##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295074_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3545141
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.743230805206338	27.0	22.0	33.0	6.0	33.0
2	21.766505196831382	27.0	2.0	33.0	2.0	33.0
3	24.09422699971595	27.0	15.0	33.0	6.0	33.0
4	23.893278997929844	33.0	2.0	37.0	2.0	37.0
5	25.910663919996413	33.0	15.0	37.0	2.0	37.0
6	26.822165606389138	33.0	15.0	37.0	2.0	37.0
7	26.59928279298341	33.0	15.0	37.0	6.0	37.0
8	27.276875306229005	33.0	15.0	37.0	6.0	37.0
9	28.300645587862373	33.0	22.0	37.0	15.0	37.0
10-11	27.87436691516642	33.0	18.5	37.0	6.0	37.0
12-13	26.655941470311056	33.0	15.0	37.0	4.0	37.0
14-15	28.298837761318943	33.0	15.0	37.0	4.0	40.0
16-17	28.76045931600464	33.0	22.0	37.0	6.0	40.0
18-19	28.959561410956574	33.0	22.0	37.0	6.0	40.0
20-21	28.451025784305898	33.0	18.5	37.0	6.0	40.0
22-23	29.235886527503418	33.0	22.0	37.0	6.0	40.0
24-25	29.364722869978937	33.0	24.5	37.0	6.0	40.0
26-27	27.859233807625706	33.0	15.0	37.0	4.0	40.0
28-29	28.606352328440536	33.0	22.0	37.0	6.0	40.0
30-31	29.351960472094056	33.0	22.0	37.0	6.0	40.0
32-33	28.082290803102048	33.0	18.5	37.0	4.0	40.0
34-35	27.705639916719814	33.0	15.0	37.0	6.0	40.0
36-37	27.99560920143938	33.0	22.0	37.0	6.0	40.0
38-39	28.11230441892156	33.0	18.5	37.0	6.0	40.0
40-41	27.19392190606805	33.0	15.0	37.0	6.0	40.0
42-43	27.805547508547612	33.0	15.0	37.0	6.0	40.0
44-45	27.67200049306925	33.0	18.5	37.0	6.0	40.0
46-47	27.398901905453126	33.0	15.0	37.0	6.0	40.0
48-49	27.34288805438204	33.0	15.0	37.0	6.0	40.0
50-51	27.238550032283626	33.0	18.5	37.0	6.0	40.0
52-53	27.509945725713024	33.0	18.5	37.0	6.0	40.0
54-55	27.44981821597505	33.0	22.0	37.0	6.0	40.0
56-57	27.431961662455738	33.0	22.0	37.0	6.0	40.0
58-59	27.292284707434767	33.0	22.0	37.0	6.0	40.0
60-61	27.279137416537168	33.0	22.0	37.0	6.0	40.0
62-63	27.270266824366082	33.0	22.0	37.0	6.0	40.0
64-65	26.815621014791795	33.0	18.5	37.0	6.0	40.0
66-67	27.020066056611007	33.0	22.0	37.0	6.0	37.0
68-69	26.590820506151942	30.0	18.5	37.0	6.0	37.0
70-71	26.74907189869176	27.0	22.0	37.0	6.0	37.0
72-73	26.783938777047233	27.0	22.0	37.0	6.0	37.0
74-75	26.9167896283956	27.0	22.0	37.0	6.0	37.0
76-77	26.671879059253214	27.0	22.0	33.0	6.0	37.0
78-79	26.26215233752339	27.0	22.0	33.0	6.0	37.0
80-81	26.097408255412127	27.0	22.0	33.0	6.0	37.0
82-83	26.1956006827373	27.0	22.0	33.0	6.0	37.0
84-85	26.14490396855866	27.0	22.0	33.0	6.0	37.0
86-87	25.968481084391286	27.0	22.0	33.0	6.0	37.0
88-89	25.921134589569217	27.0	22.0	33.0	6.0	37.0
90-91	26.061681608714576	27.0	22.0	33.0	6.0	37.0
92-93	25.969998936572622	27.0	22.0	33.0	6.0	37.0
94-95	25.846597779890843	27.0	22.0	33.0	6.0	37.0
96-97	25.923417573518233	27.0	22.0	33.0	6.0	37.0
98-99	26.01098602284084	27.0	22.0	33.0	6.0	37.0
100	25.666435834286986	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.321708374616378
1101	2	11.137808966251448
1101	3	8.303890380909959
1101	4	12.569067701265556
1101	5	10.621511668364072
1101	6	9.817057359771514
1101	7	9.265712525046887
1101	8	8.87046952888377
1101	9	7.271703690079217
1101	10-11	7.6867975025979085
1101	12-13	9.027213983523925
1101	14-15	7.9660520040553315
1101	16-17	8.062122797434334
1101	18-19	8.025403022352233
1101	20-21	7.132620311847742
1101	22-23	7.2961058183432215
1101	24-25	7.286299965196083
1101	26-27	8.446952196212997
1101	28-29	8.145324557361864
1101	30-31	5.603951588138127
1101	32-33	6.884357221746736
1101	34-35	8.662394045458939
1101	36-37	8.17244495039958
1101	38-39	8.139357494208465
1101	40-41	8.907252083866243
1101	42-43	8.596245488142156
1101	44-45	8.603187342304594
1101	46-47	7.396276755918169
1101	48-49	5.347900391859522
1101	50-51	6.845205856793189
1101	52-53	5.9618275634888
1101	54-55	4.14257534143859
1101	56-57	6.193748925170727
1101	58-59	6.228095263542638
1101	60-61	6.7741092766649835
1101	62-63	6.305241460181971
1101	64-65	6.7643742740141946
1101	66-67	5.854593102920141
1101	68-69	6.786967222335669
1101	70-71	6.331927675759932
1101	72-73	5.508342918060393
1101	74-75	4.911601163338283
1101	76-77	5.1558441316323
1101	78-79	5.251860196921079
1101	80-81	5.0231256062620595
1101	82-83	4.589492906604125
1101	84-85	4.429881550106117
1101	86-87	4.279394496685551
1101	88-89	4.16512395007156
1101	90-91	3.9298709205963434
1101	92-93	3.8488773922562665
1101	94-95	3.7835409403922995
1101	96-97	3.897368815855078
1101	98-99	3.6862049255863383
1101	100	3.78561254259499
1102	1	6.696258019697911
1102	2	10.270957408453029
1102	3	7.978378123715519
1102	4	12.303134436088168
1102	5	10.355589188502705
1102	6	9.315288205412795
1102	7	6.5905906237859995
1102	8	7.57621311751188
1102	9	6.392508836578248
1102	10-11	7.241649807211484
1102	12-13	8.863937984568015
1102	14-15	6.18771168292481
1102	16-17	7.227895279833419
1102	18-19	7.27068544355598
1102	20-21	6.844915028311757
1102	22-23	6.471153083404602
1102	24-25	6.999393112260659
1102	26-27	8.255252267269192
1102	28-29	7.503104758059376
1102	30-31	4.186817255667616
1102	32-33	4.361643708742729
1102	34-35	5.027265274223652
1102	36-37	5.115295509423355
1102	38-39	4.480855221135137
1102	40-41	6.066365947800868
1102	42-43	6.789279850702794
1102	44-45	6.360326315179982
1102	46-47	5.400064848929279
1102	48-49	3.6476257741491303
1102	50-51	4.965668501533944
1102	52-53	5.792711633123879
1102	54-55	5.341304758705245
1102	56-57	6.279290121452604
1102	58-59	5.971917407337184
1102	60-61	6.078453991678874
1102	62-63	4.28857696870509
1102	64-65	5.996987438262774
1102	66-67	3.6764467815286004
1102	68-69	6.014406017933084
1102	70-71	5.2235716841996656
1102	72-73	4.981341879407271
1102	74-75	4.21242737292507
1102	76-77	3.3346300947700804
1102	78-79	4.394933667581391
1102	80-81	4.453316062254348
1102	82-83	3.9241531510229493
1102	84-85	3.166528558495102
1102	86-87	3.2323781773241897
1102	88-89	3.4404184617051996
1102	90-91	3.280619503097668
1102	92-93	3.1972523895372795
1102	94-95	3.189437109520558
1102	96-97	3.4672635094795936
1102	98-99	3.188492953341761
1102	100	3.3922848466285167
1103	1	6.669802627354674
1103	2	10.81291520734354
1103	3	7.699565224326932
1103	4	11.556615809411358
1103	5	10.080488604785046
1103	6	8.82025091251722
1103	7	7.89999369236952
1103	8	7.932015048015888
1103	9	6.967026139966112
1103	10-11	7.394609764236481
1103	12-13	8.527862707977025
1103	14-15	8.354289562406205
1103	16-17	7.8715778187697225
1103	18-19	7.022830380686539
1103	20-21	8.153446943819883
1103	22-23	6.69342263145915
1103	24-25	5.08349408347129
1103	26-27	5.765264619120103
1103	28-29	6.766495573920221
1103	30-31	5.902362657261303
1103	32-33	7.777569451131608
1103	34-35	6.907770542096273
1103	36-37	3.93381092208606
1103	38-39	6.180189923130449
1103	40-41	6.5783119014148745
1103	42-43	7.288041959630316
1103	44-45	5.492819737229432
1103	46-47	6.210382739852335
1103	48-49	5.409327683725049
1103	50-51	6.2241951280773655
1103	52-53	6.007719792524984
1103	54-55	4.820994833573483
1103	56-57	5.764216326045066
1103	58-59	5.364253656479949
1103	60-61	4.956727444000702
1103	62-63	2.7119426034450846
1103	64-65	4.779195178774231
1103	66-67	3.9999153893592023
1103	68-69	4.950879602310479
1103	70-71	3.524383141673429
1103	72-73	4.33123822754342
1103	74-75	3.219875328555716
1103	76-77	3.557961244184657
1103	78-79	2.9400926066651856
1103	80-81	3.955794851045898
1103	82-83	3.2892346862677257
1103	84-85	3.177057689119593
1103	86-87	2.7913122682595777
1103	88-89	2.997179312032234
1103	90-91	2.5741040179110044
1103	92-93	2.2066253916547787
1103	94-95	2.732810410298285
1103	96-97	2.8369661512195243
1103	98-99	2.71103488535638
1103	100	2.879100072614907
1104	1	6.205786217261814
1104	2	9.452721729934105
1104	3	7.1000320419686815
1104	4	11.919948658432507
1104	5	9.302035749025258
1104	6	7.889092796157026
1104	7	3.8328830692069005
1104	8	6.3577528946994875
1104	9	6.330650692814725
1104	10-11	6.883160713896533
1104	12-13	7.218524691348538
1104	14-15	7.012679899430015
1104	16-17	6.931343992261624
1104	18-19	6.614132964845307
1104	20-21	7.383940218158653
1104	22-23	6.398476868383568
1104	24-25	5.895958430425363
1104	26-27	8.105489782553498
1104	28-29	7.039014781767062
1104	30-31	6.206501236658358
1104	32-33	7.425276210976573
1104	34-35	7.729794041963075
1104	36-37	6.4468353048799685
1104	38-39	7.1536946788161835
1104	40-41	6.998116887238584
1104	42-43	7.185921935210441
1104	44-45	6.524047586490546
1104	46-47	6.53465738534808
1104	48-49	5.31991011909491
1104	50-51	5.451466326170898
1104	52-53	5.877648814249881
1104	54-55	4.54735757109923
1104	56-57	5.775288899621806
1104	58-59	3.7247037131862086
1104	60-61	4.19477322457082
1104	62-63	3.7247137015828784
1104	64-65	2.6957909282996866
1104	66-67	3.0998145147821816
1104	68-69	3.9887911335932174
1104	70-71	2.4240021201364
1104	72-73	2.3320717899458003
1104	74-75	3.0089695077272793
1104	76-77	3.631552498831791
1104	78-79	4.172803613485055
1104	80-81	3.1063659715213525
1104	82-83	2.8618003101110574
1104	84-85	2.9026004820912163
1104	86-87	0.7503383684398628
1104	88-89	0.24735592307767718
1104	90-91	2.14664113396044
1104	92-93	2.4472688042348345
1104	94-95	2.3339894121180613
1104	96-97	2.7531673034578787
1104	98-99	2.376730593951411
1104	100	0.9536730020677524
1105	1	5.792083563080027
1105	2	-2.869465622584592
1105	3	0.4608218471031442
1105	4	8.125067001412006
1105	5	8.359425208370208
1105	6	7.961676047589311
1105	7	5.200699964403711
1105	8	5.798743828274208
1105	9	4.829133477919907
1105	10-11	4.9653393231675516
1105	12-13	5.103202580663169
1105	14-15	5.882235874375059
1105	16-17	6.03875341300305
1105	18-19	6.080979467312201
1105	20-21	6.9883162341207985
1105	22-23	1.9952602969739281
1105	24-25	3.9407404981647964
1105	26-27	6.79907103523372
1105	28-29	5.957729083513492
1105	30-31	5.352832686476187
1105	32-33	7.128992865892808
1105	34-35	6.0413920668606345
1105	36-37	6.158549698387503
1105	38-39	4.091538812438785
1105	40-41	5.341917190982716
1105	42-43	5.53480209959816
1105	44-45	6.13739916464548
1105	46-47	5.399266469799027
1105	48-49	4.940012243144963
1105	50-51	5.911233853095201
1105	52-53	5.576327924215786
1105	54-55	5.202095239743823
1105	56-57	5.088184306439707
1105	58-59	1.9847128486590897
1105	60-61	4.409813930907095
1105	62-63	-0.6700133167505093
1105	64-65	-0.3417048396774476
1105	66-67	0.9022924963803973
1105	68-69	2.935929943253946
1105	70-71	2.972219151802907
1105	72-73	2.7545314432702384
1105	74-75	2.799741453734306
1105	76-77	1.877031522820193
1105	78-79	3.1820971103077227
1105	80-81	1.6092309752620046
1105	82-83	0.9151195077351204
1105	84-85	1.3319310247839482
1105	86-87	-0.3137572257317949
1105	88-89	1.5037588090424308
1105	90-91	1.339161827468299
1105	92-93	1.6763105443620923
1105	94-95	0.976545131953447
1105	96-97	1.966538403978305
1105	98-99	1.9817422251884587
1105	100	1.9908632262482229
1106	1	5.922816835148932
1106	2	10.080441975804952
1106	3	6.5715693592047195
1106	4	11.47567606472126
1106	5	9.739875966355726
1106	6	9.258171973262915
1106	7	6.525275236688682
1106	8	7.365503191707337
1106	9	6.880642326092438
1106	10-11	5.567330454476345
1106	12-13	7.744739851647889
1106	14-15	7.6717832853554775
1106	16-17	7.559981681964288
1106	18-19	7.066346930494692
1106	20-21	8.12189960193049
1106	22-23	6.162721626736371
1106	24-25	4.938050453435505
1106	26-27	7.550517557481619
1106	28-29	6.15509355729905
1106	30-31	5.322529383808497
1106	32-33	7.222326973743957
1106	34-35	6.714096887526335
1106	36-37	5.915558097745102
1106	38-39	6.887114162531152
1106	40-41	6.613809196458249
1106	42-43	6.088132469280701
1106	44-45	4.282977228039442
1106	46-47	5.880144725698717
1106	48-49	5.701139046717078
1106	50-51	6.430446393228415
1106	52-53	5.572629503883618
1106	54-55	5.186604901337862
1106	56-57	4.740443066012098
1106	58-59	4.8556598410585
1106	60-61	4.856629841252495
1106	62-63	4.909921135145851
1106	64-65	5.138213634677513
1106	66-67	3.0514139076300815
1106	68-69	3.180901382820828
1106	70-71	2.803044459204333
1106	72-73	2.7883539034474722
1106	74-75	2.7567970015968406
1106	76-77	3.4284797751090217
1106	78-79	4.045681969544269
1106	80-81	3.663368169890223
1106	82-83	2.082142797535436
1106	84-85	2.6692848629962107
1106	86-87	3.2990790040776687
1106	88-89	3.4134887405854357
1106	90-91	3.2752497415695103
1106	92-93	2.8695388067801026
1106	94-95	1.5257860351564325
1106	96-97	1.598230408543479
1106	98-99	1.9675599613049037
1106	100	2.923792508789404
1107	1	5.976027495066429
1107	2	10.26453765487435
1107	3	6.037940499910611
1107	4	11.074762608731167
1107	5	9.312627460263933
1107	6	7.687857619304211
1107	7	3.414585143769365
1107	8	5.964913347135742
1107	9	5.379222732351543
1107	10-11	4.244189090617208
1107	12-13	5.923747737225085
1107	14-15	6.014953460747616
1107	16-17	6.799033411830308
1107	18-19	6.017020981480162
1107	20-21	5.420629099254921
1107	22-23	5.483685576599207
1107	24-25	4.316859913130582
1107	26-27	6.8176897582241125
1107	28-29	6.153399062465095
1107	30-31	4.862441313042137
1107	32-33	4.676406251172487
1107	34-35	6.428016837227304
1107	36-37	6.24044528962731
1107	38-39	6.140375069022809
1107	40-41	7.367470714152088
1107	42-43	5.847559291134299
1107	44-45	3.829779545052233
1107	46-47	3.224183818357748
1107	48-49	3.7364747033247703
1107	50-51	4.487744598839946
1107	52-53	3.699663846911225
1107	54-55	5.028713878575896
1107	56-57	4.964581942397285
1107	58-59	2.859032281705595
1107	60-61	2.729358753553427
1107	62-63	0.26970896296009883
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2206	78-79	1.165970724684911
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2206	94-95	2.51001810546634
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2206	98-99	1.3873701428666543
2206	100	1.6341777281079324
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2207	4	11.823984148917017
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2207	6	9.301685545076129
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2207	9	6.005038265800263
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2207	12-13	8.652503690590198
2207	14-15	8.18894561555572
2207	16-17	7.260234545594535
2207	18-19	4.1682378440660415
2207	20-21	7.402626978145246
2207	22-23	6.183715814026812
2207	24-25	4.314260371009599
2207	26-27	3.8442241435244
2207	28-29	4.994057978585257
2207	30-31	5.440427452449509
2207	32-33	6.68835802965372
2207	34-35	6.104212709388094
2207	36-37	6.727936792171924
2207	38-39	6.806404367012096
2207	40-41	4.126484636128168
2207	42-43	5.610501274829158
2207	44-45	4.0502563012120945
2207	46-47	5.504187499945122
2207	48-49	2.569111677283548
2207	50-51	5.412866451378651
2207	52-53	2.9155963998613714
2207	54-55	3.1013316722914084
2207	56-57	5.2079715578541865
2207	58-59	3.7810746423913777
2207	60-61	3.435803526113723
2207	62-63	4.162848422664698
2207	64-65	3.414090690144505
2207	66-67	3.5522876040411333
2207	68-69	3.5678017501360486
2207	70-71	3.6308429647961056
2207	72-73	3.7716958260995668
2207	74-75	3.4084779426149723
2207	76-77	3.959848764318597
2207	78-79	4.090883878960696
2207	80-81	4.229639924248001
2207	82-83	3.6681023890041047
2207	84-85	3.2875569947744303
2207	86-87	3.5082166758619984
2207	88-89	3.3358670061891473
2207	90-91	1.476008523554789
2207	92-93	-0.6303814507045082
2207	94-95	1.3531209086297729
2207	96-97	-0.8890185993253397
2207	98-99	0.797936384547075
2207	100	2.0433640657623258
2208	1	1.1165507801355012
2208	2	8.38580605169594
2208	3	6.393413669977427
2208	4	11.568650102741362
2208	5	10.003778419687627
2208	6	9.082342139642552
2208	7	7.490375424271495
2208	8	7.692464946461239
2208	9	6.474703218359249
2208	10-11	6.632172435735811
2208	12-13	6.916635294736963
2208	14-15	7.868948843742142
2208	16-17	7.18930155403104
2208	18-19	6.5145402749624814
2208	20-21	6.530919277012067
2208	22-23	5.778745181057971
2208	24-25	6.188486855803379
2208	26-27	8.002592040124227
2208	28-29	6.950069818050327
2208	30-31	5.960842490260443
2208	32-33	8.237662525648116
2208	34-35	6.406332338590779
2208	36-37	6.879707621556015
2208	38-39	7.221576915593378
2208	40-41	7.564794233308813
2208	42-43	7.036733249746682
2208	44-45	5.813449146886178
2208	46-47	4.48694954652083
2208	48-49	4.634169866102351
2208	50-51	5.9152072572118435
2208	52-53	5.626406869302333
2208	54-55	6.389180800333673
2208	56-57	6.412615275742901
2208	58-59	5.781981556918765
2208	60-61	5.090271608351511
2208	62-63	3.2131261330736542
2208	64-65	3.303394886492029
2208	66-67	3.0356801331481265
2208	68-69	5.598180442919507
2208	70-71	5.350121535819149
2208	72-73	4.625875659427287
2208	74-75	2.133595317380127
2208	76-77	3.368313938395584
2208	78-79	4.125679949352332
2208	80-81	3.6233745754275795
2208	82-83	1.4690555074237395
2208	84-85	2.013095926825052
2208	86-87	1.1583217496748652
2208	88-89	0.6796865403617076
2208	90-91	1.919669265575262
2208	92-93	0.8117005678161142
2208	94-95	-1.0103130907075233
2208	96-97	1.5322822794589328
2208	98-99	0.3127985072465371
2208	100	1.8414263332439837
2209	1	5.267716163128792
2209	2	9.543390282153844
2209	3	5.635322829781572
2209	4	-3.1546350812685127
2209	5	0.6829297337967439
2209	6	4.341439840513747
2209	7	2.1905001063377902
2209	8	5.143904223279957
2209	9	5.010164964154029
2209	10-11	5.0346464442017655
2209	12-13	7.182143774427676
2209	14-15	6.710382236773331
2209	16-17	6.168621591350174
2209	18-19	5.284173248737769
2209	20-21	6.013188087945199
2209	22-23	5.401121613046449
2209	24-25	5.219938282635763
2209	26-27	6.797905867166573
2209	28-29	6.272919148522245
2209	30-31	4.075394056465399
2209	32-33	6.1186280303438885
2209	34-35	5.584033674176659
2209	36-37	6.166456164482646
2209	38-39	6.73392647014094
2209	40-41	7.181436362694569
2209	42-43	6.856658821933767
2209	44-45	6.428254103958203
2209	46-47	6.713118398525641
2209	48-49	5.296315713919405
2209	50-51	4.634063690924332
2209	52-53	4.961072667287031
2209	54-55	5.475757185185078
2209	56-57	5.093639122560255
2209	58-59	3.2019571648228826
2209	60-61	4.1051749270130635
2209	62-63	4.278974446896296
2209	64-65	4.9217656535328445
2209	66-67	4.607923113793646
2209	68-69	3.8378055185282776
2209	70-71	3.830237283188911
2209	72-73	1.0898898563980381
2209	74-75	2.4904192841800032
2209	76-77	3.4339122253944083
2209	78-79	3.543966074029644
2209	80-81	3.7696540523507203
2209	82-83	3.182140558177032
2209	84-85	3.181290653229439
2209	86-87	1.626446959263614
2209	88-89	2.153453705771817
2209	90-91	2.480155699147627
2209	92-93	2.466184465658671
2209	94-95	0.9788053675752302
2209	96-97	0.4006179263940446
2209	98-99	0.49684361901599416
2209	100	0.4899805357459286
2210	1	1.5918521556967633
2210	2	-16.8740189907378
2210	3	-7.393897295741912
2210	4	-8.079285048178965
2210	5	-1.6915938316303993
2210	6	2.2275133185989375
2210	7	-8.48774457316437
2210	8	-8.640372146507906
2210	9	-3.7277148925367705
2210	10-11	-1.6122271516784572
2210	12-13	2.831159603410317
2210	14-15	-5.388492197009992
2210	16-17	-1.8299373238156882
2210	18-19	-0.8539546766609369
2210	20-21	1.703143551961638
2210	22-23	1.8822713160162863
2210	24-25	1.4790246974053467
2210	26-27	3.454688497945206
2210	28-29	2.77184979593763
2210	30-31	2.1915842596339523
2210	32-33	4.236855614923389
2210	34-35	1.9338008410188934
2210	36-37	3.664731517277385
2210	38-39	2.599955303559465
2210	40-41	3.521998971682333
2210	42-43	1.6450175414965926
2210	44-45	2.757644915061718
2210	46-47	2.351920614378745
2210	48-49	2.4865850786355637
2210	50-51	1.8523989969603
2210	52-53	1.8755000115611686
2210	54-55	2.2868585837317603
2210	56-57	2.4147928925162887
2210	58-59	1.1770181293101167
2210	60-61	2.20652716483324
2210	62-63	1.2340421303891738
2210	64-65	2.1711834544632183
2210	66-67	2.2909239119221425
2210	68-69	2.8140896800410786
2210	70-71	1.9831565995278098
2210	72-73	0.5916813541435673
2210	74-75	-0.12290479572601498
2210	76-77	-0.5324308842780177
2210	78-79	-0.22738225100916054
2210	80-81	0.4045505901148694
2210	82-83	0.496346215475846
2210	84-85	-1.4171714391380341
2210	86-87	-1.184194935470181
2210	88-89	-1.6965247197787505
2210	90-91	-2.0761372070872888
2210	92-93	-1.404965570568077
2210	94-95	-0.6511990838798134
2210	96-97	-0.17959499271896107
2210	98-99	-0.42828840392536094
2210	100	-0.27729942796263884
2211	1	1.1604129452040226
2211	2	-16.8740189907378
2211	3	-7.771207156901987
2211	4	-18.55512584712154
2211	5	-20.785880804754207
2211	6	-12.923439227474562
2211	7	-10.906690343548721
2211	8	-18.67325789143361
2211	9	-15.161696753974507
2211	10-11	-9.85032124968814
2211	12-13	-21.865124726153493
2211	14-15	-17.30153478802015
2211	16-17	-15.447145273263468
2211	18-19	-18.570547113045656
2211	20-21	-8.38231564687836
2211	22-23	-3.9395233032175554
2211	24-25	-1.9418539311828305
2211	26-27	-16.878338037599683
2211	28-29	-3.7575624602698063
2211	30-31	-4.465749980381116
2211	32-33	-0.7658647516399846
2211	34-35	-5.288084178582849
2211	36-37	-6.346181442883143
2211	38-39	-2.3376051334088537
2211	40-41	-5.076280014843736
2211	42-43	-1.5073466064823542
2211	44-45	0.09500411239129392
2211	46-47	0.6390820588949957
2211	48-49	0.7520295460398536
2211	50-51	-4.420473694062295
2211	52-53	-0.9576275937160545
2211	54-55	-0.5956961377351604
2211	56-57	-6.307115426149348
2211	58-59	-1.9398131787186124
2211	60-61	-0.8799399997156776
2211	62-63	-0.23191520565049117
2211	64-65	-3.4052925894392843
2211	66-67	-2.3653858120144804
2211	68-69	0.37092798742996536
2211	70-71	0.009566811523580299
2211	72-73	-1.0688741019669408
2211	74-75	-1.675334438176975
2211	76-77	-1.6473142298821877
2211	78-79	-1.0438187282806801
2211	80-81	-0.8727362290744374
2211	82-83	-1.3095897754076375
2211	84-85	-1.6455928906022308
2211	86-87	-1.9232835018000465
2211	88-89	-2.4154756641621162
2211	90-91	-2.1623257469686976
2211	92-93	-2.0329960155352076
2211	94-95	-2.1097068254356692
2211	96-97	-2.861700476097411
2211	98-99	-2.7889031199997554
2211	100	-2.2003472797407433
2212	1	-20.889716356566417
2212	2	-16.8740189907378
2212	3	-19.629174135219074
2212	4	-18.55512584712154
2212	5	-20.785880804754207
2212	6	-21.558211457432787
2212	7	-21.973131560974622
2212	8	-22.373078858213002
2212	9	-23.679102761533688
2212	10-11	-11.018815446645112
2212	12-13	-21.865124726153493
2212	14-15	-23.15820874863284
2212	16-17	-23.58599548213556
2212	18-19	-18.28848008242896
2212	20-21	-23.29399259137806
2212	22-23	-18.395095595152092
2212	24-25	-24.81712745415876
2212	26-27	-16.935148451095763
2212	28-29	-17.772461286210294
2212	30-31	-6.207243632207618
2212	32-33	-16.900355477469432
2212	34-35	-13.510844645811595
2212	36-37	-17.303685634939335
2212	38-39	-23.28886831224315
2212	40-41	-10.013551913495437
2212	42-43	-13.917154435929442
2212	44-45	-16.79284827501628
2212	46-47	-16.759967779538094
2212	48-49	-17.370683658575338
2212	50-51	-8.767060222387087
2212	52-53	-2.0856556785770657
2212	54-55	-23.10056981985173
2212	56-57	-23.037769892033573
2212	58-59	-12.248261573080924
2212	60-61	-6.243420500304932
2212	62-63	-3.251842122411251
2212	64-65	-17.01794131201498
2212	66-67	-17.285781438825136
2212	68-69	-14.253455158134626
2212	70-71	-9.417830733885278
2212	72-73	-17.20190080059038
2212	74-75	-6.2517050965362415
2212	76-77	-10.659262263992996
2212	78-79	-4.733060730172319
2212	80-81	-2.3942951674406387
2212	82-83	-2.9713917934403575
2212	84-85	-5.302795175155794
2212	86-87	-4.960536650063332
2212	88-89	-3.372249598468926
2212	90-91	-4.223563784816815
2212	92-93	-5.485352222119513
2212	94-95	-3.877794920052839
2212	96-97	-5.505137684075898
2212	98-99	-3.9600774590255057
2212	100	-3.9490704371727787
2213	1	-20.889716356566417
2213	2	-16.8740189907378
2213	3	-19.629174135219074
2213	4	-18.55512584712154
2213	5	-20.785880804754207
2213	6	-21.558211457432787
2213	7	-10.301743458991618
2213	8	-10.227894722235666
2213	9	-4.434768483913292
2213	10-11	-17.331978198763696
2213	12-13	-15.992780533518932
2213	14-15	-23.15820874863284
2213	16-17	-17.643715028877768
2213	18-19	-18.301630812062466
2213	20-21	-17.362689475230752
2213	22-23	-19.409633962033883
2213	24-25	-7.5683668309293
2213	26-27	-22.89484887905582
2213	28-29	-23.772282969519853
2213	30-31	-19.156016732553944
2213	32-33	-22.978814821264013
2213	34-35	-17.48979342598399
2213	36-37	-23.33703085605203
2213	38-39	-11.225128935472611
2213	40-41	-16.823342055461794
2213	42-43	-22.87774644734171
2213	44-45	-16.886909066358474
2213	46-47	-16.75757616375651
2213	48-49	-6.098374624132436
2213	50-51	-23.024014573314336
2213	52-53	-17.51881950160608
2213	54-55	-17.34242534393105
2213	56-57	-17.25820190336502
2213	58-59	-17.738497345126525
2213	60-61	-18.928945377756406
2213	62-63	-17.256199262959154
2213	64-65	-17.33204010047178
2213	66-67	-8.651407689062559
2213	68-69	-22.33137955185788
2213	70-71	-17.12198692718259
2213	72-73	-9.248651850284666
2213	74-75	-4.179479512283876
2213	76-77	-3.1848133282354993
2213	78-79	-4.737527234922716
2213	80-81	-3.4414061339676145
2213	82-83	-3.3666421036002667
2213	84-85	-3.3034809114370276
2213	86-87	-3.441273396031253
2213	88-89	-3.0478160191629584
2213	90-91	-3.068171397171483
2213	92-93	-3.7836439684309333
2213	94-95	-3.885744409450826
2213	96-97	-3.756506378332986
2213	98-99	-3.9071903956235694
2213	100	-3.682599632615954
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	985.0
3	1118.0
4	7433.0
5	22195.0
6	26647.0
7	32937.0
8	31650.0
9	33648.0
10	39713.0
11	48388.0
12	61305.0
13	80612.0
14	69055.0
15	74696.0
16	81388.0
17	83562.0
18	82901.0
19	81192.0
20	79980.0
21	78265.0
22	80677.0
23	85725.0
24	92628.0
25	100120.0
26	108200.0
27	114312.0
28	121756.0
29	126599.0
30	132140.0
31	142190.0
32	159957.0
33	191010.0
34	225827.0
35	260929.0
36	279279.0
37	234725.0
38	71281.0
39	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84610798539381	21.121917588712964	14.049827395156566	23.982147030736662
2	14.888095824354645	21.667841734430606	40.239966170283374	23.204096270931373
3	17.299429269788977	27.55421695476909	30.34890503368105	24.79744874176088
4	12.609061346261704	18.443074133502183	39.32688050939919	29.620984010836928
5	12.827509417224592	38.62040112993611	35.73301922958664	12.819070223252663
6	30.000145560499693	38.69605638634035	18.69499949053825	12.60879856262171
7	25.778082005092024	33.12301383270664	23.910202008313252	17.188702153888087
8	24.355798207629135	36.09738084842897	22.104122127267477	17.442698816674422
9	24.817908886836072	17.325130308672406	22.201025956434545	35.65593484805697
10-11	23.06374530099481	27.68891658167253	29.40347189658874	19.843866220743923
12-13	24.084101555170413	25.417263376544934	29.10357033018643	21.395064738098224
14-15	21.418210174540274	26.93189101157473	26.62391318474635	25.025985629138642
16-17	20.971701339175254	30.309586008795623	27.419329927399172	21.299382724629943
18-19	21.066589461669828	29.46416704350179	28.852942253163633	20.616301241664754
20-21	22.43105383528893	28.786273567868964	27.92509794965466	20.85757464718745
22-23	21.523045652344177	28.744397052410232	28.331954566384432	21.400602728861156
24-25	21.724849531454648	28.619267536653286	27.984944850245892	21.670938081646177
26-27	21.72780588313887	28.486179928539418	28.024293632291435	21.761720556030273
28-29	21.520660621732254	28.50431684104482	28.382297281898172	21.592725255324755
30-31	21.97320306166372	28.785762070962907	27.87323252571905	21.367802341654322
32-33	21.412165269398393	28.58549798790876	28.29032065094797	21.71201609174488
34-35	21.503016082974636	28.139238995793114	28.214016523480588	22.143728397751662
36-37	21.90398846130487	28.649889047486944	27.85664953624566	21.589472954962527
38-39	21.71562088022684	28.402279056244407	28.0385603211859	21.843539742342852
40-41	21.85244367020139	28.348496167186905	27.838126028722076	21.96093413388963
42-43	21.63720015406389	28.48264499990375	28.158828896164973	21.721325949867392
44-45	22.08460003241008	28.149598496196525	28.007033625535378	21.758767845858017
46-47	21.909163470523023	28.455274507212742	28.09685637492592	21.538705647338315
48-49	21.839442614413933	28.211010594584142	28.10952916923823	21.840017621763703
50-51	21.92513458453783	28.250220010774672	27.96917202637148	21.855473378316017
52-53	21.59978255923107	28.269890656368236	27.996328022308997	22.1339987620917
54-55	21.96251289028686	28.368237736494716	27.757469728243738	21.911779644974686
56-57	22.05526134626331	28.180097809274894	27.72651549242887	22.038125352032925
58-59	21.83662069275904	27.83370206001582	28.09384042591682	22.235836821308315
60-61	22.12601711290291	28.214352830614565	27.754252206946223	21.905377849536308
62-63	21.778205069128457	28.316783489664417	27.950769853542052	21.954241587665077
64-65	21.786476806069782	27.91992135233422	28.04470973321202	22.24889210838398
66-67	21.843917234647098	28.070304646690026	27.812578079151194	22.273200039511682
68-69	22.356569067010135	27.991489429115912	27.654542919243585	21.997398584630368
70-71	22.29152366809426	28.054891732862863	27.553900131101962	22.099684467940918
72-73	22.102002593079387	28.11302712940023	27.840996473107605	21.94397380441277
74-75	22.266777556289384	27.891120654776095	27.562292899972284	22.279808888962233
76-77	22.220769778814777	28.121452079582276	27.61328737135697	22.04449077024598
78-79	22.213593488923625	27.927975709229415	27.806708708384985	22.051722093461972
80-81	22.26181330121827	27.896368368049668	27.71285167663918	22.12896665409288
82-83	21.892671555893532	28.00860942078622	27.954533578777095	22.144185444543147
84-85	22.06715252301434	27.819025399263992	27.610788991445002	22.503033086276663
86-87	22.228984715113224	27.93643898930388	27.67403348652363	22.160542809059265
88-89	22.130647434114866	27.61636717350853	27.92360531381522	22.329380078561385
90-91	22.254881264400147	27.65822971075477	27.691932634900834	22.394956389944248
92-93	21.97869687515359	27.880164717423618	27.856354826021363	22.28478358140143
94-95	21.89277155226299	27.58078928058919	27.83571130481622	22.690727862331606
96-97	22.11972305924616	27.662086184071594	27.813928444104334	22.404262312577917
98-99	22.084640062422668	27.547091121174265	27.764346490013985	22.603922326389075
100	22.210046890636008	27.388734781545036	27.629630386449	22.771587941369962
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	475.0
1	536.0
2	531.0
3	441.5
4	483.5
5	690.0
6	1020.5
7	1503.0
8	2209.5
9	3202.0
10	4294.0
11	5402.0
12	6517.5
13	7709.0
14	8862.5
15	9846.5
16	10857.5
17	11977.5
18	13181.5
19	14285.0
20	15501.5
21	16987.5
22	18647.5
23	20701.0
24	23304.5
25	26919.0
26	31940.5
27	38485.5
28	46967.0
29	56675.5
30	66728.0
31	77132.5
32	87765.5
33	97917.0
34	106726.5
35	114347.0
36	120630.5
37	125954.0
38	130202.0
39	132597.5
40	132965.0
41	131547.0
42	128633.5
43	124901.0
44	120780.0
45	115827.5
46	110794.0
47	106213.5
48	101832.5
49	98763.5
50	97373.5
51	92843.0
52	84757.5
53	78039.5
54	72479.0
55	66882.5
56	61627.0
57	56199.0
58	50837.5
59	45406.0
60	39543.0
61	33716.0
62	28179.5
63	23055.5
64	18554.5
65	14728.0
66	11525.0
67	8908.5
68	6849.5
69	5398.5
70	4147.5
71	3067.0
72	2360.0
73	1810.5
74	1348.0
75	998.0
76	711.0
77	498.0
78	344.5
79	237.0
80	160.0
81	114.0
82	80.5
83	54.0
84	41.0
85	23.5
86	14.0
87	13.5
88	10.5
89	6.5
90	5.0
91	3.0
92	3.5
93	3.5
94	2.5
95	2.0
96	1.5
97	1.0
98	0.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.638302397563313
2	26.224316606871206
3	6.569357890137514
4	25.514923101789183
5	13.764671137198775
6	10.8583269325536
7	8.929433272188609
8	9.936840311852194
9	5.706655955292046
10-11	7.2403044053819015
12-13	11.079545214139578
14-15	8.410215559832459
16-17	8.683716670225529
18-19	7.149828455342115
20-21	8.663280247527531
22-23	6.18033246068351
24-25	5.637025438480444
26-27	10.589748052334166
28-29	6.802620826646952
30-31	4.455760715864334
32-33	9.464926782883953
34-35	7.694122744342185
36-37	7.574141620883344
38-39	6.6683666460657
40-41	8.9996702528898
42-43	6.9534469856064955
44-45	7.135273321991989
46-47	6.401705884194733
48-49	4.340588992088044
50-51	7.271981565754366
52-53	4.7295580062965055
54-55	5.452406547440567
56-57	5.349039713794176
58-59	4.107142706030592
60-61	4.461444551852804
62-63	3.2807439816921247
64-65	4.869524230488999
66-67	2.908798267826301
68-69	5.631778820644934
70-71	2.7271129695546663
72-73	1.7821717105187072
74-75	0.8613197613296621
76-77	1.277565546758225
78-79	1.8744952598500313
80-81	2.1891653956781973
82-83	0.9423179501182041
84-85	1.3854032886139085
86-87	0.4476690771960833
88-89	0.6933997829705504
90-91	0.31939491264240266
92-93	0.42580816954812234
94-95	0.6068729001187824
96-97	0.6227396879277862
98-99	0.44156212686604
100	0.6099334271894968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3545141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.45869671173588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.72917011820547	91.36911343373477
2	2.5101887656326736	4.742183186042068
3	0.40763763703875433	1.1551475977599708
4	0.1401137969407843	0.5293986660143716
5	0.06607894923362514	0.31208657123445954
6	0.03873637502324916	0.21953925000198923
7	0.023210755766189212	0.1534720417558049
8	0.016243987536447305	0.1227508713675595
9	0.012157390551377064	0.10335341402087847
>10	0.05387528238640919	0.8325706710629273
>50	0.0014664909245295456	0.0901814566813062
>100	9.403651307103635E-4	0.19366060905297539
>500	1.2005708992134654E-4	0.07096277883513717
>1k	6.0028539840769596E-5	0.10557945243571487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8207622771562543E-5	0.0
3	0.0	0.0	0.0	2.8207622771562543E-5	0.0
4	0.0	0.0	0.0	2.8207622771562543E-5	0.0
5	0.0	0.0	0.0	2.8207622771562543E-5	0.0
6	0.0	0.0	0.0	2.8207622771562543E-5	0.0
7	0.0	0.0	0.0	5.6415245543125086E-5	0.0
8	0.0	0.0	0.0	5.6415245543125086E-5	0.0
9	0.0	0.0	0.0	8.462286831468763E-5	0.0
10-11	0.0	0.0	0.0	1.1283049108625017E-4	2.8207622771562543E-5
12-13	2.8207622771562543E-5	0.0	0.0	1.1283049108625017E-4	2.8207622771562543E-5
14-15	2.8207622771562543E-5	2.8207622771562543E-5	0.0	1.1283049108625017E-4	2.8207622771562543E-5
16-17	2.8207622771562543E-5	2.8207622771562543E-5	0.0	1.2693430247203143E-4	2.8207622771562543E-5
18-19	2.8207622771562543E-5	2.8207622771562543E-5	0.0	1.410381138578127E-4	2.8207622771562543E-5
20-21	2.8207622771562543E-5	2.8207622771562543E-5	0.0	1.410381138578127E-4	2.8207622771562543E-5
22-23	2.8207622771562543E-5	2.8207622771562543E-5	0.0	1.974533594009378E-4	2.8207622771562543E-5
24-25	2.8207622771562543E-5	2.8207622771562543E-5	0.0	2.6797241632984414E-4	2.8207622771562543E-5
26-27	2.8207622771562543E-5	8.462286831468763E-5	0.0	2.961800391014067E-4	2.8207622771562543E-5
28-29	5.6415245543125086E-5	1.1283049108625017E-4	0.0	5.923600782028134E-4	2.8207622771562543E-5
30-31	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.002242506010339222	2.8207622771562543E-5
32-33	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.006826244710718135	2.8207622771562543E-5
34-35	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.0133281017595633	2.8207622771562543E-5
36-37	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.024441905131558944	2.8207622771562543E-5
38-39	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.04150751690835428	2.8207622771562543E-5
40-41	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.062310638702381654	2.8207622771562543E-5
42-43	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.08458055688053029	2.8207622771562543E-5
44-45	1.1283049108625017E-4	1.1283049108625017E-4	0.0	0.11149062900460094	2.8207622771562543E-5
46-47	1.1283049108625017E-4	1.410381138578127E-4	0.0	0.14282929790380694	4.231143415734381E-5
48-49	1.1283049108625017E-4	1.410381138578127E-4	0.0	0.17663613379552462	5.6415245543125086E-5
50-51	1.1283049108625017E-4	1.410381138578127E-4	0.0	0.21267137188619578	5.6415245543125086E-5
52-53	1.1283049108625017E-4	1.410381138578127E-4	0.0	0.24989133013327255	5.6415245543125086E-5
54-55	1.1283049108625017E-4	1.6924573662937525E-4	0.0	0.2938105987885954	5.6415245543125086E-5
56-57	1.1283049108625017E-4	1.6924573662937525E-4	0.0	0.3463190885778591	5.6415245543125086E-5
58-59	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.4048922172630087	5.6415245543125086E-5
60-61	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.46776700842082164	5.6415245543125086E-5
62-63	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.5320803883399842	5.6415245543125086E-5
64-65	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.6073383258945131	5.6415245543125086E-5
66-67	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.6978565873684572	5.6415245543125086E-5
68-69	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.7981487901327479	5.6415245543125086E-5
70-71	1.410381138578127E-4	1.6924573662937525E-4	0.0	0.9131794757951799	5.6415245543125086E-5
72-73	1.410381138578127E-4	1.6924573662937525E-4	0.0	1.0386328780717045	5.6415245543125086E-5
74-75	1.410381138578127E-4	1.8334954801515652E-4	0.0	1.1718292727990227	5.6415245543125086E-5
76-77	1.410381138578127E-4	1.974533594009378E-4	0.0	1.3218092030754207	5.6415245543125086E-5
78-79	1.5514192524359398E-4	1.974533594009378E-4	0.0	1.49428471251214	7.051905692890635E-5
80-81	1.6924573662937525E-4	1.974533594009378E-4	0.0	1.6873376827607138	8.462286831468763E-5
82-83	1.6924573662937525E-4	1.974533594009378E-4	0.0	1.8954253159465306	8.462286831468763E-5
84-85	1.6924573662937525E-4	1.974533594009378E-4	0.0	2.112017547397974	8.462286831468763E-5
86-87	1.6924573662937525E-4	1.974533594009378E-4	0.0	2.3529952687354325	8.462286831468763E-5
88	1.6924573662937525E-4	1.974533594009378E-4	0.0	2.551238441573974	8.462286831468763E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	290	1.1405064E-9	20.895823	5
TATACTG	1345	0.0	18.39713	5
GTACATG	10385	0.0	17.971148	1
GTATATA	2105	0.0	17.612694	1
TACATGG	10820	0.0	16.839249	2
GTATAGG	860	0.0	16.805647	1
GTATCAA	7965	0.0	16.725378	1
ACATGGG	11320	0.0	15.546559	3
TATACAG	1960	0.0	15.458644	5
GTATAGA	1305	0.0	15.408675	1
GTGTATA	1610	0.0	15.22175	1
TCAACGC	8325	0.0	15.208324	4
TATCAAC	8670	0.0	15.072879	2
ATCAACG	8555	0.0	14.997105	3
CCTATAC	930	0.0	14.650327	3
GTATTAT	1595	0.0	14.576958	1
GTATAAA	2150	0.0	14.321334	1
CAACGCA	8160	0.0	14.295459	5
GTATTAA	1905	0.0	14.184021	1
AACGCAG	8085	0.0	14.119391	6
>>END_MODULE