##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2996863 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15132657048387 33.0 33.0 33.0 27.0 33.0 2 30.00169210270873 33.0 33.0 33.0 27.0 33.0 3 30.75944379172488 33.0 33.0 33.0 27.0 33.0 4 34.952138953298835 37.0 37.0 37.0 33.0 37.0 5 35.27272384490048 37.0 37.0 37.0 33.0 37.0 6 34.955228183603985 37.0 37.0 37.0 33.0 37.0 7 34.839629639392925 37.0 37.0 37.0 33.0 37.0 8 34.82161613660684 37.0 37.0 37.0 27.0 37.0 9 34.79504201560098 37.0 37.0 37.0 27.0 37.0 10-11 34.76641441400558 37.0 37.0 37.0 27.0 37.0 12-13 34.68114808718316 37.0 37.0 37.0 27.0 37.0 14-15 36.05465114688259 37.0 37.0 40.0 27.0 40.0 16-17 36.038697631489995 37.0 37.0 40.0 27.0 40.0 18-19 36.07297981255733 37.0 37.0 40.0 27.0 40.0 20-21 36.07757361614462 37.0 37.0 40.0 27.0 40.0 22-23 36.038221133231644 37.0 37.0 40.0 27.0 40.0 24-25 36.00524748712237 37.0 37.0 40.0 27.0 40.0 26-27 35.98429858155011 37.0 37.0 40.0 27.0 40.0 28-29 35.92639386585239 37.0 37.0 40.0 27.0 40.0 30-31 35.836232420367566 37.0 37.0 40.0 27.0 40.0 32-33 35.7913267973878 37.0 37.0 40.0 27.0 40.0 34-35 35.692297245486365 37.0 37.0 40.0 27.0 40.0 36-37 35.67988826983415 37.0 37.0 40.0 27.0 40.0 38-39 35.62011643508562 37.0 37.0 40.0 27.0 40.0 40-41 35.56626812770554 37.0 37.0 40.0 27.0 40.0 42-43 35.4773626622238 37.0 37.0 40.0 27.0 40.0 44-45 35.377264326063624 37.0 37.0 40.0 27.0 40.0 46-47 35.2707079035645 37.0 33.0 40.0 27.0 40.0 48-49 35.16385884172884 37.0 33.0 40.0 27.0 40.0 50-51 35.06941208190031 37.0 33.0 40.0 27.0 40.0 52-53 34.978400247191814 37.0 33.0 40.0 24.5 40.0 54-55 34.8630379500164 37.0 33.0 40.0 22.0 40.0 56-57 34.74483801895515 37.0 33.0 40.0 22.0 40.0 58-59 34.62799784307791 37.0 33.0 40.0 22.0 40.0 60-61 34.473883023681765 37.0 33.0 40.0 22.0 40.0 62-63 34.29018093252844 37.0 33.0 40.0 22.0 40.0 64-65 34.12340270476161 37.0 33.0 40.0 22.0 40.0 66-67 33.91181161768156 37.0 33.0 40.0 22.0 40.0 68-69 33.70543515002187 37.0 33.0 37.0 22.0 40.0 70-71 33.477578220959714 37.0 33.0 37.0 22.0 40.0 72-73 33.259720414313236 37.0 33.0 37.0 22.0 40.0 74-75 33.02481544868751 37.0 33.0 37.0 22.0 40.0 76-77 32.786061291423735 37.0 33.0 37.0 22.0 38.5 78-79 32.5542016768868 37.0 33.0 37.0 22.0 37.0 80-81 32.32950855611351 37.0 33.0 37.0 22.0 37.0 82-83 32.12385000582275 35.0 33.0 37.0 22.0 37.0 84-85 31.945601951106873 33.0 33.0 37.0 22.0 37.0 86-87 31.9566755303796 33.0 33.0 37.0 22.0 37.0 88-89 31.957860936586023 33.0 33.0 37.0 22.0 37.0 90-91 31.954499421561813 33.0 33.0 37.0 22.0 37.0 92-93 31.908582908194333 33.0 33.0 37.0 22.0 37.0 94-95 31.92673639068586 33.0 33.0 37.0 22.0 37.0 96-97 31.879964482860913 33.0 33.0 37.0 22.0 37.0 98-99 31.800727961204768 33.0 33.0 37.0 22.0 37.0 100 31.266649159471086 33.0 27.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.688083218540843 1101 2 4.11659545682663 1101 3 2.35319922148037 1101 4 1.1372177296757755 1101 5 0.6226123938003383 1101 6 0.6353332443612629 1101 7 0.6887979993626416 1101 8 0.6390603752001098 1101 9 0.7676824561552777 1101 10-11 0.7038312035905463 1101 12-13 0.7498935785579945 1101 14-15 1.081206877052459 1101 16-17 1.0837870871771216 1101 18-19 1.0447937857366867 1101 20-21 1.0878961163751697 1101 22-23 1.0229854671919227 1101 24-25 1.0217040845887695 1101 26-27 1.0786049514604699 1101 28-29 1.0206518134608444 1101 30-31 0.8944561878877195 1101 32-33 1.1038051516414171 1101 34-35 1.2048152917158887 1101 36-37 0.9849467941078913 1101 38-39 1.0394526071950239 1101 40-41 1.0789963954132418 1101 42-43 1.1495610316315492 1101 44-45 1.143905821342564 1101 46-47 1.180055400446534 1101 48-49 1.1852018086641607 1101 50-51 1.1208283665693344 1101 52-53 1.0971074002226189 1101 54-55 1.1335077936062135 1101 56-57 1.0116194676787345 1101 58-59 1.1294026522743508 1101 60-61 1.0450842558962847 1101 62-63 1.0796992426997534 1101 64-65 1.0804669931484838 1101 66-67 0.9807095085493245 1101 68-69 1.0276748158098954 1101 70-71 1.0619140817790154 1101 72-73 1.064461794207297 1101 74-75 1.0324497295605113 1101 76-77 1.0053236718647511 1101 78-79 0.9871588340668751 1101 80-81 1.0067556664330013 1101 82-83 0.9582351313016524 1101 84-85 0.9763831244005381 1101 86-87 0.9973116506007642 1101 88-89 0.991963218596748 1101 90-91 0.956250655588871 1101 92-93 0.9893528386511612 1101 94-95 0.9812475115895474 1101 96-97 0.9408230485692322 1101 98-99 0.9541335609253387 1101 100 0.967208329035838 1102 1 0.582273502061561 1102 2 3.9873104872825955 1102 3 2.26629680985382 1102 4 1.0605707179032393 1102 5 0.5174237330495117 1102 6 0.5229695693962739 1102 7 0.4791818536907613 1102 8 0.45643959759630803 1102 9 0.5839078561569124 1102 10-11 0.6140178738414264 1102 12-13 0.6115565237325669 1102 14-15 0.9116709568966073 1102 16-17 0.9049245300231163 1102 18-19 0.95088807224964 1102 20-21 0.8589939701799523 1102 22-23 0.8691515546202879 1102 24-25 0.9615396605489153 1102 26-27 0.9412847134918323 1102 28-29 1.0107317051896842 1102 30-31 0.9340866939307233 1102 32-33 0.995893504358385 1102 34-35 1.1512224322606954 1102 36-37 0.9739328040995119 1102 38-39 1.0021506428997213 1102 40-41 0.978942580456021 1102 42-43 1.010304287320345 1102 44-45 1.0496467400509104 1102 46-47 1.0202413025167587 1102 48-49 0.9300398837971144 1102 50-51 0.9087304661283682 1102 52-53 0.9742255620161302 1102 54-55 0.9209063681374658 1102 56-57 0.9348409508052242 1102 58-59 0.9078826681532206 1102 60-61 0.9517672301827247 1102 62-63 0.9770697776966841 1102 64-65 0.9618678678965793 1102 66-67 0.9328981370497331 1102 68-69 0.9317690353773642 1102 70-71 0.9118967554031272 1102 72-73 0.9222242480189138 1102 74-75 0.8498933822868153 1102 76-77 0.8512206610008661 1102 78-79 0.8894979911874614 1102 80-81 0.8627004256873434 1102 82-83 0.8919876472813648 1102 84-85 0.7592399835721473 1102 86-87 0.9345632200822394 1102 88-89 0.8617501091095612 1102 90-91 0.8448573493477731 1102 92-93 0.8899431742050758 1102 94-95 0.8167193512806854 1102 96-97 0.8449481081925576 1102 98-99 0.8900144403633838 1102 100 0.9191268484799764 1103 1 0.5576297413251226 1103 2 3.9952563350576753 1103 3 2.27291891451182 1103 4 1.0544741581651564 1103 5 0.4593775114454033 1103 6 0.5312624868455273 1103 7 0.5211094550808397 1103 8 0.5242914563642032 1103 9 0.5772543429106065 1103 10-11 0.5893838268553324 1103 12-13 0.5848737107602986 1103 14-15 0.9009600554815833 1103 16-17 0.7719525243355889 1103 18-19 0.8574859951586404 1103 20-21 0.778953789144289 1103 22-23 0.67788423049263 1103 24-25 0.7435358710884117 1103 26-27 0.7576785073075243 1103 28-29 0.8025427415757207 1103 30-31 0.7615378383012015 1103 32-33 0.8086223980257046 1103 34-35 0.8955101981286688 1103 36-37 0.8499643471051215 1103 38-39 0.7595187731302318 1103 40-41 0.665415321524577 1103 42-43 0.7656031261954297 1103 44-45 0.8073943032122415 1103 46-47 0.7979609330372028 1103 48-49 0.7610421557168294 1103 50-51 0.7666941471079909 1103 52-53 0.8164080741724007 1103 54-55 0.755216183980032 1103 56-57 0.7179099698892344 1103 58-59 0.751683748875962 1103 60-61 0.8136123539564295 1103 62-63 0.8056519415135668 1103 64-65 0.7475482628848837 1103 66-67 0.7507409264684171 1103 68-69 0.7328851763177155 1103 70-71 0.7462924675293792 1103 72-73 0.6901908913343888 1103 74-75 0.828780319197179 1103 76-77 0.6515267181232574 1103 78-79 0.6519304460158963 1103 80-81 0.6255714074167145 1103 82-83 0.6966073494210256 1103 84-85 0.6981705182761608 1103 86-87 0.7497833207768281 1103 88-89 0.6950320812863247 1103 90-91 0.7614817003403651 1103 92-93 0.7307169777622029 1103 94-95 0.7321350921577299 1103 96-97 0.7266818851793637 1103 98-99 0.7376713549020444 1103 100 0.5866910943177643 1104 1 0.38817816684318274 1104 2 3.4802374574767896 1104 3 2.059796732439512 1104 4 0.90762995441073 1104 5 0.2763411551904653 1104 6 0.3482957834962903 1104 7 0.308663500732834 1104 8 0.25340528820836283 1104 9 0.36193212475026115 1104 10-11 0.38166419193323264 1104 12-13 0.39247139509361517 1104 14-15 0.4853174822915278 1104 16-17 0.5120048471536549 1104 18-19 0.5041469552173652 1104 20-21 0.5168664360866586 1104 22-23 0.44532579419407625 1104 24-25 0.45216960976684817 1104 26-27 0.4764276387609314 1104 28-29 0.4087348196380205 1104 30-31 0.4174620789479846 1104 32-33 0.533483233598588 1104 34-35 0.5778464605092779 1104 36-37 0.42809675694049787 1104 38-39 0.42157928492586194 1104 40-41 0.45466728363629727 1104 42-43 0.4485686815526435 1104 44-45 0.5914343936073081 1104 46-47 0.5258816990625519 1104 48-49 0.40089130569761267 1104 50-51 0.39332768538327656 1104 52-53 0.4598654597658154 1104 54-55 0.47548763744958933 1104 56-57 0.47656866953957433 1104 58-59 0.5570147773313252 1104 60-61 0.3963309436015834 1104 62-63 0.5073762907918109 1104 64-65 0.516041955236652 1104 66-67 0.4687306551541539 1104 68-69 0.4240322895866413 1104 70-71 0.39656284744513215 1104 72-73 0.3875305636138435 1104 74-75 0.4979556747133884 1104 76-77 0.5170158760756962 1104 78-79 0.44660733508027306 1104 80-81 0.4342986433994511 1104 82-83 0.42629036779609564 1104 84-85 0.4592763985716708 1104 86-87 0.4095091309776393 1104 88-89 0.393300116591508 1104 90-91 0.4609915367469135 1104 92-93 0.5492404526523735 1104 94-95 0.4005023579602245 1104 96-97 0.4262639121581877 1104 98-99 0.43776541049298956 1104 100 0.48886402329302214 1105 1 0.45323971658761764 1105 2 3.6274339384127927 1105 3 2.1329731374814074 1105 4 1.0294077196796536 1105 5 0.41991603174025016 1105 6 0.38271151906510426 1105 7 0.4052342417914758 1105 8 0.43678761282041734 1105 9 0.4350218704910489 1105 10-11 0.3608053416792174 1105 12-13 0.40422577178124186 1105 14-15 0.5045115019486346 1105 16-17 0.4296238654464304 1105 18-19 0.5054267515757402 1105 20-21 0.4838409850671894 1105 22-23 0.4768867417119864 1105 24-25 0.43116552539115816 1105 26-27 0.4922038992433144 1105 28-29 0.5674499030303082 1105 30-31 0.456172854038293 1105 32-33 0.5431299322842236 1105 34-35 0.6574269492159956 1105 36-37 0.5002861671884133 1105 38-39 0.5820085613489923 1105 40-41 0.48116295389920083 1105 42-43 0.6369029662281136 1105 44-45 0.549296380981211 1105 46-47 0.5598965265560167 1105 48-49 0.48921200391624353 1105 50-51 0.4624418510574415 1105 52-53 0.47245424656001234 1105 54-55 0.4248941530152308 1105 56-57 0.3948044220577316 1105 58-59 0.29792655842877025 1105 60-61 0.4506649350765244 1105 62-63 0.465775897091639 1105 64-65 0.4047185562412423 1105 66-67 0.3740951439570068 1105 68-69 0.41466457388543887 1105 70-71 0.4589714111830361 1105 72-73 0.3817275700427558 1105 74-75 0.47424451480645047 1105 76-77 0.3644609052689276 1105 78-79 0.4251403705899648 1105 80-81 0.39145501273355876 1105 82-83 0.4941184383540751 1105 84-85 0.40428548655483 1105 86-87 0.42522216208714525 1105 88-89 0.346815973176259 1105 90-91 0.2896787805969261 1105 92-93 0.3755860335515102 1105 94-95 0.423301443230681 1105 96-97 0.340785561641578 1105 98-99 0.3494041995346784 1105 100 0.33321134516397066 1106 1 0.5635949864921024 1106 2 3.807503766584933 1106 3 2.2290906863408573 1106 4 0.927415971232783 1106 5 0.38614860152669195 1106 6 0.40438584504914843 1106 7 0.32749438035079237 1106 8 0.2770819977100558 1106 9 0.303711638326682 1106 10-11 0.31619157875635295 1106 12-13 0.3361584953899879 1106 14-15 0.3579868486450337 1106 16-17 0.2800889453982549 1106 18-19 0.33100765137660915 1106 20-21 0.2966483613833262 1106 22-23 0.33935606668246976 1106 24-25 0.33646667130771135 1106 26-27 0.3457825095959137 1106 28-29 0.24258681680635164 1106 30-31 0.22527310086943686 1106 32-33 0.36040841027809734 1106 34-35 0.4581897766846055 1106 36-37 0.28994389467252546 1106 38-39 0.2824646862193063 1106 40-41 0.3428334283635053 1106 42-43 0.3510265964345223 1106 44-45 0.3477802534875636 1106 46-47 0.3153537723020605 1106 48-49 0.2666580647535639 1106 50-51 0.21744552371403358 1106 52-53 0.20166051153980646 1106 54-55 0.2520234352970476 1106 56-57 0.29292427228966034 1106 58-59 0.255562273821738 1106 60-61 0.1301608033378514 1106 62-63 0.229542027566346 1106 64-65 0.1667453976373352 1106 66-67 0.056234740680238815 1106 68-69 0.06786932449059435 1106 70-71 0.051773195044809484 1106 72-73 0.13115277543726478 1106 74-75 0.08601528379873002 1106 76-77 0.24594372844482137 1106 78-79 0.14813802152065136 1106 80-81 0.1443459539114329 1106 82-83 0.12441629318875513 1106 84-85 0.1353862938238244 1106 86-87 0.15520514623987225 1106 88-89 0.22230065652887632 1106 90-91 0.28248832711922844 1106 92-93 0.36154708908255273 1106 94-95 0.23796329649135828 1106 96-97 0.3338967787456646 1106 98-99 0.30911880549916404 1106 100 0.36074758879942337 1107 1 0.45261965768202117 1107 2 3.5462062217797126 1107 3 2.0298883444260696 1107 4 0.9605811811584957 1107 5 0.3879829981020535 1107 6 0.26133498829467783 1107 7 0.2314627334981907 1107 8 0.07901362429150538 1107 9 0.17878252775349068 1107 10-11 0.19757483478105797 1107 12-13 0.1951109058689795 1107 14-15 0.24044391552792632 1107 16-17 0.20089963664469934 1107 18-19 0.31421608656256694 1107 20-21 0.24403320332792333 1107 22-23 0.2103389196688923 1107 24-25 0.2360794922180034 1107 26-27 0.1646577823622053 1107 28-29 0.0862066849245906 1107 30-31 0.12041095665804846 1107 32-33 0.2180640510255074 1107 34-35 0.40591555563359805 1107 36-37 0.2640437241563234 1107 38-39 0.170211940670022 1107 40-41 0.22965156031681033 1107 42-43 0.22138599211557164 1107 44-45 0.2413596269895848 1107 46-47 0.18809370528799718 1107 48-49 0.10097762164993185 1107 50-51 0.14435163248667493 1107 52-53 0.13041425276060892 1107 54-55 0.14323239514823172 1107 56-57 0.24002221774831867 1107 58-59 0.1846883007942921 1107 60-61 0.11149271371549219 1107 62-63 0.19612778052056967 1107 64-65 0.13367530713258446 1107 66-67 0.16536781486074403 1107 68-69 0.09750444367306699 1107 70-71 0.12243444017079241 1107 72-73 0.19764758244393477 1107 74-75 0.19560554410423237 1107 76-77 0.10690393735697512 1107 78-79 0.18494505203470624 1107 80-81 0.2389205219569348 1107 82-83 0.22772563103738364 1107 84-85 0.33011093997290075 1107 86-87 0.24052211558272774 1107 88-89 0.2682235068919674 1107 90-91 0.27262716069303394 1107 92-93 0.3496210668796742 1107 94-95 0.2131789815968297 1107 96-97 0.1883285782281554 1107 98-99 0.2849180733526886 1107 100 0.18672242871690514 1108 1 0.5743144691876552 1108 2 3.671405450420348 1108 3 2.1796716626586026 1108 4 1.0868274666249462 1108 5 0.37699219481022794 1108 6 0.28335376131757073 1108 7 0.21314888139507815 1108 8 0.17682886780575302 1108 9 0.15219984439887924 1108 10-11 0.2999165011724827 1108 12-13 0.26871532926021047 1108 14-15 0.21649031189446788 1108 16-17 0.21412369589089764 1108 18-19 0.2650751817906638 1108 20-21 0.2258129925797121 1108 22-23 0.20954869887004435 1108 24-25 0.2370456888540602 1108 26-27 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0.21409915900807874 2210 78-79 0.46240626203400126 2210 80-81 0.3090252694465647 2210 82-83 0.3690214583634308 2210 84-85 0.3808075882863129 2210 86-87 0.4177399636366914 2210 88-89 0.49949213240624246 2210 90-91 0.38174151574316895 2210 92-93 0.5239813757022169 2210 94-95 0.43762050951520237 2210 96-97 0.3920045185856402 2210 98-99 0.40859072184235146 2210 100 0.2811379872783881 2211 1 0.3832036545950075 2211 2 3.897685999508518 2211 3 2.1274252446984825 2211 4 1.0134428372827102 2211 5 0.5283315948101759 2211 6 0.38600819963031796 2211 7 0.21313447666305052 2211 8 0.3122035285679985 2211 9 0.15957147865110244 2211 10-11 0.26390517652596657 2211 12-13 0.26484712800942845 2211 14-15 0.15669791188162918 2211 16-17 0.10986704703302763 2211 18-19 0.24460706843402136 2211 20-21 0.2881950601540737 2211 22-23 0.14752930201385794 2211 24-25 0.25384216237348767 2211 26-27 0.2634746905615657 2211 28-29 0.11337645056633505 2211 30-31 0.1718479329566307 2211 32-33 0.2626483038277101 2211 34-35 0.45374661358739843 2211 36-37 0.257367288412226 2211 38-39 0.23774741337683025 2211 40-41 0.19567248551722827 2211 42-43 0.3623369500018754 2211 44-45 0.3399839032151064 2211 46-47 0.3950867592492102 2211 48-49 0.21734074122041847 2211 50-51 -0.004992823770443522 2211 52-53 0.254907881961806 2211 54-55 0.13649295753756974 2211 56-57 0.047350200562377154 2211 58-59 0.21675502505732425 2211 60-61 0.3778057152628307 2211 62-63 0.3213576402896976 2211 64-65 0.1502520507864844 2211 66-67 0.16021889984890691 2211 68-69 0.14109578501523856 2211 70-71 0.13681286048645092 2211 72-73 0.16938341794138267 2211 74-75 0.16137658147963663 2211 76-77 0.05255150946116771 2211 78-79 0.1315160471194332 2211 80-81 0.22343592983336436 2211 82-83 -0.25294183632588485 2211 84-85 0.1072150861188419 2211 86-87 0.13079873387135166 2211 88-89 0.1840287011366577 2211 90-91 0.23407126971205727 2211 92-93 0.36513801545436664 2211 94-95 0.3060080309990276 2211 96-97 0.24564408580434005 2211 98-99 0.4003465332903211 2211 100 0.3589602361191524 2212 1 0.6309796018767209 2212 2 4.133061831788428 2212 3 2.3479558046020976 2212 4 1.2223390481558596 2212 5 0.5968918361314195 2212 6 0.500355587218337 2212 7 0.4971679367113353 2212 8 0.38552265400667096 2212 9 0.23833128460837827 2212 10-11 0.36232070724559406 2212 12-13 0.4545019856946979 2212 14-15 0.592051839056289 2212 16-17 0.606067423983383 2212 18-19 0.6211416807821095 2212 20-21 0.49515358192455494 2212 22-23 0.5095212062520389 2212 24-25 0.4642165114952306 2212 26-27 0.5527731339300246 2212 28-29 0.4839724976075459 2212 30-31 0.6008124147411635 2212 32-33 0.5522739547059672 2212 34-35 0.48580473513335676 2212 36-37 0.6234563600464824 2212 38-39 0.5195684577940014 2212 40-41 0.5670224332644267 2212 42-43 0.4493965090703682 2212 44-45 0.5052524461148806 2212 46-47 0.5000925321571543 2212 48-49 0.6040224372232004 2212 50-51 0.6312313749818799 2212 52-53 0.5252328533254413 2212 54-55 0.4633226042721077 2212 56-57 0.6463069699244315 2212 58-59 0.582188312602895 2212 60-61 0.5648972192764887 2212 62-63 0.8071024366863142 2212 64-65 0.8386765717264026 2212 66-67 0.5876212738146904 2212 68-69 0.8007885659139831 2212 70-71 0.622411166491645 2212 72-73 0.7512026369331153 2212 74-75 0.6116807429932791 2212 76-77 0.6924525714024199 2212 78-79 0.6772511687612237 2212 80-81 0.6617073432146441 2212 82-83 0.6667859927412785 2212 84-85 0.7239273123620684 2212 86-87 0.5078595251020062 2212 88-89 0.7780449565501861 2212 90-91 0.7271597526466103 2212 92-93 0.8687126548187543 2212 94-95 0.7349040593296259 2212 96-97 0.4995379464422349 2212 98-99 0.627719700449088 2212 100 0.5970586904401642 2213 1 -0.6002596408083072 2213 2 -25.730650079568914 2213 3 -14.68054601430789 2213 4 -6.240827905372541 2213 5 -2.3942660681409933 2213 6 -1.428500954897892 2213 7 -1.4779487548272812 2213 8 -1.2107239072283775 2213 9 -1.274744604225944 2213 10-11 -1.192458758857839 2213 12-13 -1.216789869498136 2213 14-15 -1.2612065844279172 2213 16-17 -1.4628864286253034 2213 18-19 -1.4303316839868216 2213 20-21 -1.473017996355324 2213 22-23 -1.4996436685220829 2213 24-25 -1.482230535100335 2213 26-27 -1.4491139553848953 2213 28-29 -1.463810243964204 2213 30-31 -1.5070781502890398 2213 32-33 -1.328860754292272 2213 34-35 -1.308937589924696 2213 36-37 -1.5110320089419318 2213 38-39 -1.4221211737038928 2213 40-41 -1.5268045962624655 2213 42-43 -1.4357369412326335 2213 44-45 -1.3129364153857281 2213 46-47 -1.3822453273934272 2213 48-49 -1.514869672569354 2213 50-51 -1.4013136448282637 2213 52-53 -1.5444734749556304 2213 54-55 -1.5369025543759278 2213 56-57 -1.457873423989966 2213 58-59 -1.4180573475207794 2213 60-61 -1.540201569229339 2213 62-63 -1.6022220396916964 2213 64-65 -1.4990850008798091 2213 66-67 -1.5616837023225472 2213 68-69 -1.5144999327581843 2213 70-71 -1.5752215090462798 2213 72-73 -1.6237990317916484 2213 74-75 -1.6315263552850752 2213 76-77 -1.345065009804884 2213 78-79 -1.424599566192839 2213 80-81 -1.3957252760634127 2213 82-83 -1.4254002035675022 2213 84-85 -1.4614913706803456 2213 86-87 -1.3313072329472035 2213 88-89 -1.3454051557311537 2213 90-91 -1.2462813008431688 2213 92-93 -1.2884809792346736 2213 94-95 -1.5077730996426304 2213 96-97 -1.4115806474426051 2213 98-99 -1.3003610038481135 2213 100 -1.462386530093731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 20.0 4 31.0 5 50.0 6 32.0 7 56.0 8 63.0 9 95.0 10 500.0 11 2422.0 12 6968.0 13 13126.0 14 17214.0 15 19048.0 16 20196.0 17 20721.0 18 20872.0 19 22444.0 20 23911.0 21 26493.0 22 28844.0 23 30516.0 24 31191.0 25 32893.0 26 35958.0 27 40229.0 28 46731.0 29 55042.0 30 66468.0 31 80994.0 32 101396.0 33 134129.0 34 180628.0 35 252562.0 36 377042.0 37 633527.0 38 668370.0 39 6079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.751485932921845 21.407685944551837 10.446184909449098 30.394643213077217 2 12.333789665824206 23.415122156697556 36.683270148834595 27.567818028643636 3 14.667203672640358 28.226815840430476 29.261431036387048 27.844549450542118 4 10.062652564417014 19.209393045982477 38.487662200064406 32.2402921895361 5 11.231121290642136 38.95923244880368 35.571433871167734 14.238212389386446 6 28.823467745004884 40.56214789802171 18.07790130026916 12.536483056704235 7 24.89946742016727 33.84507284376935 24.080753189053322 17.17470654701006 8 23.767552503952487 37.38145884074691 21.49110156817502 17.359887087125582 9 24.401606886886995 16.88566520430257 22.619997422892062 36.09273048591837 10-11 22.399888236154204 28.278739966123673 29.567896536322248 19.753475261399878 12-13 23.18054579828854 25.99294205343416 29.959803119910816 20.866709028366483 14-15 21.02178411375924 27.105682770919376 27.09922319891025 24.773309916411137 16-17 20.140321901205212 30.875441364084562 27.959284017162545 21.02495271754768 18-19 20.057190244680722 29.77861393931272 30.09625778400986 20.067938031996697 20-21 21.513598559749035 29.021330580017086 29.044260048205285 20.42081081202859 22-23 20.426337630686618 29.23566988463348 29.398262067625865 20.93973041705404 24-25 20.75575444113327 28.770486851735722 29.23419967152285 21.239559035608167 26-27 20.73036284867909 28.863417431747596 29.197560662639816 21.208659056933502 28-29 20.226128892300505 28.84395754764209 29.771818576244037 21.15809498381337 30-31 21.150552204981693 29.21386303180394 28.88425890386727 20.7513258593471 32-33 20.05853869817383 29.228906230969415 29.716689785609486 20.995865285247266 34-35 20.278536836530836 28.58543890799652 29.81569805059919 21.320326204873453 36-37 20.92225141132413 29.18328818708982 29.02743562384823 20.86702477773783 38-39 20.620736431036654 29.056097868225944 29.390000442155618 20.93316525858178 40-41 20.88477513881237 28.883284150979538 29.100191512515057 21.13174919769304 42-43 20.46020938145307 29.050141558734527 29.400651263616933 21.088997796195468 44-45 21.06104895866826 28.409379302243742 29.18999225627411 21.339579482813882 46-47 20.93600547332588 29.226942780382796 29.018088674554043 20.818963071737283 48-49 20.713894251437207 28.950584896037768 29.22876430865849 21.10675654386654 50-51 21.08296111321933 28.83605326861159 28.71641883699112 21.36456678117796 52-53 20.483797671198424 29.164842709682176 28.89550065762585 21.455858961493547 54-55 20.96525027581464 28.902470508151445 28.5810052285878 21.551273987446116 56-57 21.043371291320234 28.813172414874867 28.617836905362232 21.525619388442667 58-59 20.52724540681429 28.47320429612313 29.345394382801494 21.65415591426109 60-61 21.22585540113669 28.932804516997894 28.378892265602225 21.46244781626319 62-63 20.424006852406762 29.205396820999894 28.943458800037845 21.427137526555494 64-65 20.52710458103456 28.35015238186463 29.17863646808491 21.944106569015894 66-67 20.81957919986196 28.849061676201735 28.718013243684837 21.61334588025147 68-69 21.033183790046163 28.693118950497848 28.778782854936114 21.49491440451988 70-71 21.149433228961083 28.669342227838584 28.41672265785761 21.764501885342725 72-73 20.66430215347819 28.93914151583063 28.943130025584875 21.45342630510631 74-75 21.222222759960022 28.24320560410308 28.625036526767133 21.909535109169767 76-77 20.97011987660071 28.911408593862507 28.65419045789801 21.464281071638773 78-79 20.916092007343448 28.769632223595345 28.879880710703098 21.43439505835811 80-81 21.147917331343418 28.58925446295676 28.496594630168293 21.76623357553153 82-83 20.487438365656864 28.963314978670024 28.825475634171944 21.72377102150117 84-85 20.797869260610273 28.339654836103968 28.613823008267765 22.248652895018 86-87 20.99466024779574 28.76710157342652 28.44123018867169 21.797007990106056 88-89 20.57887873009686 28.39982673916569 28.905510108328063 22.11578442240938 90-91 21.013504325271786 28.42525735137692 28.49581989116116 22.065418432190135 92-93 20.358093416428297 28.89200635892699 28.778849760338932 21.971050464305783 94-95 20.39658804146072 28.213560241089493 28.950443179211245 22.439408538238546 96-97 20.721535996882036 28.562690764137695 28.606653899838797 22.109119339141472 98-99 20.60559425648637 28.333196800698335 28.767086948041797 22.294121994773498 100 20.763369029060414 28.166677745380426 28.57703552993608 22.492917695623085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21.0 1 19.0 2 17.0 3 24.0 4 34.5 5 39.0 6 61.0 7 102.5 8 153.0 9 186.0 10 175.5 11 178.5 12 218.5 13 266.5 14 319.0 15 412.5 16 528.0 17 669.0 18 836.0 19 1113.5 20 1577.0 21 2196.5 22 3090.5 23 4600.5 24 6925.0 25 10373.0 26 15390.0 27 22440.5 28 31841.0 29 42774.5 30 54840.5 31 67303.5 32 79515.0 33 90863.0 34 100836.0 35 110024.5 36 117581.0 37 123364.0 38 128041.0 39 131063.5 40 132377.0 41 131236.5 42 127677.0 43 122388.5 44 115532.0 45 107987.0 46 101716.0 47 96268.5 48 90584.0 49 87969.5 50 94474.0 51 89382.5 52 72192.0 53 64058.0 54 59156.5 55 53884.0 56 48614.5 57 43459.5 58 38433.0 59 34524.5 60 29919.0 61 24663.5 62 20403.0 63 16037.0 64 11945.5 65 9081.0 66 6690.0 67 4716.0 68 3377.0 69 2416.5 70 1729.0 71 1255.5 72 912.0 73 653.5 74 458.0 75 294.5 76 176.5 77 103.0 78 53.0 79 26.5 80 13.0 81 5.5 82 5.0 83 3.5 84 2.0 85 2.0 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.727293840258964 2 4.940599553599881 3 0.0 4 0.005939544116631291 5 0.16023421824754752 6 0.05462378493778328 7 0.035637264699787743 8 0.042677960253772025 9 0.0419438592955367 10-11 0.04207733219703403 12-13 0.04112633777386554 14-15 0.04366232290231486 16-17 0.03460284971318342 18-19 0.029947982273463954 20-21 0.02397506993145833 22-23 0.02390833348070966 24-25 0.020421353929091852 26-27 0.013130396684800073 28-29 0.01878631088574953 30-31 0.023124180184412833 32-33 0.00140146546572199 34-35 0.0032033496359359774 36-37 0.004020871157607138 38-39 0.006022964680067124 40-41 0.006406699271871955 42-43 0.007574587159973612 44-45 0.008125162878650108 46-47 0.01711789961703288 48-49 0.02586037466510815 50-51 0.021756082944065176 52-53 0.028646621483864963 54-55 0.024642434438944993 56-57 0.016016748179679883 58-59 0.014698703277393727 60-61 0.011995877022072747 62-63 0.018135630490950037 64-65 0.020271196914907356 66-67 0.02143908480300901 68-69 0.028429728018931795 70-71 0.028563200920429126 72-73 0.025243062495682988 74-75 0.025776954101672314 76-77 0.019136677252180028 78-79 0.015015701418449892 80-81 0.013981286431845565 82-83 0.008625686259265105 84-85 0.011495353641457751 86-87 0.009409839555561933 88-89 0.007507850709224946 90-91 0.007691375948783778 92-93 0.004371237524037635 94-95 0.0016850953814038212 96-97 0.0011345196627273253 98-99 7.174168455481616E-4 100 0.10457601832315991 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2996863.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.3662656063047 #Duplication Level Percentage of deduplicated Percentage of total 1 91.34155033982947 78.88828597541362 2 6.817691866408468 11.77637173112354 3 1.113349012495533 2.8846738957711873 4 0.29758265189512334 1.0280440941361093 5 0.12662989252168041 0.5468275465612633 6 0.07257594128098506 0.3760867812780677 7 0.041880286719773833 0.2531930776555727 8 0.03345894102358949 0.2311779029879211 9 0.025654634640487717 0.1994125490453761 >10 0.12007712844173976 1.805821295090116 >50 0.005641075874436458 0.34654524008257054 >100 0.0029774158777893123 0.47829743557463905 >500 4.654064963228935E-4 0.24659890817651706 >1k 3.8783874545599977E-4 0.5286367775051638 >5k 7.756774909119996E-5 0.4100267895983215 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7142 0.23831586562348697 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5099 0.17014458118372447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.33682253743331E-5 0.0 5 0.0 0.0 0.0 3.33682253743331E-5 0.0 6 0.0 0.0 0.0 3.33682253743331E-5 0.0 7 3.33682253743331E-5 0.0 0.0 6.67364507486662E-5 0.0 8 6.67364507486662E-5 0.0 0.0 6.67364507486662E-5 0.0 9 1.334729014973324E-4 0.0 0.0 1.0010467612299928E-4 0.0 10-11 1.334729014973324E-4 0.0 0.0 1.0010467612299928E-4 0.0 12-13 1.334729014973324E-4 0.0 0.0 1.334729014973324E-4 0.0 14-15 1.334729014973324E-4 0.0 0.0 2.0020935224599856E-4 0.0 16-17 1.334729014973324E-4 0.0 0.0 2.0020935224599856E-4 0.0 18-19 1.334729014973324E-4 0.0 1.668411268716655E-5 2.0020935224599856E-4 0.0 20-21 1.334729014973324E-4 3.33682253743331E-5 3.33682253743331E-5 2.3357757762033167E-4 0.0 22-23 1.334729014973324E-4 3.33682253743331E-5 3.33682253743331E-5 3.503663664304975E-4 0.0 24-25 1.334729014973324E-4 3.33682253743331E-5 6.67364507486662E-5 4.6715515524066334E-4 0.0 26-27 2.3357757762033167E-4 3.33682253743331E-5 1.0010467612299928E-4 6.673645074866619E-4 0.0 28-29 2.669458029946648E-4 3.33682253743331E-5 1.0010467612299928E-4 0.0013347290149733238 0.0 30-31 3.0031402836899784E-4 3.33682253743331E-5 1.0010467612299928E-4 0.004237764622540303 0.0 32-33 3.0031402836899784E-4 3.33682253743331E-5 1.0010467612299928E-4 0.011411933078021918 0.0 34-35 3.3368225374333095E-4 3.33682253743331E-5 1.0010467612299928E-4 0.02265702502917217 0.0 36-37 3.503663664304975E-4 3.33682253743331E-5 1.0010467612299928E-4 0.04050902560444038 0.0 38-39 4.004187044919971E-4 3.33682253743331E-5 1.0010467612299928E-4 0.06660297784716886 0.0 40-41 4.004187044919971E-4 3.33682253743331E-5 1.1678878881016583E-4 0.10023814902449663 0.0 42-43 4.004187044919971E-4 3.33682253743331E-5 1.334729014973324E-4 0.1320547519189232 0.0 44-45 4.004187044919971E-4 3.33682253743331E-5 1.334729014973324E-4 0.17207993825543577 3.33682253743331E-5 46-47 4.1710281717916367E-4 3.33682253743331E-5 1.334729014973324E-4 0.21886219023025077 5.0052338061499646E-5 48-49 4.337869298663302E-4 3.33682253743331E-5 1.6684112687166547E-4 0.26893121240443757 6.67364507486662E-5 50-51 4.337869298663302E-4 3.33682253743331E-5 2.0020935224599856E-4 0.32283758049667266 6.67364507486662E-5 52-53 4.337869298663302E-4 3.33682253743331E-5 2.0020935224599856E-4 0.38221633755029844 6.67364507486662E-5 54-55 4.337869298663302E-4 3.33682253743331E-5 2.0020935224599856E-4 0.445782806888403 6.67364507486662E-5 56-57 4.504710425534968E-4 3.33682253743331E-5 2.3357757762033167E-4 0.5203107382619759 6.67364507486662E-5 58-59 4.6715515524066334E-4 3.33682253743331E-5 2.669458029946648E-4 0.6060337092486376 6.67364507486662E-5 60-61 5.005233806149964E-4 3.33682253743331E-5 2.669458029946648E-4 0.696428231787706 6.67364507486662E-5 62-63 5.505757186764961E-4 3.33682253743331E-5 3.0031402836899784E-4 0.7904098385545152 6.67364507486662E-5 64-65 5.672598313636626E-4 3.33682253743331E-5 3.0031402836899784E-4 0.8962204812165254 6.67364507486662E-5 66-67 5.672598313636626E-4 3.33682253743331E-5 3.0031402836899784E-4 1.0202668590456088 6.67364507486662E-5 68-69 5.839439440508291E-4 3.33682253743331E-5 3.0031402836899784E-4 1.1569764784042516 6.67364507486662E-5 70-71 6.173121694251622E-4 3.33682253743331E-5 3.0031402836899784E-4 1.31602612465101 6.67364507486662E-5 72-73 6.339962821123288E-4 3.33682253743331E-5 3.0031402836899784E-4 1.4760601335463117 6.67364507486662E-5 74-75 6.673645074866619E-4 3.33682253743331E-5 3.0031402836899784E-4 1.6426176305022953 6.67364507486662E-5 76-77 7.00732732860995E-4 3.33682253743331E-5 3.0031402836899784E-4 1.8312982608814616 6.67364507486662E-5 78-79 7.00732732860995E-4 3.33682253743331E-5 3.0031402836899784E-4 2.03963277600611 6.67364507486662E-5 80-81 7.00732732860995E-4 3.33682253743331E-5 3.0031402836899784E-4 2.2729434078234476 6.67364507486662E-5 82-83 7.00732732860995E-4 3.33682253743331E-5 3.0031402836899784E-4 2.5289110646699564 6.67364507486662E-5 84-85 7.00732732860995E-4 3.33682253743331E-5 3.0031402836899784E-4 2.793003884395116 6.67364507486662E-5 86-87 7.34100958235328E-4 3.33682253743331E-5 3.0031402836899784E-4 3.0779852132046077 6.67364507486662E-5 88 7.674691836096611E-4 3.33682253743331E-5 3.0031402836899784E-4 3.3177359125191908 6.67364507486662E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 215 2.6076574E-5 17.501577 4 GAGTACT 3910 0.0 16.932486 12-13 GTATCAA 12485 0.0 16.419117 1 CGTATAC 215 5.162678E-4 15.313625 3 TACTGCG 185 0.003395903 15.228781 7 AACGCAG 13355 0.0 15.120065 6 CAACGCA 13375 0.0 15.051357 5 GTACTAC 660 0.0 15.004587 1 TCAACGC 13630 0.0 14.907801 4 TATCAAC 13635 0.0 14.876733 2 GTATCGA 200 0.0040071816 14.854542 1 AGTACTT 4680 0.0 14.848898 12-13 CTTACAC 1470 0.0 14.718343 3 ATCAACG 13780 0.0 14.6770115 3 ACACCGA 330 4.450818E-6 14.230336 6 CTACACT 1535 0.0 14.095325 4 CGCAGAG 14515 0.0 13.942667 8 ACGCAGA 14565 0.0 13.894802 7 GTACATG 15110 0.0 13.763308 1 CATGGGG 4125 0.0 13.569026 4 >>END_MODULE