 0.6518213798134919 76-77 0.9273759561973143 78-79 1.4281380246895745 80-81 1.6662392822831718 82-83 0.6578890341594492 84-85 1.0319527011896936 86-87 0.369278165834772 88-89 0.6260157903480955 90-91 0.2851978127550762 92-93 0.29478687364109823 94-95 0.4544239701240257 96-97 0.45610341016621037 98-99 0.4076163508837828 100 0.34755379625683513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2768780.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.1480775087762 #Duplication Level Percentage of deduplicated Percentage of total 1 97.9319822016189 95.13903797510963 2 1.8073392482820896 3.5115906675352346 3 0.17362455713004732 0.5060187580049034 4 0.040050465668565474 0.15563302972129517 5 0.017633793521052404 0.08565445698784772 6 0.006358047830268344 0.03706032740516492 7 0.006526478339655433 0.04438243765201364 8 0.003923395292677613 0.03049202479924899 9 0.0021032343568482535 0.018389265688639366 >10 0.00887860461107607 0.13559873860837823 >50 5.676955077435619E-4 0.03977941994041342 >100 9.377460111421316E-4 0.19513897514110773 >500 0.0 0.0 >1k 7.453182993970368E-5 0.10122392340610857 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.6116990154508485E-5 0.0 3.6116990154508485E-5 3.6116990154508485E-5 0.0 7 3.6116990154508485E-5 0.0 3.6116990154508485E-5 3.6116990154508485E-5 0.0 8 3.6116990154508485E-5 0.0 3.6116990154508485E-5 3.6116990154508485E-5 0.0 9 3.6116990154508485E-5 0.0 3.6116990154508485E-5 7.223398030901697E-5 0.0 10-11 3.6116990154508485E-5 0.0 3.6116990154508485E-5 7.223398030901697E-5 0.0 12-13 3.6116990154508485E-5 0.0 3.6116990154508485E-5 7.223398030901697E-5 0.0 14-15 3.6116990154508485E-5 0.0 3.6116990154508485E-5 7.223398030901697E-5 0.0 16-17 7.223398030901697E-5 1.8058495077254243E-5 3.6116990154508485E-5 7.223398030901697E-5 0.0 18-19 7.223398030901697E-5 3.6116990154508485E-5 3.6116990154508485E-5 7.223398030901697E-5 0.0 20-21 7.223398030901697E-5 3.6116990154508485E-5 3.6116990154508485E-5 1.4446796061803394E-4 0.0 22-23 9.029247538627121E-5 3.6116990154508485E-5 3.6116990154508485E-5 2.167019409270509E-4 0.0 24-25 1.0835097046352545E-4 3.6116990154508485E-5 3.6116990154508485E-5 2.167019409270509E-4 0.0 26-27 1.0835097046352545E-4 3.6116990154508485E-5 3.6116990154508485E-5 2.528189310815594E-4 0.0 28-29 1.0835097046352545E-4 3.6116990154508485E-5 3.6116990154508485E-5 6.681643178584069E-4 0.0 30-31 1.0835097046352545E-4 3.6116990154508485E-5 3.6116990154508485E-5 0.001968375963420712 0.0 32-33 1.0835097046352545E-4 3.6116990154508485E-5 5.417548523176273E-5 0.0045146237693135605 0.0 34-35 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.008126322784764408 0.0 36-37 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.014627381012575935 0.0 38-39 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.02690715766510882 0.0 40-41 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.04292504279863334 0.0 42-43 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.059376331814011946 0.0 44-45 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.0779043477632748 0.0 46-47 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.1023736085929543 3.6116990154508485E-5 48-49 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.12975028713007172 3.6116990154508485E-5 50-51 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.15866193774875575 3.6116990154508485E-5 52-53 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.18932526238993347 3.6116990154508485E-5 54-55 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.2232932916302487 3.6116990154508485E-5 56-57 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.266146100448573 3.6116990154508485E-5 58-59 1.0835097046352545E-4 3.6116990154508485E-5 7.223398030901697E-5 0.31463315973100064 3.6116990154508485E-5 60-61 1.4446796061803394E-4 3.6116990154508485E-5 7.223398030901697E-5 0.36400508527221376 3.6116990154508485E-5 62-63 1.4446796061803394E-4 3.6116990154508485E-5 7.223398030901697E-5 0.4176749326418133 3.6116990154508485E-5 64-65 1.4446796061803394E-4 3.6116990154508485E-5 7.223398030901697E-5 0.4769429134853618 3.6116990154508485E-5 66-67 1.4446796061803394E-4 3.6116990154508485E-5 7.223398030901697E-5 0.5479308576340483 3.6116990154508485E-5 68-69 1.4446796061803394E-4 3.6116990154508485E-5 7.223398030901697E-5 0.6318667427531259 3.6116990154508485E-5 70-71 1.8058495077254242E-4 3.6116990154508485E-5 7.223398030901697E-5 0.7289492122884447 3.6116990154508485E-5 72-73 1.8058495077254242E-4 5.417548523176273E-5 7.223398030901697E-5 0.8330744949038926 3.6116990154508485E-5 74-75 1.8058495077254242E-4 9.029247538627121E-5 7.223398030901697E-5 0.9411004124560276 3.6116990154508485E-5 76-77 1.8058495077254242E-4 1.0835097046352545E-4 7.223398030901697E-5 1.061315814185309 3.6116990154508485E-5 78-79 1.8058495077254242E-4 1.0835097046352545E-4 7.223398030901697E-5 1.2016844964208064 3.6116990154508485E-5 80-81 1.8058495077254242E-4 1.4446796061803394E-4 7.223398030901697E-5 1.360761779556339 3.6116990154508485E-5 82-83 1.8058495077254242E-4 1.8058495077254242E-4 7.223398030901697E-5 1.5347012041404517 3.6116990154508485E-5 84-85 1.8058495077254242E-4 1.8058495077254242E-4 7.223398030901697E-5 1.7089295646458007 3.6116990154508485E-5 86-87 2.167019409270509E-4 1.8058495077254242E-4 7.223398030901697E-5 1.8976227797080303 3.6116990154508485E-5 88 2.167019409270509E-4 1.8058495077254242E-4 7.223398030901697E-5 2.0597158315214643 3.6116990154508485E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCA 130 0.0057435376 19.16241 5 GTACATG 7595 0.0 18.429155 1 ATACCGT 250 2.8182403E-6 17.986898 6 TACATGG 7780 0.0 17.673 2 GTATTAT 1905 0.0 17.07966 1 CGCAGCG 300 8.579227E-7 16.669004 8 TATACCG 240 4.088723E-5 16.607426 5 GTATAGA 1220 0.0 16.102322 1 GTCTAAG 840 0.0 16.07836 1 GTACTAG 615 1.8189894E-11 15.971409 1 ACATGGG 7995 0.0 15.842537 3 GTCCTAG 815 0.0 15.818307 1 GTATATA 2845 0.0 15.536334 1 TATACTG 1850 0.0 15.350645 5 GTGTTAG 1165 0.0 15.281656 1 GTATTAG 1180 0.0 15.087398 1 CTAGGAC 935 0.0 14.919208 3 CTATACG 265 6.899651E-4 14.739051 4 GTATCAA 5535 0.0 14.640456 1 TCAACGC 5625 0.0 14.185049 4 >>END_MODULE