##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295077_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 212104 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.85548127333761 27.0 15.0 33.0 6.0 33.0 2 20.9986280315317 27.0 6.0 33.0 2.0 33.0 3 23.45401312563648 27.0 15.0 33.0 6.0 33.0 4 23.19065647041074 27.0 6.0 37.0 2.0 37.0 5 25.41791291064761 33.0 15.0 37.0 2.0 37.0 6 25.930581224305058 33.0 15.0 37.0 6.0 37.0 7 25.822620035454307 33.0 15.0 37.0 6.0 37.0 8 26.607551012710747 33.0 15.0 37.0 6.0 37.0 9 27.247567231169615 33.0 15.0 37.0 6.0 37.0 10-11 27.05117536680119 33.0 15.0 37.0 6.0 37.0 12-13 25.951295119375402 33.0 15.0 37.0 4.0 37.0 14-15 27.413700354543053 33.0 15.0 37.0 6.0 40.0 16-17 27.747484724474788 33.0 15.0 37.0 6.0 40.0 18-19 27.889471674272997 33.0 15.0 37.0 6.0 40.0 20-21 27.39924753894316 33.0 15.0 37.0 6.0 40.0 22-23 28.240396220721912 33.0 15.0 37.0 6.0 40.0 24-25 28.415220835062044 33.0 18.5 37.0 6.0 40.0 26-27 26.863194470637048 33.0 15.0 37.0 4.0 40.0 28-29 27.540607437860672 33.0 15.0 37.0 6.0 40.0 30-31 28.138684324670916 33.0 22.0 37.0 6.0 40.0 32-33 26.810241673895824 33.0 15.0 37.0 6.0 40.0 34-35 26.462810696639384 33.0 15.0 37.0 6.0 40.0 36-37 26.780746709161544 33.0 15.0 37.0 6.0 40.0 38-39 26.77439840832799 33.0 15.0 37.0 6.0 40.0 40-41 25.74704154565685 33.0 15.0 37.0 6.0 40.0 42-43 26.160880982914044 33.0 15.0 37.0 6.0 40.0 44-45 25.895716252404483 30.0 15.0 37.0 6.0 40.0 46-47 25.45652368649342 27.0 15.0 37.0 6.0 40.0 48-49 25.215488628220122 27.0 15.0 37.0 6.0 37.0 50-51 25.282948459246406 27.0 15.0 37.0 6.0 37.0 52-53 25.640612623995775 27.0 15.0 37.0 6.0 38.5 54-55 25.47721636555652 27.0 15.0 37.0 6.0 37.0 56-57 25.212673971259385 27.0 15.0 37.0 6.0 37.0 58-59 25.07857701882096 27.0 15.0 37.0 6.0 37.0 60-61 24.95066571116056 27.0 15.0 35.0 6.0 37.0 62-63 24.610495323049072 27.0 15.0 33.0 6.0 37.0 64-65 24.31966393844529 27.0 15.0 33.0 6.0 37.0 66-67 24.518703089050653 27.0 15.0 33.0 6.0 37.0 68-69 23.9950519556444 27.0 15.0 33.0 6.0 37.0 70-71 23.867854920227813 27.0 15.0 33.0 6.0 37.0 72-73 23.660284577377134 27.0 15.0 33.0 6.0 37.0 74-75 23.678829725040544 27.0 15.0 33.0 6.0 37.0 76-77 23.278134782936675 27.0 15.0 33.0 6.0 37.0 78-79 22.941189227925925 27.0 15.0 33.0 6.0 37.0 80-81 22.911201580356806 27.0 15.0 33.0 6.0 33.0 82-83 23.160690038848866 27.0 15.0 33.0 6.0 33.0 84-85 22.906126239957757 27.0 15.0 33.0 6.0 33.0 86-87 22.441224587937995 27.0 15.0 33.0 6.0 33.0 88-89 22.351002338475467 27.0 15.0 33.0 6.0 33.0 90-91 22.482866895485234 27.0 15.0 33.0 6.0 33.0 92-93 22.21453626522838 24.5 15.0 33.0 6.0 33.0 94-95 22.198016067589485 27.0 15.0 33.0 6.0 33.0 96-97 22.40399992456531 27.0 15.0 33.0 6.0 33.0 98-99 22.36719958133746 27.0 15.0 33.0 6.0 33.0 100 21.811252970241014 22.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.385713005916173 1101 2 9.7753147757069 1101 3 7.6229301675630765 1101 4 11.651342154256458 1101 5 9.280223527704266 1101 6 8.77041991794848 1101 7 8.47269701039674 1101 8 8.015323509657438 1101 9 6.213873958991275 1101 10-11 6.498350683298941 1101 12-13 8.008843609660882 1101 14-15 6.712029067043666 1101 16-17 7.008723839206514 1101 18-19 6.891675369324407 1101 20-21 6.528226670842532 1101 22-23 6.303568773535762 1101 24-25 6.014968675477103 1101 26-27 7.160770142407415 1101 28-29 6.850129685208785 1101 30-31 3.9964456742969183 1101 32-33 4.998924317176744 1101 34-35 7.578256429474134 1101 36-37 6.869285164306394 1101 38-39 6.8703006309147945 1101 40-41 7.484453798663662 1101 42-43 7.397441778174393 1101 44-45 7.068043181116948 1101 46-47 6.012443257340838 1101 48-49 4.085516234915293 1101 50-51 5.377535522356819 1101 52-53 4.750158087797274 1101 54-55 2.7711238743826527 1101 56-57 4.658297437418007 1101 58-59 4.790169230190923 1101 60-61 5.413727825884948 1101 62-63 4.56871514726901 1101 64-65 4.611556866933512 1101 66-67 4.033625480056742 1101 68-69 5.017029132336859 1101 70-71 4.532439035329343 1101 72-73 4.442849256544502 1101 74-75 3.620521834983581 1101 76-77 3.8368676255807763 1101 78-79 3.8628517312091795 1101 80-81 3.255174810865743 1101 82-83 3.20687555647692 1101 84-85 3.3655291589543843 1101 86-87 2.9023929714546846 1101 88-89 2.8368707599060876 1101 90-91 2.773844111695471 1101 92-93 2.725015595002265 1101 94-95 2.7650280034272434 1101 96-97 3.0125341974412727 1101 98-99 2.706386436698992 1101 100 2.8748118377037386 1102 1 6.607050786963104 1102 2 9.969057367405856 1102 3 7.664215194478928 1102 4 12.065026369714449 1102 5 9.92304620167343 1102 6 9.028680905659485 1102 7 6.198703485193288 1102 8 7.989466489281977 1102 9 5.970909795256695 1102 10-11 7.322416124657995 1102 12-13 8.37868005311417 1102 14-15 5.807867300201931 1102 16-17 6.52758367532201 1102 18-19 7.20734278404403 1102 20-21 6.333467414272864 1102 22-23 6.115485772453503 1102 24-25 6.501260097542946 1102 26-27 7.850223532773928 1102 28-29 7.564139983040057 1102 30-31 3.7718072462388434 1102 32-33 4.499577887450773 1102 34-35 4.90442781949395 1102 36-37 5.052338719588132 1102 38-39 4.302390228605979 1102 40-41 6.416044434102634 1102 42-43 6.696418554175477 1102 44-45 6.627302561841546 1102 46-47 4.487780935315758 1102 48-49 2.9331992228792956 1102 50-51 4.746123388906671 1102 52-53 5.3322713452008905 1102 54-55 5.403524562154093 1102 56-57 6.147962125414335 1102 58-59 5.281398738771667 1102 60-61 5.159458536191682 1102 62-63 4.074885412866848 1102 64-65 4.980564049178888 1102 66-67 2.770487312021249 1102 68-69 5.370634076045484 1102 70-71 4.818833997918887 1102 72-73 4.405195175595491 1102 74-75 3.0623634507779585 1102 76-77 2.4762163041507 1102 78-79 3.789473827038041 1102 80-81 3.941697676455153 1102 82-83 3.3178278950403772 1102 84-85 2.28801197638105 1102 86-87 2.9202853361609513 1102 88-89 2.966350293083565 1102 90-91 2.9949991741711663 1102 92-93 2.974053979957496 1102 94-95 2.819171264157273 1102 96-97 2.7866963859412444 1102 98-99 2.79023786307512 1102 100 3.01813815901669 1103 1 6.494508030726951 1103 2 10.774793423542825 1103 3 7.9207572462220845 1103 4 11.801212281708445 1103 5 10.183801306930246 1103 6 9.59002741424781 1103 7 7.9938397026220755 1103 8 7.688221575999503 1103 9 7.124322414950981 1103 10-11 7.475508941610318 1103 12-13 8.736495290556118 1103 14-15 8.277335407989359 1103 16-17 7.958466887068504 1103 18-19 7.190784881208444 1103 20-21 8.221501693418947 1103 22-23 6.749478486512771 1103 24-25 5.176196832217904 1103 26-27 5.316440312684755 1103 28-29 6.729350543019294 1103 30-31 6.148228977993867 1103 32-33 7.5300541617951815 1103 34-35 6.90560274695898 1103 36-37 4.122792654088823 1103 38-39 6.664063955652022 1103 40-41 6.646612941411746 1103 42-43 7.1556298001084215 1103 44-45 5.349894539891025 1103 46-47 6.076720858748491 1103 48-49 5.0333554163924 1103 50-51 6.208845512767663 1103 52-53 5.967531683172137 1103 54-55 4.778106269437256 1103 56-57 5.810737086250086 1103 58-59 5.223319779938162 1103 60-61 4.663048206099631 1103 62-63 2.654856955057639 1103 64-65 5.199809685493648 1103 66-67 4.204049451084458 1103 68-69 5.153397635218262 1103 70-71 3.2148471237357725 1103 72-73 4.237732091955486 1103 74-75 2.934752887090866 1103 76-77 3.0531682595822467 1103 78-79 2.579729576535165 1103 80-81 3.7935872935261266 1103 82-83 2.991054380269201 1103 84-85 2.760430148592512 1103 86-87 2.6571363451617778 1103 88-89 2.5259749651698833 1103 90-91 2.3081509460637513 1103 92-93 2.2346978494779783 1103 94-95 3.161853806635417 1103 96-97 2.864128045485735 1103 98-99 3.1340860633736014 1103 100 2.741550500621635 1104 1 5.421946114817395 1104 2 8.276075403365574 1104 3 6.251842470011173 1104 4 10.665991279534651 1104 5 8.284148742041626 1104 6 7.027535542743063 1104 7 3.5496894322915473 1104 8 5.741544504858911 1104 9 6.0997046864705915 1104 10-11 6.310625899044872 1104 12-13 6.173599316715936 1104 14-15 6.28602593062838 1104 16-17 6.345555120723262 1104 18-19 5.575824734059452 1104 20-21 6.660073493578725 1104 22-23 5.688760949400386 1104 24-25 5.027580069867241 1104 26-27 7.285463778489337 1104 28-29 6.392431382065794 1104 30-31 5.293515379718034 1104 32-33 6.557152242541328 1104 34-35 7.171348803477571 1104 36-37 5.8457503115958325 1104 38-39 6.5267481241301795 1104 40-41 5.97019672529596 1104 42-43 5.750175812036407 1104 44-45 5.473819256090419 1104 46-47 5.7955399509581405 1104 48-49 4.194257818711108 1104 50-51 4.5151504381713 1104 52-53 4.99963691699411 1104 54-55 3.882922054510548 1104 56-57 5.136884829779802 1104 58-59 3.1721381289711026 1104 60-61 3.180257391763753 1104 62-63 2.899729035766626 1104 64-65 2.291048561067285 1104 66-67 2.4169231296233704 1104 68-69 2.953413427723323 1104 70-71 1.7517040436399043 1104 72-73 1.3265788674975383 1104 74-75 1.9393234238502437 1104 76-77 2.5582079266390814 1104 78-79 2.9234859632100054 1104 80-81 2.250153259748746 1104 82-83 1.6942899858653213 1104 84-85 1.9408641637904722 1104 86-87 0.2165533230336898 1104 88-89 -0.7243315952225124 1104 90-91 1.1833622869403264 1104 92-93 1.5386571233943336 1104 94-95 1.3468119100484586 1104 96-97 1.7354098395143076 1104 98-99 1.296563071344174 1104 100 -0.21787205107559515 1105 1 5.078097490323611 1105 2 -2.6625225578130305 1105 3 0.6427794899804482 1105 4 7.909560613426567 1105 5 8.246360723608447 1105 6 7.409451476271901 1105 7 5.44534646343245 1105 8 5.350928669081785 1105 9 4.753691559812303 1105 10-11 4.877027239639762 1105 12-13 4.348560914258179 1105 14-15 5.36739724748357 1105 16-17 5.6624103085461925 1105 18-19 5.961154995196168 1105 20-21 6.440383506146805 1105 22-23 1.8354723025633497 1105 24-25 3.589819980494248 1105 26-27 6.863853668169547 1105 28-29 5.678459869707186 1105 30-31 5.084585062723203 1105 32-33 6.406677448518149 1105 34-35 5.58478268142758 1105 36-37 6.055322996974976 1105 38-39 3.7619457222310047 1105 40-41 4.736395580204491 1105 42-43 4.840135314899133 1105 44-45 5.308632829613671 1105 46-47 4.41601893434035 1105 48-49 4.329504292667263 1105 50-51 5.002938456604479 1105 52-53 4.885721262755872 1105 54-55 4.895001373041481 1105 56-57 4.838790988137571 1105 58-59 1.7103381010420442 1105 60-61 4.012752853291474 1105 62-63 -0.7469543883361496 1105 64-65 -0.27188259379935076 1105 66-67 0.7375696873667046 1105 68-69 2.476035966474896 1105 70-71 2.2662062081420693 1105 72-73 2.194746721149265 1105 74-75 1.8154580419203477 1105 76-77 1.3810329510770103 1105 78-79 2.6264045499995596 1105 80-81 1.0534977647061545 1105 82-83 0.42997634251942074 1105 84-85 1.2842798878513584 1105 86-87 0.18963669127835203 1105 88-89 0.9215977442552123 1105 90-91 0.5615636554642762 1105 92-93 1.4994447228916101 1105 94-95 0.9223111419993053 1105 96-97 1.8509733083035833 1105 98-99 1.9271314277189795 1105 100 2.138726189393612 1106 1 6.3715966821150225 1106 2 10.131082052489873 1106 3 6.396100173092403 1106 4 11.678497292040664 1106 5 9.82134139688134 1106 6 9.05892789766483 1106 7 6.539927951941831 1106 8 7.2745213319533875 1106 9 6.794467440056874 1106 10-11 5.7771256302504845 1106 12-13 8.19787569393349 1106 14-15 7.898580043182495 1106 16-17 8.265298775761039 1106 18-19 7.2497276285505805 1106 20-21 8.342330234658998 1106 22-23 6.8277135001176426 1106 24-25 5.47433048720589 1106 26-27 7.5677049577893385 1106 28-29 6.490463524215585 1106 30-31 5.208880515671407 1106 32-33 7.465585312739105 1106 34-35 6.956214264813621 1106 36-37 6.471128745797802 1106 38-39 7.584772343919106 1106 40-41 7.034940804745922 1106 42-43 6.151415095628632 1106 44-45 4.641164982259674 1106 46-47 6.111394575636293 1106 48-49 5.851361064049652 1106 50-51 6.291138611218297 1106 52-53 5.181490466494715 1106 54-55 5.0068504137330265 1106 56-57 4.948679244515393 1106 58-59 5.573525956829517 1106 60-61 5.3771846475145395 1106 62-63 4.9949529839317535 1106 64-65 5.23061933887336 1106 66-67 3.138926072214552 1106 68-69 3.3651356923867652 1106 70-71 3.122633223392615 1106 72-73 2.7466266846041805 1106 74-75 2.5484680918184424 1106 76-77 3.809594339790202 1106 78-79 3.886760161778323 1106 80-81 3.4831484780380855 1106 82-83 1.9696839818475524 1106 84-85 2.859843876887833 1106 86-87 3.5749764902803847 1106 88-89 3.3555003375505983 1106 90-91 3.131371793773365 1106 92-93 2.759209991006024 1106 94-95 1.5241383961984738 1106 96-97 1.6684446696079611 1106 98-99 2.071695346476446 1106 100 3.309757182435991 1107 1 5.9527014291548 1107 2 9.91776953013433 1107 3 5.540755049968528 1107 4 10.80942993043599 1107 5 9.302627775446034 1107 6 7.860610428056756 1107 7 4.139144753090154 1107 8 6.446267642417872 1107 9 5.4740666429221 1107 10-11 4.755641082866024 1107 12-13 6.127046367177915 1107 14-15 6.1842943288967795 1107 16-17 6.60878442872869 1107 18-19 6.161645629581841 1107 20-21 5.515019542554629 1107 22-23 5.704784726618193 1107 24-25 4.437381711012165 1107 26-27 6.760701227607381 1107 28-29 6.234665019075553 1107 30-31 5.181881988233897 1107 32-33 5.02069491877057 1107 34-35 6.5826688267378906 1107 36-37 6.484456133137478 1107 38-39 6.340940151008777 1107 40-41 7.272952427305395 1107 42-43 5.557793567415356 1107 44-45 4.005672974511302 1107 46-47 3.2753971584272854 1107 48-49 3.5096290146793194 1107 50-51 4.674176735257298 1107 52-53 3.9006351724699755 1107 54-55 4.66068906362084 1107 56-57 4.806184435646575 1107 58-59 2.7491394858232034 1107 60-61 2.034395790678275 1107 62-63 0.8425474114208242 1107 64-65 1.9439690236892417 1107 66-67 3.5382483218440868 1107 68-69 4.258769762930406 1107 70-71 3.8520626402671567 1107 72-73 2.5137759987245225 1107 74-75 2.1616533327473206 1107 76-77 2.277323388291858 1107 78-79 3.3779991117530024 1107 80-81 2.0194399432145325 1107 82-83 0.9232587782669484 1107 84-85 1.3110263399227975 1107 86-87 1.5732600491434 1107 88-89 1.849229172368105 1107 90-91 0.9371259362263658 1107 92-93 1.2158878528638688 1107 94-95 1.2943954210648059 1107 96-97 1.4256437349720876 1107 98-99 0.9746014508750918 1107 100 0.2360918954256661 1108 1 4.063870856742138 1108 2 -3.016132388842216 1108 3 -0.03716851899981677 1108 4 6.417040230583602 1108 5 6.240925298818183 1108 6 6.577618792826314 1108 7 4.3069321895343045 1108 8 4.975577138891545 1108 9 4.577560164326069 1108 10-11 3.4354344238533976 1108 12-13 2.709496752613372 1108 14-15 3.835942680545134 1108 16-17 4.31268798785613 1108 18-19 4.076374184608905 1108 20-21 4.6293708628760974 1108 22-23 3.6458705065099437 1108 24-25 2.6842900265771945 1108 26-27 4.5054937485193065 1108 28-29 5.918788358422773 1108 30-31 4.256935293137946 1108 32-33 4.1661599015489905 1108 34-35 3.002317834446604 1108 36-37 1.2412381570836821 1108 38-39 2.172119419501474 1108 40-41 5.364718869818102 1108 42-43 2.7506753125359076 1108 44-45 2.8365898188609826 1108 46-47 4.420698126116317 1108 48-49 4.705446629899921 1108 50-51 1.77650574952661 1108 52-53 0.37311344047063244 1108 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2212 20-21 -22.05854122503599 2212 22-23 -17.272611011971577 2212 24-25 -23.658567116279947 2212 26-27 -15.576923833898277 2212 28-29 -16.735856996222584 2212 30-31 -5.2503574141547595 2212 32-33 -15.592781157392071 2212 34-35 -12.64033950821074 2212 36-37 -15.944376228530704 2212 38-39 -21.850957503575447 2212 40-41 -8.647768642669408 2212 42-43 -12.237336675476081 2212 44-45 -15.187186404915241 2212 46-47 -14.826254920836732 2212 48-49 -15.52552240106911 2212 50-51 -7.408009734988873 2212 52-53 -1.144468977111785 2212 54-55 -21.07811690652841 2212 56-57 -20.73432729143232 2212 58-59 -10.387052680219707 2212 60-61 -5.372689661183298 2212 62-63 -2.0308403948028015 2212 64-65 -14.749993729975003 2212 66-67 -15.106636643936401 2212 68-69 -12.401398427088532 2212 70-71 -7.513280971345111 2212 72-73 -14.340504049585377 2212 74-75 -5.044193059666238 2212 76-77 -8.03328391485276 2212 78-79 -3.026230986506942 2212 80-81 -1.1032433436478577 2212 82-83 -1.4433224017470643 2212 84-85 -3.728300005373697 2212 86-87 -3.6967833403029715 2212 88-89 -2.7148410857320044 2212 90-91 -3.780507176929138 2212 92-93 -4.592878008140797 2212 94-95 -2.706051892815342 2212 96-97 -5.03640168229721 2212 98-99 -2.4187216439405432 2212 100 -2.119969953173495 2213 1 -19.8745906817194 2213 2 -15.873275245985074 2213 3 -18.8195861014174 2213 4 -17.580761967218596 2213 5 -19.906327448434563 2213 6 -20.442161426953906 2213 7 -9.67758329498118 2213 8 -9.78226501895061 2213 9 -3.7336286468627407 2213 10-11 -16.59088925247028 2213 12-13 -15.059084665968047 2213 14-15 -22.101419956817505 2213 16-17 -16.618730593562454 2213 18-19 -17.284564876330155 2213 20-21 -16.331268497763265 2213 22-23 -18.474658964019525 2213 24-25 -6.694281401994232 2213 26-27 -21.73076998774443 2213 28-29 -22.606486366851954 2213 30-31 -17.942415356212702 2213 32-33 -21.599774164385078 2213 34-35 -16.03644340431464 2213 36-37 -22.003816787971264 2213 38-39 -9.9516068542248 2213 40-41 -15.095820590721356 2213 42-43 -21.139434577573983 2213 44-45 -14.930942648671486 2213 46-47 -14.752079096660907 2213 48-49 -4.818729194275903 2213 50-51 -21.04437337135251 2213 52-53 -15.954908537551347 2213 54-55 -15.36707794548945 2213 56-57 -15.34471690182193 2213 58-59 -15.266173559340587 2213 60-61 -16.488573777067415 2213 62-63 -15.132738496700906 2213 64-65 -15.102890832872106 2213 66-67 -7.314678601978359 2213 68-69 -19.873426399116504 2213 70-71 -14.660633618697759 2213 72-73 -7.089005548086874 2213 74-75 -2.9632739787471607 2213 76-77 -2.550017181586025 2213 78-79 -3.8676395779155364 2213 80-81 -2.253093493498003 2213 82-83 -2.140125598550263 2213 84-85 -2.0764518535255405 2213 86-87 -2.8094207029403364 2213 88-89 -2.6366692575601753 2213 90-91 -1.5482394446614016 2213 92-93 -1.934286599549388 2213 94-95 -2.785222721986173 2213 96-97 -2.2299581258536527 2213 98-99 -2.5346057598246574 2213 100 -2.958131791335333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62.0 3 83.0 4 461.0 5 1234.0 6 1845.0 7 2017.0 8 1960.0 9 2187.0 10 2846.0 11 3712.0 12 5162.0 13 6352.0 14 6727.0 15 7092.0 16 6788.0 17 6370.0 18 6113.0 19 5782.0 20 5670.0 21 5707.0 22 5994.0 23 6241.0 24 6623.0 25 6865.0 26 6875.0 27 7037.0 28 7376.0 29 7731.0 30 8264.0 31 9148.0 32 10428.0 33 11320.0 34 12219.0 35 12222.0 36 9967.0 37 4840.0 38 783.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15826648864065 21.584295659086973 10.939936603712045 23.317501248560333 2 12.517560674080134 28.663390755436787 31.431889559749997 27.387159010733086 3 22.796378459042334 34.23487936542911 23.91959698338458 19.04914519214398 4 13.954006210027586 24.91371525415342 26.166854287022034 34.965424248796964 5 11.182117197602606 43.85278667589567 30.733115485944552 14.231980640557174 6 30.612117165985147 32.14814737544855 18.507468914295252 18.732266544271052 7 21.759126773230857 37.82756224193614 20.162196966599893 20.251114018233103 8 28.120400994795276 35.59828731123247 19.88411147860421 16.39720021536805 9 26.94501868490101 22.329947523256738 17.73624473244812 32.98878905939413 10-11 21.790922005142296 34.46044998877173 20.960534516212867 22.788093489873106 12-13 26.166342900112795 31.987613318293857 21.21610895266742 20.62993482892593 14-15 27.2945314998401 30.48867772244811 20.125786163522015 22.091004614189778 16-17 21.11830545560262 34.115558910460585 26.40712622631838 18.359009407618412 18-19 23.707672944598944 32.59877254993581 26.190207028571 17.503347476894245 20-21 21.891363514394143 32.1697908281264 25.235557940736765 20.703287716742693 22-23 19.821602585989897 33.41424936259935 21.287063321362083 25.477084730048677 24-25 21.63549906608821 34.66115001376683 18.931991873851214 24.771359046293746 26-27 24.069734745524414 29.587798502410934 20.956613657429106 25.385853094635547 28-29 23.66218303643075 28.635301316000643 25.144439094848337 22.55807655272027 30-31 25.903291624671205 32.02512352811033 22.71073526881192 19.36084957840655 32-33 19.25076570663784 31.850564949939514 25.653617481275575 23.24505186214707 34-35 16.620839473164505 32.96367751834354 26.342027840653024 24.07345516783893 36-37 23.927712849283207 31.492940550276348 22.810712907302626 21.768633693137815 38-39 26.177701255280382 28.318384752447496 22.3155118220585 23.188402170213617 40-41 21.488286136406927 32.2371909377783 22.344111151721687 23.930411774093084 42-43 25.60740106425456 30.22678817617707 22.59896085763147 21.5668499019369 44-45 27.568134698118985 28.19589259090065 22.133172022228802 22.10280068875156 46-47 22.128810987025048 30.548053257716827 27.721420405473545 19.60171534978458 48-49 24.407467393248574 29.704425893301252 26.818517057441298 19.06958965600888 50-51 21.131728031072964 30.347206150192697 26.18837983879894 22.3326859799354 52-53 19.344644782114326 31.46950388123471 22.024591818150494 27.16125951850047 54-55 21.212555681351876 32.8632451166033 19.793886298803066 26.130312903241755 56-57 24.023554296954153 28.142590972664955 21.141291186660144 26.69256354372075 58-59 23.49934680029638 26.684426549155717 25.177192606169324 24.639034044378583 60-61 25.597003965339994 29.759631859793412 22.90130709355265 21.74205708131395 62-63 19.622810847523407 30.187745597438507 25.077620918837628 25.111822636200454 64-65 17.311741529539972 31.291020893226097 25.88205707297589 25.515180504258034 66-67 23.535626048571526 29.671129290379977 22.65881476968897 24.13442989135953 68-69 26.341233987251687 26.93607277678074 21.969181261216658 24.753511974750914 70-71 22.11106337582846 30.132119462640166 22.084513977051664 25.672303184479706 72-73 25.68551647584006 28.635234900934435 22.538671693707744 23.14057692951776 74-75 27.32281724676213 26.820772716148824 21.612625006236946 24.2437850308521 76-77 22.475303892540072 29.207509236475204 26.41762922132524 21.89955764965948 78-79 24.5929613364127 28.6390560867328 25.96551270238252 20.802469874471978 80-81 21.849475614510485 29.24020657756044 25.297087602939833 23.613230204989243 82-83 20.114173134895204 30.323530109900542 22.15979491561632 27.402501839587934 84-85 21.879192072262686 30.974693286251075 20.19342802204479 26.952686619441447 86-87 23.965042596051195 27.296269206096714 21.58752251708458 27.15116568076751 88-89 23.852664881620107 25.966735522979906 25.09682177914911 25.08377781625088 90-91 25.421450507914006 28.47460429955115 23.110323647531303 22.993621545003542 92-93 20.65105132925737 29.151728424530464 24.544408232911323 25.652812013300846 94-95 18.660168041740317 30.151787279818404 25.3714120529431 25.816632625498176 96-97 23.679977637379686 28.825038554199743 22.867437513769357 24.62754629465121 98-99 25.419313278293288 26.808103486659185 22.353506790009426 25.419076445038108 100 22.026862835017187 29.262146662947536 24.338239117998857 24.372751384036423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2162.0 1 2165.5 2 1765.0 3 1132.5 4 713.5 5 438.0 6 314.0 7 279.5 8 278.0 9 267.0 10 262.5 11 263.5 12 261.0 13 294.0 14 342.5 15 389.0 16 470.0 17 536.5 18 583.0 19 617.0 20 649.5 21 712.5 22 812.0 23 895.5 24 969.5 25 1090.5 26 1243.5 27 1466.0 28 1673.5 29 1834.5 30 1990.5 31 2152.5 32 2324.0 33 2503.0 34 2665.5 35 2768.5 36 2885.5 37 3069.5 38 3256.5 39 3359.5 40 3418.5 41 3461.0 42 3524.0 43 3618.5 44 3840.0 45 4401.5 46 5510.0 47 7503.0 48 10341.5 49 15027.0 50 21305.5 51 21375.5 52 14848.0 53 9474.5 54 6544.5 55 4932.0 56 3932.0 57 3245.0 58 2662.0 59 2161.5 60 1791.5 61 1511.5 62 1314.5 63 1149.0 64 914.0 65 753.5 66 645.5 67 497.0 68 375.5 69 295.0 70 225.0 71 173.5 72 144.5 73 112.0 74 83.5 75 61.0 76 41.0 77 27.5 78 23.5 79 18.5 80 14.5 81 9.5 82 5.0 83 4.0 84 3.5 85 4.0 86 2.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.4859502885376985 2 24.490344359370873 3 5.85090333044167 4 23.77607211556595 5 12.133198808131858 6 9.606608079055558 7 7.739599441783277 8 8.056896616754045 9 5.125787349602081 10-11 6.573897710557085 12-13 9.715988383057368 14-15 7.12056349715234 16-17 7.5620921811941315 18-19 6.163721570550297 20-21 7.7563365141628635 22-23 5.414560781503414 24-25 4.9657243616339155 26-27 9.458567495191037 28-29 5.993286312375061 30-31 4.106004601516237 32-33 8.410496737449554 34-35 6.894259419907216 36-37 6.550795836004979 38-39 5.802813714027081 40-41 8.414268472070304 42-43 6.127890091653152 44-45 6.083336476445518 46-47 5.560951231471354 48-49 3.792243427752423 50-51 6.292196281069663 52-53 4.218213706483612 54-55 4.637819183042281 56-57 4.602930637800324 58-59 3.2814091200543127 60-61 3.6934711273714784 62-63 2.816542828046619 64-65 4.389827631727831 66-67 2.4869875155584054 68-69 4.769594161354807 70-71 2.3304605287971936 72-73 1.4620186323690265 74-75 0.7847565345302305 76-77 1.038405687775808 78-79 1.5023290461283143 80-81 2.0447516312752234 82-83 0.6878701014596613 84-85 1.0648078301210726 86-87 0.4127692075585562 88-89 0.6027703390789424 90-91 0.21404593972768077 92-93 0.25388488665937464 94-95 0.5115415079395014 96-97 0.4891468336287859 98-99 0.4643948251801003 100 0.27580809414249613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 212104.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.193236366304774 #Duplication Level Percentage of deduplicated Percentage of total 1 92.99424952868097 57.836133416443815 2 3.6376472871010384 4.524741150878444 3 1.048636493173949 1.9565429184690102 4 0.5314794019390876 1.322176962744799 5 0.3307536817611935 1.0285320954399728 6 0.2532175765671246 0.9449052354925236 7 0.1514179138454536 0.6592019074118148 8 0.1304668018859287 0.6491322118117937 9 0.1224898443375646 0.6856235857222232 >10 0.6711812448770174 8.11509055924464 >50 0.0697070992879763 3.0477230020058896 >100 0.04481170668512762 6.8535059419217985 >500 0.005974894224683683 2.746728614894558 >1k 0.007966525632911578 9.629962397518712 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3956 1.8651227699619057 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2959 1.395070342850677 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2062 0.9721645984988496 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1569 0.7397314524950025 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1494 0.7043714404254516 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1260 0.5940482027684533 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1142 0.53841511711236 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1107 0.5219137781465697 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 911 0.42950627993814355 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 861 0.40593293855844303 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 853 0.40216120393769095 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 697 0.3286123788330253 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 558 0.26307848979745785 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 555 0.26166408931467583 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 496 0.23384754648662923 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 494 0.23290461283144115 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 461 0.21734620752083883 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 448 0.21121713876211667 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 443 0.20885980462414666 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 428 0.20178780221023648 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 424 0.19990193489986047 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 418 0.19707313393429637 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 370 0.17444272620978388 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 370 0.17444272620978388 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 363 0.17114245841662581 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 351 0.16548485648549768 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 343 0.1617131218647456 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 331 0.15605551993361746 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 270 0.12729604345038284 No Hit CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 259 0.1221099083468487 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 259 0.1221099083468487 No Hit GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 255 0.12022404103647269 No Hit ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 230 0.1084373703466224 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 230 0.1084373703466224 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 227 0.10702296986384038 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 219 0.10325123524308831 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 217 0.10230830158790027 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 216 0.10183683476030626 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0011786670689850262 0.0 24-25 0.0 0.0 0.0 0.0030645343793610684 0.0 26-27 0.0 0.0 0.0 0.005893335344925131 0.0 28-29 0.0 0.0 0.0 0.013672538000226305 0.0 30-31 0.0 0.0 0.0 0.030409610379813677 0.0 32-33 0.0 0.0 0.0 0.0669482895183495 0.0 34-35 0.0 0.0 0.0 0.1313035114849319 0.0 36-37 0.0 0.0 0.0 0.19660166710670238 0.0 38-39 0.0 0.0 0.0 0.2866518311771584 0.0 40-41 0.0 0.0 0.0 0.47288122807679256 0.0 42-43 0.0 0.0 0.0 0.5883906008373251 0.0 44-45 0.0 0.0 0.0 0.6895202353562403 0.0 46-47 0.0 0.0 0.0 0.8472258891864368 0.0 48-49 0.0 0.0 0.0 0.9768792667747896 0.0 50-51 0.0 0.0 0.0 1.1018179760872024 0.0 52-53 0.0 0.0 0.0 1.2922905744351827 0.0 54-55 0.0 0.0 0.0 1.4705050352657187 4.714668275940105E-4 56-57 0.0 0.0 0.0 1.6343397578546373 4.714668275940105E-4 58-59 0.0 0.0 0.0 1.8356560932372799 4.714668275940105E-4 60-61 0.0 0.0 0.0 2.0155206879643948 4.714668275940105E-4 62-63 0.0 0.0 0.0 2.210472221174518 4.714668275940105E-4 64-65 0.0 0.0 0.0 2.474493644627164 4.714668275940105E-4 66-67 0.0 0.0 0.0 2.745351337079923 4.714668275940105E-4 68-69 0.0 0.0 0.0 3.0786783841888887 4.714668275940105E-4 70-71 0.0 0.0 0.0 3.6774412552332816 4.714668275940105E-4 72-73 0.0 0.0 0.0 4.087853128653868 4.714668275940105E-4 74-75 0.0 0.0 0.0 4.449703918832271 4.714668275940105E-4 76-77 0.0 0.0 0.0 4.893354203598236 4.714668275940105E-4 78-79 0.0 0.0 0.0 5.30848074529476 4.714668275940105E-4 80-81 0.0 0.0 0.0 5.7634462339229815 4.714668275940105E-4 82-83 0.0 0.0 0.0 6.426564326933956 4.714668275940105E-4 84-85 0.0 0.0 0.0 6.999867989288274 4.714668275940105E-4 86-87 0.0 0.0 0.0 7.519188699883076 4.714668275940105E-4 88 0.0 0.0 0.0 8.030965941236374 4.714668275940105E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGA 25 0.004097625 59.982258 6 TGTACTG 70 8.112693E-10 57.17381 5 TACATAA 35 0.0065750806 53.22156 2 CTGTGCG 85 7.6397555E-11 52.969845 9 GAACAAA 235 0.0 47.559692 1 TACTGTG 75 9.536416E-8 46.355473 7 ACTGTGC 85 2.4089604E-7 41.19877 8 GTACTGT 100 1.9177605E-8 39.98817 6 AAAAGTA 145 7.470226E-8 34.257557 2 GTACATA 150 1.0062831E-7 33.11564 1 ACATAGA 85 2.3887551E-4 33.043888 3 AAAAAGT 135 1.657585E-6 32.195763 1 GTACAAA 100 3.4425495E-4 31.04591 1 AAGTACT 170 2.1809683E-8 29.739498 4 GTGCGTT 60 3.574538E-6 29.19469 10-11 GAACATA 110 6.0154963E-4 28.223555 1 CATAGAA 80 0.0070835827 28.087301 4 GTACATT 210 6.409573E-8 26.61078 1 AGTACTC 170 6.729897E-8 26.484922 5 TACATTG 215 8.051211E-8 25.991926 2 >>END_MODULE