##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295072_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 429957 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.88057177810804 27.0 22.0 33.0 6.0 33.0 2 22.0634226213319 27.0 6.0 33.0 2.0 33.0 3 24.443455973504328 27.0 15.0 33.0 15.0 33.0 4 24.247889905269595 33.0 6.0 37.0 2.0 37.0 5 26.41198538458497 33.0 15.0 37.0 2.0 37.0 6 27.292819979672387 33.0 15.0 37.0 6.0 37.0 7 27.122486667271378 33.0 15.0 37.0 6.0 37.0 8 27.84436583193203 33.0 15.0 37.0 6.0 37.0 9 28.686617498959198 33.0 22.0 37.0 15.0 37.0 10-11 28.4019901990199 33.0 18.5 37.0 6.0 37.0 12-13 27.21867070427973 33.0 15.0 37.0 4.0 37.0 14-15 28.87482701758548 33.0 22.0 37.0 6.0 40.0 16-17 29.290166225924917 33.0 22.0 37.0 6.0 40.0 18-19 29.435309810050775 33.0 22.0 37.0 6.0 40.0 20-21 28.934389950622972 33.0 22.0 37.0 6.0 40.0 22-23 29.779180243605754 33.0 27.0 37.0 15.0 40.0 24-25 29.951491660793984 33.0 27.0 37.0 15.0 40.0 26-27 28.458417934816737 33.0 18.5 37.0 4.0 40.0 28-29 29.166150335963827 33.0 22.0 37.0 6.0 40.0 30-31 29.809854241238078 33.0 27.0 37.0 15.0 40.0 32-33 28.543188039734204 33.0 22.0 37.0 6.0 40.0 34-35 28.16101726451715 33.0 22.0 37.0 6.0 40.0 36-37 28.466693180946002 33.0 22.0 37.0 6.0 40.0 38-39 28.505495898427053 33.0 22.0 37.0 6.0 40.0 40-41 27.49030600734492 33.0 18.5 37.0 6.0 40.0 42-43 28.03720837199999 33.0 22.0 37.0 6.0 40.0 44-45 27.83213902785627 33.0 22.0 37.0 6.0 40.0 46-47 27.401747151459332 33.0 22.0 37.0 6.0 40.0 48-49 27.229722972297232 33.0 18.5 37.0 6.0 40.0 50-51 27.230147433347987 33.0 22.0 37.0 6.0 40.0 52-53 27.476138311505572 33.0 22.0 37.0 6.0 40.0 54-55 27.3566612475201 33.0 22.0 37.0 6.0 40.0 56-57 27.199332723970073 33.0 22.0 37.0 6.0 40.0 58-59 27.046422084068872 33.0 22.0 37.0 6.0 38.5 60-61 26.927695095090904 33.0 22.0 37.0 6.0 37.0 62-63 26.674424419186103 27.0 22.0 37.0 6.0 37.0 64-65 26.307655184123064 27.0 18.5 37.0 6.0 37.0 66-67 26.48474382321953 27.0 22.0 37.0 6.0 37.0 68-69 25.987515030572823 27.0 18.5 33.0 6.0 37.0 70-71 25.94953797705352 27.0 18.5 33.0 6.0 37.0 72-73 25.855730921929403 27.0 22.0 33.0 6.0 37.0 74-75 25.90844549571236 27.0 22.0 33.0 6.0 37.0 76-77 25.598643585288762 27.0 22.0 33.0 6.0 37.0 78-79 25.198794298034457 27.0 18.5 33.0 6.0 37.0 80-81 25.101086852871333 27.0 18.5 33.0 6.0 37.0 82-83 25.258708428982434 27.0 22.0 33.0 6.0 37.0 84-85 25.11173908088483 27.0 22.0 33.0 6.0 37.0 86-87 24.772094651325595 27.0 15.0 33.0 6.0 37.0 88-89 24.689728275153097 27.0 15.0 33.0 6.0 37.0 90-91 24.833865944734008 27.0 18.5 33.0 6.0 37.0 92-93 24.65873796681994 27.0 15.0 33.0 6.0 37.0 94-95 24.600822872984974 27.0 15.0 33.0 6.0 37.0 96-97 24.758761922703897 27.0 15.0 33.0 6.0 37.0 98-99 24.78630653763051 27.0 15.0 33.0 6.0 37.0 100 24.322364794619 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.130091551959477 1101 2 11.104875746493807 1101 3 8.204265427857102 1101 4 12.556554581633431 1101 5 10.578048695479723 1101 6 9.955642465547147 1101 7 9.521385420841039 1101 8 9.142228794947783 1101 9 7.233955974927593 1101 10-11 7.774257976482023 1101 12-13 9.092231906116904 1101 14-15 7.931461144476021 1101 16-17 8.061161507590654 1101 18-19 8.026488401946544 1101 20-21 7.061405417612747 1101 22-23 7.127916243344963 1101 24-25 7.375954516489312 1101 26-27 8.455807685216758 1101 28-29 8.148244567377116 1101 30-31 5.159274090659661 1101 32-33 6.677238064641696 1101 34-35 8.604625041856181 1101 36-37 8.233509408822638 1101 38-39 8.121981063555086 1101 40-41 8.90604699758537 1101 42-43 8.558848944634509 1101 44-45 8.144800222018318 1101 46-47 7.077236968571647 1101 48-49 5.206314035325548 1101 50-51 6.567816866568375 1101 52-53 5.406469583927972 1101 54-55 4.203575672766576 1101 56-57 5.849252590094967 1101 58-59 5.799052787270131 1101 60-61 6.065605527207424 1101 62-63 5.85412713042723 1101 64-65 6.5110101225135075 1101 66-67 5.36888751040858 1101 68-69 6.483854215579541 1101 70-71 6.018001782558269 1101 72-73 5.321140266840786 1101 74-75 4.652993657314688 1101 76-77 4.781027309997299 1101 78-79 4.698378217322077 1101 80-81 4.861318239480351 1101 82-83 4.816485396649494 1101 84-85 4.1556273812938045 1101 86-87 3.981175927392048 1101 88-89 4.10174227951552 1101 90-91 3.962592233204667 1101 92-93 3.721889270599558 1101 94-95 3.5095238475448944 1101 96-97 3.8687009129746173 1101 98-99 3.661351639442991 1101 100 3.8532216964651305 1102 1 6.608690501849178 1102 2 10.256877277367135 1102 3 8.1635564528972 1102 4 12.291252298654076 1102 5 10.575437395398453 1102 6 9.193937304869952 1102 7 6.806948124294536 1102 8 8.072289169839742 1102 9 6.323187399112808 1102 10-11 7.262606030853821 1102 12-13 8.859071512734058 1102 14-15 5.777676103172542 1102 16-17 7.2281933334151205 1102 18-19 7.26452952911653 1102 20-21 6.589638419973557 1102 22-23 6.354717169609323 1102 24-25 6.900058246057956 1102 26-27 8.381660846083161 1102 28-29 7.606435814737861 1102 30-31 4.04196984678638 1102 32-33 4.588539328472233 1102 34-35 5.116871490961092 1102 36-37 5.117625307974205 1102 38-39 4.557885418373683 1102 40-41 6.186374737784128 1102 42-43 6.959996347095082 1102 44-45 6.305950978639565 1102 46-47 5.156126934901032 1102 48-49 3.501533388832584 1102 50-51 4.547374762391581 1102 52-53 5.867558632593401 1102 54-55 5.238537422434387 1102 56-57 6.268310887987436 1102 58-59 5.937796245581783 1102 60-61 5.675788629978051 1102 62-63 4.320273758850728 1102 64-65 5.883347757486224 1102 66-67 3.732020016955641 1102 68-69 5.579743971198834 1102 70-71 5.306919184201806 1102 72-73 4.95721974754791 1102 74-75 3.981177314811884 1102 76-77 3.2056167478313675 1102 78-79 4.0953872090527845 1102 80-81 3.885129555042365 1102 82-83 3.5892775633942797 1102 84-85 3.2234179784634094 1102 86-87 3.605162153832296 1102 88-89 3.4381957706377477 1102 90-91 3.270960541546639 1102 92-93 3.3767966656637824 1102 94-95 2.926932478918335 1102 96-97 3.044511609306973 1102 98-99 2.955830819995736 1102 100 3.0664925621395938 1103 1 6.443982469542362 1103 2 10.602545410487004 1103 3 7.602051746344802 1103 4 11.847572846488706 1103 5 10.135336627192231 1103 6 9.192915944880358 1103 7 8.160368951517643 1103 8 8.13419076051543 1103 9 7.0978491506947385 1103 10-11 7.381844484216575 1103 12-13 8.813305391839119 1103 14-15 8.457768440393995 1103 16-17 8.116914424779182 1103 18-19 7.3771089619982675 1103 20-21 8.291064960534221 1103 22-23 6.854217701030443 1103 24-25 5.15344185138024 1103 26-27 5.796746142535422 1103 28-29 6.845913900904318 1103 30-31 5.973238261537016 1103 32-33 7.710694992255327 1103 34-35 6.791297018009754 1103 36-37 4.102817269837455 1103 38-39 6.4413797476395125 1103 40-41 6.606269731662696 1103 42-43 7.120754037005259 1103 44-45 5.532506226254895 1103 46-47 6.126304008003949 1103 48-49 5.221930205687379 1103 50-51 6.040819447507754 1103 52-53 5.720891450201606 1103 54-55 4.4856157274476836 1103 56-57 5.594431917464487 1103 58-59 5.18695707110076 1103 60-61 4.667452942247376 1103 62-63 2.398477869404257 1103 64-65 4.5932994587064755 1103 66-67 3.846738027422621 1103 68-69 4.548435531693396 1103 70-71 3.1853431506213745 1103 72-73 4.103101009684266 1103 74-75 2.6043991842482157 1103 76-77 2.9109560725453676 1103 78-79 2.103373100606998 1103 80-81 3.593620408769265 1103 82-83 3.390003614688748 1103 84-85 3.262323833961716 1103 86-87 2.182596600683457 1103 88-89 2.6636539812060747 1103 90-91 2.4598499162635505 1103 92-93 1.952717010796011 1103 94-95 2.2294473116215876 1103 96-97 2.5913540261294763 1103 98-99 2.580863893672678 1103 100 2.401388766851081 1104 1 6.2591522423807895 1104 2 9.546110553377844 1104 3 7.207614759487498 1104 4 11.79635830037827 1104 5 9.247835196376094 1104 6 8.145645248341719 1104 7 4.113993165435932 1104 8 6.74818951874046 1104 9 6.634618523945239 1104 10-11 7.204716383681362 1104 12-13 7.297006228129472 1104 14-15 7.261694599875359 1104 16-17 7.279252222258862 1104 18-19 6.688973115978364 1104 20-21 7.5825092003285945 1104 22-23 6.334642401946503 1104 24-25 6.025582387008431 1104 26-27 8.194707272897514 1104 28-29 7.0525480316791835 1104 30-31 6.231704357264686 1104 32-33 7.517607655238294 1104 34-35 7.856420831299516 1104 36-37 6.338901932529055 1104 38-39 6.793554761600813 1104 40-41 6.951493580679511 1104 42-43 6.850673182886069 1104 44-45 6.576126173739603 1104 46-47 6.437238565075301 1104 48-49 5.39744712053561 1104 50-51 5.185085603589851 1104 52-53 5.591053123753532 1104 54-55 4.4451505127558875 1104 56-57 5.5528827511533585 1104 58-59 3.4857322924870395 1104 60-61 3.6420641938386176 1104 62-63 3.222504827714612 1104 64-65 2.593148572641063 1104 66-67 2.993331644421758 1104 68-69 3.7990295371807896 1104 70-71 1.8527902624539614 1104 72-73 2.1204442355936592 1104 74-75 2.721316807393791 1104 76-77 3.219681102635864 1104 78-79 3.502196189296786 1104 80-81 2.5365594612047744 1104 82-83 2.6585634678956858 1104 84-85 2.6831138525883382 1104 86-87 0.8266875901838695 1104 88-89 -0.07222950006497086 1104 90-91 1.6009769484560437 1104 92-93 1.9188058011753775 1104 94-95 1.7708958872224478 1104 96-97 2.2023801554336337 1104 98-99 2.203170057108302 1104 100 0.5674674981594201 1105 1 5.460934542344983 1105 2 -2.5321313790911404 1105 3 0.4249600919290053 1105 4 7.795014931754768 1105 5 8.425497667408653 1105 6 7.570351294964379 1105 7 4.830299160711057 1105 8 5.717450812383049 1105 9 4.702123641808104 1105 10-11 4.851128362070355 1105 12-13 4.817608300497966 1105 14-15 5.572331984934575 1105 16-17 5.629670434418777 1105 18-19 5.944271145629315 1105 20-21 6.723959835754343 1105 22-23 2.1049410058790983 1105 24-25 4.099100008446477 1105 26-27 6.612029859281858 1105 28-29 5.718609450537695 1105 30-31 5.117818677596734 1105 32-33 6.922298860621673 1105 34-35 5.903815394316233 1105 36-37 5.887827350234495 1105 38-39 3.8541048783714125 1105 40-41 4.811634606233916 1105 42-43 4.984119345671264 1105 44-45 5.506515919546786 1105 46-47 4.686949735191035 1105 48-49 4.763230744725703 1105 50-51 5.68419659308838 1105 52-53 5.345805022824095 1105 54-55 4.929558203175297 1105 56-57 4.7863946748166875 1105 58-59 2.3748283731837425 1105 60-61 4.238761961797305 1105 62-63 -0.5182452967130757 1105 64-65 -0.3578145314525507 1105 66-67 0.4970847438535557 1105 68-69 2.337228837683231 1105 70-71 2.4739501762441556 1105 72-73 2.3263213888683048 1105 74-75 2.3008908480619397 1105 76-77 1.58402451855315 1105 78-79 2.8288036711731337 1105 80-81 1.2804484289166318 1105 82-83 0.6502898711134648 1105 84-85 1.113696714672912 1105 86-87 -0.9844717155434104 1105 88-89 1.1984660338255253 1105 90-91 1.2333156485076202 1105 92-93 1.1135368234192171 1105 94-95 0.8038956867868912 1105 96-97 1.5041009734809414 1105 98-99 1.5746103554645074 1105 100 1.62948991541219 1106 1 5.734420972407612 1106 2 9.871835217986103 1106 3 6.611807691093933 1106 4 11.433845829458317 1106 5 9.833639629830365 1106 6 8.991019353419492 1106 7 6.355351352777863 1106 8 7.139162712174279 1106 9 6.530933662684934 1106 10-11 5.512401848500421 1106 12-13 7.6834746888069425 1106 14-15 7.520139918128731 1106 16-17 7.560776906235063 1106 18-19 6.733937116401755 1106 20-21 7.729179042434929 1106 22-23 5.694189440117093 1106 24-25 4.517680384229358 1106 26-27 7.312447395816303 1106 28-29 5.649194001685917 1106 30-31 4.581806151500697 1106 32-33 6.741192388805388 1106 34-35 6.366759026884083 1106 36-37 5.589810648773117 1106 38-39 6.86767481574093 1106 40-41 6.031363207486528 1106 42-43 5.48986047301991 1106 44-45 4.23094180681192 1106 46-47 5.615446603667028 1106 48-49 5.188596089193343 1106 50-51 6.102776968871261 1106 52-53 4.995596254556872 1106 54-55 4.81682333887467 1106 56-57 4.702887367927335 1106 58-59 4.645183279238022 1106 60-61 4.241371565137598 1106 62-63 4.627892490343292 1106 64-65 4.754920332848073 1106 66-67 2.3232851613900927 1106 68-69 2.8173958522970075 1106 70-71 2.6889814914842383 1106 72-73 2.387386107031144 1106 74-75 2.1448365683124635 1106 76-77 2.914400301434153 1106 78-79 3.660745216058313 1106 80-81 2.9918262994803086 1106 82-83 1.5626071988796433 1106 84-85 1.8835297000591353 1106 86-87 2.7582213951037744 1106 88-89 2.6833303344518313 1106 90-91 2.893545293601562 1106 92-93 2.7868144852146237 1106 94-95 1.3962756450332385 1106 96-97 1.4889652741072439 1106 98-99 1.718660459848639 1106 100 2.9739575563307703 1107 1 6.226598999775401 1107 2 9.88286620503153 1107 3 5.812580771838935 1107 4 11.409055737290316 1107 5 9.510857906626093 1107 6 7.950387031715799 1107 7 3.8960246988135445 1107 8 6.725894987383732 1107 9 5.717927126141365 1107 10-11 4.411527574403184 1107 12-13 6.175594598371081 1107 14-15 6.0537604977569615 1107 16-17 7.069567508928081 1107 18-19 6.445916677167908 1107 20-21 5.23254417722265 1107 22-23 5.471214218813614 1107 24-25 4.5756694170579415 1107 26-27 7.192391967385529 1107 28-29 6.243175048444538 1107 30-31 4.927599106255418 1107 32-33 4.6067351407175785 1107 34-35 6.465839626021662 1107 36-37 6.335342415280852 1107 38-39 6.176264399207216 1107 40-41 7.594148668463269 1107 42-43 6.064585154169684 1107 44-45 3.995553306388004 1107 46-47 3.19644914717561 1107 48-49 4.168431490456893 1107 50-51 4.864507648690026 1107 52-53 3.7477978937068457 1107 54-55 5.125591834011008 1107 56-57 4.922802857803443 1107 58-59 2.900093663863931 1107 60-61 2.6784425189988994 1107 62-63 0.07303042326395115 1107 64-65 1.861698692057125 1107 66-67 3.941473982715447 1107 68-69 4.76979851067372 1107 70-71 4.052755638861871 1107 72-73 2.4659670753877094 1107 74-75 2.4389160734678548 1107 76-77 2.6736376135199187 1107 78-79 3.5268725647013177 1107 80-81 2.003241319855725 1107 82-83 1.7279284193991025 1107 84-85 2.4393125354786314 1107 86-87 2.1001238757083804 1107 88-89 2.467367994788596 1107 90-91 1.5398209679960004 1107 92-93 2.1607034095566426 1107 94-95 2.350933502104464 1107 96-97 2.452555039903892 1107 98-99 1.8239357331162722 1107 100 0.384146177092731 1108 1 3.975691162496613 1108 2 -3.1361639029896136 1108 3 -0.0588372929737524 1108 4 5.943027238094729 1108 5 6.550097161969688 1108 6 6.385712244310604 1108 7 3.77209456780918 1108 8 4.89734752702979 1108 9 4.25575291331673 1108 10-11 3.0563816309419103 1108 12-13 2.115034956909348 1108 14-15 3.6167908356103275 1108 16-17 4.249935240484042 1108 18-19 3.884448049266279 1108 20-21 4.344859736864109 1108 22-23 3.8729873744100303 1108 24-25 2.624746640684691 1108 26-27 4.452628694574685 1108 28-29 5.943923152328704 1108 30-31 4.097770331261163 1108 32-33 4.121986807001193 1108 34-35 2.496159092810906 1108 36-37 0.507035129599398 1108 38-39 2.3627962309700266 1108 40-41 5.196378041021301 1108 42-43 2.345965296665664 1108 44-45 3.049715567937074 1108 46-47 4.169721958023693 1108 48-49 4.332461598478034 1108 50-51 1.730294824052013 1108 52-53 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52-53 -17.24364301743742 2213 54-55 -17.066583837779934 2213 56-57 -16.803097540548393 2213 58-59 -17.20735695282852 2213 60-61 -18.261866306342302 2213 62-63 -16.67674884053805 2213 64-65 -16.592636293090237 2213 66-67 -8.609969529351464 2213 68-69 -21.58500498277606 2213 70-71 -16.225204279551292 2213 72-73 -8.859081520798517 2213 74-75 -4.190303581293886 2213 76-77 -3.5464654651511474 2213 78-79 -4.852045930596489 2213 80-81 -3.5698257839484633 2213 82-83 -3.863726725636557 2213 84-85 -3.735888693799545 2213 86-87 -4.037141527913743 2213 88-89 -3.1047306075577126 2213 90-91 -3.1151751990653693 2213 92-93 -3.9190251362538184 2213 94-95 -4.298443303141184 2213 96-97 -3.9281704121775327 2213 98-99 -4.491248179492729 2213 100 -4.003856724989376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 107.0 3 92.0 4 699.0 5 2277.0 6 3299.0 7 3625.0 8 3352.0 9 3411.0 10 4235.0 11 5321.0 12 7018.0 13 8823.0 14 8260.0 15 9338.0 16 9834.0 17 9911.0 18 9977.0 19 9968.0 20 10220.0 21 10349.0 22 11030.0 23 11924.0 24 12732.0 25 13684.0 26 14592.0 27 14974.0 28 15786.0 29 16551.0 30 17914.0 31 20022.0 32 23079.0 33 26828.0 34 30021.0 35 31297.0 36 28332.0 37 17627.0 38 3445.0 39 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.6568607261787 20.314972099027106 12.362566989959669 23.665600184834528 2 15.460404224449167 23.99488354544534 33.86614397850353 26.67856825160196 3 21.37322587335921 29.1122206090057 26.48251306211542 23.03204045551967 4 14.441056009102892 22.098681976820657 29.475818908692712 33.98444310538374 5 13.61692799738009 40.11314206935894 31.58549436270432 14.68443557055665 6 31.74357211392918 32.80928509489823 18.19639494491849 17.2507478462541 7 25.085440358093397 34.60888982982498 20.516019437051376 19.78965037503024 8 27.21175473067999 33.91949593570235 19.94653709655309 18.922212237064567 9 26.72120858483581 19.451704169343095 18.698054829583064 35.12903241623803 10-11 23.600123713030484 30.21628664656951 23.633536168050988 22.55005347234902 12-13 26.010032354536655 28.12692874142012 23.595360841346803 22.267678062696415 14-15 25.57167608550493 27.895838631560355 21.91718354394699 24.61530173898773 16-17 22.339958405101097 30.999900605051025 25.593696095539936 21.06644489430794 18-19 23.8964652110192 29.788359788359784 26.051618352087836 20.263556648533175 20-21 23.44212268217025 29.080041024092218 25.431665313878348 22.046170979859184 22-23 22.513751206111372 29.61816203991076 23.040076699464702 24.82801005451316 24-25 23.122710454473637 30.538174095480247 21.737133534929693 24.60198191511642 26-27 24.230093192666125 27.78818380070693 22.95572931690763 25.025993689719318 28-29 24.067416063736665 27.223464073944026 25.24232593332575 23.466793928993557 30-31 25.397229701865463 28.69502462217694 23.940033476457316 21.967712199500276 32-33 21.83185329359528 28.702000081098067 25.37989376153113 24.08625286377552 34-35 20.39752297243308 29.39784758290052 25.56207550938873 24.642553935277668 36-37 24.408401292774624 28.46555815930199 23.768983116024906 23.35705743189848 38-39 25.44143788267825 26.79093422871815 23.695067153861345 24.07256073474225 40-41 23.10766471728959 29.00931819997793 23.506037742070905 24.37697934066157 42-43 25.169566663657644 27.944957146160593 23.77667776707445 23.108798423107316 44-45 26.282617914060673 26.867753034159875 23.359044003199514 23.490585048579938 46-47 23.285565985806485 28.020555106081936 26.545789047867093 22.14808986024449 48-49 24.61553695615159 27.4298003992257 25.92926242266964 22.025400221953067 50-51 22.838106179527685 27.890819595822542 25.73251593287148 23.53855829177829 52-53 21.870220111585358 28.55295934272437 23.337752074623314 26.239068471066957 54-55 22.826972022584766 29.26231386583713 22.326469813841456 25.584244297736646 56-57 24.227262538253125 26.756963530397833 22.94346821823285 26.07230571311619 58-59 24.061431192043354 25.860422315418706 25.181310674654707 24.896835817883233 60-61 25.049113777230055 27.53839090458758 24.067683980330326 23.344811337852043 62-63 21.91456179135133 27.84396054488373 25.211704395432093 25.02977326833285 64-65 20.636062987347916 28.606329075518065 25.519827664674295 25.23778027245972 66-67 23.92141788638011 27.545034886640728 23.728985008800503 24.804562218178656 68-69 25.483204897925294 26.178599905489776 23.32522140913037 25.012973787454563 70-71 23.19791299711514 27.724314726128473 23.60648284792109 25.4712894288353 72-73 25.141909086078325 26.896539508410626 23.60809829425692 24.353453111254126 74-75 25.91693116979929 25.985264401550918 23.27479101517631 24.823013413473486 76-77 23.37351152372518 27.286362985010115 25.95909670720506 23.381028784059648 78-79 24.583190094614345 26.887886316301806 25.540320075039368 22.988603514044485 80-81 23.059981617102025 27.256619325762742 25.24529308862336 24.438105968511874 82-83 22.260769922951464 27.702439870273516 23.537413531297453 26.49937667547757 84-85 22.972154358461026 28.146058758973407 22.505884248798154 26.37590263376741 86-87 24.029043453078803 26.234159921155758 23.36846604045905 26.36833058530639 88-89 24.029092969433183 25.36035503443464 25.195176457779954 25.41537553835222 90-91 24.955024595104764 26.68361915888123 24.10576769489245 24.25558855112156 92-93 22.316248428252496 26.958473206224937 25.01300555447986 25.71227281104271 94-95 21.104787380806517 27.55222533472383 25.43585414786305 25.907133136606603 96-97 24.11388802195657 26.696450175159292 24.064677436888303 25.124984365995832 98-99 24.8647385607552 25.69248833147377 23.704720568734775 25.73805253903626 100 22.95416450725795 26.98467169983985 25.065949509522405 24.99521428337979 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2242.0 1 2214.0 2 1866.0 3 1305.5 4 888.5 5 608.5 6 470.0 7 442.5 8 485.0 9 512.5 10 505.5 11 485.0 12 517.0 13 622.5 14 724.0 15 818.5 16 993.5 17 1158.5 18 1237.5 19 1324.0 20 1368.5 21 1437.5 22 1579.0 23 1722.5 24 1859.5 25 2124.0 26 2370.0 27 2629.0 28 3048.0 29 3433.5 30 3786.5 31 4182.5 32 4558.5 33 4934.5 34 5384.5 35 5894.5 36 6541.0 37 7217.5 38 7705.0 39 8238.0 40 8815.5 41 9134.0 42 9446.5 43 9877.0 44 10320.5 45 10846.0 46 12174.5 47 14468.0 48 17581.0 49 23177.5 50 30819.0 51 31032.0 52 23294.5 53 16860.5 54 13489.0 55 11894.0 56 10566.0 57 9343.5 58 8422.0 59 7662.0 60 6867.5 61 6074.0 62 5333.5 63 4609.0 64 3961.0 65 3283.5 66 2714.0 67 2215.0 68 1762.0 69 1370.5 70 1023.0 71 818.0 72 651.0 73 497.0 74 391.0 75 286.0 76 182.0 77 126.0 78 93.0 79 62.0 80 39.5 81 21.5 82 13.0 83 7.5 84 3.5 85 2.5 86 3.5 87 3.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.387482934339945 2 24.17683628827999 3 5.807324918538366 4 23.962396239623963 5 11.93561216586775 6 9.343027325988412 7 7.719144007423997 8 8.382000990796753 9 5.1342343536679245 10-11 6.375637563756376 12-13 9.748067830038817 14-15 7.174089501973453 16-17 7.5711059478040825 18-19 6.369706738115672 20-21 7.589014715425031 22-23 5.389957600411204 24-25 5.050621341203888 26-27 9.293603778982549 28-29 5.940826640803616 30-31 4.122970436578542 32-33 8.227101779945437 34-35 6.855801859255693 36-37 6.555422984158881 38-39 5.795230685859284 40-41 8.309784466818774 42-43 5.958153954930377 44-45 5.935593559355936 46-47 5.564858811462542 48-49 3.6993234207141645 50-51 6.064559944366529 52-53 4.20390876296932 54-55 4.597901650630179 56-57 4.543012440778962 58-59 3.198343090122966 60-61 3.749677293310726 62-63 2.841330644692376 64-65 4.179371425514645 66-67 2.2817398018871655 68-69 4.762685570882669 70-71 2.2869728833348453 72-73 1.519803143105008 74-75 0.7840318915612491 76-77 0.9931225680707605 78-79 1.561202631891096 80-81 1.9465900078379932 82-83 0.7472840307286542 84-85 1.1353460927488097 86-87 0.4119016552818072 88-89 0.6613452042878707 90-91 0.2585142235153748 92-93 0.30096032859099864 94-95 0.5328439820726258 96-97 0.512493109776094 98-99 0.4627206906737185 100 0.37189765488176724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 429957.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.15059076932516 #Duplication Level Percentage of deduplicated Percentage of total 1 94.73087231032673 75.92735379766195 2 3.3587337291912247 5.384089852630705 3 0.860556582676689 2.069223554759047 4 0.35360315836294626 1.1336600816275755 5 0.172816583303788 0.6925675623267452 6 0.10989653038132342 0.5284963100137299 7 0.07642508515581581 0.4287861007384235 8 0.048474422568395605 0.31082028848470533 9 0.038976895702425574 0.2811619095213403 >10 0.2102370371061733 3.2123411004568863 >50 0.01940528977360841 1.0796490167670572 >100 0.013732974190162341 2.383267761704263 >500 0.0029854291717744223 1.4960030166842422 >1k 0.003283972088951864 5.07257964662336 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4632 1.0773170340289844 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3741 0.87008700870087 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2086 0.48516479554932235 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1709 0.3974816086259789 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1708 0.3972490272283042 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1477 0.34352272436545983 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1296 0.30142549138634794 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1279 0.2974716076258789 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1102 0.2563047002374656 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1094 0.2544440490560684 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1075 0.25002500250025006 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 890 0.20699744393043953 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 720 0.16745860632574885 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 674 0.1567598620327149 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 667 0.15513179224899235 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 645 0.15001500150015 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 583 0.13559495484432163 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 533 0.12396588496058909 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 527 0.12257039657454116 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 511 0.11884909421174675 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 502 0.11675586163267489 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 500 0.11629069883732558 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 471 0.1095458383047607 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 457 0.10628969873731559 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.162906988373256E-4 0.0 22-23 0.0 0.0 0.0 3.488720965119768E-4 0.0 24-25 0.0 0.0 0.0 0.0010466162895359304 0.0 26-27 0.0 0.0 0.0 0.0022095232779091862 0.0 28-29 0.0 0.0 0.0 0.007326314026751513 0.0 30-31 0.0 0.0 0.0 0.018955383910484073 0.0 32-33 0.0 0.0 0.0 0.04279497717213582 0.0 34-35 0.0 0.0 0.0 0.08326414036752512 0.0 36-37 0.0 0.0 0.0 0.1322225245780392 0.0 38-39 0.0 0.0 0.0 0.19908967640950143 0.0 40-41 0.0 0.0 0.0 0.3099147124014727 0.0 42-43 0.0 0.0 0.0 0.38154978288526525 0.0 44-45 0.0 0.0 0.0 0.47051216749581937 0.0 46-47 0.0 0.0 0.0 0.5859888314412837 0.0 48-49 0.0 0.0 0.0 0.6837893091634746 0.0 50-51 0.0 0.0 0.0 0.7992659731089389 0.0 52-53 0.0 0.0 0.0 0.9353260907486097 0.0 54-55 0.0 0.0 0.0 1.0804568828975922 0.0 56-57 0.0 0.0 0.0 1.2280297797221582 0.0 58-59 0.0 0.0 0.0 1.3846733510560358 0.0 60-61 0.0 0.0 0.0 1.5281760734212955 0.0 62-63 0.0 0.0 0.0 1.6851685168516852 0.0 64-65 0.0 0.0 0.0 1.8778622048251337 0.0 66-67 0.0 0.0 0.0 2.0960235558439564 0.0 68-69 0.0 0.0 0.0 2.3739583260651647 0.0 70-71 0.0 0.0 0.0 2.7914419348911634 0.0 72-73 0.0 0.0 0.0 3.144616787260121 0.0 74-75 1.162906988373256E-4 0.0 0.0 3.4565084415418283 0.0 76-77 2.325813976746512E-4 0.0 0.0 3.832708852280577 0.0 78-79 2.325813976746512E-4 0.0 0.0 4.216119286347239 0.0 80-81 2.325813976746512E-4 0.0 0.0 4.629997883509281 0.0 82-83 2.325813976746512E-4 0.0 0.0 5.194938098461009 0.0 84-85 2.325813976746512E-4 0.0 0.0 5.701732963994074 0.0 86-87 2.325813976746512E-4 0.0 0.0 6.20457394576667 0.0 88 2.325813976746512E-4 0.0 0.0 6.65810767123224 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACTA 70 1.4981169E-4 35.78994 5 CACCTAT 70 0.0022841466 35.40421 1 TAATACT 115 1.0263848E-6 34.25497 4 GTACATA 225 1.2732926E-10 30.290268 1 CTATGCG 100 0.0011896248 25.125727 9 CAGACTA 100 0.0011896248 25.125727 9 CTGTGCG 110 0.002072012 22.84157 9 TAGCAGT 150 2.4544843E-4 22.510408 4 GTTTAAT 140 0.0024907375 22.12763 1 TACATAG 200 3.5865058E-5 21.68138 2 TGTACTG 190 5.1821044E-6 21.097229 5 AGAGACG 145 4.2799916E-4 20.733482 5 ACTATGC 170 5.145332E-5 20.691774 8 GTCCTGG 270 7.4568015E-7 20.652456 1 GTACAAG 215 6.252722E-5 20.172167 1 GTACTGT 200 7.904895E-6 20.092243 6 AGTACTC 225 8.18831E-6 20.009253 3 GTACATC 165 0.006449481 18.77496 1 CGATAGG 65 0.006675627 18.667782 14-15 CATGGGG 1275 0.0 18.537983 4 >>END_MODULE