FastQCFastQC Report
Wed 25 May 2016
SRR1295069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350417
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25560.7294166664288547No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19550.5579067225619762No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11600.3310341678628605No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9550.27253243992157916No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8090.23086779465608118No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6510.18577865799889845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6370.1817834180419329No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6040.1723660667147998No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5000.14268714132019852No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4950.1412602699069965No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4880.13926264992851375No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3650.10416161316374492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC707.370383E-540.3670273
TAATACT600.001681271237.683134
CTGTGCG550.001935691136.6186379
TACATAG1301.1758239E-633.657912
GACTGAT600.00305944133.355797
GGATAAT952.451773E-432.90571
GTATAGA800.004201956631.2604181
TTAGGCA800.006879804728.262354
GTACATA1355.866823E-527.7870351
GTCGGGA950.00973699926.3189642
GGACTGA1000.001184908225.1408796
GTACATT2001.1796747E-625.0083331
ATATACT1401.491531E-424.224874
ACATAGG1401.493478E-424.2202173
TAATGAT1050.00157761523.9355095
GAACCTT1301.9962063E-423.2069646
TATTATA1702.8097274E-422.0614852
ATGGGCG1654.0764084E-521.3243625
ATACTGA1250.00433241420.1127036
TGGACTG1505.262644E-420.1058275
Produced by FastQC (version 0.11.5)