##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2768780 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43649188451231 33.0 33.0 33.0 27.0 33.0 2 30.409390778610074 33.0 33.0 33.0 27.0 33.0 3 31.081911166651015 33.0 33.0 33.0 27.0 33.0 4 35.2536452878163 37.0 37.0 37.0 33.0 37.0 5 35.51761967364688 37.0 37.0 37.0 33.0 37.0 6 35.262902794732696 37.0 37.0 37.0 33.0 37.0 7 35.17131949811831 37.0 37.0 37.0 33.0 37.0 8 35.156449772101794 37.0 37.0 37.0 33.0 37.0 9 35.137308850829605 37.0 37.0 37.0 33.0 37.0 10-11 35.109533621306134 37.0 37.0 37.0 33.0 37.0 12-13 35.03680971402567 37.0 37.0 37.0 33.0 37.0 14-15 36.5051775511236 40.0 37.0 40.0 33.0 40.0 16-17 36.492653804202575 40.0 37.0 40.0 33.0 40.0 18-19 36.524451563504506 40.0 37.0 40.0 33.0 40.0 20-21 36.53333778776212 40.0 37.0 40.0 33.0 40.0 22-23 36.49135160612255 40.0 37.0 40.0 33.0 40.0 24-25 36.46631639205715 40.0 37.0 40.0 33.0 40.0 26-27 36.445334407211845 40.0 37.0 40.0 33.0 40.0 28-29 36.39652680964179 40.0 37.0 40.0 33.0 40.0 30-31 36.306069099025564 37.0 37.0 40.0 33.0 40.0 32-33 36.269586424345746 37.0 37.0 40.0 30.0 40.0 34-35 36.17524288675879 37.0 37.0 40.0 27.0 40.0 36-37 36.166294541278106 37.0 37.0 40.0 27.0 40.0 38-39 36.10378307413373 37.0 37.0 40.0 27.0 40.0 40-41 36.04653240777527 37.0 37.0 40.0 27.0 40.0 42-43 35.96615693554562 37.0 37.0 40.0 27.0 40.0 44-45 35.87168427971886 37.0 37.0 40.0 27.0 40.0 46-47 35.77005955691676 37.0 37.0 40.0 27.0 40.0 48-49 35.665331481735635 37.0 37.0 40.0 27.0 40.0 50-51 35.573764076596916 37.0 37.0 40.0 27.0 40.0 52-53 35.48325616336437 37.0 37.0 40.0 27.0 40.0 54-55 35.37320986138299 37.0 35.0 40.0 27.0 40.0 56-57 35.26283218601695 37.0 33.0 40.0 27.0 40.0 58-59 35.14545846907302 37.0 33.0 40.0 27.0 40.0 60-61 35.000267988066945 37.0 33.0 40.0 27.0 40.0 62-63 34.833084607661135 37.0 33.0 40.0 27.0 40.0 64-65 34.662997060077004 37.0 33.0 40.0 27.0 40.0 66-67 34.44682856709453 37.0 33.0 40.0 27.0 40.0 68-69 34.238999306553794 37.0 33.0 40.0 27.0 40.0 70-71 34.019030403282315 37.0 33.0 37.0 27.0 40.0 72-73 33.80560156458802 37.0 33.0 37.0 27.0 40.0 74-75 33.572502148960915 37.0 33.0 37.0 24.5 40.0 76-77 33.333215170580544 37.0 33.0 37.0 22.0 40.0 78-79 33.091418964309185 37.0 33.0 37.0 22.0 37.0 80-81 32.86238216831962 37.0 33.0 37.0 22.0 37.0 82-83 32.64159196469203 37.0 33.0 37.0 22.0 37.0 84-85 32.46560669320061 37.0 33.0 37.0 22.0 37.0 86-87 32.479646992538235 37.0 33.0 37.0 22.0 37.0 88-89 32.47819996532769 37.0 33.0 37.0 22.0 37.0 90-91 32.47212057295993 37.0 33.0 37.0 22.0 37.0 92-93 32.431721191282804 35.0 33.0 37.0 22.0 37.0 94-95 32.44812968166485 35.0 33.0 37.0 22.0 37.0 96-97 32.394858565866556 33.0 33.0 37.0 22.0 37.0 98-99 32.31757994495771 33.0 33.0 37.0 22.0 37.0 100 31.78930359219584 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6450068324383054 1101 2 4.046967092171599 1101 3 2.3079193231184405 1101 4 1.1302833931134302 1101 5 0.5906617888361367 1101 6 0.5697074422062016 1101 7 0.5413132170020276 1101 8 0.494674175288047 1101 9 0.5901671844799665 1101 10-11 0.621069859210813 1101 12-13 0.6926046063171967 1101 14-15 0.9818887888553931 1101 16-17 0.9610359230521723 1101 18-19 0.9438220206349683 1101 20-21 0.9507498923445539 1101 22-23 0.9509934845104624 1101 24-25 0.9453495906325671 1101 26-27 0.9353794830595987 1101 28-29 0.7872232080263473 1101 30-31 0.774464992233419 1101 32-33 0.9901492930741611 1101 34-35 1.1196132188820798 1101 36-37 1.0043532355351061 1101 38-39 1.0140921115785275 1101 40-41 1.0046083485272845 1101 42-43 1.0030411791726763 1101 44-45 1.0095711601545503 1101 46-47 1.0666801114196645 1101 48-49 1.043345668530769 1101 50-51 1.0157229570250408 1101 52-53 1.0360974589823826 1101 54-55 1.0097439407912319 1101 56-57 1.0549004813117406 1101 58-59 1.0368420633147792 1101 60-61 0.9795141101832456 1101 62-63 0.8850142974502475 1101 64-65 0.997789018601388 1101 66-67 0.9526706242511978 1101 68-69 0.9583945443165831 1101 70-71 0.9475939498543724 1101 72-73 0.8992735861187953 1101 74-75 0.8477103239511052 1101 76-77 0.8561538017411721 1101 78-79 0.92811059420616 1101 80-81 0.8950879892662087 1101 82-83 0.9265888813786987 1101 84-85 0.9420366090758279 1101 86-87 0.9607923484543903 1101 88-89 0.8588450750957293 1101 90-91 0.9348729479747888 1101 92-93 0.9963194370683865 1101 94-95 0.9596823811672337 1101 96-97 0.8765546809132942 1101 98-99 0.9167646735773758 1101 100 1.0415709551810757 1102 1 0.5471124190928371 1102 2 4.050986877093493 1102 3 2.303144202508385 1102 4 1.1066403642880616 1102 5 0.4525885472120308 1102 6 0.5263280656214491 1102 7 0.48832700796975104 1102 8 0.505517054461059 1102 9 0.5830008487147822 1102 10-11 0.6434162022385408 1102 12-13 0.7108316188820183 1102 14-15 0.9017891332758907 1102 16-17 0.9013480944104089 1102 18-19 0.9219113121599563 1102 20-21 0.8983730214935974 1102 22-23 0.8731687962731058 1102 24-25 0.9860577040854039 1102 26-27 0.8799166218385821 1102 28-29 0.900222605958561 1102 30-31 0.9186314584728308 1102 32-33 1.0076204590578044 1102 34-35 1.0168283410012364 1102 36-37 1.0040613977192763 1102 38-39 0.9289052583494524 1102 40-41 0.8232243864811295 1102 42-43 0.9294634267561506 1102 44-45 1.0073198510894201 1102 46-47 0.910483843611118 1102 48-49 0.9036579263478188 1102 50-51 0.9314176300613681 1102 52-53 0.8855593707283589 1102 54-55 0.8454510403179043 1102 56-57 0.8029206136409002 1102 58-59 0.8515336175456838 1102 60-61 0.909885285755955 1102 62-63 0.9593230984864931 1102 64-65 0.8631584885773904 1102 66-67 0.892384697074668 1102 68-69 0.8524222048509671 1102 70-71 0.8508767126380974 1102 72-73 0.8202871664414033 1102 74-75 0.7572246454615836 1102 76-77 0.8067069023902391 1102 78-79 0.777190633065473 1102 80-81 0.8410672218577275 1102 82-83 0.8281087488294716 1102 84-85 0.8165438331032036 1102 86-87 0.7935605555028289 1102 88-89 0.7576734008914272 1102 90-91 0.7190194658065323 1102 92-93 0.827285960213409 1102 94-95 0.8108753949802079 1102 96-97 0.8388785996671473 1102 98-99 0.8632182506931372 1102 100 0.8576460863233883 1103 1 0.4703732487366459 1103 2 3.910882377414527 1103 3 2.2409613977723915 1103 4 1.0683837770953062 1103 5 0.35511498378102146 1103 6 0.3972089003320747 1103 7 0.3843270401400005 1103 8 0.43015275994783053 1103 9 0.4526202076958441 1103 10-11 0.48182090660068866 1103 12-13 0.4806159844110667 1103 14-15 0.7246939455424055 1103 16-17 0.6941404850751383 1103 18-19 0.7384854893724366 1103 20-21 0.7156128546908747 1103 22-23 0.6735710583774974 1103 24-25 0.6268461565759793 1103 26-27 0.6300288155677904 1103 28-29 0.6516679212336669 1103 30-31 0.6894430601354884 1103 32-33 0.7874592995349374 1103 34-35 0.8823750139947109 1103 36-37 0.7718337825129353 1103 38-39 0.6703472359616356 1103 40-41 0.6656830108546501 1103 42-43 0.7563253131925052 1103 44-45 0.7953503752901554 1103 46-47 0.815835497898938 1103 48-49 0.7350814194734454 1103 50-51 0.702395712741577 1103 52-53 0.617372190839049 1103 54-55 0.7292740315876998 1103 56-57 0.6060134475847931 1103 58-59 0.6048690017321334 1103 60-61 0.6294696595956495 1103 62-63 0.7331388648554551 1103 64-65 0.6871595689614409 1103 66-67 0.5991011740039127 1103 68-69 0.6781502912173494 1103 70-71 0.645619223338052 1103 72-73 0.6372523461725308 1103 74-75 0.6915884989743688 1103 76-77 0.6003887333061115 1103 78-79 0.7080887458784773 1103 80-81 0.6477192912757559 1103 82-83 0.6428281103573497 1103 84-85 0.6450845371758263 1103 86-87 0.6579856263108752 1103 88-89 0.6487080897984541 1103 90-91 0.6156438017925652 1103 92-93 0.6789521108157359 1103 94-95 0.6618732087439767 1103 96-97 0.6810479599216137 1103 98-99 0.664446148729553 1103 100 0.6763079113681876 1104 1 0.42779586265331204 1104 2 3.5543920148373793 1104 3 2.149200633483609 1104 4 0.8696861080762659 1104 5 0.30983105805083255 1104 6 0.3293504419616937 1104 7 0.30265336011513 1104 8 0.22778369691331335 1104 9 0.3513610038498811 1104 10-11 0.3740146510504445 1104 12-13 0.419676311949452 1104 14-15 0.5375751256744152 1104 16-17 0.4901194900263519 1104 18-19 0.5057514243063324 1104 20-21 0.5579583035846767 1104 22-23 0.5559992462729326 1104 24-25 0.47016646858860156 1104 26-27 0.4864737725952679 1104 28-29 0.5123209745534538 1104 30-31 0.43739907805250766 1104 32-33 0.6074750368706177 1104 34-35 0.7045876313127266 1104 36-37 0.5779149526633134 1104 38-39 0.5318736273271014 1104 40-41 0.48467696057871734 1104 42-43 0.5756863317957652 1104 44-45 0.5838686842507883 1104 46-47 0.596857686945512 1104 48-49 0.4893117031848959 1104 50-51 0.40652528509941277 1104 52-53 0.4360824236876084 1104 54-55 0.4354824674723048 1104 56-57 0.4652108922884892 1104 58-59 0.4362892352479406 1104 60-61 0.49895067470533405 1104 62-63 0.5721498267424536 1104 64-65 0.5491409651631116 1104 66-67 0.4620839063330422 1104 68-69 0.4838573673766149 1104 70-71 0.5093702967697311 1104 72-73 0.5335233408144617 1104 74-75 0.3575102506195833 1104 76-77 0.4267561211633577 1104 78-79 0.4862754749809355 1104 80-81 0.38754603411380373 1104 82-83 0.3838957483519678 1104 84-85 0.4974605439503179 1104 86-87 0.5097607554617269 1104 88-89 0.4442574779627435 1104 90-91 0.40168094429167667 1104 92-93 0.47369654049870036 1104 94-95 0.46054852759997544 1104 96-97 0.5256207219081119 1104 98-99 0.49030892198334897 1104 100 0.4431718959791269 1105 1 0.5068274191417714 1105 2 3.717211988497848 1105 3 2.1703975091789474 1105 4 1.003023045872908 1105 5 0.34637627383655456 1105 6 0.3889349603776324 1105 7 0.2968141838165579 1105 8 0.32971403079422856 1105 9 0.4201099380419109 1105 10-11 0.3439763912911147 1105 12-13 0.40741141569099426 1105 14-15 0.5852985001704312 1105 16-17 0.5603052405176641 1105 18-19 0.5696301823866108 1105 20-21 0.5031312969344697 1105 22-23 0.3858658427891086 1105 24-25 0.5124734768426435 1105 26-27 0.4550104838370075 1105 28-29 0.49628819788272693 1105 30-31 0.4707066113500744 1105 32-33 0.5568514710486809 1105 34-35 0.6838429321932651 1105 36-37 0.5897499583464523 1105 38-39 0.5151662481298374 1105 40-41 0.4387977628009452 1105 42-43 0.5674075912513104 1105 44-45 0.5744164630215209 1105 46-47 0.6192019457814979 1105 48-49 0.5875338527511644 1105 50-51 0.5807777328835755 1105 52-53 0.4417062138616217 1105 54-55 0.527416465318197 1105 56-57 0.4865378623424448 1105 58-59 0.5152434485987811 1105 60-61 0.429991427404083 1105 62-63 0.5311043173457932 1105 64-65 0.4247753294543273 1105 66-67 0.48440141654269553 1105 68-69 0.4537577566857749 1105 70-71 0.4880516008851714 1105 72-73 0.515577033608821 1105 74-75 0.47789423393277275 1105 76-77 0.4524454503550075 1105 78-79 0.5171529796737531 1105 80-81 0.4775241403902726 1105 82-83 0.4780757220081 1105 84-85 0.46655315729815783 1105 86-87 0.5708743132552883 1105 88-89 0.5164191136721641 1105 90-91 0.5020935784763196 1105 92-93 0.4112021730377933 1105 94-95 0.4213807663466014 1105 96-97 0.4367872316730157 1105 98-99 0.5089707413793363 1105 100 0.4898967003058736 1106 1 0.4976289024072216 1106 2 3.8733161336794275 1106 3 2.2067582902890024 1106 4 1.0240306606654102 1106 5 0.33553409352423813 1106 6 0.2456092457451149 1106 7 0.17476395974025394 1106 8 0.2124471760117359 1106 9 0.20406306364685634 1106 10-11 0.20205868790937842 1106 12-13 0.27452511524229806 1106 14-15 0.3046642812256195 1106 16-17 0.3068440449809344 1106 18-19 0.3183357379566232 1106 20-21 0.2602041467733329 1106 22-23 0.19933397293820576 1106 24-25 0.22702247120982122 1106 26-27 0.2732540414503788 1106 28-29 0.2634639268789556 1106 30-31 0.23618151437725032 1106 32-33 0.37227342541540764 1106 34-35 0.38688371920282094 1106 36-37 0.29237947395736086 1106 38-39 0.2781154883824968 1106 40-41 0.25716345287389686 1106 42-43 0.2823234659463054 1106 44-45 0.361623538633566 1106 46-47 0.2378795351110483 1106 48-49 0.15651843537945354 1106 50-51 0.12647731402450546 1106 52-53 0.08741135160017421 1106 54-55 0.08203081718615834 1106 56-57 0.11463709088761931 1106 58-59 0.08180679277869984 1106 60-61 0.1791315700817293 1106 62-63 0.22493341384275567 1106 64-65 0.11436037664207532 1106 66-67 0.08716749674191249 1106 68-69 0.030088343818526653 1106 70-71 0.0918205919527253 1106 72-73 0.08138767479645992 1106 74-75 0.1204322317637434 1106 76-77 0.1828228195322268 1106 78-79 0.11050812602892535 1106 80-81 0.12051070402207387 1106 82-83 0.12033162390761731 1106 84-85 0.18240074811600238 1106 86-87 0.28100363924160376 1106 88-89 0.20699686272103435 1106 90-91 0.20348106975674085 1106 92-93 0.24489878693949407 1106 94-95 0.24497188511669066 1106 96-97 0.2804252587519187 1106 98-99 0.24218858906358065 1106 100 0.16113420289132563 1107 1 0.4441682664480844 1107 2 3.5612523412511727 1107 3 2.145763468480947 1107 4 0.9984747136074432 1107 5 0.36830388149024174 1107 6 0.2858551212562901 1107 7 0.12528315300973247 1107 8 0.1899898778560356 1107 9 0.2898934517151659 1107 10-11 0.1920620718424928 1107 12-13 0.16686721354037104 1107 14-15 0.3165029897562235 1107 16-17 0.18470071758339657 1107 18-19 0.1771693055916046 1107 20-21 0.2172123289556538 1107 22-23 0.18680560946081926 1107 24-25 0.21294651450715207 1107 26-27 0.263540978367125 1107 28-29 0.19973120546716672 1107 30-31 0.15865885658405432 1107 32-33 0.274941672486797 1107 34-35 0.4297662653164025 1107 36-37 0.24766411856750636 1107 38-39 0.15586550656718856 1107 40-41 0.13281307151892463 1107 42-43 0.22261945703793629 1107 44-45 0.36801230729086143 1107 46-47 0.2671880845967962 1107 48-49 0.20213525506467533 1107 50-51 0.17162468523847707 1107 52-53 0.13798788797723205 1107 54-55 0.15367664480098853 1107 56-57 0.1742299605490487 1107 58-59 0.2711688352347892 1107 60-61 0.24714647181954064 1107 62-63 0.30167729263709475 1107 64-65 0.1687428562875013 1107 66-67 0.25384498697807345 1107 68-69 0.22900335867763033 1107 70-71 0.20052618519176235 1107 72-73 0.24589847453773928 1107 74-75 0.2778476500666187 1107 76-77 0.23345917180717635 1107 78-79 0.1619632663410968 1107 80-81 0.2944635822900139 1107 82-83 0.3548558279717611 1107 84-85 0.22317442940445886 1107 86-87 0.28939362319218276 1107 88-89 0.26505393057734494 1107 90-91 0.25478638405846965 1107 92-93 0.22809245533128575 1107 94-95 0.2113581722593878 1107 96-97 0.17772106216230554 1107 98-99 0.21695316415859622 1107 100 0.22087932500572904 1108 1 0.43785186273262866 1108 2 3.6116240816573217 1108 3 2.061418689191399 1108 4 0.9384823202747867 1108 5 0.24625661921426456 1108 6 0.1435013393143052 1108 7 0.03507132744584851 1108 8 0.009522849592286775 1108 9 0.04695023551989408 1108 10-11 0.11808987081403899 1108 12-13 0.07866656971152253 1108 14-15 0.0802649634126098 1108 16-17 0.1061598155830552 1108 18-19 0.08451306296201011 1108 20-21 0.14142010246828818 1108 22-23 0.07716078573857033 1108 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0.47818033391556014 2210 74-75 -0.0019092643110596441 2210 76-77 0.41004837969697405 2210 78-79 0.5388745774269879 2210 80-81 0.5291959995879338 2210 82-83 0.697309568081387 2210 84-85 0.6887875312539009 2210 86-87 0.7096020906041502 2210 88-89 0.7448327905035441 2210 90-91 0.7385160376791831 2210 92-93 0.745795169473773 2210 94-95 0.650592163823184 2210 96-97 0.7036385679784729 2210 98-99 0.7780939128094815 2210 100 0.742672223623412 2211 1 0.5134950332842152 2211 2 3.9050719040868884 2211 3 2.1419903425497466 2211 4 0.8976976182935203 2211 5 0.4214754013578528 2211 6 0.26449784602230864 2211 7 0.1808750543026605 2211 8 0.16603411179086436 2211 9 0.15960438229042495 2211 10-11 0.2285772527382406 2211 12-13 0.16526071155812616 2211 14-15 0.10881572356941405 2211 16-17 0.05475889523157207 2211 18-19 0.32566312905918693 2211 20-21 0.17779978617932102 2211 22-23 0.07181246264585184 2211 24-25 0.1943949466855912 2211 26-27 0.24867534239012912 2211 28-29 0.3714545684255839 2211 30-31 0.2130504309794503 2211 32-33 0.2827582999327021 2211 34-35 0.3544582033322925 2211 36-37 0.14827172957900814 2211 38-39 0.07293263903022051 2211 40-41 0.13564375706783238 2211 42-43 0.18659180858573166 2211 44-45 0.23759253594377583 2211 46-47 0.3580509242459229 2211 48-49 0.12415947712825925 2211 50-51 -0.007131807292850567 2211 52-53 0.11877835016247928 2211 54-55 0.15649053382751532 2211 56-57 0.04580224656236709 2211 58-59 0.13380888866556973 2211 60-61 0.13304129775658424 2211 62-63 0.18024047478235872 2211 64-65 0.03869385733984387 2211 66-67 0.08386066305262574 2211 68-69 0.1033910629911361 2211 70-71 0.11206603836924955 2211 72-73 0.10037302222446698 2211 74-75 0.18540374535383108 2211 76-77 0.14548135789776495 2211 78-79 0.2644730287143986 2211 80-81 0.05898584535403728 2211 82-83 -0.30147259190724895 2211 84-85 0.08595966188158144 2211 86-87 0.1498355751244631 2211 88-89 0.16706477761348282 2211 90-91 0.2032439739354217 2211 92-93 0.35228920434015976 2211 94-95 0.3723429500732536 2211 96-97 0.3091564751243041 2211 98-99 0.32365448722499934 2211 100 0.1801406980610274 2212 1 0.4815920065385271 2212 2 3.9938532411462973 2212 3 2.2444291315051785 2212 4 1.0675427921396405 2212 5 0.6233686107887024 2212 6 0.4518888729619164 2212 7 0.28779569854013687 2212 8 0.4227785378242359 2212 9 0.2534789924980103 2212 10-11 0.26181804013190657 2212 12-13 0.2681878772976063 2212 14-15 0.3175996664593086 2212 16-17 0.422533667502762 2212 18-19 0.33840897314914997 2212 20-21 0.33183336936072294 2212 22-23 0.39997320083175225 2212 24-25 0.47290822949524625 2212 26-27 0.3180766809732134 2212 28-29 0.39040917156886223 2212 30-31 0.25655275804666644 2212 32-33 0.47216657296832665 2212 34-35 0.31373279553280753 2212 36-37 0.2705738076638866 2212 38-39 0.4224143298246261 2212 40-41 0.3446690206376317 2212 42-43 0.28724514915651866 2212 44-45 0.34396788201711814 2212 46-47 0.5590330669484729 2212 48-49 0.3372766953479527 2212 50-51 0.3873267556738753 2212 52-53 0.19514757989283993 2212 54-55 0.06358704997933984 2212 56-57 0.3799625631404737 2212 58-59 0.37384201993883437 2212 60-61 0.2712980274653134 2212 62-63 0.4876133301665533 2212 64-65 0.5776482094230388 2212 66-67 0.613478826643373 2212 68-69 0.5977228406176991 2212 70-71 0.45539145465284037 2212 72-73 0.5590814306593899 2212 74-75 0.5368592878080847 2212 76-77 0.39720371661586995 2212 78-79 0.4987381628757177 2212 80-81 0.3416474631159403 2212 82-83 0.627527292951406 2212 84-85 0.5425779951543177 2212 86-87 0.37396318463427747 2212 88-89 0.5640688949987762 2212 90-91 0.46521510289199597 2212 92-93 0.49644346517600013 2212 94-95 0.5272377126137116 2212 96-97 0.3994978475745299 2212 98-99 0.4047407246308694 2212 100 0.36917595209054 2213 1 -0.8418897522766002 2213 2 -25.956711730605114 2213 3 -14.833293355361223 2213 4 -6.3808536567666 2213 5 -2.285434752623253 2213 6 -1.429430252143689 2213 7 -1.3396239381497281 2213 8 -1.1727974554170189 2213 9 -1.4306958130091587 2213 10-11 -1.3181441758342558 2213 12-13 -1.3752650343846256 2213 14-15 -1.6802789375403506 2213 16-17 -1.4574464704241024 2213 18-19 -1.4996511483211563 2213 20-21 -1.5657734125018408 2213 22-23 -1.6763287728542764 2213 24-25 -1.5895960703993524 2213 26-27 -1.6189300351181615 2213 28-29 -1.685233886268506 2213 30-31 -1.9180764534420973 2213 32-33 -1.57179090554596 2213 34-35 -1.5654587417242354 2213 36-37 -1.5955709893948509 2213 38-39 -1.509709253962093 2213 40-41 -1.6406074522654706 2213 42-43 -1.7142348745651361 2213 44-45 -1.5714984260727363 2213 46-47 -1.4619719181773334 2213 48-49 -1.76044769723174 2213 50-51 -1.6156351837230645 2213 52-53 -1.771566076022026 2213 54-55 -1.7071205544358676 2213 56-57 -1.6598671885526528 2213 58-59 -1.5698991910766011 2213 60-61 -1.554161854862052 2213 62-63 -1.770153311457527 2213 64-65 -1.6069854808359452 2213 66-67 -1.576602658079267 2213 68-69 -1.8003550376973436 2213 70-71 -1.7133757965243035 2213 72-73 -1.8943421110227554 2213 74-75 -1.880833161714584 2213 76-77 -1.7553524159282077 2213 78-79 -1.7467085927334125 2213 80-81 -1.8035087228156357 2213 82-83 -1.6758689701993887 2213 84-85 -1.661659774569472 2213 86-87 -1.7362362341575732 2213 88-89 -1.7405573100036804 2213 90-91 -1.4812489733805663 2213 92-93 -1.4620555000973638 2213 94-95 -1.725324680576911 2213 96-97 -1.733913778239696 2213 98-99 -1.4529976064871555 2213 100 -1.5999514926575316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 25.0 5 50.0 6 36.0 7 41.0 8 45.0 9 83.0 10 369.0 11 1600.0 12 4668.0 13 8700.0 14 11622.0 15 13274.0 16 14553.0 17 15042.0 18 15751.0 19 16368.0 20 18237.0 21 20800.0 22 23334.0 23 24539.0 24 25271.0 25 26388.0 26 29046.0 27 33293.0 28 38873.0 29 46310.0 30 56116.0 31 69287.0 32 87871.0 33 118264.0 34 162347.0 35 232374.0 36 353857.0 37 610865.0 38 683178.0 39 6293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.74636784240603 21.781600752830023 10.490459450300275 29.981571954463675 2 12.313818199269841 23.521967326853524 37.40807347395637 26.756140999920262 3 14.56966606230903 28.620728262989477 29.520474721718593 27.289130952982905 4 9.922531746885992 19.567704898256096 39.19558422288665 31.314179131971258 5 10.968893082270679 39.48119200736286 36.04106732500188 13.508847585364583 6 28.10337481610293 41.62352697138921 18.241179716627766 12.031918495880092 7 24.540815367961926 34.06789385677515 24.674932399524234 16.71635837573869 8 23.284111361354217 38.2070155673053 21.62895374975023 16.879919321590258 9 24.250227816853318 16.960955696216253 22.803798561483813 35.98501792544661 10-11 22.17666267699465 28.51014204737924 30.051787448341276 19.26140782728484 12-13 22.967332428339716 26.027882571477818 30.499868659097913 20.504916341084552 14-15 20.593942240810833 27.36246285038075 27.496192446319366 24.547402462489046 16-17 19.841816805275375 31.053342478972944 28.279373878962144 20.825466836789534 18-19 19.77732481977819 30.0087103293451 30.389728531079363 19.82423631979734 20-21 21.14008446836266 29.36830799920669 29.389242656703768 20.102364875726888 22-23 20.209473165145486 29.424757101803202 29.805229874988033 20.560539858063283 24-25 20.410727709697845 29.106274652458808 29.628665650615392 20.854331987227955 26-27 20.31487555929775 29.169019085567367 29.65817773053073 20.85792762460415 28-29 20.019171032558965 29.20873161602015 30.020348676541296 20.75174867487959 30-31 20.752324132777446 29.48557580889683 29.331551830865887 20.430548227459834 32-33 19.833765869777487 29.471054670788632 30.07748455031349 20.61769490912039 34-35 20.043168523385116 28.979345421868295 30.10412166935627 20.873364385390317 36-37 20.56482735928347 29.471146605101584 29.471309138073202 20.492716897541744 38-39 20.287758064921015 29.421039904313012 29.74141721993758 20.549784810828392 40-41 20.516933492643926 29.233102265552645 29.510589482900357 20.739374758903068 42-43 20.17661438704546 29.36385301602919 29.758297954109224 20.70123464281612 44-45 20.558914822902366 28.923836667186194 29.622579526021536 20.894668983889904 46-47 20.57756197014134 29.596497356328356 29.332401115626904 20.493539557903397 48-49 20.430416737489395 29.32680505834137 29.516775548520148 20.72600265564909 50-51 20.733293306485 29.25229269307716 29.11053695944789 20.903877040989947 52-53 20.30745458972697 29.509343616622342 29.297727661917367 20.885474131733325 54-55 20.67129138334181 29.31383377933761 29.04321469890421 20.971660138416368 56-57 20.73185357076675 29.2604703129363 29.03069254463075 20.976983571666207 58-59 20.262413918348113 28.964722800329216 29.61338865099357 21.159474630329097 60-61 20.87215919660489 29.252116075018126 28.90015030065549 20.975574427721494 62-63 20.23469531512461 29.556618393437056 29.2856549397277 20.923031351710637 64-65 20.34198226095036 28.85339184457434 29.49079975999217 21.313826134483136 66-67 20.484904707511774 29.211795041857314 29.161491849979804 21.141808400651104 68-69 20.691434748146495 29.181831056236312 29.14252466945424 20.984209526162953 70-71 20.847849604834078 29.10822902119913 28.866413091405406 21.17750828256138 72-73 20.379725960193294 29.34220439532293 29.363627020179717 20.91444262430406 74-75 20.76519620584585 28.804011993061668 29.09715525140796 21.333636549684524 76-77 20.652036093339262 29.33362280444769 28.977334917477254 21.037006184735795 78-79 20.6414636472266 29.222246865666897 29.191579021894455 20.944710465212047 80-81 20.868166679911283 28.990796194219087 28.92798058098121 21.21305654488842 82-83 20.2481189618685 29.393498620758656 29.232800840584115 21.125581576788726 84-85 20.459427131127867 28.845938161861636 29.101494526127166 21.593140180883335 86-87 20.637306869181245 29.210626597195255 28.91420521757976 21.23786131604374 88-89 20.344415075175775 28.878832382544395 29.351927234847636 21.42482530743219 90-91 20.676962219539735 28.87937978179381 28.995396185906536 21.448261812759924 92-93 20.186381673389803 29.28548635294122 29.216139064429232 21.31199290923975 94-95 20.178572267013973 28.68742774237658 29.35560195453598 21.77839803607346 96-97 20.402357593085128 29.000442798137506 29.131404139620827 21.465795469156546 98-99 20.35588101182387 28.82843869779369 29.177710258644428 21.637970031738003 100 20.534065320608402 28.70608329440553 28.947387450448403 21.81246393453766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.0 1 17.5 2 16.5 3 17.5 4 24.5 5 31.0 6 52.5 7 89.5 8 150.5 9 197.0 10 194.0 11 199.0 12 236.5 13 297.5 14 380.0 15 474.5 16 613.5 17 802.0 18 1081.5 19 1457.5 20 2040.0 21 2991.5 22 4432.5 23 6627.5 24 9827.0 25 14448.5 26 20880.0 27 28980.0 28 38203.0 29 48506.0 30 59391.5 31 70216.0 32 80434.0 33 89327.0 34 97440.0 35 105148.0 36 111284.0 37 116061.0 38 119209.5 39 120890.0 40 121358.5 41 119704.0 42 115821.5 43 110663.0 44 104383.0 45 97652.5 46 91536.5 47 85797.5 48 81015.5 49 77404.0 50 78619.0 51 73821.5 52 61716.0 53 54273.5 54 49247.0 55 45168.5 56 41343.0 57 37214.5 58 32796.0 59 28555.0 60 24002.0 61 19498.5 62 15708.5 63 12322.5 64 9594.5 65 7390.0 66 5502.0 67 4044.0 68 2949.0 69 2128.5 70 1590.5 71 1090.0 72 745.5 73 521.5 74 358.5 75 244.5 76 132.5 77 70.5 78 44.0 79 30.0 80 19.0 81 11.5 82 6.0 83 3.5 84 1.0 85 0.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.672642824637566 2 4.42465634683868 3 0.0 4 0.005850952405030375 5 0.16743836635630133 6 0.05804000317829513 7 0.037850605681924894 8 0.045110120702981094 9 0.04355709012633723 10-11 0.04397243551311408 12-13 0.042365229451238454 14-15 0.045326822643908145 16-17 0.03526824088587753 18-19 0.028965826103915803 20-21 0.023223224669348955 22-23 0.02329545864965797 24-25 0.01975599361451614 26-27 0.012749297524541497 28-29 0.01953929167358909 30-31 0.025444419563851225 32-33 0.0012460361603305427 34-35 0.0034672310548328144 36-37 0.004008985907150442 38-39 0.006121829831189188 40-41 0.006645526188429561 42-43 0.007440099971828747 44-45 0.008541668171541256 46-47 0.017011102362773495 48-49 0.025950057426014347 50-51 0.02304263971857641 52-53 0.028676890182679732 54-55 0.024649845780452043 56-57 0.016360996539992345 58-59 0.01464543950765319 60-61 0.011882489760833292 62-63 0.018419664978799325 64-65 0.019394823712971056 66-67 0.020442216427451802 68-69 0.028243486300825637 70-71 0.02764755596327624 72-73 0.024505377819834005 74-75 0.024541494809988518 76-77 0.01852801594926285 78-79 0.014735731983039463 80-81 0.013002116455623054 82-83 0.008270790745382442 84-85 0.011214325442974885 86-87 0.009209832489399663 88-89 0.007295632011210714 90-91 0.007440099971828747 92-93 0.0038464594514551536 94-95 0.0014807965963348478 96-97 8.668077637082036E-4 98-99 6.320473277038985E-4 100 0.10748416269981725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2768780.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.25336798094207 #Duplication Level Percentage of deduplicated Percentage of total 1 91.90562695944561 82.02886742530589 2 6.696446729474377 11.953608482211052 3 0.958123384223416 2.5654721694971427 4 0.21726276750877058 0.7756573494807265 5 0.08423069110005162 0.37589364340209847 6 0.040266660733452586 0.21563610526839563 7 0.022665366468253925 0.14160722056697808 8 0.016433269890336978 0.11733797477219055 9 0.01005410843257103 0.08076267356872993 >10 0.04496720054684064 0.6488366407387589 >50 0.0017285052976866748 0.10783160637766113 >100 0.001667526225429276 0.337531540744102 >500 2.8367804402659275E-4 0.20617079835979574 >1k 2.4315260915178382E-4 0.4447863697065813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4002 0.14454019459834297 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3230 0.11665787819906241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.6116990154508485E-5 0.0 3 0.0 0.0 0.0 3.6116990154508485E-5 0.0 4 0.0 0.0 0.0 7.223398030901697E-5 0.0 5 0.0 0.0 0.0 7.223398030901697E-5 0.0 6 0.0 0.0 0.0 7.223398030901697E-5 0.0 7 0.0 0.0 0.0 1.0835097046352545E-4 0.0 8 3.6116990154508485E-5 0.0 0.0 1.4446796061803394E-4 0.0 9 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 10-11 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 12-13 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 14-15 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 16-17 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 18-19 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 20-21 3.6116990154508485E-5 0.0 0.0 1.8058495077254242E-4 0.0 22-23 7.223398030901697E-5 0.0 0.0 2.7087742615881363E-4 0.0 24-25 1.0835097046352545E-4 0.0 0.0 3.6116990154508484E-4 0.0 26-27 1.0835097046352545E-4 0.0 0.0 4.3340388185410185E-4 0.0 28-29 1.0835097046352545E-4 0.0 0.0 9.029247538627121E-4 0.0 30-31 1.0835097046352545E-4 0.0 0.0 0.0027990667369744075 0.0 32-33 1.0835097046352545E-4 0.0 0.0 0.006916403614588375 0.0 34-35 1.264094655407797E-4 0.0 0.0 0.012695122039309732 0.0 36-37 1.6252645569528817E-4 0.0 0.0 0.022609235836722308 0.0 38-39 1.8058495077254242E-4 0.0 0.0 0.039656455189650316 0.0 40-41 2.167019409270509E-4 0.0 0.0 0.06221151554114086 0.0 42-43 2.889359212360679E-4 0.0 0.0 0.0857597931218804 1.8058495077254243E-5 44-45 2.889359212360679E-4 0.0 0.0 0.11257665831160293 3.6116990154508485E-5 46-47 3.431114064678306E-4 0.0 0.0 0.14699614992884952 3.6116990154508485E-5 48-49 3.6116990154508484E-4 0.0 0.0 0.18338401750951683 3.6116990154508485E-5 50-51 3.6116990154508484E-4 0.0 0.0 0.22331135012532596 3.6116990154508485E-5 52-53 3.6116990154508484E-4 0.0 0.0 0.26672397229104516 3.6116990154508485E-5 54-55 3.6116990154508484E-4 0.0 0.0 0.31526520705870453 3.6116990154508485E-5 56-57 3.6116990154508484E-4 0.0 1.8058495077254243E-5 0.3737747311090083 3.6116990154508485E-5 58-59 3.6116990154508484E-4 0.0 3.6116990154508485E-5 0.44037446095392196 3.6116990154508485E-5 60-61 3.6116990154508484E-4 0.0 3.6116990154508485E-5 0.5089064497721019 3.6116990154508485E-5 62-63 3.6116990154508484E-4 0.0 3.6116990154508485E-5 0.5826573436676081 3.6116990154508485E-5 64-65 3.6116990154508484E-4 0.0 3.6116990154508485E-5 0.6636496940890935 3.6116990154508485E-5 66-67 3.6116990154508484E-4 0.0 5.417548523176273E-5 0.7569398796581888 3.6116990154508485E-5 68-69 3.6116990154508484E-4 0.0 7.223398030901697E-5 0.8654533765774095 3.6116990154508485E-5 70-71 3.792283966223391E-4 0.0 7.223398030901697E-5 0.9891721263516784 3.6116990154508485E-5 72-73 4.334038818541018E-4 0.0 7.223398030901697E-5 1.1187960040162093 3.6116990154508485E-5 74-75 4.334038818541018E-4 0.0 7.223398030901697E-5 1.2528622714697448 5.417548523176273E-5 76-77 4.334038818541018E-4 0.0 7.223398030901697E-5 1.4041924602171352 7.223398030901697E-5 78-79 4.334038818541018E-4 0.0 7.223398030901697E-5 1.579034809555111 7.223398030901697E-5 80-81 4.334038818541018E-4 0.0 7.223398030901697E-5 1.7731455731405168 7.223398030901697E-5 82-83 4.334038818541018E-4 0.0 7.223398030901697E-5 1.9833645143348333 7.223398030901697E-5 84-85 4.334038818541018E-4 0.0 7.223398030901697E-5 2.196617282702129 7.223398030901697E-5 86-87 4.334038818541018E-4 0.0 7.223398030901697E-5 2.427747961195906 7.223398030901697E-5 88 4.334038818541018E-4 0.0 7.223398030901697E-5 2.6205765716308265 7.223398030901697E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8210 0.0 20.82352 1 CAACGCA 8845 0.0 19.306713 5 AACGCAG 8845 0.0 19.221174 6 CTGTCGC 440 0.0 19.210297 9 ATCAACG 8935 0.0 19.005907 3 TCAACGC 8915 0.0 18.944042 4 TCTGTCG 440 0.0 18.14273 8 TATCAAC 9370 0.0 17.96463 2 TATACCG 215 2.6037336E-5 17.504555 5 ACGCAGA 9700 0.0 17.282352 7 CGCAGAG 9710 0.0 17.216194 8 GAGTACT 3645 0.0 17.198483 12-13 ATACGCC 195 2.4520024E-4 16.88649 3 GTATTAA 1790 0.0 16.514523 1 GTTCGGG 185 0.0024646223 15.978917 1 TACATGG 10100 0.0 15.740295 2 GTACATG 10015 0.0 15.643862 1 GCAGAGT 10815 0.0 15.023247 9 ACATGGG 9920 0.0 14.889975 3 AGTACTT 4560 0.0 14.88022 12-13 >>END_MODULE