##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2897560 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.522999351178232 33.0 33.0 33.0 27.0 33.0 2 30.389866646419748 33.0 33.0 33.0 27.0 33.0 3 31.11667540965502 33.0 33.0 33.0 27.0 33.0 4 35.33261640828835 37.0 37.0 37.0 33.0 37.0 5 35.60782865583457 37.0 37.0 37.0 33.0 37.0 6 35.382600187744174 37.0 37.0 37.0 33.0 37.0 7 35.301873645412 37.0 37.0 37.0 33.0 37.0 8 35.29367191706125 37.0 37.0 37.0 33.0 37.0 9 35.28098192962354 37.0 37.0 37.0 33.0 37.0 10-11 35.252212378691034 37.0 37.0 37.0 33.0 37.0 12-13 35.183055916012094 37.0 37.0 37.0 33.0 37.0 14-15 36.67856575877635 40.0 37.0 40.0 33.0 40.0 16-17 36.66559657090794 40.0 37.0 40.0 33.0 40.0 18-19 36.69655882880768 40.0 37.0 40.0 33.0 40.0 20-21 36.70119307279228 40.0 37.0 40.0 33.0 40.0 22-23 36.6636456190726 40.0 37.0 40.0 33.0 40.0 24-25 36.63928184403429 40.0 37.0 40.0 33.0 40.0 26-27 36.62207063874432 40.0 37.0 40.0 33.0 40.0 28-29 36.56996938803683 40.0 37.0 40.0 33.0 40.0 30-31 36.48664859398943 40.0 37.0 40.0 33.0 40.0 32-33 36.450705075994975 40.0 37.0 40.0 33.0 40.0 34-35 36.35402114192631 38.5 37.0 40.0 33.0 40.0 36-37 36.351407563605235 37.0 37.0 40.0 33.0 40.0 38-39 36.28845442372203 37.0 37.0 40.0 33.0 40.0 40-41 36.238134326812904 37.0 37.0 40.0 33.0 40.0 42-43 36.157047481329116 37.0 37.0 40.0 27.0 40.0 44-45 36.060821863913084 37.0 37.0 40.0 27.0 40.0 46-47 35.9600080757603 37.0 37.0 40.0 27.0 40.0 48-49 35.8560323168459 37.0 37.0 40.0 27.0 40.0 50-51 35.762690850232616 37.0 37.0 40.0 27.0 40.0 52-53 35.671528630986074 37.0 37.0 40.0 27.0 40.0 54-55 35.56271328290009 37.0 37.0 40.0 27.0 40.0 56-57 35.443371664434906 37.0 35.0 40.0 27.0 40.0 58-59 35.32354826129571 37.0 33.0 40.0 27.0 40.0 60-61 35.17043685031544 37.0 33.0 40.0 27.0 40.0 62-63 34.984499371885306 37.0 33.0 40.0 27.0 40.0 64-65 34.81011178370767 37.0 33.0 40.0 27.0 40.0 66-67 34.600016565662145 37.0 33.0 40.0 27.0 40.0 68-69 34.37970947970016 37.0 33.0 38.5 27.0 40.0 70-71 34.15027281574842 37.0 33.0 37.0 27.0 40.0 72-73 33.92883736661191 37.0 33.0 37.0 27.0 40.0 74-75 33.69091390687337 37.0 33.0 37.0 27.0 40.0 76-77 33.45370415107884 37.0 33.0 37.0 27.0 38.5 78-79 33.20853752812711 37.0 33.0 37.0 27.0 37.0 80-81 32.98928771103964 37.0 33.0 37.0 27.0 37.0 82-83 32.77343644307624 37.0 33.0 37.0 22.0 37.0 84-85 32.59919587515013 37.0 33.0 37.0 22.0 37.0 86-87 32.61900202239126 37.0 33.0 37.0 22.0 37.0 88-89 32.627236019271386 37.0 33.0 37.0 22.0 37.0 90-91 32.62821580916357 37.0 33.0 37.0 22.0 37.0 92-93 32.59651568906253 37.0 33.0 37.0 22.0 37.0 94-95 32.621125360648264 37.0 33.0 37.0 24.5 37.0 96-97 32.585238269440495 37.0 33.0 37.0 22.0 37.0 98-99 32.50820863070997 33.0 33.0 37.0 22.0 37.0 100 31.980723091152555 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6840981506140835 1101 2 4.144777110420897 1101 3 2.3717539464855193 1101 4 1.2716865917601865 1101 5 0.5533105162904235 1101 6 0.6274873118228399 1101 7 0.6333769822674853 1101 8 0.6026526165202597 1101 9 0.739221994474903 1101 10-11 0.7126996345811065 1101 12-13 0.7771533566778643 1101 14-15 1.098755012703755 1101 16-17 1.1543236372098136 1101 18-19 1.194571569091032 1101 20-21 1.1473098101737236 1101 22-23 1.1175776585157706 1101 24-25 1.1137294792296757 1101 26-27 1.1570399390590467 1101 28-29 0.9825001294364242 1101 30-31 0.9007828940177447 1101 32-33 1.1375718712288077 1101 34-35 1.2704533364546862 1101 36-37 1.164849868047014 1101 38-39 1.1804818385062958 1101 40-41 1.1991561638465669 1101 42-43 1.2421058026486094 1101 44-45 1.3026057864432659 1101 46-47 1.3236351826517918 1101 48-49 1.1820284802229537 1101 50-51 1.1413554609080094 1101 52-53 1.1451516595912352 1101 54-55 1.2336591796265353 1101 56-57 1.24547057027673 1101 58-59 1.240033450600727 1101 60-61 1.1702352162361436 1101 62-63 1.1537970402172988 1101 64-65 1.141597444307017 1101 66-67 1.1248383716779387 1101 68-69 1.1023576365753058 1101 70-71 1.1395579603616355 1101 72-73 1.0976749248008844 1101 74-75 0.9937561373318644 1101 76-77 1.0940179143943354 1101 78-79 1.0777208214808454 1101 80-81 1.085948123542714 1101 82-83 1.1311175957105917 1101 84-85 1.0795387628598263 1101 86-87 1.1046685727728018 1101 88-89 1.1141685696278074 1101 90-91 1.074508642584263 1101 92-93 1.1100382334375567 1101 94-95 1.129245307714811 1101 96-97 1.101419112341631 1101 98-99 1.0534401300145007 1101 100 1.0906434675386727 1102 1 0.623383918754385 1102 2 4.089809949494992 1102 3 2.3296726025345293 1102 4 1.1041831942619567 1102 5 0.5112613398650083 1102 6 0.5750220534160704 1102 7 0.5826237187824574 1102 8 0.5538947077928142 1102 9 0.6722292618910473 1102 10-11 0.7096743986127052 1102 12-13 0.7060098756931694 1102 14-15 1.0199368901927315 1102 16-17 0.9803346832331243 1102 18-19 1.0285496930920672 1102 20-21 1.0034953869281367 1102 22-23 1.0113217985014842 1102 24-25 1.026037258598393 1102 26-27 0.989430623508234 1102 28-29 1.0447618637394882 1102 30-31 0.9204798396204268 1102 32-33 1.084024280875397 1102 34-35 1.1708783132772496 1102 36-37 1.0141319767379642 1102 38-39 1.0227511724780385 1102 40-41 1.0153691122849295 1102 42-43 1.060327333498968 1102 44-45 1.0703808676190647 1102 46-47 1.0405258856051702 1102 48-49 0.9243373011030585 1102 50-51 0.9049972933977912 1102 52-53 0.946541236185034 1102 54-55 0.9724487061997067 1102 56-57 0.9138458795684556 1102 58-59 0.9777739140488464 1102 60-61 1.0315027174334688 1102 62-63 1.0400537953069033 1102 64-65 1.0110331854873422 1102 66-67 0.9940176440088138 1102 68-69 0.9472086867723988 1102 70-71 0.9314295800333454 1102 72-73 0.9785138843651424 1102 74-75 0.9368891923592173 1102 76-77 0.8591674090362105 1102 78-79 0.8135793999681127 1102 80-81 0.8468202328433776 1102 82-83 0.8972519394479264 1102 84-85 0.862592716755799 1102 86-87 0.9605092260335226 1102 88-89 0.8740472857122157 1102 90-91 0.9341210168819032 1102 92-93 0.9205957484528824 1102 94-95 0.9642380400082189 1102 96-97 0.9788246786886674 1102 98-99 0.979663813305578 1102 100 0.9530650180477771 1103 1 0.6171745107857767 1103 2 4.019960188905202 1103 3 2.3187549898021516 1103 4 1.1083318018938044 1103 5 0.504961169581648 1103 6 0.4510206257345786 1103 7 0.473129362788292 1103 8 0.5670560406848679 1103 9 0.5529617753090648 1103 10-11 0.5925668707569187 1103 12-13 0.6407796378854371 1103 14-15 0.8733530237709459 1103 16-17 0.842772411515412 1103 18-19 0.8769120873077583 1103 20-21 0.8875519112960362 1103 22-23 0.7700859301913994 1103 24-25 0.8010924908178794 1103 26-27 0.8322665818481525 1103 28-29 0.776493973448332 1103 30-31 0.8360102707322028 1103 32-33 0.8402128232281356 1103 34-35 0.9329268299325193 1103 36-37 0.8489252554135973 1103 38-39 0.7944050103412152 1103 40-41 0.8671438880050957 1103 42-43 0.9021816113264265 1103 44-45 0.9823046739140011 1103 46-47 0.9380765214097622 1103 48-49 0.8667146419261869 1103 50-51 0.8520644523285554 1103 52-53 0.8910265523224083 1103 54-55 0.8076979888958888 1103 56-57 0.6855208571033486 1103 58-59 0.7894211332140983 1103 60-61 0.8405352569316449 1103 62-63 0.8900282856900787 1103 64-65 0.8483869056858921 1103 66-67 0.8401616806880483 1103 68-69 0.8254054610652872 1103 70-71 0.8097912861197045 1103 72-73 0.8519387234520082 1103 74-75 0.7759390691287607 1103 76-77 0.7295412466283224 1103 78-79 0.7521874389131469 1103 80-81 0.7251113446341435 1103 82-83 0.7618171632952482 1103 84-85 0.7128016245475592 1103 86-87 0.7635079027922202 1103 88-89 0.6943842054898894 1103 90-91 0.757648494867496 1103 92-93 0.7892116049656082 1103 94-95 0.7540299155179042 1103 96-97 0.7489635607261675 1103 98-99 0.7985822449168438 1103 100 0.836386163370026 1104 1 0.561084474178255 1104 2 3.630595821522853 1104 3 2.2453483506527228 1104 4 1.0594173841323524 1104 5 0.4536357516684717 1104 6 0.49561391656013143 1104 7 0.4314284940890829 1104 8 0.42708478984359743 1104 9 0.5270437668204764 1104 10-11 0.5259020843915678 1104 12-13 0.5193752278656376 1104 14-15 0.7149569272764751 1104 16-17 0.7039424711533897 1104 18-19 0.6534812311741476 1104 20-21 0.6992133131190243 1104 22-23 0.682577031179143 1104 24-25 0.7105462568020613 1104 26-27 0.6348013901157756 1104 28-29 0.6895705613627925 1104 30-31 0.6021734056742929 1104 32-33 0.7557485534917845 1104 34-35 0.8266415013401414 1104 36-37 0.7269436253090618 1104 38-39 0.7045741012998192 1104 40-41 0.710416234737842 1104 42-43 0.6920880890292977 1104 44-45 0.8057074227848489 1104 46-47 0.7949176735546715 1104 48-49 0.7235630883434396 1104 50-51 0.6546298965400155 1104 52-53 0.6398249425251308 1104 54-55 0.7289305458592921 1104 56-57 0.5848725421775143 1104 58-59 0.7368138685078733 1104 60-61 0.6653778951629619 1104 62-63 0.7764365276342886 1104 64-65 0.7536260411103797 1104 66-67 0.6541008406212896 1104 68-69 0.6844791468319187 1104 70-71 0.6552363534409764 1104 72-73 0.6737873060290056 1104 74-75 0.6773705943902826 1104 76-77 0.6572058929436437 1104 78-79 0.5656957504933402 1104 80-81 0.5593558136305674 1104 82-83 0.5750794147550806 1104 84-85 0.6769259617413539 1104 86-87 0.6875701511398304 1104 88-89 0.643382166983919 1104 90-91 0.6257422778132948 1104 92-93 0.7002671931562858 1104 94-95 0.7082709999719583 1104 96-97 0.6472283992212482 1104 98-99 0.7319743538757635 1104 100 0.627025958585758 1105 1 0.5157199565424335 1105 2 3.820894182836426 1105 3 2.2191245829102613 1105 4 1.0256007511686889 1105 5 0.36764522275748845 1105 6 0.41678864800553583 1105 7 0.3747603170472402 1105 8 0.37308544727299164 1105 9 0.4439182696567343 1105 10-11 0.43805830317879924 1105 12-13 0.4655417618701989 1105 14-15 0.6092255156278767 1105 16-17 0.588677350264561 1105 18-19 0.6647445353231802 1105 20-21 0.6254962847427805 1105 22-23 0.594435009169608 1105 24-25 0.7213615039756505 1105 26-27 0.6480560429964726 1105 28-29 0.5919651358391391 1105 30-31 0.5811030719127004 1105 32-33 0.6812062658550317 1105 34-35 0.7297760796495325 1105 36-37 0.6269381535396334 1105 38-39 0.642684737873175 1105 40-41 0.5616066905625345 1105 42-43 0.6630883111172565 1105 44-45 0.6348543333339478 1105 46-47 0.7039048237565524 1105 48-49 0.6407784341569638 1105 50-51 0.5713351524158341 1105 52-53 0.5122681925141137 1105 54-55 0.5666145912367355 1105 56-57 0.6029363007770456 1105 58-59 0.5514438530587427 1105 60-61 0.6030527307073186 1105 62-63 0.6440983754500564 1105 64-65 0.6108882332365368 1105 66-67 0.5962247762242541 1105 68-69 0.6395342804623354 1105 70-71 0.6264987378833595 1105 72-73 0.5449885307453073 1105 74-75 0.6073967618572738 1105 76-77 0.5944922022190866 1105 78-79 0.5375081460656546 1105 80-81 0.5440594988047209 1105 82-83 0.5880333501164969 1105 84-85 0.5142244554702131 1105 86-87 0.5827904392658922 1105 88-89 0.5539530024988508 1105 90-91 0.5272225459182494 1105 92-93 0.4822728951332351 1105 94-95 0.5197001653762214 1105 96-97 0.4814095929718789 1105 98-99 0.5102649337868002 1105 100 0.4188956547930225 1106 1 0.5158265253488317 1106 2 3.816237649780099 1106 3 2.1971029942496187 1106 4 1.0135499118158364 1106 5 0.403023534448927 1106 6 0.31737050177302706 1106 7 0.1756019102491564 1106 8 0.25238025022781585 1106 9 0.29309287526092476 1106 10-11 0.27501511659821887 1106 12-13 0.33616149659106753 1106 14-15 0.3816046981153036 1106 16-17 0.4386598854418864 1106 18-19 0.3468149579664228 1106 20-21 0.4037806898042362 1106 22-23 0.33963682499483383 1106 24-25 0.3458603569025769 1106 26-27 0.3165067959325256 1106 28-29 0.3713521544478269 1106 30-31 0.35842927619917475 1106 32-33 0.4512199584448169 1106 34-35 0.5272458983730886 1106 36-37 0.4136682047738418 1106 38-39 0.37146126715726524 1106 40-41 0.3241476334762794 1106 42-43 0.4158835205502811 1106 44-45 0.3654313864162617 1106 46-47 0.2737060762838368 1106 48-49 0.26538086461585664 1106 50-51 0.2745859205341574 1106 52-53 0.1951649516184446 1106 54-55 0.23044867822642345 1106 56-57 0.25102626778688375 1106 58-59 0.27533112565269136 1106 60-61 0.2815918483727131 1106 62-63 0.30547919539338153 1106 64-65 0.2162479565776536 1106 66-67 0.17055960925765135 1106 68-69 0.1569379371600519 1106 70-71 0.1919405740661304 1106 72-73 0.18081119439302995 1106 74-75 0.12136197961596906 1106 76-77 0.2411024689233514 1106 78-79 0.17760697926258473 1106 80-81 0.19200485445148985 1106 82-83 0.23701615669301646 1106 84-85 0.27096604954841297 1106 86-87 0.2728719913366717 1106 88-89 0.21254849910911133 1106 90-91 0.29372921125965945 1106 92-93 0.3169704275155212 1106 94-95 0.2846685212921045 1106 96-97 0.24440796504833884 1106 98-99 0.270089464386956 1106 100 0.20340414483665725 1107 1 0.5107139140545591 1107 2 3.6264013554968066 1107 3 2.128976774553358 1107 4 1.038236694623869 1107 5 0.3965342377011041 1107 6 0.28713420117648525 1107 7 0.19003712384878924 1107 8 0.16888942748874314 1107 9 0.2788591138820138 1107 10-11 0.20488987197544617 1107 12-13 0.23258535305766515 1107 14-15 0.22142325192697143 1107 16-17 0.26918041884162847 1107 18-19 0.2958485030545077 1107 20-21 0.2877699731923329 1107 22-23 0.25808819553480333 1107 24-25 0.28010570582316774 1107 26-27 0.25794784170443563 1107 28-29 0.17568182373684493 1107 30-31 0.2391567734827973 1107 32-33 0.3609170804369981 1107 34-35 0.5088027300826852 1107 36-37 0.38488861127483887 1107 38-39 0.3014856017404455 1107 40-41 0.24611056104806295 1107 42-43 0.24251406252137997 1107 44-45 0.3790742751342293 1107 46-47 0.21185669021600262 1107 48-49 0.22936798062163888 1107 50-51 0.24444387124186306 1107 52-53 0.18511761842454888 1107 54-55 0.18673106080096602 1107 56-57 0.2513664623019949 1107 58-59 0.2165827864528822 1107 60-61 0.23539707700854962 1107 62-63 0.3082800274991797 1107 64-65 0.2562366946404353 1107 66-67 0.22814382554450674 1107 68-69 0.21710412219310626 1107 70-71 0.20652170071470266 1107 72-73 0.16338847294378667 1107 74-75 0.24739485100109704 1107 76-77 0.207172223090744 1107 78-79 0.23538173629268755 1107 80-81 0.2580589746340749 1107 82-83 0.31897371800515373 1107 84-85 0.18397371131946016 1107 86-87 0.1974246549364409 1107 88-89 0.1966842796457442 1107 90-91 0.2224214383729901 1107 92-93 0.3077978890945019 1107 94-95 0.16783652337199584 1107 96-97 0.0438782488573608 1107 98-99 0.1612456124763355 1107 100 0.24404467590497703 1108 1 0.487551080812203 1108 2 3.6281728696379396 1108 3 2.0789296866280296 1108 4 1.0130594079702888 1108 5 0.3539070019467445 1108 6 0.11090425964911788 1108 7 0.0876806375178063 1108 8 0.1449458936567467 1108 9 0.08547072578898707 1108 10-11 0.1533079389853711 1108 12-13 0.0990628688806865 1108 14-15 0.008354815836227658 1108 16-17 -0.02489203509088611 1108 18-19 0.051025162688723924 1108 20-21 0.03489601146264221 1108 22-23 -0.05172133811031898 1108 24-25 0.059862139533692016 1108 26-27 0.1090497360406033 1108 28-29 0.0639276079536657 1108 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94-95 0.020975615939725856 1108 96-97 0.03481062666234891 1108 98-99 0.011568711869969661 1108 100 0.04623494063009659 1109 1 0.47910124212730665 1109 2 3.822062831522217 1109 3 2.175466167251926 1109 4 1.0293546109493121 1109 5 0.44658197945042133 1109 6 0.3986207181790675 1109 7 0.27783438068660615 1109 8 0.2437486502794428 1109 9 0.3452211446229825 1109 10-11 0.22086403347724826 1109 12-13 0.1999878701609603 1109 14-15 0.2785193865039446 1109 16-17 0.16135137147438172 1109 18-19 0.26364642336075406 1109 20-21 0.1564606582154866 1109 22-23 0.14505235984636755 1109 24-25 0.20218897270973457 1109 26-27 0.17711016884792485 1109 28-29 0.13350425640379626 1109 30-31 0.07204762133549991 1109 32-33 0.2013148337570314 1109 34-35 0.4130221894518442 1109 36-37 0.15099404759926216 1109 38-39 0.07913167170918456 1109 40-41 0.21758721395375602 1109 42-43 0.2759516723084232 1109 44-45 0.21163782521790608 1109 46-47 0.10366281514870934 1109 48-49 0.1147885017001542 1109 50-51 0.012799744516193812 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0.4379413335734412 2210 94-95 0.35547396765652906 2210 96-97 0.26753912745021324 2210 98-99 0.3027812678452051 2210 100 0.19389835545122835 2211 1 0.45422660727033914 2211 2 3.7882343286124396 2211 3 2.127673927763574 2211 4 0.9276043877326572 2211 5 0.5217123849695184 2211 6 0.30218202810080186 2211 7 0.3576729901188571 2211 8 0.24988312902875975 2211 9 0.17292479476479627 2211 10-11 0.26321901465731656 2211 12-13 0.1830219832745783 2211 14-15 0.1208401272345867 2211 16-17 0.061274799445513395 2211 18-19 0.24455751491672828 2211 20-21 0.22439715857248643 2211 22-23 0.062540361477474 2211 24-25 0.10184635977878997 2211 26-27 0.146399119585638 2211 28-29 0.17664817013075407 2211 30-31 0.16993987249630038 2211 32-33 0.26867870191595955 2211 34-35 0.4941349149991936 2211 36-37 0.08556134457821685 2211 38-39 0.08223330312353738 2211 40-41 -0.016946884758787917 2211 42-43 0.19273714783948037 2211 44-45 0.1579795164800899 2211 46-47 0.1804663033921301 2211 48-49 0.09385113955254809 2211 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0.31069036696271013 2212 9 0.23912529032281782 2212 10-11 0.288071611156262 2212 12-13 0.3024135714719449 2212 14-15 0.3822956636752508 2212 16-17 0.4271267831960728 2212 18-19 0.5121466760588262 2212 20-21 0.5066850234748657 2212 22-23 0.41739346711136704 2212 24-25 0.35543676606847896 2212 26-27 0.11834623028944691 2212 28-29 0.3140237223635296 2212 30-31 0.2713588852032345 2212 32-33 0.39106290944294386 2212 34-35 0.52434827827323 2212 36-37 0.5058083688183999 2212 38-39 0.39856275720581635 2212 40-41 0.3817952169502945 2212 42-43 0.32078793807051653 2212 44-45 0.5191155260768383 2212 46-47 0.3674118375640134 2212 48-49 0.44731774565894256 2212 50-51 0.3719596818266524 2212 52-53 0.3607829535495952 2212 54-55 0.1565770833443807 2212 56-57 0.420968266830549 2212 58-59 0.5246499409903649 2212 60-61 0.47757731979470464 2212 62-63 0.5859268962568365 2212 64-65 0.4342246208032279 2212 66-67 0.3875211143524453 2212 68-69 0.417656050196598 2212 70-71 0.19019131071478057 2212 72-73 0.3753081019019646 2212 74-75 0.49081323437675195 2212 76-77 0.5145582519362577 2212 78-79 0.4414295351983455 2212 80-81 0.4172640155576701 2212 82-83 0.4978385681796311 2212 84-85 0.45397224648237255 2212 86-87 0.5429156650287439 2212 88-89 0.48884937950194995 2212 90-91 0.5198778971505362 2212 92-93 0.6719326013529283 2212 94-95 0.6001039168993003 2212 96-97 0.34782440690998584 2212 98-99 0.5226341084654678 2212 100 0.32164618652410226 2213 1 -0.6663990922298986 2213 2 -26.026330235952123 2213 3 -14.907349564363239 2213 4 -6.40415541635187 2213 5 -2.1880047122014545 2213 6 -1.410202866018409 2213 7 -1.3015277235098992 2213 8 -1.0895891846132955 2213 9 -1.2043925594898255 2213 10-11 -1.1757367769276286 2213 12-13 -1.1811627734378476 2213 14-15 -1.400589512737497 2213 16-17 -1.4441335147727585 2213 18-19 -1.3314463025538785 2213 20-21 -1.4003389705455689 2213 22-23 -1.4610701955740453 2213 24-25 -1.2928317592997018 2213 26-27 -1.51775488681929 2213 28-29 -1.6143584730437937 2213 30-31 -1.7388106677118031 2213 32-33 -1.3735899505002962 2213 34-35 -1.533113043291202 2213 36-37 -1.6562191813351532 2213 38-39 -1.5813652585377511 2213 40-41 -1.3401305388926303 2213 42-43 -1.4275256426262644 2213 44-45 -1.4386976496158894 2213 46-47 -1.5784041907182242 2213 48-49 -1.5301390868362077 2213 50-51 -1.5159354166365233 2213 52-53 -1.6703691567519954 2213 54-55 -1.3900837186294908 2213 56-57 -1.3420283570381244 2213 58-59 -1.2795020639443138 2213 60-61 -1.401320981383435 2213 62-63 -1.4879278971691434 2213 64-65 -1.3876217531483164 2213 66-67 -1.443740094601111 2213 68-69 -1.5257388715574933 2213 70-71 -1.4018673995064646 2213 72-73 -1.5202868753892744 2213 74-75 -1.637912199219052 2213 76-77 -1.4468564689182664 2213 78-79 -1.2869552943816593 2213 80-81 -1.592800719236024 2213 82-83 -1.5440066368577803 2213 84-85 -1.4140205797873513 2213 86-87 -1.4167411571492323 2213 88-89 -1.3532481573270765 2213 90-91 -1.171602447530958 2213 92-93 -1.37615943444548 2213 94-95 -1.5713414865791258 2213 96-97 -1.4527100008731182 2213 98-99 -1.4793795244757462 2213 100 -1.4123564278922842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 25.0 5 41.0 6 44.0 7 30.0 8 47.0 9 76.0 10 349.0 11 1485.0 12 4411.0 13 7856.0 14 10618.0 15 12341.0 16 13181.0 17 13809.0 18 14452.0 19 15689.0 20 17618.0 21 19692.0 22 22497.0 23 23930.0 24 24921.0 25 25946.0 26 28709.0 27 33466.0 28 38900.0 29 47118.0 30 57394.0 31 71201.0 32 91206.0 33 123332.0 34 171210.0 35 247164.0 36 383178.0 37 665300.0 38 705987.0 39 4320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.29625700995523 21.17615963135243 10.61548493674738 29.912098421944965 2 12.755420897097933 23.308117497962723 36.68534291506077 27.251118689878574 3 15.058255911870678 27.85498833501291 29.280014909095932 27.80674084402049 4 10.344534813288535 19.41357465438663 38.48905133422034 31.7528391981045 5 11.178540984388578 39.32393371039676 35.759702607987464 13.737822697227195 6 28.733736130885145 40.69176111298924 18.215498877210745 12.359003878914871 7 25.35240540206496 33.706044697744396 23.83275528375065 17.108794616439994 8 24.027952808918933 37.261204774350816 21.30690430237095 17.403938114359306 9 24.59999765222393 16.98464071085135 22.237306299842768 36.17805533708195 10-11 22.398024678058984 28.162579781779613 29.838040311593467 19.601355228567936 12-13 23.509636473711623 25.567163398403963 29.912246917731895 21.010953210152515 14-15 21.065404688087224 27.234071350035226 26.9976316322368 24.702892329640747 16-17 20.26377955134518 30.654218513592475 27.938968720519856 21.143033214542488 18-19 20.292765408623957 29.697344101366994 29.864032516755145 20.145857973253907 20-21 21.44930302817954 28.998384128629024 29.154016601947813 20.398296241243624 22-23 20.771476682202888 28.928856710274413 29.234339908439615 21.065326699083084 24-25 20.8554095220014 28.952907774488907 29.053202046962777 21.138480656546914 26-27 20.736517890287537 28.788394175687877 29.20113744285216 21.273950491172428 28-29 20.573803538968907 28.760767165504202 29.54423227874886 21.121197016778034 30-31 21.122907151233296 29.160713317907273 28.95110481150251 20.765274719356924 32-33 20.338665128801395 28.97402367470422 29.52313061880786 21.16418057768653 34-35 20.45365949032247 28.732101346023992 29.642674901927013 21.171564261726523 36-37 21.015043212090067 29.04533740495532 28.967337865359678 20.972281517594936 38-39 20.877037155662258 29.03970692258882 29.133843320793893 20.949412600955032 40-41 20.880958835862018 28.848633864599105 29.144797116437786 21.12561018310109 42-43 20.749138696893787 28.872809466844572 29.188045368291206 21.19000646797043 44-45 21.133282827149777 28.610895875002374 29.054769296612232 21.201052001235617 46-47 21.03639115298913 29.07940601807019 28.97989241969058 20.904310409250105 48-49 21.020515705187343 28.879780463434244 29.003381110279356 21.09632272109905 50-51 21.0632607710729 28.84549632154646 28.768190546050786 21.323052361329854 52-53 20.820452487500617 28.923881062282707 28.722620543141037 21.53304590707564 54-55 21.09682094044489 29.03271573422699 28.472299173278127 21.398164152049993 56-57 21.175960343970015 28.681069740807487 28.60545998723557 21.53750992798693 58-59 20.84552414492599 28.43964687505339 29.110303308945667 21.60452567107495 60-61 21.23175648727741 28.822752502952394 28.570269828562022 21.37522118120818 62-63 20.75062727450651 28.955712642174454 28.782364309711543 21.511295773607493 64-65 20.69812649066024 28.56323782234462 28.975751265146503 21.76288442184863 66-67 20.955982228444775 28.670612832841268 28.72527303348408 21.648131905229878 68-69 21.224254910476027 28.68309003902973 28.660167925196582 21.43248712529766 70-71 21.135293905442172 28.65209761331337 28.55298676295367 21.659621718290783 72-73 20.978086035821995 28.753562272695028 28.79187973563712 21.476471955845856 74-75 21.269436777054075 28.443680467048786 28.550883932356356 21.735998823540783 76-77 21.051100060683005 28.745466896695987 28.64460470096534 21.558828341655673 78-79 21.221078333913216 28.66196133008599 28.731685360990994 21.385274975009803 80-81 21.12953427787658 28.55304361932819 28.628772758219885 21.688649344575346 82-83 20.728528376269477 28.74185040485016 28.752791601440858 21.776829617439507 84-85 20.874975774311675 28.490444939821057 28.601706213287187 22.032873072580074 86-87 21.08133341271752 28.62381473801924 28.427649511916687 21.86720233734655 88-89 20.815556371240778 28.370676817978573 28.828231360312728 21.985535450467918 90-91 20.965149747864732 28.343710907212394 28.710459389922562 21.980679955000312 92-93 20.633848534725274 28.66620672089149 28.699270356655855 22.000674387727386 94-95 20.52020263637118 28.329540703201427 28.904328223018037 22.24592843740935 96-97 20.80080275268936 28.36162179511515 28.731212446463022 22.10636300573247 98-99 20.782927016980285 28.285678491767317 28.709624760615654 22.221769730636744 100 20.69859967745457 28.24041380892431 28.683533738058287 22.37745277556283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 7.5 2 10.0 3 13.5 4 20.5 5 30.0 6 46.5 7 91.5 8 162.0 9 194.0 10 186.0 11 161.0 12 173.5 13 245.5 14 306.0 15 359.5 16 447.5 17 570.0 18 713.5 19 991.0 20 1406.5 21 1927.5 22 2793.5 23 4139.5 24 6123.0 25 9060.0 26 13572.0 27 20136.0 28 28765.0 29 39448.5 30 51338.0 31 63313.0 32 75437.5 33 86693.5 34 96823.0 35 106548.5 36 114450.0 37 120552.0 38 124550.5 39 126568.5 40 127123.0 41 125231.0 42 121417.5 43 116534.5 44 110765.5 45 105120.5 46 100822.5 47 96472.5 48 91628.0 49 89191.5 50 94097.0 51 88325.5 52 71537.5 53 62560.5 54 57182.5 55 52379.5 56 47638.0 57 42589.0 58 37440.5 59 32622.5 60 27710.5 61 22884.0 62 18472.5 63 14652.0 64 11360.5 65 8746.5 66 6696.0 67 5053.5 68 3768.0 69 2712.5 70 1926.0 71 1389.5 72 1020.5 73 713.0 74 522.0 75 371.0 76 213.5 77 122.0 78 75.0 79 38.0 80 21.0 81 11.5 82 6.0 83 4.5 84 5.5 85 2.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7656441971865984 2 4.7963113792294205 3 0.0 4 0.006039564323085631 5 0.16144618230511187 6 0.05684092822926877 7 0.035823244384930765 8 0.04313974516489736 9 0.0414831789505653 10-11 0.04217341487320366 12-13 0.04122434047957592 14-15 0.04353663082041442 16-17 0.034943193583566866 18-19 0.02892088515854719 20-21 0.023157415204516904 22-23 0.02326095059291266 24-25 0.020189400737171966 26-27 0.01240699070942448 28-29 0.019257582241610182 30-31 0.025228122972431977 32-33 0.0012769364568809619 34-35 0.003468435511257748 36-37 0.003692762186115214 38-39 0.005815237648228165 40-41 0.005918773036623918 42-43 0.007109430003175085 44-45 0.007765154129681525 46-47 0.016168776487803533 48-49 0.025210867074366018 50-51 0.021483593092118888 52-53 0.028196137439776912 54-55 0.02405472190394677 56-57 0.015426772870967297 58-59 0.01375295075856928 60-61 0.01144066041773078 62-63 0.017359433454354697 64-65 0.019447397120335733 66-67 0.019706235591325116 68-69 0.027402366128742806 70-71 0.027730228191996026 72-73 0.024451607559463826 74-75 0.025262634768563896 76-77 0.018567346318971823 78-79 0.014633001559933185 80-81 0.01354587998177777 82-83 0.008748740319441185 84-85 0.011233589640939273 86-87 0.008886787503968856 88-89 0.006919615124449537 90-91 0.007126685901241044 92-93 0.004037880147434393 94-95 0.001880892889189525 96-97 0.0010353538839575366 98-99 7.937713110341114E-4 100 0.10643437927083477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2897560.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.94689224038655 #Duplication Level Percentage of deduplicated Percentage of total 1 90.84888722252454 78.99028407495872 2 7.3045149672382905 12.702097514495167 3 1.159910075024245 3.025517289050152 4 0.32488055181017955 1.12989417316948 5 0.13128583534587013 0.5707447689253252 6 0.05914314952723071 0.3085387829220719 7 0.0447976780273298 0.27265132188432434 8 0.02430839922362715 0.16908318142664047 9 0.019106991676031457 0.14951641916644828 >10 0.07774584898878663 1.1283446030786288 >50 0.002780069601408896 0.16281214401249813 >100 0.0018829751822355824 0.3846431181958857 >500 2.786132009514139E-4 0.14541652473712482 >1k 4.3782074351920986E-4 0.653889869158426 >5k 3.9801885774473626E-5 0.20656621481908677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5969 0.20600091111141788 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4658 0.16075594638247354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.4511796131917887E-5 0.0 0.0 0.0 6 0.0 3.4511796131917887E-5 0.0 0.0 0.0 7 0.0 3.4511796131917887E-5 0.0 3.4511796131917887E-5 0.0 8 3.4511796131917887E-5 3.4511796131917887E-5 0.0 3.4511796131917887E-5 0.0 9 3.4511796131917887E-5 3.4511796131917887E-5 0.0 3.4511796131917887E-5 0.0 10-11 3.4511796131917887E-5 3.4511796131917887E-5 0.0 3.4511796131917887E-5 0.0 12-13 3.4511796131917887E-5 3.4511796131917887E-5 0.0 6.902359226383577E-5 0.0 14-15 3.4511796131917887E-5 3.4511796131917887E-5 0.0 1.0353538839575367E-4 0.0 16-17 5.176769419787683E-5 3.4511796131917887E-5 0.0 1.0353538839575367E-4 0.0 18-19 6.902359226383577E-5 3.4511796131917887E-5 0.0 1.0353538839575367E-4 0.0 20-21 6.902359226383577E-5 3.4511796131917887E-5 0.0 1.0353538839575367E-4 0.0 22-23 6.902359226383577E-5 3.4511796131917887E-5 0.0 1.0353538839575367E-4 0.0 24-25 6.902359226383577E-5 3.4511796131917887E-5 0.0 1.3804718452767157E-4 0.0 26-27 6.902359226383577E-5 3.4511796131917887E-5 0.0 2.5883847098938416E-4 0.0 28-29 6.902359226383577E-5 3.4511796131917887E-5 0.0 6.384682284404809E-4 0.0 30-31 6.902359226383577E-5 3.4511796131917887E-5 0.0 0.002743687792487472 0.0 32-33 8.627949032979473E-5 3.4511796131917887E-5 0.0 0.00867971672717735 0.0 34-35 1.0353538839575367E-4 3.4511796131917887E-5 0.0 0.016962547798837643 0.0 36-37 1.0353538839575367E-4 3.4511796131917887E-5 0.0 0.030525683678681373 0.0 38-39 1.0353538839575367E-4 3.4511796131917887E-5 0.0 0.051111970071370395 0.0 40-41 1.0353538839575367E-4 3.4511796131917887E-5 0.0 0.0767542345973854 0.0 42-43 1.3804718452767155E-4 3.4511796131917887E-5 0.0 0.10448446278938142 0.0 44-45 1.3804718452767155E-4 3.4511796131917887E-5 0.0 0.1371843896243736 0.0 46-47 1.3804718452767155E-4 3.4511796131917887E-5 0.0 0.1776667264871133 0.0 48-49 1.3804718452767155E-4 3.4511796131917887E-5 0.0 0.22027153881196593 0.0 50-51 1.3804718452767155E-4 3.4511796131917887E-5 0.0 0.26708679026491255 0.0 52-53 1.3804718452767155E-4 3.4511796131917887E-5 0.0 0.3181297367440191 0.0 54-55 1.553030825936305E-4 3.4511796131917887E-5 0.0 0.3756609008959262 0.0 56-57 1.7255898065958946E-4 3.4511796131917887E-5 0.0 0.4444256546887726 0.0 58-59 1.7255898065958946E-4 3.4511796131917887E-5 0.0 0.5232333411560071 0.0 60-61 1.7255898065958946E-4 5.176769419787683E-5 0.0 0.6049572743963887 0.0 62-63 1.7255898065958946E-4 6.902359226383577E-5 0.0 0.6923583981004708 0.0 64-65 1.7255898065958946E-4 6.902359226383577E-5 3.4511796131917887E-5 0.7883184472452684 0.0 66-67 1.7255898065958946E-4 6.902359226383577E-5 3.4511796131917887E-5 0.9017587211308825 0.0 68-69 1.7255898065958946E-4 6.902359226383577E-5 3.4511796131917887E-5 1.026777702618755 0.0 70-71 1.7255898065958946E-4 6.902359226383577E-5 3.4511796131917887E-5 1.171865293557338 0.0 72-73 1.7255898065958946E-4 6.902359226383577E-5 3.4511796131917887E-5 1.3230787283093361 0.0 74-75 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 1.484921796269965 0.0 76-77 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 1.6670750562542276 0.0 78-79 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 1.8677784066593963 0.0 80-81 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 2.092726293847237 0.0 82-83 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 2.337225113543809 0.0 84-85 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 2.591749610016704 0.0 86-87 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 2.8598372423694416 0.0 88 1.7255898065958946E-4 1.0353538839575367E-4 3.4511796131917887E-5 3.082938748464225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 180 2.3646862E-10 28.746315 5 CCGTGCG 145 8.72029E-7 25.909765 9 GCCGCGT 110 1.0245574E-4 25.615335 8 CCGCGTC 195 1.1404098E-5 19.266237 9 GTATCAA 10330 0.0 16.896505 1 CTGTCGC 540 0.0 16.523474 9 GAGTACT 3515 0.0 16.299425 12-13 GTGTCCG 235 5.665206E-5 15.9868765 9 CAACGCA 10795 0.0 15.469194 5 TCAACGC 10835 0.0 15.455501 4 ATCAACG 10920 0.0 15.37721 3 AACGCAG 10905 0.0 15.377088 6 CCTATAC 995 0.0 15.127212 3 TATCAAC 11440 0.0 14.984941 2 CATGGGG 4095 0.0 14.933151 4 GTACATG 12815 0.0 14.893309 1 GCCCGTG 320 3.2339412E-6 14.675453 7 TACATGG 12950 0.0 14.534724 2 ACATGGG 12515 0.0 14.244273 3 CTTACAC 1460 0.0 14.175284 3 >>END_MODULE