FastQCFastQC Report
Wed 25 May 2016
SRR1295079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768780
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40020.14454019459834297No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32300.11665787819906241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82100.020.823521
CAACGCA88450.019.3067135
AACGCAG88450.019.2211746
CTGTCGC4400.019.2102979
ATCAACG89350.019.0059073
TCAACGC89150.018.9440424
TCTGTCG4400.018.142738
TATCAAC93700.017.964632
TATACCG2152.6037336E-517.5045555
ACGCAGA97000.017.2823527
CGCAGAG97100.017.2161948
GAGTACT36450.017.19848312-13
ATACGCC1952.4520024E-416.886493
GTATTAA17900.016.5145231
GTTCGGG1850.002464622315.9789171
TACATGG101000.015.7402952
GTACATG100150.015.6438621
GCAGAGT108150.015.0232479
ACATGGG99200.014.8899753
AGTACTT45600.014.8802212-13
Produced by FastQC (version 0.11.5)