FastQCFastQC Report
Wed 25 May 2016
SRR1295074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545141
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62600.1765797185499815No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43590.12295702766124113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119900.019.8213371
TCAACGC131300.017.4762174
ATCAACG132150.017.3628253
AACGCAG131850.017.3436346
CAACGCA131600.017.3291875
TATCAAC136050.017.1244642
GAGTACT47400.016.68957912-13
GTACTAG7550.015.8379161
GTACATG149400.015.8074381
TAATACG3001.631628E-615.6736844
ACGCAGA145350.015.6017867
GTATACG2254.7080533E-415.5006271
CGCAGAG144950.015.3533228
AGTACTT57250.015.04819612-13
TACATGG154150.014.9199772
ACATGGG153800.014.4907153
GTATAAG13500.014.0243771
TAGGCGT4453.6603524E-813.7174896
CATGGGG61850.013.6843814
ATAGGCG5201.3842509E-913.5637655
Produced by FastQC (version 0.11.5)