FastQCFastQC Report
Wed 25 May 2016
SRR1295078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643924
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60570.2290913051963672No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47090.17810648112426833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108850.017.7302571
CTGTCGC3501.5279511E-917.4427059
GAGTACT38800.016.88453912-13
CATGGGG38000.016.3406374
AGTACTT45150.016.22607812-13
GTACATG136150.016.127781
CAACGCA118550.016.0309225
ATCAACG120950.016.0230543
TAGACGA2355.5832574E-516.0140364
TCAACGC120650.015.9858314
AACGCAG118800.015.973276
TATCAAC120800.015.9617482
TACATGG136950.015.6238662
ACATGGG129250.015.4308313
GTACTAG6150.015.2102341
GTCCTAG10000.014.7699381
CGCAGAG128850.014.6517118
ACGCAGA128600.014.6436767
CTATGCG2257.3755265E-414.6101289
CATGGGT21550.014.188774
Produced by FastQC (version 0.11.5)