FastQCFastQC Report
Wed 25 May 2016
SRR1295075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996863
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71420.23831586562348697No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50990.17014458118372447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2152.6076574E-517.5015774
GAGTACT39100.016.93248612-13
GTATCAA124850.016.4191171
CGTATAC2155.162678E-415.3136253
TACTGCG1850.00339590315.2287817
AACGCAG133550.015.1200656
CAACGCA133750.015.0513575
GTACTAC6600.015.0045871
TCAACGC136300.014.9078014
TATCAAC136350.014.8767332
GTATCGA2000.004007181614.8545421
AGTACTT46800.014.84889812-13
CTTACAC14700.014.7183433
ATCAACG137800.014.67701153
ACACCGA3304.450818E-614.2303366
CTACACT15350.014.0953254
CGCAGAG145150.013.9426678
ACGCAGA145650.013.8948027
GTACATG151100.013.7633081
CATGGGG41250.013.5690264
Produced by FastQC (version 0.11.5)