FastQCFastQC Report
Wed 25 May 2016
SRR1295078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643924
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG2451.8730316E-417.4919432
GTACATG102300.016.940831
GTATTAC8400.016.0386331
TACATGG104450.015.8256462
GTACGGC2005.4379395E-415.20911494
ACATGGG103400.014.7652293
GTATAGA11650.014.7182441
GTACTAG6304.674803E-1014.5805731
GTATATA22500.014.1528781
GTCCTAG9100.014.131941
GTATAGC6104.642061E-914.0547171
GTATAAG10950.013.9813721
TACACTG14750.013.8227125
GCGTACG950.006716098613.74248192-93
ATATCGC2200.007122018413.6207026
TTATACT18900.013.5128024
CATGGGG32500.013.4956284
CGTGTAC2550.00938869113.0628853
GTATCAA74600.013.0521551
GTATATG15650.012.9128911
Produced by FastQC (version 0.11.5)