##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295070_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93975 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.734078212290502 27.0 22.0 33.0 6.0 33.0 2 22.125426975259376 27.0 6.0 33.0 2.0 33.0 3 24.453226922053737 27.0 15.0 33.0 15.0 33.0 4 24.403607342378294 33.0 6.0 37.0 2.0 37.0 5 26.566427241287577 33.0 15.0 37.0 2.0 37.0 6 27.151167863793564 33.0 15.0 37.0 6.0 37.0 7 26.827368981112 33.0 15.0 37.0 6.0 37.0 8 27.571428571428573 33.0 15.0 37.0 6.0 37.0 9 28.350582601755786 33.0 22.0 37.0 15.0 37.0 10-11 28.094333599361534 33.0 18.5 37.0 6.0 37.0 12-13 26.939090183559458 33.0 15.0 37.0 4.0 37.0 14-15 28.461489757914336 33.0 15.0 37.0 6.0 40.0 16-17 28.852801276935356 33.0 22.0 37.0 6.0 40.0 18-19 28.936977919659483 33.0 22.0 37.0 6.0 40.0 20-21 28.547677573822824 33.0 18.5 37.0 6.0 40.0 22-23 29.376195796754455 33.0 24.5 37.0 6.0 40.0 24-25 29.52142591114658 33.0 24.5 37.0 6.0 40.0 26-27 28.088475658419796 33.0 18.5 37.0 6.0 40.0 28-29 28.70533120510774 33.0 22.0 37.0 6.0 40.0 30-31 29.232029795158287 33.0 22.0 37.0 6.0 40.0 32-33 27.90143123171056 33.0 22.0 37.0 6.0 40.0 34-35 27.61998935887204 33.0 15.0 37.0 6.0 40.0 36-37 27.920532056397978 33.0 22.0 37.0 6.0 40.0 38-39 27.92438414471934 33.0 18.5 37.0 6.0 40.0 40-41 26.97881883479649 33.0 15.0 37.0 6.0 40.0 42-43 27.35308858739026 33.0 15.0 37.0 6.0 40.0 44-45 27.079154030327217 33.0 15.0 37.0 6.0 40.0 46-47 26.54546953977122 33.0 15.0 37.0 6.0 40.0 48-49 26.292620377760045 30.0 15.0 37.0 6.0 40.0 50-51 26.447443469007716 33.0 15.0 37.0 6.0 40.0 52-53 26.72801276935355 33.0 15.0 37.0 6.0 40.0 54-55 26.535222133546156 33.0 15.0 37.0 6.0 40.0 56-57 26.284118116520354 27.0 15.0 37.0 6.0 38.5 58-59 26.099904229848363 27.0 15.0 37.0 6.0 37.0 60-61 25.936387337057727 27.0 15.0 37.0 6.0 37.0 62-63 25.503043362596436 27.0 15.0 35.0 6.0 37.0 64-65 25.318132482043097 27.0 15.0 33.0 6.0 37.0 66-67 25.49930300611865 27.0 15.0 33.0 6.0 37.0 68-69 24.96535248736366 27.0 15.0 33.0 6.0 37.0 70-71 24.755738228252195 27.0 15.0 33.0 6.0 37.0 72-73 24.473077946262304 27.0 15.0 33.0 6.0 37.0 74-75 24.459361532322426 27.0 15.0 33.0 6.0 37.0 76-77 24.0487895716946 27.0 15.0 33.0 6.0 37.0 78-79 23.72196328810854 27.0 15.0 33.0 6.0 37.0 80-81 23.76627294493216 27.0 15.0 33.0 6.0 37.0 82-83 24.0015003990423 27.0 15.0 33.0 6.0 37.0 84-85 23.692583133812185 27.0 15.0 33.0 6.0 33.0 86-87 23.20851822293163 27.0 15.0 33.0 6.0 33.0 88-89 23.094727321096038 27.0 15.0 33.0 6.0 33.0 90-91 23.259627560521416 27.0 15.0 33.0 6.0 33.0 92-93 22.966283586060122 27.0 15.0 33.0 6.0 33.0 94-95 23.005767491354085 27.0 15.0 33.0 6.0 33.0 96-97 23.209188613993085 27.0 15.0 33.0 6.0 33.0 98-99 23.21898909284384 27.0 15.0 33.0 6.0 33.0 100 22.54958233572759 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.335863496343514 1101 2 10.937596782075659 1101 3 8.05983128512634 1101 4 12.09688220043282 1101 5 10.678427947417322 1101 6 9.599738990006017 1101 7 9.07319517452633 1101 8 8.857904014096036 1101 9 7.205632266957856 1101 10-11 7.489574503792369 1101 12-13 9.300288700332164 1101 14-15 7.307610925886149 1101 16-17 7.881268266647439 1101 18-19 7.73802175547873 1101 20-21 6.212979567239145 1101 22-23 6.240450588134497 1101 24-25 6.506150300067585 1101 26-27 7.677526540547742 1101 28-29 7.403582187771029 1101 30-31 4.8971149326209655 1101 32-33 6.124691334899573 1101 34-35 7.63050170564032 1101 36-37 6.983121490871575 1101 38-39 8.065189823195013 1101 40-41 8.696961246773864 1101 42-43 8.602573360927906 1101 44-45 7.974780914961766 1101 46-47 6.4948190969898505 1101 48-49 5.393570729300794 1101 50-51 6.667533305602674 1101 52-53 5.796090956435052 1101 54-55 3.6356214765154284 1101 56-57 6.138010318050618 1101 58-59 5.5291806629863345 1101 60-61 6.227375311100648 1101 62-63 5.339176023161613 1101 64-65 6.465344766025485 1101 66-67 6.253043054772025 1101 68-69 6.1010168695958775 1101 70-71 5.663811407324726 1101 72-73 5.211377984911614 1101 74-75 4.7537267655454265 1101 76-77 4.8844088685275615 1101 78-79 4.99258236565402 1101 80-81 4.79875063179238 1101 82-83 4.272408490896666 1101 84-85 3.6208326180087695 1101 86-87 3.5676022852137628 1101 88-89 3.059286789241547 1101 90-91 3.412007502200339 1101 92-93 3.1808459312708983 1101 94-95 2.873204130712743 1101 96-97 3.2196160465454966 1101 98-99 3.082625900231676 1101 100 2.401026631925319 1102 1 5.607006335111283 1102 2 9.125390209305706 1102 3 6.7778195801935475 1102 4 11.800978926910407 1102 5 9.718494832747563 1102 6 8.475165062422253 1102 7 6.376044232343553 1102 8 7.278831404925025 1102 9 5.585592393010977 1102 10-11 6.690712840662904 1102 12-13 8.471360795000631 1102 14-15 5.216897696739583 1102 16-17 6.457800519525435 1102 18-19 6.106180479512435 1102 20-21 6.206644754711007 1102 22-23 5.564136998836151 1102 24-25 6.195776642597654 1102 26-27 7.427832026431076 1102 28-29 6.4085342747219585 1102 30-31 3.1056813999178985 1102 32-33 3.324639884645535 1102 34-35 4.212789955688557 1102 36-37 4.342340733266582 1102 38-39 4.1182478976692565 1102 40-41 5.337388283882362 1102 42-43 5.9952352684460735 1102 44-45 4.960599938693196 1102 46-47 4.174637734844374 1102 48-49 3.009719677786226 1102 50-51 4.141550927314682 1102 52-53 5.421693889099533 1102 54-55 4.540309880914425 1102 56-57 5.6006445710572414 1102 58-59 5.2670094622660315 1102 60-61 5.1928714844880055 1102 62-63 3.958026109983919 1102 64-65 6.190053750526097 1102 66-67 3.4119600269245716 1102 68-69 5.164075587198294 1102 70-71 4.391189373753434 1102 72-73 4.316818849275883 1102 74-75 3.551044921053819 1102 76-77 1.9335760175403642 1102 78-79 3.6156127856167224 1102 80-81 3.6959465929474398 1102 82-83 3.2988410589074704 1102 84-85 2.303673958287888 1102 86-87 2.242243741255951 1102 88-89 2.577165752518926 1102 90-91 2.36354779418053 1102 92-93 2.4400587423841564 1102 94-95 2.6164673630749533 1102 96-97 3.152409152211458 1102 98-99 2.1863303185222414 1102 100 0.8769414817548906 1103 1 6.207923051841362 1103 2 10.514216055377396 1103 3 7.722905578013009 1103 4 11.867641143495863 1103 5 9.929829189599577 1103 6 8.63743727389011 1103 7 7.403412231809543 1103 8 7.816245462731583 1103 9 6.892403242308973 1103 10-11 6.532812833987784 1103 12-13 8.875728549712154 1103 14-15 8.01252771698746 1103 16-17 7.293915153617505 1103 18-19 6.2667282845098775 1103 20-21 7.84426351625341 1103 22-23 6.681652518494385 1103 24-25 4.844320353520377 1103 26-27 5.9828350302358935 1103 28-29 6.791764143667066 1103 30-31 6.120967428887926 1103 32-33 7.306928561413887 1103 34-35 6.214403110128622 1103 36-37 3.7099063163384756 1103 38-39 5.3893690956311175 1103 40-41 5.114250086610262 1103 42-43 6.285903448140353 1103 44-45 4.704508890031558 1103 46-47 5.428792998177929 1103 48-49 4.610725396140584 1103 50-51 5.1451999939101505 1103 52-53 4.694216862643799 1103 54-55 3.7948545263542037 1103 56-57 4.713309502192235 1103 58-59 4.31374644021588 1103 60-61 3.4115818509521922 1103 62-63 1.4888762979998518 1103 64-65 2.862583399192186 1103 66-67 3.1233388845887298 1103 68-69 3.6542965337305695 1103 70-71 1.9761747997374606 1103 72-73 2.7774690061412457 1103 74-75 1.7897695277778887 1103 76-77 2.2641725509270962 1103 78-79 0.9583357903115548 1103 80-81 2.812827976032679 1103 82-83 2.4596447435747137 1103 84-85 2.4151151170890373 1103 86-87 2.0420012118297883 1103 88-89 1.8590227714095242 1103 90-91 1.5624219236333943 1103 92-93 1.012361436908332 1103 94-95 1.6263799189791008 1103 96-97 2.452222248800471 1103 98-99 1.7696024632388863 1103 100 1.7145802686627505 1104 1 6.412312922108008 1104 2 9.371940216419095 1104 3 7.015691254684938 1104 4 11.697408006438081 1104 5 9.134841396981454 1104 6 8.234234631350976 1104 7 4.039225339186633 1104 8 6.798820023505193 1104 9 6.671593246617462 1104 10-11 7.513304959029668 1104 12-13 7.159047521419751 1104 14-15 7.3284356110944024 1104 16-17 7.492656117761314 1104 18-19 7.014346648241986 1104 20-21 7.362107836230425 1104 22-23 6.373320381963193 1104 24-25 6.645627261462362 1104 26-27 8.69824744825495 1104 28-29 7.730308353538291 1104 30-31 6.253106353680881 1104 32-33 8.167931960331977 1104 34-35 7.7319961016552625 1104 36-37 6.801545456555814 1104 38-39 7.219506967238182 1104 40-41 6.878468096088934 1104 42-43 7.228732209689493 1104 44-45 6.55396314825359 1104 46-47 6.305701897598677 1104 48-49 4.590990128774987 1104 50-51 5.397988542907225 1104 52-53 5.965974725633892 1104 54-55 4.966152817320744 1104 56-57 6.179140800961921 1104 58-59 4.9953491976480215 1104 60-61 4.123978327566679 1104 62-63 4.554341375313271 1104 64-65 3.4730243011022814 1104 66-67 3.2074501393160944 1104 68-69 4.271523303800944 1104 70-71 2.0691663263782765 1104 72-73 1.831863663616474 1104 74-75 2.961558507623746 1104 76-77 3.290490230088377 1104 78-79 4.241164073139839 1104 80-81 3.008900071393871 1104 82-83 2.2961735386343136 1104 84-85 1.804207462342518 1104 86-87 0.32490130278675267 1104 88-89 0.5508462191571404 1104 90-91 2.4101049146813125 1104 92-93 2.3500032579624737 1104 94-95 1.797965442460356 1104 96-97 2.5852587752019254 1104 98-99 2.7824321822297406 1104 100 0.9100898670159516 1105 1 5.466390281063418 1105 2 -2.7371999658918202 1105 3 0.5419785175575704 1105 4 8.514456540559785 1105 5 8.144671404372438 1105 6 7.987154069636269 1105 7 5.093598337462069 1105 8 5.520672696577378 1105 9 4.478023401828107 1105 10-11 4.860139154644354 1105 12-13 4.8995870631788065 1105 14-15 5.238906170345448 1105 16-17 5.36112125695816 1105 18-19 6.254972418136045 1105 20-21 7.024450777841661 1105 22-23 2.860152113916879 1105 24-25 4.156122457323409 1105 26-27 7.103063905510428 1105 28-29 5.532661149793526 1105 30-31 5.122273636262797 1105 32-33 6.827330827047916 1105 34-35 5.836504601285714 1105 36-37 5.706854646011344 1105 38-39 4.1295956590341945 1105 40-41 5.079687608284058 1105 42-43 5.44559596923618 1105 44-45 6.103815328593573 1105 46-47 5.28442818486155 1105 48-49 4.8304456596939325 1105 50-51 5.866330499054062 1105 52-53 5.763203107008614 1105 54-55 5.576093689456737 1105 56-57 5.344982141193508 1105 58-59 2.6204046300205235 1105 60-61 4.259171724955198 1105 62-63 -0.6003272622821996 1105 64-65 -0.736214427648644 1105 66-67 0.12856371408820522 1105 68-69 2.157960849994584 1105 70-71 2.4459586397559576 1105 72-73 2.2133492511996202 1105 74-75 2.443647692151952 1105 76-77 1.6458856476789165 1105 78-79 3.655896765921309 1105 80-81 2.6503265311130164 1105 82-83 0.9999059850496863 1105 84-85 1.4502902413521284 1105 86-87 -0.3846084286741984 1105 88-89 1.8058844059026455 1105 90-91 1.394522258854753 1105 92-93 1.9401732505714868 1105 94-95 1.5572780810412894 1105 96-97 1.9331030718267073 1105 98-99 1.6730702704288056 1105 100 1.6865372678535948 1106 1 5.845806738779395 1106 2 9.141646626312419 1106 3 5.384330922390028 1106 4 10.870918815462908 1106 5 8.890009904486412 1106 6 7.729278528264032 1106 7 5.0728324382142915 1106 8 6.647068867081142 1106 9 6.1189169509505135 1106 10-11 4.8094439187200315 1106 12-13 7.05121687854238 1106 14-15 6.735874485822993 1106 16-17 6.738360828419296 1106 18-19 6.490816958824443 1106 20-21 7.627117748436607 1106 22-23 6.376530048724266 1106 24-25 4.678620747726871 1106 26-27 6.980155311938514 1106 28-29 5.449745717835469 1106 30-31 4.9906688949817415 1106 32-33 6.331034952874223 1106 34-35 5.540265713663196 1106 36-37 4.909966111705444 1106 38-39 6.48692412951516 1106 40-41 5.0022998717898695 1106 42-43 4.745630161166883 1106 44-45 3.623065385580123 1106 46-47 4.898640630724326 1106 48-49 3.4751117185040528 1106 50-51 4.371060382801499 1106 52-53 4.754632329712056 1106 54-55 3.531644179541079 1106 56-57 3.0299148970631187 1106 58-59 3.625977914725336 1106 60-61 3.941357729058229 1106 62-63 3.5000602462657824 1106 64-65 4.347988900365944 1106 66-67 2.30775222559949 1106 68-69 2.092961989725609 1106 70-71 2.1238250632800018 1106 72-73 1.440189916802538 1106 74-75 1.5455054537202209 1106 76-77 2.1023707172025077 1106 78-79 2.5798311174143613 1106 80-81 2.549540116706819 1106 82-83 0.9462018592233825 1106 84-85 1.8363513309349848 1106 86-87 1.6879240536599731 1106 88-89 2.0071381100284746 1106 90-91 1.169710314473626 1106 92-93 1.253713255130414 1106 94-95 1.5543375750007122 1106 96-97 0.5525012938463121 1106 98-99 0.9642031630661414 1106 100 2.0107223601718083 1107 1 5.73794248967306 1107 2 9.249424739064906 1107 3 5.2256246904248265 1107 4 10.620649639357133 1107 5 9.20613032310909 1107 6 7.316276245650656 1107 7 2.7836511642576163 1107 8 4.989482407715965 1107 9 4.15483581050519 1107 10-11 3.3318824388974733 1107 12-13 5.360101843441814 1107 14-15 5.024474339391194 1107 16-17 5.709688189632097 1107 18-19 4.80087553154506 1107 20-21 4.487404582494914 1107 22-23 4.450811886874053 1107 24-25 3.8331709006188994 1107 26-27 6.482157982165479 1107 28-29 6.158658677049907 1107 30-31 4.207291057865586 1107 32-33 4.248484024755008 1107 34-35 6.205044558574691 1107 36-37 6.1597938389977465 1107 38-39 5.443532941264159 1107 40-41 6.676855192863126 1107 42-43 5.247945075676551 1107 44-45 3.788113406597393 1107 46-47 2.5859118310344194 1107 48-49 3.2280403545348904 1107 50-51 3.9823808531059903 1107 52-53 2.983884235478886 1107 54-55 4.462347482870246 1107 56-57 4.2801625390804325 1107 58-59 2.6530785650548054 1107 60-61 2.5094808179078782 1107 62-63 -0.2839959146369253 1107 64-65 0.5503877978206155 1107 66-67 2.436375335983225 1107 68-69 3.9641989077668676 1107 70-71 3.4609412181531667 1107 72-73 1.7182272999801071 1107 74-75 1.7872578978392646 1107 76-77 1.8044896715071737 1107 78-79 2.5735431635834445 1107 80-81 1.3442233502655405 1107 82-83 1.1105500223656364 1107 84-85 1.4003183407114577 1107 86-87 1.7468628537805628 1107 88-89 2.1119875867501214 1107 90-91 1.4076727590233489 1107 92-93 1.7072857604062683 1107 94-95 2.032674282007907 1107 96-97 1.334339443637429 1107 98-99 1.047432414971908 1107 100 -0.22666943103367032 1108 1 4.4651004123955005 1108 2 -2.025544243565367 1108 3 1.1674006563943422 1108 4 6.6671535386836105 1108 5 7.3842586463987026 1108 6 6.292946752563097 1108 7 4.714681827143121 1108 8 5.370109844795017 1108 9 5.894932407456626 1108 10-11 3.4501250833497963 1108 12-13 2.8946972883195166 1108 14-15 3.8500459452047373 1108 16-17 4.483283545888742 1108 18-19 4.3005985907439275 1108 20-21 5.336903873526463 1108 22-23 4.159038136430947 1108 24-25 3.1373959094810075 1108 26-27 5.002418971176471 1108 28-29 6.1652831010130384 1108 30-31 4.865601497426024 1108 32-33 4.044146024046043 1108 34-35 3.102698316107478 1108 36-37 0.746864026874384 1108 38-39 1.6423653588465754 1108 40-41 5.501064249935087 1108 42-43 3.4186846183919 1108 44-45 3.557532495572932 1108 46-47 4.750680530077311 1108 48-49 5.29111639140125 1108 50-51 1.6700359399546265 1108 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2212 84-85 -5.047812739677685 2212 86-87 -4.326613848728396 2212 88-89 -1.230971962661041 2212 90-91 -2.1065617519853532 2212 92-93 -4.071208863249648 2212 94-95 -1.6681961737012578 2212 96-97 -4.627872537929388 2212 98-99 -3.026658726861168 2212 100 -0.9828394259133404 2213 1 -20.289707279912193 2213 2 -16.395736551257674 2213 3 -19.428753189759504 2213 4 -18.23188492285485 2213 5 -20.577279815139757 2213 6 -21.144553247436903 2213 7 -9.961983936670531 2213 8 -9.735253102117227 2213 9 -3.606895869899823 2213 10-11 -16.97125899032301 2213 12-13 -15.601409158051013 2213 14-15 -22.72206943941065 2213 16-17 -17.00933563070931 2213 18-19 -17.78583829983029 2213 20-21 -16.72058529223735 2213 22-23 -18.534454550459625 2213 24-25 -7.130514437214551 2213 26-27 -22.374984874977375 2213 28-29 -23.27221689898696 2213 30-31 -18.387276346560974 2213 32-33 -22.306815514415497 2213 34-35 -17.41866544337248 2213 36-37 -22.685828280817923 2213 38-39 -10.428700459138255 2213 40-41 -16.70956007292515 2213 42-43 -21.920257689300406 2213 44-45 -16.03235780778568 2213 46-47 -15.26902423698661 2213 48-49 -5.628777974795934 2213 50-51 -21.743425598506914 2213 52-53 -16.716803763482627 2213 54-55 -16.20147415664426 2213 56-57 -15.66930848058595 2213 58-59 -15.920783964964016 2213 60-61 -17.377551050723262 2213 62-63 -15.800189852457397 2213 64-65 -15.842810099239161 2213 66-67 -8.296960160868853 2213 68-69 -20.554234271956634 2213 70-71 -14.705054757701962 2213 72-73 -7.781772700019893 2213 74-75 -4.028767285518953 2213 76-77 -2.3598512125065874 2213 78-79 -5.006452942082813 2213 80-81 -2.2829266464891838 2213 82-83 -2.2363702086981654 2213 84-85 -1.900551393824788 2213 86-87 -2.889211657805081 2213 88-89 -2.5355103038190983 2213 90-91 -2.26556018704013 2213 92-93 -3.191396656676879 2213 94-95 -3.5948002425588506 2213 96-97 -2.89688662243643 2213 98-99 -2.285500667393247 2213 100 -3.381274480686425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 34.0 4 155.0 5 532.0 6 772.0 7 763.0 8 752.0 9 794.0 10 1072.0 11 1424.0 12 1783.0 13 2259.0 14 2297.0 15 2502.0 16 2463.0 17 2429.0 18 2359.0 19 2385.0 20 2411.0 21 2339.0 22 2571.0 23 2749.0 24 2966.0 25 3128.0 26 3163.0 27 3424.0 28 3561.0 29 3737.0 30 4041.0 31 4424.0 32 5100.0 33 5602.0 34 6129.0 35 5948.0 36 4858.0 37 2601.0 38 423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.94098465327344 18.742161311654847 11.082480103983764 24.23437393108795 2 12.626735728454927 24.720354860039674 33.97481816178091 28.678091249724485 3 22.07875396129728 32.92989908524937 23.957701211426517 21.033645742026835 4 14.050972040942503 22.99512262141925 27.724118980559183 35.22978635707907 5 11.409460219454116 42.40825166624786 32.49452561295186 13.68776250134616 6 32.03116834856526 30.086049725360866 19.552216273965602 18.330565652108277 7 23.458132137217657 34.38908849577774 21.811566011396664 20.341213355607938 8 28.618394490650484 33.548662460058374 21.17338993413236 16.659553115158786 9 28.953137693497588 18.90406080637443 17.13573487352408 35.0070666266039 10-11 22.04951553511734 32.09063707273478 22.19884271436086 23.661004677787016 12-13 28.10762730709362 28.43708671277928 21.929824561403507 21.525461418723594 14-15 28.719841432574672 27.39730708769052 20.64736974004967 23.23548173968514 16-17 21.921542629190622 30.737709602569524 28.21619211997211 19.124555648267744 18-19 25.538819910894002 29.691069045842095 27.082909288282785 17.687201754981118 20-21 22.955949702486322 29.68741077444923 26.14636987631197 21.210269646752476 22-23 20.144158877196126 31.378918304369012 20.74230060542248 27.734622213012383 24-25 22.06463846518502 32.84013273472575 18.973815565656285 26.12141323443295 26-27 25.553363249579487 26.413018805329045 21.386623829395912 26.646994115695556 28-29 24.833568009011547 25.92058575049282 25.65023936919178 23.595606871303858 30-31 27.489889965202668 28.83144041026549 23.077986955150724 20.600682669381115 32-33 19.631707120021236 29.537296723335988 25.968629895086735 24.86236626155604 34-35 17.080979391082124 31.11226958753832 26.861290706437668 24.94546031494189 36-37 25.683585465856073 28.56835854658561 23.579245016259012 22.168810971299308 38-39 28.204265059835386 25.569600368045155 21.86502392854538 24.361110643574076 40-41 22.11983950886955 30.202453352954024 22.73662669717071 24.941080441005713 42-43 27.81096770557403 27.128445020571494 22.391365608972553 22.669221664881924 44-45 29.271106508942342 25.72230913818137 22.084032100127185 22.92255225274911 46-47 23.17627858196441 27.710133873238252 29.103268032506463 20.010319512290874 48-49 26.089278439058766 27.15075371351179 27.366021780063203 19.39394606736624 50-51 22.339117982025865 27.988453254851546 26.926806716056067 22.74562204706652 52-53 20.177414267294573 29.4161266580708 21.30520677118208 29.10125230345255 54-55 22.107677866216388 31.079722501485808 19.52654176641468 27.286057865883123 56-57 25.695102291801696 25.08572789771374 21.391257139375004 27.827912671109562 58-59 24.58975906920586 24.25278524779101 25.918445749410026 25.23900993359311 60-61 27.380007058408328 26.847317804835008 23.319767954826187 22.452907181930478 62-63 20.233297057263325 28.156975122636812 25.418222982986705 26.191504837113154 64-65 17.755322388638188 29.54403255723817 26.610558954624736 26.090086099498905 66-67 25.008015738018507 27.137213131680916 23.55594441697054 24.298826713330037 68-69 28.21454608188501 24.291144242059982 21.841939774390482 25.652369901664525 70-71 22.68044599803204 28.301630360919177 22.469515675710937 26.548407965337844 72-73 27.62029505775564 25.86202249855813 22.469451227071577 24.048231216614653 74-75 28.908589334976032 24.50277204917374 21.692690896429813 24.895947719420413 76-77 23.296382229096846 26.488785769528228 27.857802698289934 22.35702930308499 78-79 26.077265171304592 26.259687830573608 26.45506357807474 21.207983420047064 80-81 22.697191915578607 27.13777011755906 26.118816496208737 24.046221470653595 82-83 20.529925420679835 28.423126549274286 21.66387373448049 29.383074295565393 84-85 22.46033880075289 29.296047324549612 20.170475934390968 28.073137940306538 86-87 25.142597735526596 24.33614612262337 21.97714163640248 28.544114505447553 88-89 24.70289079229122 23.582976445396145 25.684154175588862 26.029978586723768 90-91 26.796507006790748 25.956332250441427 23.394732771083053 23.85242797168478 92-93 21.08576428159877 27.32282708435586 24.774332600670068 26.817076033375304 94-95 19.048688057555495 28.40840455612035 26.019701853128723 26.523205533195434 96-97 25.000802027525587 26.33965331023494 23.558756536524335 25.10078812571514 98-99 27.24018167245525 24.16671119422923 22.205183008282127 26.387924125033397 100 22.243924305978034 26.800294579104094 25.449073036406133 25.506708078511735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 265.0 1 297.0 2 269.0 3 160.5 4 94.5 5 68.5 6 51.0 7 43.0 8 49.5 9 60.0 10 52.5 11 48.5 12 71.0 13 93.0 14 115.0 15 144.0 16 177.5 17 222.5 18 252.0 19 258.0 20 266.0 21 280.0 22 289.0 23 324.0 24 378.0 25 424.5 26 496.5 27 567.0 28 618.5 29 704.0 30 780.5 31 853.0 32 928.0 33 976.5 34 1047.5 35 1117.5 36 1163.5 37 1234.5 38 1303.0 39 1314.0 40 1349.5 41 1389.5 42 1421.0 43 1506.5 44 1627.5 45 1861.0 46 2377.5 47 3263.0 48 4636.0 49 7094.0 50 10344.0 51 10467.5 52 7227.5 53 4523.0 54 3122.0 55 2364.0 56 1875.5 57 1611.5 58 1392.5 59 1176.0 60 970.5 61 829.5 62 757.5 63 634.5 64 528.0 65 442.5 66 353.5 67 283.0 68 206.0 69 157.0 70 117.0 71 88.0 72 68.0 73 50.0 74 37.0 75 22.5 76 15.5 77 14.5 78 14.0 79 11.5 80 5.0 81 2.0 82 3.0 83 4.5 84 3.5 85 1.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 6.670923117850493 2 22.75392391593509 3 5.308858739026337 4 22.54855014631551 5 11.071029529130087 6 8.366054801808993 7 7.0029263101888795 8 7.753125831338122 9 4.379888268156424 10-11 5.936153232242618 12-13 9.079010375099761 14-15 6.585794094173983 16-17 6.903963820164938 18-19 5.896249002394254 20-21 6.827347698856078 22-23 4.999733971801011 24-25 4.599627560521415 26-27 8.585794094173982 28-29 5.533386538973131 30-31 3.8249534450651765 32-33 7.802075019952115 34-35 6.104283054003725 36-37 5.919127427507316 38-39 5.166799680766161 40-41 7.440276669326949 42-43 5.597233306730513 44-45 5.456770417664273 46-47 5.132748071295557 48-49 3.3604682096302207 50-51 5.631284916201118 52-53 3.853684490555999 54-55 4.210162277201383 56-57 3.956903431763767 58-59 3.0540037243947857 60-61 3.5147645650438943 62-63 2.7097632349028995 64-65 3.9079542431497742 66-67 2.094706038840117 68-69 4.3942537909018355 70-71 2.1298217611066774 72-73 1.2913008778930566 74-75 0.6730513434424049 76-77 0.9353551476456504 78-79 1.4184623570098431 80-81 1.8339984038308061 82-83 0.6219739292364991 84-85 1.0641127959563714 86-87 0.37776004256451184 88-89 0.6118648576749135 90-91 0.2601755786113328 92-93 0.26922053737696194 94-95 0.45810055865921784 96-97 0.49162011173184356 98-99 0.4256451183825486 100 0.30007980845969673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 93975.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.11013567438148 #Duplication Level Percentage of deduplicated Percentage of total 1 94.04425826427466 65.93455706304869 2 2.9672464559997573 4.160681032189412 3 1.0093191269768995 2.1229050279329607 4 0.503900676926813 1.4131417930300612 5 0.3263212215038096 1.1439212556530993 6 0.21855932975138875 0.9193934557063049 7 0.14874176608080625 0.7299813780260708 8 0.08803084114986492 0.49374833732375634 9 0.08044197553349726 0.5075818036711891 >10 0.5266672737759159 6.775206171854216 >50 0.03339100871201773 1.602553870710295 >100 0.042497647451658925 6.520883213620644 >500 0.007588865616367666 3.39877627028465 >1k 0.0030355462465470663 4.276669326948657 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2302 2.449587656291567 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1717 1.8270816706570898 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 766 0.8151104017025805 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 736 0.7831870178238893 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 681 0.7246608140462889 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 508 0.5405693003458367 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 503 0.5352487363660549 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 478 0.5086459164671455 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 448 0.47672253258845443 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 426 0.45331205107741424 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 306 0.32561851556264965 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 279 0.29688747007182764 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 270 0.28731045490822027 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 259 0.27560521415270023 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 233 0.24793828145783453 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 229 0.24368183027400903 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 227 0.2415536046820963 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 223 0.2372971534982708 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 215 0.22878425113061984 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 207 0.22027134876296886 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 206 0.2192072359670125 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 198 0.21069433359936154 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 197 0.20963022080340518 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 195 0.20750199521149243 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 171 0.1819632881085395 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 168 0.1787709497206704 No Hit CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 168 0.1787709497206704 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 164 0.17451449853684492 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 161 0.1713221601489758 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 134 0.14259111465815377 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 127 0.13514232508645918 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 114 0.12130885873902633 No Hit GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 113 0.12024474594306996 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 107 0.11386006916733174 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 105 0.11173184357541899 No Hit NNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGNN 100 0.10641127959563715 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 95 0.10109071561585528 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0010641127959563714 0.0 20-21 0.0 0.0 0.0 0.0015961691939345571 0.0 22-23 0.0 0.0 0.0 0.002128225591912743 0.0 24-25 0.0 0.0 0.0 0.005320563979781857 0.0 26-27 0.0 0.0 0.0 0.005320563979781857 0.0 28-29 0.0 0.0 0.0 0.006384676775738229 0.0 30-31 0.0 0.0 0.0 0.010641127959563713 0.0 32-33 0.0 0.0 0.0 0.03139132748071296 0.0 34-35 0.0 0.0 0.0 0.06757116254322959 0.0 36-37 0.0 0.0 0.0 0.11758446395317904 0.0 38-39 0.0 0.0 0.0 0.21122638999733973 0.0 40-41 0.0 0.0 0.0 0.401170524075552 0.0 42-43 0.0 0.0 0.0 0.48842777334397447 0.0 44-45 0.0 0.0 0.0 0.578877361000266 0.0 46-47 0.0 0.0 0.0 0.6863527533918595 0.0 48-49 0.0 0.0 0.0 0.7847831870178239 0.0 50-51 0.0 0.0 0.0 0.8933226922053737 0.0 52-53 0.0 0.0 0.0 1.077414205905826 0.0 54-55 0.0 0.0 0.0 1.2109603617983506 0.0 56-57 0.0 0.0 0.0 1.3615323224261773 0.0 58-59 0.0 0.0 0.0 1.5073157754722 0.0 60-61 0.0 0.0 0.0 1.6786379356211758 0.0 62-63 0.0 0.0 0.0 1.87975525405693 0.0 64-65 0.0 0.0 0.0 2.1149241819632882 0.0 66-67 0.0 0.0 0.0 2.3351955307262573 0.0 68-69 0.0 0.0 0.0 2.647512636339452 0.0 70-71 0.0 0.0 0.0 3.2764032987496674 0.0 72-73 0.0 0.0 0.0 3.6914072891726524 0.0 74-75 0.0 0.0 0.0 4.016493748337324 0.0 76-77 0.0 0.0 0.0 4.407023144453312 0.0 78-79 0.0 0.0 0.0 4.752327746741155 5.320563979781857E-4 80-81 0.0 0.0 0.0 5.171056131949987 0.0010641127959563714 82-83 0.0 0.0 0.0 5.878159084862995 0.0010641127959563714 84-85 0.0 0.0 0.0 6.46608140462889 0.0010641127959563714 86-87 0.0 0.0 0.0 6.927374301675978 0.0010641127959563714 88 0.0 0.0 0.0 7.3774940143655225 0.0010641127959563714 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 30 0.003795314 61.1106 1 ATACTAC 25 0.0041524908 59.750904 7 GATACTA 30 0.008416696 49.97879 6 TGTACTG 30 0.008584084 49.730602 5 AATTGTG 15 0.009646371 48.3063 36-37 AAAAGTA 70 0.0024298914 34.920345 2 ACAGCAG 30 0.0059381425 29.122501 82-83 GACTCTG 120 1.2285815E-4 24.896208 7 CATGGAC 115 0.001490701 24.139324 3 AGTACTC 110 0.0021866478 22.604818 5 AAAGTAC 125 0.0024210957 22.208178 3 ATGGACT 135 0.003768406 20.577389 4 GGGACGC 65 0.006839142 18.579348 36-37 TGGGACG 65 0.006839142 18.579348 36-37 GGACTCT 135 0.0069561577 18.510664 6 ATGTACC 190 1.7357377E-5 18.313274 94 TGGACTC 140 0.008829121 17.76093 5 CATGGGC 300 8.4511715E-4 11.598407 94 TGCCGAC 355 2.7699588E-4 11.026655 94 TACATGG 5835 0.0 10.892035 2 >>END_MODULE