FastQCFastQC Report
Wed 25 May 2016
SRR1295074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545141
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG2901.1405064E-920.8958235
TATACTG13450.018.397135
GTACATG103850.017.9711481
GTATATA21050.017.6126941
TACATGG108200.016.8392492
GTATAGG8600.016.8056471
GTATCAA79650.016.7253781
ACATGGG113200.015.5465593
TATACAG19600.015.4586445
GTATAGA13050.015.4086751
GTGTATA16100.015.221751
TCAACGC83250.015.2083244
TATCAAC86700.015.0728792
ATCAACG85550.014.9971053
CCTATAC9300.014.6503273
GTATTAT15950.014.5769581
GTATAAA21500.014.3213341
CAACGCA81600.014.2954595
GTATTAA19050.014.1840211
AACGCAG80850.014.1193916
Produced by FastQC (version 0.11.5)