##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295076_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2916786 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.177680501757756 33.0 27.0 33.0 6.0 33.0 2 22.2427421826627 27.0 2.0 33.0 2.0 33.0 3 24.70841604423499 27.0 15.0 33.0 15.0 33.0 4 24.5934751469597 33.0 6.0 37.0 2.0 37.0 5 26.805032319820516 33.0 15.0 37.0 2.0 37.0 6 27.700762071677524 33.0 15.0 37.0 2.0 37.0 7 27.36859509062372 33.0 15.0 37.0 6.0 37.0 8 28.067563749963146 33.0 22.0 37.0 6.0 37.0 9 29.112359631457366 33.0 27.0 37.0 15.0 37.0 10-11 28.689956513779208 33.0 24.5 37.0 10.5 37.0 12-13 27.50834891555294 33.0 15.0 37.0 4.0 37.0 14-15 29.26606751403771 33.0 22.0 37.0 6.0 40.0 16-17 29.69142302520651 35.0 27.0 38.5 6.0 40.0 18-19 29.889776966839527 35.0 27.0 40.0 10.5 40.0 20-21 29.39639743882479 33.0 24.5 40.0 6.0 40.0 22-23 30.175840119912806 33.0 27.0 40.0 15.0 40.0 24-25 30.367422052903436 33.0 27.0 40.0 15.0 40.0 26-27 28.87293839863466 33.0 18.5 38.5 4.0 40.0 28-29 29.60619856924711 33.0 22.0 40.0 10.5 40.0 30-31 30.32305318250979 33.0 27.0 40.0 15.0 40.0 32-33 29.129200428142482 33.0 22.0 37.0 6.0 40.0 34-35 28.727012711937043 33.0 22.0 37.0 6.0 40.0 36-37 29.031127926423125 33.0 22.0 37.0 6.0 40.0 38-39 29.174103276688793 33.0 24.5 37.0 6.0 40.0 40-41 28.20013861147167 33.0 22.0 37.0 6.0 40.0 42-43 28.863479185651606 33.0 22.0 37.0 6.0 40.0 44-45 28.730701532440158 33.0 22.0 37.0 6.0 40.0 46-47 28.47321641011716 33.0 22.0 37.0 6.0 40.0 48-49 28.433685913193493 33.0 22.0 37.0 6.0 40.0 50-51 28.297078873801507 33.0 22.0 37.0 6.0 40.0 52-53 28.55141241078365 33.0 22.0 37.0 6.0 40.0 54-55 28.569513498761992 33.0 22.0 37.0 6.0 40.0 56-57 28.556541000951047 33.0 22.0 37.0 6.0 40.0 58-59 28.436237351660353 33.0 22.0 37.0 6.0 40.0 60-61 28.400999421966507 33.0 22.0 37.0 6.0 40.0 62-63 28.37842988824 33.0 22.0 37.0 6.0 40.0 64-65 27.96204983841804 33.0 22.0 37.0 6.0 40.0 66-67 28.18420326345505 33.0 22.0 37.0 10.5 40.0 68-69 27.70671862796928 33.0 22.0 37.0 6.0 38.5 70-71 27.864989409576157 33.0 22.0 37.0 10.5 37.0 72-73 27.915626480653707 33.0 22.0 37.0 10.5 37.0 74-75 28.026818559880635 33.0 22.0 37.0 15.0 37.0 76-77 27.810840596464736 33.0 22.0 37.0 10.5 37.0 78-79 27.35373592714721 33.0 22.0 37.0 10.5 37.0 80-81 27.18407178312019 27.0 22.0 35.0 6.0 37.0 82-83 27.259068371831184 27.0 22.0 33.0 15.0 37.0 84-85 27.208339418798637 27.0 22.0 33.0 10.5 37.0 86-87 27.0062287737256 27.0 22.0 33.0 15.0 37.0 88-89 26.94978239747448 27.0 22.0 33.0 15.0 37.0 90-91 27.0835469245944 27.0 22.0 33.0 15.0 37.0 92-93 26.987227036882377 27.0 22.0 33.0 15.0 37.0 94-95 26.857913299090164 27.0 22.0 33.0 15.0 37.0 96-97 26.945412519122073 27.0 22.0 33.0 15.0 37.0 98-99 27.008147152379365 27.0 22.0 33.0 15.0 37.0 100 26.65092536785352 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.2893037558824325 1101 2 11.19744749140887 1101 3 8.444136071829636 1101 4 12.76940639329123 1101 5 10.831958788391027 1101 6 10.013129666777235 1101 7 9.440973375234112 1101 8 9.174644551590564 1101 9 7.464694036411483 1101 10-11 7.794706283536744 1101 12-13 9.240076763907819 1101 14-15 8.093492353482656 1101 16-17 8.351408921063225 1101 18-19 8.276270663244187 1101 20-21 7.347714141201902 1101 22-23 7.413895748351042 1101 24-25 7.363885811270887 1101 26-27 8.630441596976215 1101 28-29 8.370265043906599 1101 30-31 5.622864472886846 1101 32-33 7.057686330777312 1101 34-35 8.790913705458195 1101 36-37 8.346253295049213 1101 38-39 8.309015914586649 1101 40-41 9.233956288508672 1101 42-43 8.732852685503833 1101 44-45 8.58026026812652 1101 46-47 7.435461307569049 1101 48-49 5.500413191923091 1101 50-51 6.960043560954119 1101 52-53 6.10398489920863 1101 54-55 4.193168193923988 1101 56-57 6.4578178767634355 1101 58-59 6.415901204420269 1101 60-61 6.9451296992465394 1101 62-63 6.483801819693198 1101 64-65 6.9497559020841955 1101 66-67 5.920825960603391 1101 68-69 6.938591299110865 1101 70-71 6.4569424694376565 1101 72-73 5.654090036190524 1101 74-75 5.073531426062047 1101 76-77 5.187457952114837 1101 78-79 5.311284117824368 1101 80-81 5.009163533373144 1101 82-83 4.598600863496813 1101 84-85 4.323781395270306 1101 86-87 4.296055442532687 1101 88-89 4.117087219470523 1101 90-91 3.936798342459049 1101 92-93 3.9315591190591377 1101 94-95 3.829422921807616 1101 96-97 3.953693406750453 1101 98-99 3.6663003178405553 1101 100 3.839466672748557 1102 1 6.833418853927295 1102 2 10.598203898519792 1102 3 8.280121506895338 1102 4 12.63803452011334 1102 5 10.7265921966532 1102 6 9.63512328375413 1102 7 6.903320318266669 1102 8 7.9242732763865185 1102 9 6.688506134554682 1102 10-11 7.548019525478747 1102 12-13 9.203940519243144 1102 14-15 6.460762982835231 1102 16-17 7.583458357209651 1102 18-19 7.5425528105464 1102 20-21 7.2086530892645975 1102 22-23 6.787485145937101 1102 24-25 7.068869592968721 1102 26-27 8.605412853009344 1102 28-29 7.872536177365035 1102 30-31 4.429962084666734 1102 32-33 4.542198646853169 1102 34-35 5.288122126161596 1102 36-37 5.381276397003752 1102 38-39 4.877010531828752 1102 40-41 6.509133631955244 1102 42-43 7.162314307900235 1102 44-45 6.79216842798618 1102 46-47 5.59651353045237 1102 48-49 3.926193215027528 1102 50-51 5.273514511047146 1102 52-53 6.13907670110645 1102 54-55 5.600974782165359 1102 56-57 6.525668414095584 1102 58-59 6.223225254093823 1102 60-61 6.14357203698653 1102 62-63 4.486950954071432 1102 64-65 6.289858466517867 1102 66-67 4.0855341553283075 1102 68-69 6.316496438805302 1102 70-71 5.525922030056016 1102 72-73 5.25979862526032 1102 74-75 4.529194086866848 1102 76-77 3.6110232246349625 1102 78-79 4.643379507068591 1102 80-81 4.644928626997771 1102 82-83 4.0224034258445265 1102 84-85 3.1488124735204437 1102 86-87 3.4564858645015732 1102 88-89 3.5930348143144393 1102 90-91 3.4685965824094644 1102 92-93 3.3344374765779605 1102 94-95 3.2684225746450615 1102 96-97 3.5162225232093327 1102 98-99 3.30011535370409 1102 100 3.289557088976096 1103 1 6.695376241433738 1103 2 10.840910801087638 1103 3 8.018330737323971 1103 4 11.882572649542755 1103 5 10.454574370502272 1103 6 9.305996265519948 1103 7 8.214396915735495 1103 8 8.217974761016155 1103 9 7.274565906686629 1103 10-11 7.557099484012408 1103 12-13 8.919369216130843 1103 14-15 8.775419841692507 1103 16-17 8.18901901281997 1103 18-19 7.371114705491696 1103 20-21 8.441717178967021 1103 22-23 7.111849291733751 1103 24-25 5.437190398217023 1103 26-27 6.219064520518206 1103 28-29 7.2115427320762535 1103 30-31 6.334140443154773 1103 32-33 8.173856872871841 1103 34-35 7.522877098804223 1103 36-37 4.399550985634814 1103 38-39 6.736253048393859 1103 40-41 7.139686914092241 1103 42-43 7.698632714376462 1103 44-45 5.944167982148851 1103 46-47 6.540616535089534 1103 48-49 5.855723587555364 1103 50-51 6.728997215233086 1103 52-53 6.457711334993267 1103 54-55 5.269441891808938 1103 56-57 6.177447280309941 1103 58-59 5.676842072460662 1103 60-61 5.300573417914638 1103 62-63 2.8501239034931416 1103 64-65 5.297059167681709 1103 66-67 4.409795917621192 1103 68-69 5.452734605779593 1103 70-71 4.01930972811207 1103 72-73 4.736521916683973 1103 74-75 3.554388344651329 1103 76-77 3.786021833305348 1103 78-79 3.2016494491813745 1103 80-81 4.378552702968165 1103 82-83 3.6556446318701923 1103 84-85 3.5376237844705933 1103 86-87 3.0137161653584066 1103 88-89 3.1546940685021134 1103 90-91 2.876382251314215 1103 92-93 2.5797671916130085 1103 94-95 3.063739951907035 1103 96-97 3.2934613216930266 1103 98-99 3.05002189943891 1103 100 3.260777738709052 1104 1 6.352534675096887 1104 2 9.599599293455142 1104 3 7.432553426952353 1104 4 12.189198750926561 1104 5 9.680369309205517 1104 6 8.403139516221447 1104 7 4.121686052875869 1104 8 6.802481794550225 1104 9 6.689163372478536 1104 10-11 7.22806826066191 1104 12-13 7.485610844994877 1104 14-15 7.363728187368768 1104 16-17 7.261975465114361 1104 18-19 6.955699898002674 1104 20-21 7.626114209544976 1104 22-23 6.5322282776481195 1104 24-25 6.0372694471228705 1104 26-27 8.324750276547665 1104 28-29 7.310841738915549 1104 30-31 6.336911991429936 1104 32-33 7.729115153467209 1104 34-35 8.104213470277593 1104 36-37 6.820220862236486 1104 38-39 7.40852293985348 1104 40-41 7.351426237653378 1104 42-43 7.364990266158717 1104 44-45 6.901860953567432 1104 46-47 6.652527731014743 1104 48-49 5.646290998512168 1104 50-51 5.900955941102467 1104 52-53 6.171709878605196 1104 54-55 4.896670776890478 1104 56-57 6.151821776339311 1104 58-59 4.0457714028162215 1104 60-61 4.420226686120646 1104 62-63 3.8946093233616175 1104 64-65 3.101271007915475 1104 66-67 3.5412876784268654 1104 68-69 4.472112073397785 1104 70-71 2.959416891034074 1104 72-73 2.8165832521345635 1104 74-75 3.3308066979268105 1104 76-77 3.9481232418694034 1104 78-79 4.445717724530031 1104 80-81 3.206314229869559 1104 82-83 3.074510932040905 1104 84-85 3.140823195105476 1104 86-87 1.012999501709757 1104 88-89 0.6547899873653584 1104 90-91 2.2890991424751306 1104 92-93 2.584610687934891 1104 94-95 2.5159153638667533 1104 96-97 2.9597930268433146 1104 98-99 2.6265073069257667 1104 100 0.8962472110508344 1105 1 5.84937595336201 1105 2 -2.8132712879244757 1105 3 0.5038517755303822 1105 4 8.51249920150357 1105 5 8.955467905444813 1105 6 8.259578709137084 1105 7 5.473138201181264 1105 8 6.263840393495062 1105 9 5.1862179724223445 1105 10-11 5.12882633306641 1105 12-13 5.2804664106095025 1105 14-15 6.105539496539517 1105 16-17 6.307911687135402 1105 18-19 6.392936497759177 1105 20-21 7.294011898386696 1105 22-23 2.1065342017472197 1105 24-25 4.235937747521415 1105 26-27 7.149766405747759 1105 28-29 6.210945277974226 1105 30-31 5.52470036591874 1105 32-33 7.375547218317006 1105 34-35 6.37536977783542 1105 36-37 6.450400156505939 1105 38-39 4.3481203862369 1105 40-41 5.702347423097557 1105 42-43 5.896830087592864 1105 44-45 6.4530826104004895 1105 46-47 5.712330902743901 1105 48-49 5.278056937803939 1105 50-51 6.226339920124097 1105 52-53 5.916771741892017 1105 54-55 5.688915233128316 1105 56-57 5.500402406730885 1105 58-59 2.2519088236010383 1105 60-61 4.775238444827814 1105 62-63 -0.5741910803886796 1105 64-65 -0.19159338193249553 1105 66-67 1.1475510110317835 1105 68-69 3.0608611758858437 1105 70-71 3.377616612155002 1105 72-73 3.066936793794728 1105 74-75 2.9464051953097155 1105 76-77 2.006491174938425 1105 78-79 3.444103763926904 1105 80-81 1.9921007625077713 1105 82-83 1.2701797215184207 1105 84-85 1.538869220558393 1105 86-87 -0.022571107765887888 1105 88-89 1.7317364176091665 1105 90-91 1.6211343353491472 1105 92-93 2.0167488995708105 1105 94-95 1.3813739014165698 1105 96-97 2.375547873407797 1105 98-99 2.230570304459558 1105 100 2.2974797941162137 1106 1 6.086963299279454 1106 2 10.302866000944942 1106 3 6.8892909575891785 1106 4 11.939128011607185 1106 5 10.061916318911138 1106 6 9.45266062349435 1106 7 6.7524902997280485 1106 8 7.601704129477529 1106 9 6.986774751848518 1106 10-11 5.938124735257301 1106 12-13 8.081219826395422 1106 14-15 8.031886923908427 1106 16-17 7.8914185254157445 1106 18-19 7.295549818459886 1106 20-21 8.393581329005617 1106 22-23 6.279940624152363 1106 24-25 5.1416951342213615 1106 26-27 7.869001265573726 1106 28-29 6.58110672310827 1106 30-31 5.596203149170826 1106 32-33 7.561957713295694 1106 34-35 7.029595447020874 1106 36-37 6.089397060792724 1106 38-39 7.293937006382716 1106 40-41 7.012695369600905 1106 42-43 6.364977138873133 1106 44-45 4.773272179616949 1106 46-47 6.176236855296246 1106 48-49 6.095919032784899 1106 50-51 6.756885189318012 1106 52-53 5.8543993516907555 1106 54-55 5.515005660567926 1106 56-57 5.180335448193006 1106 58-59 5.1893796222888575 1106 60-61 5.06286964109211 1106 62-63 5.061304620755013 1106 64-65 5.238969845391107 1106 66-67 3.1900914593967435 1106 68-69 3.5390073963445907 1106 70-71 3.0141304824880137 1106 72-73 2.9042002096270885 1106 74-75 3.0308313943631973 1106 76-77 3.6481291627816006 1106 78-79 4.3712995710132425 1106 80-81 3.8694951152952335 1106 82-83 2.3144024157435936 1106 84-85 2.7572116568057403 1106 86-87 3.3687541609223075 1106 88-89 3.3817497313949723 1106 90-91 3.276790539857153 1106 92-93 3.114432881383557 1106 94-95 1.6863926439243428 1106 96-97 1.736037820725457 1106 98-99 2.0793032629427373 1106 100 3.0971619919848727 1107 1 6.272042519605474 1107 2 10.461271378094942 1107 3 6.309836491256192 1107 4 11.566963176312473 1107 5 9.609211773653797 1107 6 8.314028110544282 1107 7 3.9996810601370463 1107 8 6.4897490644369356 1107 9 6.082481274842873 1107 10-11 4.8154736278561465 1107 12-13 6.423034981688755 1107 14-15 6.482607604107212 1107 16-17 7.4196236078684805 1107 18-19 6.549600978845479 1107 20-21 5.834192791702694 1107 22-23 6.063879131725248 1107 24-25 4.87137618508768 1107 26-27 7.521915835476367 1107 28-29 6.675987466037654 1107 30-31 5.3229653186158 1107 32-33 5.399026490296915 1107 34-35 7.012072399964396 1107 36-37 6.823577617205476 1107 38-39 6.6792681435064445 1107 40-41 8.1035567446064 1107 42-43 6.499419174926491 1107 44-45 4.42509106503697 1107 46-47 3.782642497548647 1107 48-49 4.395077536082059 1107 50-51 5.188524457564331 1107 52-53 4.199032571735042 1107 54-55 5.517179791182816 1107 56-57 5.489392330706735 1107 58-59 3.2157588171268365 1107 60-61 3.142229238564912 1107 62-63 0.7945582199590682 1107 64-65 2.3896226252855968 1107 66-67 4.428612282255429 1107 68-69 5.311503334131835 1107 70-71 4.563460770645513 1107 72-73 2.6707588038098784 1107 74-75 2.804495935241299 1107 76-77 3.303584387447515 1107 78-79 4.053049433769456 1107 80-81 2.3568612712200867 1107 82-83 1.8806062037365479 1107 84-85 2.59570588703075 1107 86-87 2.530847219021883 1107 88-89 2.8712654413769663 1107 90-91 1.6061644468343808 1107 92-93 2.520150086490549 1107 94-95 2.56642900693058 1107 96-97 2.69950200614079 1107 98-99 2.258856928965674 1107 100 0.34467226051872046 1108 1 4.258949217060827 1108 2 -3.2623507415000184 1108 3 0.056483667872885945 1108 4 6.426315652402394 1108 5 7.032473795040573 1108 6 6.84720940802249 1108 7 4.367050828877339 1108 8 5.25065062257238 1108 9 4.986800619709516 1108 10-11 3.2786062846227857 1108 12-13 2.616113466246066 1108 14-15 4.066421972449316 1108 16-17 4.5727201257715215 1108 18-19 4.001562611418201 1108 20-21 4.711406382212669 1108 22-23 4.151767286217915 1108 24-25 3.1308160532314595 1108 26-27 4.805656078178291 1108 28-29 6.139245352955022 1108 30-31 4.582812283571734 1108 32-33 4.644296071499291 1108 34-35 3.125186836451796 1108 36-37 1.3066792486198757 1108 38-39 2.37167811761552 1108 40-41 5.4322103135330835 1108 42-43 3.1461433003129216 1108 44-45 3.7117737508599085 1108 46-47 4.6420278336424765 1108 48-49 4.831437794752134 1108 50-51 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-17.381415993488304 2213 48-49 -6.299211136181562 2213 50-51 -23.789335321033455 2213 52-53 -18.142330863469628 2213 54-55 -18.070403422215435 2213 56-57 -18.00217320035916 2213 58-59 -18.435709535723028 2213 60-61 -19.75185769082522 2213 62-63 -18.056053632372457 2213 64-65 -18.039389672115018 2213 66-67 -8.958469882353967 2213 68-69 -23.22740248970901 2213 70-71 -17.89707738061513 2213 72-73 -9.768874583126834 2213 74-75 -4.382453533664425 2213 76-77 -3.171823649072735 2213 78-79 -4.724450733380618 2213 80-81 -3.3028445695641686 2213 82-83 -3.375944418096335 2213 84-85 -3.1883326165947103 2213 86-87 -3.419912756653801 2213 88-89 -2.9072942080826785 2213 90-91 -2.8364171490494776 2213 92-93 -3.8801062033702465 2213 94-95 -3.8530603042546865 2213 96-97 -3.465403828497326 2213 98-99 -3.9230166801069117 2213 100 -3.3144804277182196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 818.0 3 841.0 4 4729.0 5 16385.0 6 21329.0 7 25145.0 8 23161.0 9 22941.0 10 26710.0 11 33154.0 12 41429.0 13 56512.0 14 44305.0 15 49344.0 16 56193.0 17 59654.0 18 59820.0 19 59815.0 20 57968.0 21 56680.0 22 58681.0 23 64126.0 24 70144.0 25 77201.0 26 84835.0 27 91438.0 28 99685.0 29 104451.0 30 109535.0 31 118728.0 32 134655.0 33 163993.0 34 201115.0 35 238614.0 36 266721.0 37 237358.0 38 78375.0 39 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.08461410274901 22.26879829740553 14.213756909504086 23.432830690341376 2 14.6481032646616 22.015533766662994 40.982152693621245 22.354210275054164 3 17.015567529169665 27.87823530165635 30.593572695765847 24.512624473408138 4 11.955680477644236 18.477143491558166 40.57074794562356 28.996428085174042 5 12.494800729352507 38.31365145662137 36.77592539154734 12.415622422478776 6 28.618220080753247 40.1955973737 19.008422745281 12.177759800265756 7 25.47213541501039 32.755024581832004 25.050834128659076 16.72200587449853 8 23.04184528026542 36.955966107560826 22.689433281975415 17.312755330198343 9 24.117580933207073 17.517814273173652 23.06391310469121 35.30069168892807 10-11 22.391023106888568 28.137962102260328 30.039508556866814 19.431506233984294 12-13 23.21807454625452 25.841473846875562 30.04966709745467 20.890784509415248 14-15 20.81020654001836 27.2924133449986 27.29594865098306 24.60143146399998 16-17 20.272470715786042 31.025282787835096 28.161087353854843 20.54115914252402 18-19 20.241293923563983 30.09074672867214 29.69224269796013 19.97571664980374 20-21 21.776930152507102 29.425173311173243 28.563409178923997 20.23448735739566 22-23 20.698052924002496 29.47938789839017 29.126309810218476 20.696249367388855 24-25 20.95892841623454 29.221026825806078 28.640476983690142 21.17956777426924 26-27 20.958087260277345 29.226237352549372 28.72863054798575 21.087044839187534 28-29 20.698016829515478 29.145173615757574 29.251200187382143 20.90560936734481 30-31 21.396142021560042 29.461436626958353 28.525020684588586 20.61740066689302 32-33 20.437630818984758 29.344565105621324 29.183580303505696 21.03422377188823 34-35 20.577073236777338 28.80065434029034 29.0227739354451 21.599498487487224 36-37 21.21090475000694 29.43081350693577 28.54138789409881 20.81689384895848 38-39 20.880557873602026 29.036385997661068 28.91037863893382 21.17267748980309 40-41 21.11511457242744 29.138596462786726 28.47661782963301 21.269671135152823 42-43 20.755433315334773 29.265365292515728 29.0723270670955 20.906874325053995 44-45 21.23019270629189 28.711731097370652 28.87693787346029 21.181138322877167 46-47 21.056358275059935 29.246345358315818 29.000091936073176 20.697204430551068 48-49 20.842075272249804 28.88872246088157 29.133204806424963 21.135997460443654 50-51 21.257030041992834 28.96741059212074 28.737947510250777 21.03761185563565 52-53 20.580838056266572 29.041938783878862 29.03886330183752 21.338359858017046 54-55 21.02413914712496 29.025567851270456 28.65904751253047 21.29124548907411 56-57 21.21549374188466 28.99228597389918 28.60778476883638 21.184435515379775 58-59 20.81505654981892 28.556071078969975 29.09677610381308 21.532096267398025 60-61 21.33044574631489 28.97118157581421 28.5433820597221 21.1549906181488 62-63 20.73564729037738 29.12669500781937 29.02950612112957 21.10815158067368 64-65 20.77132226802787 28.576325844268418 29.018673488609632 21.633678399094077 66-67 20.978980289219887 28.895057547419732 28.712341655162692 21.413620508197692 68-69 21.416003988406334 28.68885858719269 28.66574527900702 21.22939214539396 70-71 21.43289513334006 28.820741665948983 28.42940675776563 21.316956442945326 72-73 21.058456637836436 28.99510167005256 28.86573815981017 21.08070353230083 74-75 21.374494951940033 28.492145387446726 28.519688747923443 21.613670912689795 76-77 21.315631736228276 28.970945034545874 28.579503216040735 21.13392001318512 78-79 21.236828563733106 28.67466609188556 28.811502655509674 21.27700268887166 80-81 21.426280074028377 28.64114175209244 28.520718757793322 21.41185941608586 82-83 20.79147987417226 28.95463923163262 28.930031595374317 21.323849298820807 84-85 21.089498679583507 28.4729716416689 28.533106670088394 21.9044230086592 86-87 21.31384860562159 28.758826563747192 28.57873126130761 21.348593569323608 88-89 21.098610998855335 28.37805257991402 28.906397335704177 21.616939085526464 90-91 21.469937237633754 28.43523381651635 28.522176832979802 21.572652112870095 92-93 20.904806601104763 28.76065073973595 28.87110636186171 21.46343629729758 94-95 20.945711801354086 28.253404838815122 28.78355528010693 22.017328079723864 96-97 21.222326979977627 28.50394140755218 28.682037256471993 21.5916943559982 98-99 21.149438948328473 28.304718793788425 28.74828937158251 21.79755288630059 100 21.271488411405034 28.21402424999405 28.43986141481173 22.07462592378918 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 331.0 1 412.0 2 409.5 3 350.5 4 427.5 5 612.0 6 889.0 7 1274.5 8 1902.0 9 2797.0 10 3859.0 11 4847.0 12 5799.5 13 6801.5 14 7687.0 15 8596.0 16 9353.0 17 10108.5 18 11202.0 19 12129.0 20 13109.0 21 14460.5 22 16106.5 23 18395.5 24 21278.5 25 24982.5 26 30113.0 27 36961.0 28 45369.0 29 54507.0 30 64071.0 31 73910.5 32 83006.0 33 91097.5 34 98698.5 35 105539.5 36 110934.5 37 114679.5 38 117539.5 39 119015.0 40 118405.5 41 115800.5 42 111404.5 43 106434.5 44 100825.0 45 94716.0 46 89086.0 47 83886.5 48 79492.0 49 76546.0 50 74506.0 51 69506.5 52 61412.5 53 54188.5 54 48696.5 55 44110.5 56 39755.0 57 35346.5 58 30975.5 59 26750.0 60 22646.0 61 18898.5 62 15515.5 63 12493.0 64 9790.0 65 7709.0 66 6008.5 67 4539.5 68 3523.5 69 2710.5 70 2020.5 71 1490.5 72 1130.0 73 870.0 74 644.0 75 470.0 76 311.0 77 214.0 78 164.0 79 114.5 80 73.0 81 59.0 82 46.0 83 31.5 84 17.5 85 8.5 86 7.5 87 8.5 88 7.5 89 6.0 90 3.5 91 3.0 92 3.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.241948500849908 2 25.312312936225005 3 6.36937368733942 4 24.960487330918347 5 12.793533704563858 6 10.495010604137569 7 8.361155052170437 8 9.457087355740187 9 5.7600043335369815 10-11 7.074927677244748 12-13 10.546334218554259 14-15 7.871917926100852 16-17 8.358566586647083 18-19 6.917099848943323 20-21 8.328019265040357 22-23 5.904067010744018 24-25 5.32318791985425 26-27 10.16682403165676 28-29 6.61483221600762 30-31 4.42550464792412 32-33 8.978272660387152 34-35 7.5748100820560715 36-37 7.37836097677375 38-39 6.349437360162864 40-41 9.063366321697924 42-43 6.536029725869502 44-45 6.801133165065933 46-47 6.211957270776807 48-49 4.095158163814555 50-51 7.163466911868063 52-53 4.6878310578835745 54-55 4.972716544854508 56-57 5.011800660041566 58-59 3.89449208820942 60-61 4.3115607384292165 62-63 3.1674932614185614 64-65 4.540528513233402 66-67 2.484909760263523 68-69 5.512848731446188 70-71 2.666822317441184 72-73 1.7559567277133121 74-75 0.8564906715816657 76-77 1.191328400506585 78-79 1.859821049607342 80-81 2.049293297485657 82-83 0.9407272251032472 84-85 1.3403623028909217 86-87 0.43774209009505666 88-89 0.7409525415988694 90-91 0.33288352316556646 92-93 0.41139459665535966 94-95 0.6198774952979067 96-97 0.6001297318349719 98-99 0.4792432492476308 100 0.5797134242964688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2916786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.8513141115288 #Duplication Level Percentage of deduplicated Percentage of total 1 97.26849973005677 93.23313520782827 2 2.253369741447447 4.319769017937873 3 0.28969331665243564 0.8330245527138955 4 0.08460152471169116 0.32436669277818303 5 0.034648774372566575 0.16605652779821842 6 0.018827552732156815 0.1082787402528801 7 0.011325648770429575 0.07599048224679064 8 0.008617793637065574 0.06608214758837652 9 0.006337383282327557 0.054670186407557705 >10 0.022419547852578957 0.3408002158523669 >50 3.98220596192659E-4 0.026256319381080334 >100 0.001045293796856646 0.22369535225950374 >500 1.4364806102686557E-4 0.09338726185055556 >1k 7.182403040630094E-5 0.13448729510446483 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.428431156759529E-5 0.0 4 0.0 0.0 0.0 6.856862313519058E-5 0.0 5 0.0 3.428431156759529E-5 0.0 1.0285293470278587E-4 0.0 6 0.0 3.428431156759529E-5 0.0 1.0285293470278587E-4 0.0 7 3.428431156759529E-5 3.428431156759529E-5 0.0 1.3713724627038116E-4 0.0 8 3.428431156759529E-5 3.428431156759529E-5 0.0 1.7142155783797647E-4 0.0 9 3.428431156759529E-5 3.428431156759529E-5 0.0 2.0570586940557174E-4 0.0 10-11 3.428431156759529E-5 3.428431156759529E-5 0.0 2.3999018097316705E-4 0.0 12-13 5.1426467351392936E-5 6.856862313519058E-5 0.0 2.571323367569647E-4 0.0 14-15 6.856862313519058E-5 8.571077891898822E-5 0.0 2.742744925407623E-4 0.0 16-17 6.856862313519058E-5 1.0285293470278587E-4 0.0 3.257009598921553E-4 0.0 18-19 6.856862313519058E-5 1.0285293470278587E-4 0.0 3.5998527145975057E-4 3.428431156759529E-5 20-21 6.856862313519058E-5 1.0285293470278587E-4 0.0 4.456960503787388E-4 3.428431156759529E-5 22-23 6.856862313519058E-5 1.0285293470278587E-4 0.0 4.971225177301318E-4 3.428431156759529E-5 24-25 1.0285293470278587E-4 1.0285293470278587E-4 0.0 5.999754524329175E-4 3.428431156759529E-5 26-27 1.0285293470278587E-4 1.0285293470278587E-4 0.0 7.199705429195011E-4 3.428431156759529E-5 28-29 1.0285293470278587E-4 1.0285293470278587E-4 0.0 0.0011485244375144424 3.428431156759529E-5 30-31 1.0285293470278587E-4 1.0285293470278587E-4 0.0 0.002862740015894207 3.428431156759529E-5 32-33 1.0285293470278587E-4 1.0285293470278587E-4 0.0 0.007422553454384381 3.428431156759529E-5 34-35 1.0285293470278587E-4 1.0285293470278587E-4 0.0 0.014570832416227999 3.428431156759529E-5 36-37 1.0285293470278587E-4 1.1999509048658352E-4 0.0 0.026244640504994195 3.428431156759529E-5 38-39 1.0285293470278587E-4 1.3713724627038116E-4 0.0 0.044432467791603494 3.428431156759529E-5 40-41 1.0285293470278587E-4 1.3713724627038116E-4 0.0 0.06635728503908069 3.428431156759529E-5 42-43 1.0285293470278587E-4 1.3713724627038116E-4 0.0 0.08816210719607129 6.856862313519058E-5 44-45 1.0285293470278587E-4 1.3713724627038116E-4 0.0 0.11488672806301181 6.856862313519058E-5 46-47 1.0285293470278587E-4 1.3713724627038116E-4 0.0 0.14802251519309267 6.856862313519058E-5 48-49 1.0285293470278587E-4 1.542794020541788E-4 0.0 0.18383247862544594 8.571077891898822E-5 50-51 1.0285293470278587E-4 1.7142155783797647E-4 0.0 0.2228651673451532 1.0285293470278587E-4 52-53 1.0285293470278587E-4 1.7142155783797647E-4 0.0 0.26539485584475514 1.0285293470278587E-4 54-55 1.0285293470278587E-4 1.7142155783797647E-4 0.0 0.31289576952165843 1.0285293470278587E-4 56-57 1.0285293470278587E-4 1.7142155783797647E-4 3.428431156759529E-5 0.3702362806184616 1.0285293470278587E-4 58-59 1.0285293470278587E-4 1.7142155783797647E-4 3.428431156759529E-5 0.43230802671159285 1.0285293470278587E-4 60-61 1.0285293470278587E-4 1.7142155783797647E-4 3.428431156759529E-5 0.4986310274391059 1.0285293470278587E-4 62-63 1.0285293470278587E-4 1.7142155783797647E-4 3.428431156759529E-5 0.5663425427851065 1.0285293470278587E-4 64-65 1.0285293470278587E-4 1.7142155783797647E-4 3.428431156759529E-5 0.6404137979268962 1.0285293470278587E-4 66-67 1.0285293470278587E-4 1.885637136217741E-4 3.428431156759529E-5 0.7304272579476176 1.0285293470278587E-4 68-69 1.0285293470278587E-4 2.0570586940557174E-4 3.428431156759529E-5 0.833503040675593 1.0285293470278587E-4 70-71 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 0.9542009595493122 1.0285293470278587E-4 72-73 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 1.0798015349771974 1.1999509048658352E-4 74-75 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 1.2142988892568738 1.3713724627038116E-4 76-77 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 1.3649612964406712 1.3713724627038116E-4 78-79 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 1.5348057759465386 1.3713724627038116E-4 80-81 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 1.7264207932978284 1.3713724627038116E-4 82-83 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 1.9344237115784293 1.3713724627038116E-4 84-85 1.3713724627038116E-4 2.0570586940557174E-4 3.428431156759529E-5 2.149763472534495 1.3713724627038116E-4 86-87 1.7142155783797647E-4 2.0570586940557174E-4 3.428431156759529E-5 2.3835139088023602 1.3713724627038116E-4 88 1.7142155783797647E-4 2.0570586940557174E-4 3.428431156759529E-5 2.5800658670193837 1.3713724627038116E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGTT 75 0.00875281 26.910488 9 GTGCGCA 190 4.896783E-6 21.245121 9 TCGACGC 130 0.005461194 19.330332 5 GTATTAG 1235 0.0 19.138056 1 CGCCTAG 250 1.93678E-4 17.41563 1 GTACATG 10340 0.0 17.324196 1 TACATGG 10680 0.0 16.302618 2 ACATGGG 10680 0.0 15.466742 3 TCTATAC 1205 0.0 15.439363 3 GTATAGA 1250 0.0 15.425273 1 GTATATA 2030 0.0 15.319872 1 GTATAAC 735 1.8189894E-12 15.232329 1 CTAATAC 1300 0.0 15.17844 3 TAGGGCG 275 1.2137539E-4 14.62076 5 GTGTAAG 1110 0.0 14.569059 1 GTATAAT 1630 0.0 14.500306 1 GTACAAG 1170 0.0 14.353541 1 ATAGTAC 1120 0.0 14.094267 3 GTGTATA 1755 0.0 13.82193 1 AGTACCG 220 0.006830778 13.706961 5 >>END_MODULE