FastQCFastQC Report
Wed 25 May 2016
SRR1295070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93975
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23022.449587656291567No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17171.8270816706570898No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7660.8151104017025805No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7360.7831870178238893No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6810.7246608140462889No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5080.5405693003458367No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5030.5352487363660549No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4780.5086459164671455No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4480.47672253258845443No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4260.45331205107741424No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3060.32561851556264965No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2790.29688747007182764No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2700.28731045490822027No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2590.27560521415270023No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2330.24793828145783453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.24368183027400903No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2270.2415536046820963No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC2230.2372971534982708No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2150.22878425113061984No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2070.22027134876296886No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2060.2192072359670125No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1980.21069433359936154No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1970.20963022080340518No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1950.20750199521149243No Hit
CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1710.1819632881085395No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1680.1787709497206704No Hit
CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1680.1787709497206704No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1640.17451449853684492No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1610.1713221601489758No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1340.14259111465815377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1270.13514232508645918No Hit
GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1140.12130885873902633No Hit
GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1130.12024474594306996No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1070.11386006916733174No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1050.11173184357541899No Hit
NNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGNN1000.10641127959563715No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG950.10109071561585528No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA300.00379531461.11061
ATACTAC250.004152490859.7509047
GATACTA300.00841669649.978796
TGTACTG300.00858408449.7306025
AATTGTG150.00964637148.306336-37
AAAAGTA700.002429891434.9203452
ACAGCAG300.005938142529.12250182-83
GACTCTG1201.2285815E-424.8962087
CATGGAC1150.00149070124.1393243
AGTACTC1100.002186647822.6048185
AAAGTAC1250.002421095722.2081783
ATGGACT1350.00376840620.5773894
GGGACGC650.00683914218.57934836-37
TGGGACG650.00683914218.57934836-37
GGACTCT1350.006956157718.5106646
ATGTACC1901.7357377E-518.31327494
TGGACTC1400.00882912117.760935
CATGGGC3008.4511715E-411.59840794
TGCCGAC3552.7699588E-411.02665594
TACATGG58350.010.8920352
Produced by FastQC (version 0.11.5)