FastQCFastQC Report
Wed 25 May 2016
SRR1295072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429957
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46321.0773170340289844No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37410.87008700870087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20860.48516479554932235No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17090.3974816086259789No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17080.3972490272283042No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC14770.34352272436545983No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12960.30142549138634794No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12790.2974716076258789No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11020.2563047002374656No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10940.2544440490560684No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10750.25002500250025006No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8900.20699744393043953No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC7200.16745860632574885No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6740.1567598620327149No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC6670.15513179224899235No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6450.15001500150015No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT5830.13559495484432163No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5330.12396588496058909No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5270.12257039657454116No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5110.11884909421174675No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5020.11675586163267489No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA5000.11629069883732558No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4710.1095458383047607No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4570.10628969873731559No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTA701.4981169E-435.789945
CACCTAT700.002284146635.404211
TAATACT1151.0263848E-634.254974
GTACATA2251.2732926E-1030.2902681
CTATGCG1000.001189624825.1257279
CAGACTA1000.001189624825.1257279
CTGTGCG1100.00207201222.841579
TAGCAGT1502.4544843E-422.5104084
GTTTAAT1400.002490737522.127631
TACATAG2003.5865058E-521.681382
TGTACTG1905.1821044E-621.0972295
AGAGACG1454.2799916E-420.7334825
ACTATGC1705.145332E-520.6917748
GTCCTGG2707.4568015E-720.6524561
GTACAAG2156.252722E-520.1721671
GTACTGT2007.904895E-620.0922436
AGTACTC2258.18831E-620.0092533
GTACATC1650.00644948118.774961
CGATAGG650.00667562718.66778214-15
CATGGGG12750.018.5379834
Produced by FastQC (version 0.11.5)