FastQCFastQC Report
Wed 25 May 2016
SRR1295073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897560
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG8250.022.5017531
GTATTAG11400.017.3697761
TAGACGC2102.732669E-416.6482355
GTACATG97850.016.568741
GTATAAG11250.016.5012871
GTATATA21350.015.9409381
TACATGG101500.015.785252
ACATGGG100800.015.2349933
GTCCTCG3258.561139E-515.23195651
TACACTG16700.014.3553655
GTGTAGG10150.014.022031
TATAGGA15150.013.8830212
TAATACG2450.006761540713.7279854
CTATACT14700.013.7279854
TAATACT16350.013.713994
TATACCG2200.00712023513.6212845
GCGTGTC2250.0081297413.3509099
GTATTAT16300.013.2870791
GTGTAAG11650.013.2789321
CATGGGG37450.013.1720244
Produced by FastQC (version 0.11.5)