FastQCFastQC Report
Wed 25 May 2016
SRR1295076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916786
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT750.0087528126.9104889
GTGCGCA1904.896783E-621.2451219
TCGACGC1300.00546119419.3303325
GTATTAG12350.019.1380561
CGCCTAG2501.93678E-417.415631
GTACATG103400.017.3241961
TACATGG106800.016.3026182
ACATGGG106800.015.4667423
TCTATAC12050.015.4393633
GTATAGA12500.015.4252731
GTATATA20300.015.3198721
GTATAAC7351.8189894E-1215.2323291
CTAATAC13000.015.178443
TAGGGCG2751.2137539E-414.620765
GTGTAAG11100.014.5690591
GTATAAT16300.014.5003061
GTACAAG11700.014.3535411
ATAGTAC11200.014.0942673
GTGTATA17550.013.821931
AGTACCG2200.00683077813.7069615
Produced by FastQC (version 0.11.5)