FastQCFastQC Report
Wed 25 May 2016
SRR1295073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897560
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59690.20600091111141788No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46580.16075594638247354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1802.3646862E-1028.7463155
CCGTGCG1458.72029E-725.9097659
GCCGCGT1101.0245574E-425.6153358
CCGCGTC1951.1404098E-519.2662379
GTATCAA103300.016.8965051
CTGTCGC5400.016.5234749
GAGTACT35150.016.29942512-13
GTGTCCG2355.665206E-515.98687659
CAACGCA107950.015.4691945
TCAACGC108350.015.4555014
ATCAACG109200.015.377213
AACGCAG109050.015.3770886
CCTATAC9950.015.1272123
TATCAAC114400.014.9849412
CATGGGG40950.014.9331514
GTACATG128150.014.8933091
GCCCGTG3203.2339412E-614.6754537
TACATGG129500.014.5347242
ACATGGG125150.014.2442733
CTTACAC14600.014.1752843
Produced by FastQC (version 0.11.5)