FastQCFastQC Report
Wed 25 May 2016
SRR1295075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996863
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2105.2487303E-721.434675
GTACGTC2151.3341419E-418.2850633
CTGTCCG2252.2554947E-517.7993479
GTGTATA15250.017.120031
GTATTAG11450.016.8298911
GTACATG108550.016.320751
GTCCTAC5801.07320375E-1016.0763841
GTATAGG7850.015.8374561
GTATATA24100.015.476021
CATGGGT21100.015.4383794
TGTACCG2659.22924E-515.0986355
TACATGG114400.015.0466682
GTGTAAG12450.014.4795051
TACACTG18000.014.4485555
ACATGGG114850.014.2298033
CTAGTAC6351.2732926E-1014.1508693
CTGTCGC5701.4188117E-1014.0521179
TCTGTCG5501.2605597E-913.6491358
TAGTACT10350.013.5661244
GTATAGA11650.013.3394881
Produced by FastQC (version 0.11.5)