##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295076_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2916786 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.259366645341824 33.0 33.0 33.0 27.0 33.0 2 30.020949771426494 33.0 33.0 33.0 27.0 33.0 3 30.813679166040977 33.0 33.0 33.0 27.0 33.0 4 35.055127458785115 37.0 37.0 37.0 33.0 37.0 5 35.37207974805145 37.0 37.0 37.0 33.0 37.0 6 35.089288689674184 37.0 37.0 37.0 33.0 37.0 7 34.9916126860181 37.0 37.0 37.0 33.0 37.0 8 34.98278070451518 37.0 37.0 37.0 33.0 37.0 9 34.95659846145723 37.0 37.0 37.0 33.0 37.0 10-11 34.93132338128338 37.0 37.0 37.0 33.0 37.0 12-13 34.84655027828576 37.0 37.0 37.0 30.0 37.0 14-15 36.27010774873439 37.0 37.0 40.0 27.0 40.0 16-17 36.25508230634678 37.0 37.0 40.0 27.0 40.0 18-19 36.29310189365967 38.5 37.0 40.0 27.0 40.0 20-21 36.295687616438094 40.0 37.0 40.0 27.0 40.0 22-23 36.25660247272169 38.5 37.0 40.0 27.0 40.0 24-25 36.2326358532988 37.0 37.0 40.0 27.0 40.0 26-27 36.211732365692924 37.0 37.0 40.0 27.0 40.0 28-29 36.15777451619694 37.0 37.0 40.0 27.0 40.0 30-31 36.06851102549176 37.0 37.0 40.0 27.0 40.0 32-33 36.01973062130715 37.0 37.0 40.0 27.0 40.0 34-35 35.90921771429238 37.0 37.0 40.0 27.0 40.0 36-37 35.911038554079724 37.0 37.0 40.0 27.0 40.0 38-39 35.85535620371189 37.0 37.0 40.0 27.0 40.0 40-41 35.796927337144375 37.0 37.0 40.0 27.0 40.0 42-43 35.71501937406447 37.0 37.0 40.0 27.0 40.0 44-45 35.61515963118309 37.0 37.0 40.0 27.0 40.0 46-47 35.508040185327275 37.0 37.0 40.0 27.0 40.0 48-49 35.40678387101419 37.0 37.0 40.0 27.0 40.0 50-51 35.31065271843735 37.0 33.0 40.0 27.0 40.0 52-53 35.22139831307473 37.0 33.0 40.0 27.0 40.0 54-55 35.114842158457975 37.0 33.0 40.0 27.0 40.0 56-57 34.99272692614405 37.0 33.0 40.0 27.0 40.0 58-59 34.877612207409115 37.0 33.0 40.0 27.0 40.0 60-61 34.7285591058103 37.0 33.0 40.0 27.0 40.0 62-63 34.55135498456177 37.0 33.0 40.0 27.0 40.0 64-65 34.38529343599428 37.0 33.0 40.0 24.5 40.0 66-67 34.16911302371857 37.0 33.0 40.0 22.0 40.0 68-69 33.959543826664 37.0 33.0 38.5 22.0 40.0 70-71 33.73521454779336 37.0 33.0 37.0 22.0 40.0 72-73 33.52343932671098 37.0 33.0 37.0 22.0 40.0 74-75 33.28865230428286 37.0 33.0 37.0 22.0 40.0 76-77 33.04736326216596 37.0 33.0 37.0 22.0 38.5 78-79 32.811415887212846 37.0 33.0 37.0 22.0 37.0 80-81 32.59136923312166 37.0 33.0 37.0 22.0 37.0 82-83 32.375490179944634 37.0 33.0 37.0 22.0 37.0 84-85 32.197764080052494 35.0 33.0 37.0 22.0 37.0 86-87 32.21425414823028 33.0 33.0 37.0 22.0 37.0 88-89 32.216710790575654 33.0 33.0 37.0 22.0 37.0 90-91 32.21526244983348 33.0 33.0 37.0 22.0 37.0 92-93 32.172332149153206 33.0 33.0 37.0 22.0 37.0 94-95 32.19040546683919 33.0 33.0 37.0 22.0 37.0 96-97 32.14174437205884 33.0 33.0 37.0 22.0 37.0 98-99 32.06725621968839 33.0 33.0 37.0 22.0 37.0 100 31.541501844838805 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6950722222615227 1101 2 4.182553913200014 1101 3 2.332993907253659 1101 4 1.2041682250074857 1101 5 0.4987376954124514 1101 6 0.5678815017169541 1101 7 0.608046191769887 1101 8 0.6065048789439942 1101 9 0.740179928061913 1101 10-11 0.6433712736736865 1101 12-13 0.6838934315389551 1101 14-15 1.073471585641677 1101 16-17 1.0464610340563212 1101 18-19 1.0859352036858638 1101 20-21 1.0559638428393114 1101 22-23 0.9961313649766197 1101 24-25 1.0748397019016522 1101 26-27 1.1173819836146635 1101 28-29 0.9444207054636351 1101 30-31 0.8620794759457553 1101 32-33 1.1157465690459318 1101 34-35 1.178742211918582 1101 36-37 1.1075571617781108 1101 38-39 1.0754696384752123 1101 40-41 1.1272179536802653 1101 42-43 1.2227678808831257 1101 44-45 1.2289576027092792 1101 46-47 1.1882737786192266 1101 48-49 1.0678526322269732 1101 50-51 1.1255044538062506 1101 52-53 1.0792910372682627 1101 54-55 1.0408208082989887 1101 56-57 1.1265428485917894 1101 58-59 1.1031750911819103 1101 60-61 1.0781713900669487 1101 62-63 1.1260783502324685 1101 64-65 1.1048273896742202 1101 66-67 1.04254264963118 1101 68-69 1.061446079285588 1101 70-71 1.0226787421267431 1101 72-73 0.8794114795462207 1101 74-75 0.8921885366002797 1101 76-77 0.8996217087620835 1101 78-79 0.960865087766642 1101 80-81 1.0025573790886213 1101 82-83 0.9418053000001692 1101 84-85 0.9755035594665955 1101 86-87 0.9458753195346432 1101 88-89 0.8700093056690505 1101 90-91 0.9926960640652958 1101 92-93 1.0337719853321445 1101 94-95 1.0260367133623767 1101 96-97 0.8774659515574754 1101 98-99 0.932861174733663 1101 100 0.9249856841282522 1102 1 0.6059664464500614 1102 2 4.141516940139631 1102 3 2.316290024496265 1102 4 1.1387224464346133 1102 5 0.5138181089878486 1102 6 0.5882790400910807 1102 7 0.5984791814503723 1102 8 0.5495979830738875 1102 9 0.6790540138423538 1102 10-11 0.6824212501818536 1102 12-13 0.7307186200447475 1102 14-15 0.9959727726495586 1102 16-17 1.0216867021331595 1102 18-19 1.0068745937724088 1102 20-21 1.018987281029979 1102 22-23 0.9475071642203119 1102 24-25 1.0037987953256362 1102 26-27 1.0138962157894298 1102 28-29 0.9715353798609172 1102 30-31 0.947444364153462 1102 32-33 1.05670937894687 1102 34-35 1.1921948850702435 1102 36-37 1.0721940615320662 1102 38-39 1.0206075499174716 1102 40-41 1.0001377720432245 1102 42-43 1.0507021370364455 1102 44-45 1.0441232052076614 1102 46-47 1.0417493774125504 1102 48-49 0.9703500669986553 1102 50-51 0.9795109282140615 1102 52-53 1.0668837505298683 1102 54-55 1.0197625435952347 1102 56-57 0.9528949200943657 1102 58-59 0.903981225976338 1102 60-61 0.9330740926703243 1102 62-63 1.0099698338731145 1102 64-65 0.9334099433403864 1102 66-67 0.9027803690662211 1102 68-69 0.9673527844397825 1102 70-71 1.0102228575972774 1102 72-73 0.9436851126743662 1102 74-75 0.8711859594522124 1102 76-77 0.8630484316369689 1102 78-79 0.8834691694844352 1102 80-81 0.7362958926766296 1102 82-83 0.8206437805108848 1102 84-85 0.836508997353306 1102 86-87 0.8229832642429216 1102 88-89 0.8515306226418744 1102 90-91 0.8480860234686283 1102 92-93 0.9140625514504777 1102 94-95 0.9285684670646965 1102 96-97 0.9158365523026646 1102 98-99 0.9070133456667655 1102 100 0.8530461529913964 1103 1 0.5744663871385036 1103 2 3.994604795765838 1103 3 2.3277596537785 1103 4 1.0704026685905035 1103 5 0.5158978503899618 1103 6 0.5970772910259612 1103 7 0.5639105488241256 1103 8 0.5682822586834462 1103 9 0.6530432246707605 1103 10-11 0.6485342862099159 1103 12-13 0.6521060241051444 1103 14-15 0.9708808091401977 1103 16-17 0.9151367174792142 1103 18-19 0.880618792922192 1103 20-21 0.8732345227620399 1103 22-23 0.8871997240982807 1103 24-25 0.926175630405254 1103 26-27 0.8981467444500666 1103 28-29 0.8851590244745111 1103 30-31 0.8570685001874807 1103 32-33 0.955179257992846 1103 34-35 1.0552748327435069 1103 36-37 0.9142147983215878 1103 38-39 0.9394237284180136 1103 40-41 0.901638582660027 1103 42-43 1.0063439359074664 1103 44-45 0.9784096687955284 1103 46-47 0.9288082886409796 1103 48-49 0.9562013474642157 1103 50-51 0.8984300255902795 1103 52-53 0.9271677948995602 1103 54-55 0.8639145067186149 1103 56-57 0.8654058451866788 1103 58-59 0.9172463524188217 1103 60-61 0.9336815103398735 1103 62-63 0.9742269521971068 1103 64-65 0.9247807934464731 1103 66-67 0.8872519578861215 1103 68-69 0.8733647279751793 1103 70-71 0.9254101068536116 1103 72-73 0.8293597815959188 1103 74-75 0.8169808412916808 1103 76-77 0.7039071309549954 1103 78-79 0.7893654981817804 1103 80-81 0.7734123347152675 1103 82-83 0.8366921907762688 1103 84-85 0.7892011647016943 1103 86-87 0.7587797442950048 1103 88-89 0.7665850869182762 1103 90-91 0.856259814061584 1103 92-93 0.8714370177643502 1103 94-95 0.7228765663060237 1103 96-97 0.7036231588730644 1103 98-99 0.7454369950936233 1103 100 0.8573390708700117 1104 1 0.409953425903808 1104 2 3.6458561906327276 1104 3 2.1663282456323216 1104 4 0.9126513100946241 1104 5 0.41127463082823823 1104 6 0.3973650089231242 1104 7 0.3514968138929433 1104 8 0.3544194098893385 1104 9 0.4045887604002516 1104 10-11 0.46849991947561165 1104 12-13 0.4636028226824962 1104 14-15 0.5850208127618188 1104 16-17 0.5756658924454285 1104 18-19 0.6126488059693287 1104 20-21 0.6498320612959674 1104 22-23 0.5511724210782489 1104 24-25 0.5878756423225724 1104 26-27 0.6252557599719779 1104 28-29 0.5434264643733613 1104 30-31 0.4552617509663648 1104 32-33 0.6565280420403639 1104 34-35 0.6995667511053014 1104 36-37 0.6209463756316538 1104 38-39 0.6618326809484287 1104 40-41 0.6171129766958003 1104 42-43 0.6437394079489991 1104 44-45 0.6965838364024179 1104 46-47 0.690996948934206 1104 48-49 0.597167291818117 1104 50-51 0.5323045904924228 1104 52-53 0.550637697502637 1104 54-55 0.6115694665634734 1104 56-57 0.6088721416429124 1104 58-59 0.5187952544012688 1104 60-61 0.5727006770877949 1104 62-63 0.6586001094638121 1104 64-65 0.5822168849404576 1104 66-67 0.5363395098695776 1104 68-69 0.5578208807891372 1104 70-71 0.6147715480122926 1104 72-73 0.5673411364237424 1104 74-75 0.5539739493723843 1104 76-77 0.5363244565560095 1104 78-79 0.5045031508432842 1104 80-81 0.591055640360203 1104 82-83 0.49840241753016556 1104 84-85 0.5665302635209528 1104 86-87 0.6244650901832998 1104 88-89 0.5413730516740003 1104 90-91 0.5351773199699359 1104 92-93 0.581214396234877 1104 94-95 0.5405691662760042 1104 96-97 0.4896526286766161 1104 98-99 0.46880134438530874 1104 100 0.32638068704288514 1105 1 0.44944551218982554 1105 2 3.7170244650164364 1105 3 2.1684016282897893 1105 4 0.9798382735385687 1105 5 0.43445697403680583 1105 6 0.37534240723112333 1105 7 0.2804648572648816 1105 8 0.26924578685476064 1105 9 0.3482619515885048 1105 10-11 0.33617049877404526 1105 12-13 0.3721898006947626 1105 14-15 0.47394915748965616 1105 16-17 0.5508370700851657 1105 18-19 0.5585915317000882 1105 20-21 0.4882005666914466 1105 22-23 0.5099724606208014 1105 24-25 0.48349890583207866 1105 26-27 0.565183854957354 1105 28-29 0.5286268826919667 1105 30-31 0.4829678145050096 1105 32-33 0.5394396748357124 1105 34-35 0.6623330129810725 1105 36-37 0.5905672873997219 1105 38-39 0.4991829655507658 1105 40-41 0.5803847068907046 1105 42-43 0.4949066937688258 1105 44-45 0.6237477506991311 1105 46-47 0.6591755980356524 1105 48-49 0.6256910017422612 1105 50-51 0.5984054344137846 1105 52-53 0.5560675146703531 1105 54-55 0.4987974814231535 1105 56-57 0.5987632955570987 1105 58-59 0.505584919226088 1105 60-61 0.5944105804166568 1105 62-63 0.6927841280279949 1105 64-65 0.5702050533917387 1105 66-67 0.6220048133586857 1105 68-69 0.6111499997185703 1105 70-71 0.5177871881334184 1105 72-73 0.4717186533594031 1105 74-75 0.4406509699449188 1105 76-77 0.43286500328386524 1105 78-79 0.4460680994899988 1105 80-81 0.5079322467764982 1105 82-83 0.4962694608916962 1105 84-85 0.48908252733222923 1105 86-87 0.5431055026745852 1105 88-89 0.4838456580486721 1105 90-91 0.4548018738154518 1105 92-93 0.4247086053317517 1105 94-95 0.4800240513424434 1105 96-97 0.45261374169547963 1105 98-99 0.4617099594593519 1105 100 0.3694555369372665 1106 1 0.5810843824583358 1106 2 3.8840041604610036 1106 3 2.248685485427707 1106 4 1.1040292933384421 1106 5 0.48946393797244525 1106 6 0.2621178186296831 1106 7 0.2838641815644536 1106 8 0.36591525044852347 1106 9 0.327422212930891 1106 10-11 0.2434676660855004 1106 12-13 0.31261566638755767 1106 14-15 0.3962844522842204 1106 16-17 0.3891841919594512 1106 18-19 0.4161111372817601 1106 20-21 0.31650577852254713 1106 22-23 0.23537258374751957 1106 24-25 0.34248008643460537 1106 26-27 0.2652212161237273 1106 28-29 0.34644675284643256 1106 30-31 0.3715288729200168 1106 32-33 0.3686798962986657 1106 34-35 0.47045510607333085 1106 36-37 0.3944303494362842 1106 38-39 0.3671799658206538 1106 40-41 0.37005013867793224 1106 42-43 0.39200126963960713 1106 44-45 0.3003971765292235 1106 46-47 0.22621854686375542 1106 48-49 0.17761702405265822 1106 50-51 0.11860297642058981 1106 52-53 0.16020456719427045 1106 54-55 0.1173228403148272 1106 56-57 0.20858435414756826 1106 58-59 0.20679243573543715 1106 60-61 0.26905686202593415 1106 62-63 0.2486532886041246 1106 64-65 0.20154406675764136 1106 66-67 0.17642034590237898 1106 68-69 0.09934536765639734 1106 70-71 0.04882555763844465 1106 72-73 0.04400273136167243 1106 74-75 0.05789460603271124 1106 76-77 0.13654750355091494 1106 78-79 0.16018936898527159 1106 80-81 0.10283182832112203 1106 82-83 0.10049631696502814 1106 84-85 0.17575442146596032 1106 86-87 0.31026837878258107 1106 88-89 0.26021296578961284 1106 90-91 0.19768454582001382 1106 92-93 0.23984957923001815 1106 94-95 0.1847931216634784 1106 96-97 0.2330145459926385 1106 98-99 0.2119363583228271 1106 100 0.2862635464695238 1107 1 0.5240796940132597 1107 2 3.6063941873170435 1107 3 2.158749677233722 1107 4 1.0245831822626457 1107 5 0.29288978910729924 1107 6 0.24298922329106176 1107 7 0.204583630746761 1107 8 0.21778241097397455 1107 9 0.24397711354929186 1107 10-11 0.2677048896806582 1107 12-13 0.2791121407136288 1107 14-15 0.2674153985270138 1107 16-17 0.23922048261353268 1107 18-19 0.29477811354353634 1107 20-21 0.24637490378222537 1107 22-23 0.25607584252698246 1107 24-25 0.30235969915131733 1107 26-27 0.27069012461532793 1107 28-29 0.1936337076868213 1107 30-31 0.20822212681326135 1107 32-33 0.30873865036426196 1107 34-35 0.5018209861845833 1107 36-37 0.35929560462837884 1107 38-39 0.23066147455506325 1107 40-41 0.3311798729578399 1107 42-43 0.4175814881806872 1107 44-45 0.45567799646601514 1107 46-47 0.30782982821698823 1107 48-49 0.27313442728095083 1107 50-51 0.25155015525705693 1107 52-53 0.1827089052978721 1107 54-55 0.15719792548631517 1107 56-57 0.18670190980973445 1107 58-59 0.2638060072736863 1107 60-61 0.2616782429926232 1107 62-63 0.3673763952004947 1107 64-65 0.28911837544121255 1107 66-67 0.29390979684302465 1107 68-69 0.22294191939926833 1107 70-71 0.3858895326197356 1107 72-73 0.2898527369090971 1107 74-75 0.237948145593883 1107 76-77 0.16359285313190952 1107 78-79 0.20094267021948298 1107 80-81 0.2872614885520761 1107 82-83 0.28852895978334914 1107 84-85 0.34417176555859186 1107 86-87 0.32446708914799416 1107 88-89 0.28829676183553943 1107 90-91 0.2752039139885092 1107 92-93 0.3400035064982916 1107 94-95 0.2506132758981856 1107 96-97 0.23321415810786306 1107 98-99 0.2163456811625757 1107 100 0.23281039591574526 1108 1 0.47310366518847147 1108 2 3.719119582780241 1108 3 2.1259551128011687 1108 4 1.061010963969089 1108 5 0.3064405559516601 1108 6 0.19506074624551673 1108 7 0.1006389077764851 1108 8 0.10628090720481254 1108 9 0.21497599875740292 1108 10-11 0.15860332656202303 1108 12-13 0.1940614992014318 1108 14-15 0.17935163364479223 1108 16-17 0.15039639819181616 1108 18-19 0.21613305608468636 1108 20-21 0.16298928389628742 1108 22-23 0.04880484557742193 1108 24-25 0.12914179075776389 1108 26-27 0.08045427904608005 1108 28-29 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1.1201487487441781 2212 5 0.536355130413547 2212 6 0.5561419031027626 2212 7 0.42838936727473964 2212 8 0.3361773094193552 2212 9 0.36750365851541744 2212 10-11 0.34504384460286985 2212 12-13 0.3379482553474418 2212 14-15 0.38611711140715244 2212 16-17 0.48896743250820407 2212 18-19 0.3467552199160906 2212 20-21 0.3425165598404689 2212 22-23 0.3547544493425505 2212 24-25 0.44045894719644707 2212 26-27 0.39575794728393987 2212 28-29 0.3533352315100089 2212 30-31 0.3880521186624506 2212 32-33 0.2329552834252837 2212 34-35 0.2533134376122703 2212 36-37 0.30717403496391427 2212 38-39 0.35862103208924623 2212 40-41 0.45574139386254586 2212 42-43 0.3916468252946288 2212 44-45 0.3630976351697157 2212 46-47 0.6195984922494873 2212 48-49 0.591272526730549 2212 50-51 0.23271948860759295 2212 52-53 0.25822803592615173 2212 54-55 0.17006095312248704 2212 56-57 0.4717455702747415 2212 58-59 0.5324901447104224 2212 60-61 0.43690722763991374 2212 62-63 0.303235096697712 2212 64-65 0.2660102449443613 2212 66-67 0.2537766144865117 2212 68-69 0.3470441004472917 2212 70-71 0.37019856172161525 2212 72-73 0.3869269294666253 2212 74-75 0.27860743232111673 2212 76-77 0.3671608797724133 2212 78-79 0.44611899706467995 2212 80-81 0.32321969688879904 2212 82-83 0.40542112758529214 2212 84-85 0.4862213565264355 2212 86-87 0.3265668205178187 2212 88-89 0.3295316846446781 2212 90-91 0.27705926204275855 2212 92-93 0.48514180024522346 2212 94-95 0.46845454787656493 2212 96-97 0.1911609794921887 2212 98-99 0.388892495935945 2212 100 0.37979622752848385 2213 1 -0.6663481229707671 2213 2 -25.787477142700606 2213 3 -14.691567049664652 2213 4 -6.174417807072 2213 5 -2.1929736749593047 2213 6 -1.486316939860977 2213 7 -1.2283986712786827 2213 8 -1.0963775052059717 2213 9 -1.1735785861852222 2213 10-11 -1.2033216346484394 2213 12-13 -1.1628132094398254 2213 14-15 -1.3503079496184256 2213 16-17 -1.5167194358282998 2213 18-19 -1.4788267993259439 2213 20-21 -1.521171216834098 2213 22-23 -1.4295046914867058 2213 24-25 -1.4582375118026007 2213 26-27 -1.59989872260563 2213 28-29 -1.684666468101078 2213 30-31 -1.5189410547108864 2213 32-33 -1.2059007200606544 2213 34-35 -1.2997812528373984 2213 36-37 -1.5725876943036567 2213 38-39 -1.6244064326720036 2213 40-41 -1.5939819857620634 2213 42-43 -1.586988803236295 2213 44-45 -1.3952708899407398 2213 46-47 -1.344863322865777 2213 48-49 -1.5118953835716056 2213 50-51 -1.571790279820398 2213 52-53 -1.7878557588024577 2213 54-55 -1.5211631787534614 2213 56-57 -1.3674753401444875 2213 58-59 -1.4072194580922002 2213 60-61 -1.493449907474833 2213 62-63 -1.6004398786969531 2213 64-65 -1.5072903065620977 2213 66-67 -1.46214065520968 2213 68-69 -1.4378423681337438 2213 70-71 -1.4209771724280031 2213 72-73 -1.4255852648093281 2213 74-75 -1.6035668666977045 2213 76-77 -1.6277421838632762 2213 78-79 -1.4503526840327794 2213 80-81 -1.6437023811458928 2213 82-83 -1.6311045226676981 2213 84-85 -1.4032751987261065 2213 86-87 -1.4452747536168786 2213 88-89 -1.3926504620681825 2213 90-91 -1.3993564121001718 2213 92-93 -1.4643446310484336 2213 94-95 -1.4722872326394842 2213 96-97 -1.4565854099181976 2213 98-99 -1.5599983127233976 2213 100 -1.3971151845795085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 43.0 5 41.0 6 46.0 7 45.0 8 56.0 9 97.0 10 432.0 11 2031.0 12 5920.0 13 11029.0 14 14644.0 15 16414.0 16 17731.0 17 18119.0 18 18599.0 19 19770.0 20 21570.0 21 23708.0 22 26677.0 23 27916.0 24 28731.0 25 29930.0 26 33000.0 27 37166.0 28 43548.0 29 51689.0 30 62522.0 31 75524.0 32 95506.0 33 126152.0 34 172052.0 35 243831.0 36 367326.0 37 629566.0 38 689279.0 39 6061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.92818581310524 21.52949044418594 10.731946171519152 29.810377571189672 2 12.407666016420968 23.358564315457915 37.1221465715388 27.111623096582317 3 14.742082552508137 28.31359585516387 29.478645330853894 27.465676261474105 4 10.135948041386134 19.279140404898943 38.6757794700663 31.909132083648622 5 11.137388346553614 39.224772709163894 35.71529641912183 13.922542525160663 6 28.596015412523773 40.77097609906169 18.202956262572762 12.430052225841774 7 24.795807984393125 33.973696323068914 24.25455423478498 16.975941457752985 8 23.72167509988146 37.51564077797778 21.50925961496246 17.253424507178295 9 24.404063256518448 16.99705092859049 22.716081734347593 35.882804080543465 10-11 22.370652139426518 28.340929378644926 29.720428374080438 19.567990107848125 12-13 23.109970689323116 26.00070211170299 30.158784602453686 20.73054259652021 14-15 20.902708843409545 27.22951182919476 27.202122923193755 24.66565640420194 16-17 20.06431396409057 30.875350023570697 28.11736406721344 20.94297194512529 18-19 19.99446824884581 29.835103745197873 30.23186028024338 19.93856772571294 20-21 21.421731208015775 29.115047904691636 29.241211700551823 20.222009186740763 22-23 20.43363066446323 29.232819156579115 29.539929486878325 20.79362069207933 24-25 20.605526282039413 28.892737538980874 29.385506488981793 21.116229689997922 26-27 20.586288391757297 28.969336313036887 29.397330096463186 21.047045198742627 28-29 20.228984532673657 28.930986587073942 29.892690076176727 20.947338804075674 30-31 20.990853972194977 29.22176459695064 29.13918628815989 20.648195142694494 32-33 19.99514868924612 29.22674735042149 29.865920399076025 20.91218356125637 34-35 20.187151657779825 28.739381733701975 29.90127103767582 21.172195570842383 36-37 20.83506262566834 29.178763141550313 29.22719189839184 20.758982334389508 38-39 20.49696692287318 29.181090604875383 29.53509960681857 20.786842865432863 40-41 20.748948084471422 28.98838161355888 29.253280550123023 21.00938975184668 42-43 20.357869617169513 29.152787611453885 29.51751461050928 20.97182816086732 44-45 20.842834500960475 28.62025130913389 29.36912132664616 21.167792863259475 46-47 20.778399367821837 29.277896927458446 29.190902134630015 20.7528015700897 48-49 20.635223017013548 29.04825600964609 29.325193642865848 20.99132733047451 50-51 20.98711135733084 28.938602641306723 28.900863809454734 21.173422191907704 52-53 20.413498502275523 29.224257769451086 29.050573514702975 21.31167021357042 54-55 20.857158534034287 29.01573372016063 28.803595984952146 21.323511760852938 56-57 20.959256781478107 28.892729767180136 28.828418582657616 21.31959486868414 58-59 20.46923310883908 28.618148201868582 29.43163555053555 21.480983138756788 60-61 21.092930688374135 28.96605439108385 28.675149340003163 21.26586558053885 62-63 20.391975996570938 29.234067723960568 29.084526360908704 21.289429918559794 64-65 20.43294578361798 28.61721735760934 29.23291903634372 21.71691782242896 66-67 20.740251385555926 28.930136844437097 28.895296482563705 21.434315287443273 68-69 20.83605616530035 28.878074342357845 28.94676601295265 21.339103479389156 70-71 20.983078370029588 28.769874577430798 28.6674548623644 21.579592190175216 72-73 20.6091142517886 28.992819879716908 29.09004085166943 21.30802501682506 74-75 21.03347974233479 28.426471314443734 28.79291105173057 21.747137891490905 76-77 20.845043665995938 28.976232311053867 28.850504830212177 21.328219192738015 78-79 20.789567934899548 28.8587515680964 29.01615698640203 21.33552351060202 80-81 20.96816031812079 28.649833106253595 28.740853590062876 21.641152985562734 82-83 20.344381851035305 28.99930739611799 29.03021741590177 21.626093336944933 84-85 20.593134981625795 28.505367394120086 28.82124773161915 22.080249892634974 86-87 20.743545714881336 28.803824568525695 28.724191774283803 21.72843794230917 88-89 20.415492624535325 28.484565046105654 29.102295785243037 21.997646544115984 90-91 20.79162600795322 28.519701675333287 28.759505224316094 21.929167092397396 92-93 20.21752986381326 28.8969872014433 29.004387557398765 21.88109537734468 94-95 20.218206503338926 28.247328131736456 29.20098089214558 22.333484472779045 96-97 20.48628372499018 28.577864297091903 28.903585972625734 22.032266005292183 98-99 20.383253101807583 28.405217146244453 28.958312405481824 22.25321734646614 100 20.4856893687109 28.264530714325183 28.826055634932267 22.42372428203165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 17.5 2 20.0 3 19.0 4 17.5 5 29.0 6 68.0 7 116.5 8 163.5 9 201.0 10 204.0 11 209.0 12 248.0 13 317.5 14 405.5 15 474.0 16 589.5 17 754.5 18 981.5 19 1251.5 20 1642.5 21 2350.0 22 3421.5 23 5093.0 24 7684.5 25 11460.5 26 16894.5 27 24177.5 28 33467.5 29 44301.5 30 55937.0 31 67754.0 32 78807.0 33 89195.0 34 98974.0 35 107545.5 36 115110.0 37 121725.0 38 126591.0 39 129139.5 40 129486.5 41 127790.0 42 124017.0 43 118564.0 44 112184.0 45 105625.0 46 99604.5 47 94119.5 48 89001.5 49 86273.5 50 90846.5 51 85159.0 52 68859.0 53 60205.5 54 54961.0 55 50137.0 56 45457.5 57 40676.0 58 35450.0 59 30672.0 60 26080.0 61 21312.0 62 17142.0 63 13614.5 64 10585.0 65 8163.5 66 6291.5 67 4788.0 68 3477.5 69 2509.0 70 1819.0 71 1351.0 72 1012.5 73 738.0 74 542.0 75 364.0 76 226.5 77 143.0 78 79.5 79 35.5 80 19.5 81 14.0 82 13.0 83 6.0 84 1.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.854845024626421 2 5.358329339211036 3 0.0 4 0.005382636916112461 5 0.1747814203716008 6 0.06013468248956214 7 0.03935838967959939 8 0.04559813438490174 9 0.04429533054533312 10-11 0.04606097259106427 12-13 0.0444153256358197 14-15 0.047038075470740744 16-17 0.03735275745289507 18-19 0.030581605918294997 20-21 0.025216111157966334 22-23 0.025130400379047346 24-25 0.020981998679368317 26-27 0.013319455044010772 28-29 0.020793434965746545 30-31 0.026261782660777992 32-33 0.0016627891110283716 34-35 0.003908411518705863 36-37 0.004474102659571186 38-39 0.006634014288329689 40-41 0.006925430936654249 42-43 0.007576832856438559 44-45 0.008862494540223384 46-47 0.01781069985936575 48-49 0.026964611047913697 50-51 0.023707601448992144 52-53 0.02957021872705094 54-55 0.025918939545102042 56-57 0.01703930284909486 58-59 0.015530793140120666 60-61 0.012702337435794056 62-63 0.018376391000231078 64-65 0.02060487125212477 66-67 0.021530547664449843 68-69 0.029398797169212964 70-71 0.028918816807266632 72-73 0.025661807208345076 74-75 0.02585037092196685 76-77 0.019130645854718173 78-79 0.014793680441417368 80-81 0.014039425586930273 82-83 0.008776783761304394 84-85 0.01143381790779303 86-87 0.00939390136952111 88-89 0.007679685791141345 90-91 0.007953960283682109 92-93 0.0044226761922197926 94-95 0.001834210668866348 96-97 0.0010970979701630493 98-99 6.514019197843105E-4 100 0.10672706190992415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2916786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.97240891617844 #Duplication Level Percentage of deduplicated Percentage of total 1 90.28640676140247 77.62139881663711 2 7.585195993989189 13.042351434091945 3 1.317625679979948 3.3983836127308127 4 0.3751640964457595 1.2901504444121377 5 0.15234232030643538 0.6548618128312148 6 0.07936415419451265 0.4093876510618368 7 0.04776168633304213 0.28743310595653854 8 0.032727510591252035 0.22509303386877444 9 0.024223748280618466 0.18743165933975145 >10 0.09348480540630172 1.326998072967783 >50 0.003091852777625909 0.18319361077711113 >100 0.0018509310006523435 0.3798385618727128 >500 2.8016921339167595E-4 0.1432133626912706 >1k 4.4026590563309094E-4 0.6476602370036614 >5k 4.0024173239371906E-5 0.20260458375717555 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5888 0.20186602651000107 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4543 0.1557536274515854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.856862313519058E-5 0.0 5 0.0 3.428431156759529E-5 0.0 1.0285293470278587E-4 0.0 6 0.0 3.428431156759529E-5 0.0 1.0285293470278587E-4 0.0 7 0.0 6.856862313519058E-5 0.0 1.0285293470278587E-4 0.0 8 0.0 6.856862313519058E-5 0.0 1.0285293470278587E-4 0.0 9 0.0 6.856862313519058E-5 0.0 1.7142155783797647E-4 0.0 10-11 0.0 1.0285293470278587E-4 0.0 2.0570586940557174E-4 0.0 12-13 0.0 1.3713724627038116E-4 0.0 2.0570586940557174E-4 0.0 14-15 0.0 1.7142155783797647E-4 0.0 2.3999018097316705E-4 0.0 16-17 0.0 1.885637136217741E-4 0.0 2.3999018097316705E-4 0.0 18-19 0.0 2.0570586940557174E-4 0.0 2.571323367569647E-4 0.0 20-21 0.0 2.0570586940557174E-4 0.0 3.9426958302734585E-4 0.0 22-23 1.7142155783797645E-5 2.0570586940557174E-4 0.0 4.799803619463341E-4 0.0 24-25 3.428431156759529E-5 2.0570586940557174E-4 0.0 6.342597640005129E-4 0.0 26-27 3.428431156759529E-5 2.0570586940557174E-4 0.0 8.228234776222871E-4 0.0 28-29 3.428431156759529E-5 2.0570586940557174E-4 0.0 0.0014227989300552046 0.0 30-31 3.428431156759529E-5 2.0570586940557174E-4 0.0 0.00407983307654384 0.0 32-33 3.428431156759529E-5 2.0570586940557174E-4 0.0 0.011228112038387458 0.0 34-35 3.428431156759529E-5 2.228480251893694E-4 0.0 0.021633400599152627 0.0 36-37 3.428431156759529E-5 2.3999018097316705E-4 0.0 0.0386384191366799 0.0 38-39 3.428431156759529E-5 2.571323367569647E-4 0.0 0.06443736359129536 0.0 40-41 3.428431156759529E-5 2.742744925407623E-4 0.0 0.09577322436407745 0.0 42-43 3.428431156759529E-5 2.742744925407623E-4 0.0 0.12654339399599424 0.0 44-45 3.428431156759529E-5 2.742744925407623E-4 0.0 0.1652332396000255 0.0 46-47 3.428431156759529E-5 2.742744925407623E-4 0.0 0.21163705530676574 0.0 48-49 3.428431156759529E-5 2.9141664832455996E-4 0.0 0.26181214528594143 0.0 50-51 3.428431156759529E-5 3.4284311567595293E-4 0.0 0.31635848498998553 0.0 52-53 5.1426467351392936E-5 3.4284311567595293E-4 0.0 0.37544749597673605 0.0 54-55 6.856862313519058E-5 3.4284311567595293E-4 0.0 0.44151336436749217 0.0 56-57 6.856862313519058E-5 3.4284311567595293E-4 0.0 0.5189959085102576 0.0 58-59 6.856862313519058E-5 3.4284311567595293E-4 0.0 0.6050838148564892 0.0 60-61 6.856862313519058E-5 3.4284311567595293E-4 0.0 0.6965886424304011 0.0 62-63 6.856862313519058E-5 3.4284311567595293E-4 0.0 0.7898933963616117 0.0 64-65 6.856862313519058E-5 3.4284311567595293E-4 0.0 0.8919406497425592 0.0 66-67 6.856862313519058E-5 3.5998527145975057E-4 0.0 1.0116443235808181 0.0 68-69 6.856862313519058E-5 3.771274272435482E-4 0.0 1.1477701826599551 0.0 70-71 6.856862313519058E-5 3.771274272435482E-4 0.0 1.3033352463979186 0.0 72-73 1.0285293470278587E-4 3.9426958302734585E-4 0.0 1.4628772902777234 0.0 74-75 1.0285293470278587E-4 4.114117388111435E-4 0.0 1.6336645883516994 0.0 76-77 1.0285293470278587E-4 4.114117388111435E-4 0.0 1.8222283019734735 0.0 78-79 1.0285293470278587E-4 4.114117388111435E-4 0.0 2.033865357280239 0.0 80-81 1.0285293470278587E-4 4.114117388111435E-4 0.0 2.26881574445297 0.0 82-83 1.0285293470278587E-4 4.114117388111435E-4 0.0 2.5207882923190112 0.0 84-85 1.0285293470278587E-4 4.114117388111435E-4 0.0 2.7836118247961967 0.0 86-87 1.0285293470278587E-4 4.114117388111435E-4 0.0 3.066885949123453 0.0 88 1.0285293470278587E-4 4.114117388111435E-4 0.0 3.301887762763535 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 180 5.6393837E-6 20.9018 5 GTATCAA 11105 0.0 19.037294 1 TATATCG 185 1.6429907E-4 17.794775 5 CAACGCA 11900 0.0 17.388893 5 TATCAAC 12095 0.0 17.378485 2 AACGCAG 12075 0.0 17.151665 6 TCAACGC 12050 0.0 17.133406 4 ATCAACG 12080 0.0 17.09027 3 GAGTACT 4150 0.0 16.179342 12-13 ACGCAGA 13090 0.0 15.711127 7 TAGACTG 1505 0.0 15.6242695 5 CGCAGAG 13110 0.0 15.615527 8 GTATTAG 1510 0.0 15.153599 1 TGGACCG 280 1.4714968E-5 15.116481 5 GTATATA 2390 0.0 14.98544 1 GTACATG 13450 0.0 14.645622 1 GTACAGG 1475 0.0 14.501447 1 CGTACAC 230 8.6188654E-4 14.312698 3 AGTACTT 4935 0.0 14.224168 12-13 GTATACG 245 9.08975E-4 14.21238 1 >>END_MODULE