FastQCFastQC Report
Wed 25 May 2016
SRR1295076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916786
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58880.20186602651000107No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45430.1557536274515854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1805.6393837E-620.90185
GTATCAA111050.019.0372941
TATATCG1851.6429907E-417.7947755
CAACGCA119000.017.3888935
TATCAAC120950.017.3784852
AACGCAG120750.017.1516656
TCAACGC120500.017.1334064
ATCAACG120800.017.090273
GAGTACT41500.016.17934212-13
ACGCAGA130900.015.7111277
TAGACTG15050.015.62426955
CGCAGAG131100.015.6155278
GTATTAG15100.015.1535991
TGGACCG2801.4714968E-515.1164815
GTATATA23900.014.985441
GTACATG134500.014.6456221
GTACAGG14750.014.5014471
CGTACAC2308.6188654E-414.3126983
AGTACTT49350.014.22416812-13
GTATACG2459.08975E-414.212381
Produced by FastQC (version 0.11.5)