##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295069_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 350417 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.62743816652731 27.0 22.0 33.0 6.0 33.0 2 21.70925497336031 27.0 2.0 33.0 2.0 33.0 3 23.986487527716978 27.0 15.0 33.0 6.0 33.0 4 23.82337329524538 33.0 6.0 37.0 2.0 37.0 5 26.030406629815335 33.0 15.0 37.0 2.0 37.0 6 26.727918451444992 33.0 15.0 37.0 2.0 37.0 7 26.447241429496856 33.0 15.0 37.0 6.0 37.0 8 27.245615937582937 33.0 15.0 37.0 6.0 37.0 9 28.071132393690945 33.0 15.0 37.0 6.0 37.0 10-11 27.714571496245902 33.0 15.0 37.0 6.0 37.0 12-13 26.507830670315652 33.0 15.0 37.0 4.0 37.0 14-15 28.135354449127753 33.0 15.0 37.0 6.0 40.0 16-17 28.534417565357842 33.0 22.0 37.0 6.0 40.0 18-19 28.71137530428033 33.0 18.5 37.0 6.0 40.0 20-21 28.17834608480753 33.0 15.0 37.0 6.0 40.0 22-23 29.048632058376164 33.0 22.0 37.0 6.0 40.0 24-25 29.174710416446693 33.0 24.5 37.0 6.0 40.0 26-27 27.6766894870968 33.0 15.0 37.0 4.0 40.0 28-29 28.409651928987465 33.0 22.0 37.0 6.0 40.0 30-31 29.091197059503394 33.0 22.0 37.0 6.0 40.0 32-33 27.7535664650973 33.0 22.0 37.0 6.0 40.0 34-35 27.323745708684225 33.0 15.0 37.0 6.0 40.0 36-37 27.610947813604934 33.0 22.0 37.0 6.0 40.0 38-39 27.714357465533922 33.0 18.5 37.0 6.0 40.0 40-41 26.667995559576163 33.0 15.0 37.0 6.0 40.0 42-43 27.20157269767163 33.0 15.0 37.0 6.0 40.0 44-45 27.026475599071965 33.0 15.0 37.0 6.0 40.0 46-47 26.645256936735375 33.0 15.0 37.0 6.0 40.0 48-49 26.48375792270352 33.0 15.0 37.0 6.0 40.0 50-51 26.40693659268814 33.0 15.0 37.0 6.0 40.0 52-53 26.699224067325503 33.0 18.5 37.0 6.0 40.0 54-55 26.560018492253512 33.0 15.0 37.0 6.0 40.0 56-57 26.438337751878475 27.0 15.0 37.0 6.0 38.5 58-59 26.31247913200558 27.0 15.0 37.0 6.0 37.0 60-61 26.21826566633468 27.0 15.0 37.0 6.0 37.0 62-63 26.025846348778742 27.0 15.0 37.0 6.0 37.0 64-65 25.588510260632333 27.0 15.0 33.0 6.0 37.0 66-67 25.769858482893238 27.0 15.0 33.0 6.0 37.0 68-69 25.297869395605808 27.0 15.0 33.0 6.0 37.0 70-71 25.32455902538975 27.0 15.0 33.0 6.0 37.0 72-73 25.276209487553402 27.0 15.0 33.0 6.0 37.0 74-75 25.350118573014434 27.0 15.0 33.0 6.0 37.0 76-77 25.03716429282826 27.0 15.0 33.0 6.0 37.0 78-79 24.625967062100298 27.0 15.0 33.0 6.0 37.0 80-81 24.47799906967984 27.0 15.0 33.0 6.0 37.0 82-83 24.586760916279747 27.0 15.0 33.0 6.0 37.0 84-85 24.440288285100323 27.0 15.0 33.0 6.0 37.0 86-87 24.13708239040914 27.0 15.0 33.0 6.0 37.0 88-89 24.036296755009033 27.0 15.0 33.0 6.0 37.0 90-91 24.137029596166855 27.0 15.0 33.0 6.0 37.0 92-93 23.961129168961552 27.0 15.0 33.0 6.0 33.0 94-95 23.78275169298293 27.0 15.0 33.0 6.0 33.0 96-97 23.872942808140017 27.0 15.0 33.0 6.0 33.0 98-99 23.89195872346376 27.0 15.0 33.0 6.0 37.0 100 23.49728180995785 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.1026818736340935 1101 2 11.107269064810072 1101 3 8.174163580068758 1101 4 12.215027232899118 1101 5 10.433942522101475 1101 6 9.541360820125401 1101 7 9.437863012332347 1101 8 9.228016739276747 1101 9 7.021061614699018 1101 10-11 7.374090346027529 1101 12-13 8.952529400772502 1101 14-15 7.750693176180892 1101 16-17 7.912334474843249 1101 18-19 8.105037606276245 1101 20-21 7.387175163218593 1101 22-23 7.167407613972941 1101 24-25 7.302873089843679 1101 26-27 8.039207833031305 1101 28-29 8.010098029178895 1101 30-31 5.249603247992887 1101 32-33 6.809776000071331 1101 34-35 8.785642787667584 1101 36-37 8.050288640568393 1101 38-39 8.197441173462767 1101 40-41 8.822691689293833 1101 42-43 8.637016261535734 1101 44-45 8.407991860510851 1101 46-47 7.264425250455954 1101 48-49 5.1356982813900025 1101 50-51 6.468041900386911 1101 52-53 5.600152002974102 1101 54-55 3.6620133367568073 1101 56-57 5.626048370223785 1101 58-59 5.91743114794679 1101 60-61 6.195338946371201 1101 62-63 6.05513980973544 1101 64-65 6.509323997289886 1101 66-67 5.477974878405043 1101 68-69 6.44357960789916 1101 70-71 5.902245796958539 1101 72-73 5.383152831679386 1101 74-75 4.887952053638919 1101 76-77 4.915609713928809 1101 78-79 4.753447022840074 1101 80-81 4.438307157557254 1101 82-83 4.084462736935315 1101 84-85 3.9459230184457468 1101 86-87 3.6810637154234342 1101 88-89 3.994557905328641 1101 90-91 3.4759654369590507 1101 92-93 3.314892432153428 1101 94-95 3.4408583429615653 1101 96-97 3.5029120456261182 1101 98-99 2.9323213385117732 1101 100 3.1079531382663603 1102 1 6.8421271226036815 1102 2 10.386043989617917 1102 3 7.942599583187839 1102 4 12.41451620471598 1102 5 10.556220287501944 1102 6 9.49664237298271 1102 7 6.52409031544029 1102 8 7.254473239221053 1102 9 6.270595144831578 1102 10-11 7.578543392702372 1102 12-13 9.157643859945946 1102 14-15 6.478626366556295 1102 16-17 7.400101824737426 1102 18-19 7.419239873180562 1102 20-21 6.868095153192968 1102 22-23 6.628530483528465 1102 24-25 7.248919235549373 1102 26-27 8.439961147056039 1102 28-29 7.743016181122744 1102 30-31 4.094157386346456 1102 32-33 4.103690308860461 1102 34-35 5.241136688748121 1102 36-37 5.3892764705784195 1102 38-39 4.809543629739917 1102 40-41 5.852674562191236 1102 42-43 7.1186115884823735 1102 44-45 6.844026309658673 1102 46-47 5.756731978761625 1102 48-49 3.935164974049016 1102 50-51 4.758227931354941 1102 52-53 5.948423975793531 1102 54-55 5.300394755939166 1102 56-57 6.000010996936492 1102 58-59 5.762622330970071 1102 60-61 5.847307170722324 1102 62-63 4.596422393003788 1102 64-65 6.382297986373096 1102 66-67 3.7166368757315453 1102 68-69 6.040842056378608 1102 70-71 5.3342943655226485 1102 72-73 5.0242634476981 1102 74-75 4.254729651945706 1102 76-77 3.223664325714484 1102 78-79 4.332217770305821 1102 80-81 4.3957818150152015 1102 82-83 3.5407607763694244 1102 84-85 3.124761995832401 1102 86-87 3.2339593097199675 1102 88-89 3.5892025527609697 1102 90-91 3.3918616160834816 1102 92-93 3.242164906253908 1102 94-95 3.274913428179346 1102 96-97 3.3924074227008667 1102 98-99 3.1420343551097467 1102 100 2.836822749495056 1103 1 6.6512588285940275 1103 2 10.583212004848757 1103 3 7.869134566529105 1103 4 12.09309783251227 1103 5 10.468527352165026 1103 6 9.103377261131204 1103 7 8.109053258256637 1103 8 7.862846802830219 1103 9 7.024263466039169 1103 10-11 7.42448410120852 1103 12-13 8.622414037412465 1103 14-15 8.387980755622724 1103 16-17 7.886336270919514 1103 18-19 7.23046072039617 1103 20-21 8.198063875570064 1103 22-23 6.605000871778962 1103 24-25 5.11932618462124 1103 26-27 5.5314328952031815 1103 28-29 6.822012577396631 1103 30-31 6.052666939620416 1103 32-33 7.8556536596457995 1103 34-35 7.049923320964076 1103 36-37 3.823964034323577 1103 38-39 6.1801936603375935 1103 40-41 6.4754324325930845 1103 42-43 6.993065929952422 1103 44-45 5.766687287424364 1103 46-47 5.969533705830369 1103 48-49 5.313540917477315 1103 50-51 6.298630460762709 1103 52-53 6.037320419663796 1103 54-55 4.902058929461976 1103 56-57 5.521907032837763 1103 58-59 5.272092311251573 1103 60-61 5.013722823132738 1103 62-63 2.91365908301535 1103 64-65 4.931958012211133 1103 66-67 3.7425939029974735 1103 68-69 4.773401381869871 1103 70-71 3.2339050951072004 1103 72-73 4.071740839416329 1103 74-75 3.1750652064435627 1103 76-77 3.517922162118918 1103 78-79 2.999578550886497 1103 80-81 3.8733928165790843 1103 82-83 3.3655244943268627 1103 84-85 3.0666366133614673 1103 86-87 2.4065420934284063 1103 88-89 2.4250290299487 1103 90-91 2.1910524775780047 1103 92-93 2.2277551013990795 1103 94-95 2.4205039632986782 1103 96-97 2.607602567869833 1103 98-99 2.5152188930848602 1103 100 2.745985053159977 1104 1 6.4393789628575995 1104 2 9.534559034003635 1104 3 7.327850275718426 1104 4 11.87827436099056 1104 5 9.296880866039515 1104 6 8.024462265436778 1104 7 3.9254126769164586 1104 8 6.359527118455052 1104 9 6.338679226192411 1104 10-11 7.1787105919032115 1104 12-13 7.299992548211538 1104 14-15 6.902268227285031 1104 16-17 6.85149715874617 1104 18-19 6.61590173405099 1104 20-21 7.125898963351027 1104 22-23 6.014512672562752 1104 24-25 6.021509725032118 1104 26-27 8.179850377585474 1104 28-29 7.180052575287778 1104 30-31 6.289847756097572 1104 32-33 7.285964891039576 1104 34-35 7.823761255695111 1104 36-37 6.471090723601822 1104 38-39 7.082319207321056 1104 40-41 6.866073523572471 1104 42-43 7.197942649984757 1104 44-45 6.3395779961743415 1104 46-47 6.030427683300797 1104 48-49 5.085427285507123 1104 50-51 5.294143878332957 1104 52-53 5.614581723988703 1104 54-55 4.154243348561145 1104 56-57 5.481292302507203 1104 58-59 3.027327536107908 1104 60-61 3.3798959598742115 1104 62-63 3.065906767671695 1104 64-65 2.203913796616174 1104 66-67 2.7260701696266487 1104 68-69 3.449442188154247 1104 70-71 1.995546836315924 1104 72-73 1.5569788760752061 1104 74-75 2.3838547254495914 1104 76-77 3.059486754495417 1104 78-79 3.858786325520523 1104 80-81 2.59807956420223 1104 82-83 2.6399485141500705 1104 84-85 2.7025503613185187 1104 86-87 0.41782972325459866 1104 88-89 -0.287130258665254 1104 90-91 1.678301831742111 1104 92-93 1.8481389124459433 1104 94-95 1.9501236670049842 1104 96-97 2.6957437906524646 1104 98-99 2.237365352849725 1104 100 0.8074194239312895 1105 1 5.7266278165614395 1105 2 -2.600753635809795 1105 3 0.4424106342933598 1105 4 8.000470774530879 1105 5 8.446650541311836 1105 6 7.565677428155784 1105 7 5.267562989994712 1105 8 6.116482143867131 1105 9 4.791887967521937 1105 10-11 4.738047178551128 1105 12-13 5.14042030935574 1105 14-15 5.647610233756144 1105 16-17 5.9189554889718075 1105 18-19 5.925521565063317 1105 20-21 7.079566755891847 1105 22-23 1.616114823444093 1105 24-25 4.109937017358057 1105 26-27 6.7031137078288765 1105 28-29 5.916320107137228 1105 30-31 5.378473941017965 1105 32-33 7.2086720323492095 1105 34-35 5.793951340188947 1105 36-37 6.004232977978344 1105 38-39 4.163699131244638 1105 40-41 5.515393914680551 1105 42-43 5.609280948529644 1105 44-45 6.023182498250282 1105 46-47 5.127310574630858 1105 48-49 4.8313375565338 1105 50-51 6.008179835272713 1105 52-53 5.680947920771615 1105 54-55 5.090735344910044 1105 56-57 4.7430731370747345 1105 58-59 2.49368449859179 1105 60-61 4.676423508274368 1105 62-63 -0.3995409715232867 1105 64-65 0.1418175752203048 1105 66-67 1.1475262410006764 1105 68-69 2.626769142717947 1105 70-71 2.609797313125405 1105 72-73 2.367851552849878 1105 74-75 2.210915838750889 1105 76-77 1.5668058243884069 1105 78-79 2.8666150576867793 1105 80-81 1.1767264907789006 1105 82-83 0.9097161294633764 1105 84-85 1.6561515603567294 1105 86-87 -0.31214250281524514 1105 88-89 1.7722356291238057 1105 90-91 1.1994394973823503 1105 92-93 1.515610028624085 1105 94-95 1.2411843327979462 1105 96-97 2.1541308427445642 1105 98-99 2.0688269648031685 1105 100 2.099265893054991 1106 1 6.304225469795963 1106 2 10.21999156515773 1106 3 6.917203491068616 1106 4 11.694583275174182 1106 5 9.78856809573518 1106 6 9.63415563308605 1106 7 6.671458826544555 1106 8 7.161106712854831 1106 9 6.978291137713899 1106 10-11 5.700084644456123 1106 12-13 8.266548660958847 1106 14-15 7.560478845767879 1106 16-17 8.010973398497825 1106 18-19 7.341601540360518 1106 20-21 8.165061583744247 1106 22-23 6.192230309008394 1106 24-25 4.744608418727005 1106 26-27 7.585044642932573 1106 28-29 5.880560238885469 1106 30-31 5.2813147623858825 1106 32-33 7.187679803436648 1106 34-35 6.810911352540341 1106 36-37 6.2568602231021195 1106 38-39 7.148519778663715 1106 40-41 6.5696731591867525 1106 42-43 5.783389332040528 1106 44-45 3.470158979131348 1106 46-47 5.728378455851587 1106 48-49 5.745045034275552 1106 50-51 6.351062342606355 1106 52-53 5.594055841105099 1106 54-55 5.253179377641246 1106 56-57 4.722093774200978 1106 58-59 4.858328623958474 1106 60-61 5.0216296222160075 1106 62-63 5.093758395467603 1106 64-65 5.0795176421237045 1106 66-67 2.9997494925060053 1106 68-69 3.1810535336396697 1106 70-71 2.774207276585855 1106 72-73 2.8368032280081295 1106 74-75 2.736354570674102 1106 76-77 3.8628971217997616 1106 78-79 3.926197683387983 1106 80-81 3.579389760811324 1106 82-83 2.0491634044363565 1106 84-85 2.5529196114091732 1106 86-87 3.261494983675412 1106 88-89 3.3692867330299343 1106 90-91 3.4981528935019526 1106 92-93 2.720030799557126 1106 94-95 1.413607265225508 1106 96-97 1.6290523599078632 1106 98-99 2.3598670553800822 1106 100 2.9956582557743587 1107 1 5.97252828761183 1107 2 9.930773714662124 1107 3 5.911940105361133 1107 4 10.98105689331901 1107 5 9.343353188580267 1107 6 7.704882378530364 1107 7 3.3073589260987255 1107 8 6.189345469726234 1107 9 5.696440295831891 1107 10-11 4.586077805517455 1107 12-13 6.342002065278631 1107 14-15 5.653388857454669 1107 16-17 6.656590850776745 1107 18-19 5.968299371810982 1107 20-21 5.128209226907472 1107 22-23 5.681413531966456 1107 24-25 4.589554962465911 1107 26-27 6.9798288700892215 1107 28-29 6.194021694107732 1107 30-31 4.682795089616107 1107 32-33 4.721216573553086 1107 34-35 6.371734622245576 1107 36-37 6.338529270411993 1107 38-39 6.026959808097121 1107 40-41 6.945342985616222 1107 42-43 5.089479450207001 1107 44-45 3.6282336880436112 1107 46-47 2.3033730452988443 1107 48-49 3.4828150208573803 1107 50-51 3.9626452539165733 1107 52-53 3.208456568283392 1107 54-55 4.65281578849698 1107 56-57 4.810554147312697 1107 58-59 2.6103867982215547 1107 60-61 2.5291481263063886 1107 62-63 -0.05544300694766591 1107 64-65 1.6076525163824584 1107 66-67 3.4050734764041977 1107 68-69 4.5152049425476015 1107 70-71 3.7060819248999266 1107 72-73 1.9165331332152462 1107 74-75 1.6467991016873924 1107 76-77 2.269865913731543 1107 78-79 3.2023479669009447 1107 80-81 1.5161252497088675 1107 82-83 1.0356586647921553 1107 84-85 1.942821355771379 1107 86-87 1.7861671041792455 1107 88-89 2.50128257772543 1107 90-91 1.0457484438633777 1107 92-93 1.3850699719589805 1107 94-95 1.7379504241226194 1107 96-97 1.8992943198264882 1107 98-99 1.1270964195319237 1107 100 -0.3692467349194928 1108 1 4.425508420062162 1108 2 -3.071875291960392 1108 3 0.16227123118894937 1108 4 6.098937688021 1108 5 6.62414789056702 1108 6 6.396273107007186 1108 7 3.72060395921131 1108 8 4.883385204825572 1108 9 4.431539633580236 1108 10-11 3.1595877392922915 1108 12-13 2.5221345156097605 1108 14-15 3.666633890567251 1108 16-17 4.104272969982045 1108 18-19 3.7941271863805177 1108 20-21 4.474566842801515 1108 22-23 4.24167843262871 1108 24-25 2.630614565114918 1108 26-27 4.486451386645513 1108 28-29 5.864220369604421 1108 30-31 4.390742109483661 1108 32-33 4.194064255672291 1108 34-35 2.8147809798614674 1108 36-37 1.1829001313391423 1108 38-39 2.042853847832216 1108 40-41 5.251303250516884 1108 42-43 2.860340377359318 1108 44-45 3.467968787381359 1108 46-47 4.447743906225998 1108 48-49 4.392086544036189 1108 50-51 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48-49 -5.493431057915711 2213 50-51 -22.223447461315214 2213 52-53 -16.921394549577485 2213 54-55 -16.512978664522986 2213 56-57 -16.334108004756217 2213 58-59 -16.81717631485916 2213 60-61 -17.78661894036692 2213 62-63 -16.15770113571329 2213 64-65 -16.344289424562618 2213 66-67 -7.75296317150697 2213 68-69 -21.19737783890935 2213 70-71 -16.012027919246865 2213 72-73 -8.40912040460281 2213 74-75 -3.4845383478968337 2213 76-77 -2.3215173457416434 2213 78-79 -3.887810375976116 2213 80-81 -3.100906568246618 2213 82-83 -2.602657323854956 2213 84-85 -2.87413577214776 2213 86-87 -2.91929522714576 2213 88-89 -2.234745406141524 2213 90-91 -2.184408903146533 2213 92-93 -3.077644761895524 2213 94-95 -3.3235493518072694 2213 96-97 -2.3655689974069958 2213 98-99 -3.1182055771817154 2213 100 -3.1615337438325035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94.0 3 104.0 4 611.0 5 1789.0 6 2803.0 7 3337.0 8 3334.0 9 3551.0 10 4117.0 11 4929.0 12 6396.0 13 8025.0 14 7756.0 15 8783.0 16 9477.0 17 9284.0 18 9173.0 19 8865.0 20 8817.0 21 8982.0 22 9224.0 23 9619.0 24 10534.0 25 11015.0 26 11847.0 27 12388.0 28 12741.0 29 13320.0 30 14355.0 31 15697.0 32 17738.0 33 20124.0 34 21970.0 35 23061.0 36 20946.0 37 12998.0 38 2610.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37489740865887 18.827253215561054 11.166273851518971 25.63157552426111 2 16.376138903214716 22.721143009951675 34.212366053521286 26.69035203331232 3 20.822544581660445 27.755472562554452 26.654562956554585 24.76741989923052 4 15.079701800109097 18.940163040184256 30.501848596884663 35.478286562821985 5 13.885188543074753 38.714745341655565 32.438704271635565 14.961361843634114 6 32.591037837700526 33.85578742807789 17.255591825329287 16.297582908892306 7 26.596659603639537 32.78729901533092 20.729153683160913 19.886887697868627 8 27.993576137793724 32.85561336614136 20.33847367531321 18.812336820751703 9 26.94362483805014 17.598400584680522 18.665382141271618 36.79259243599772 10-11 24.644913686475682 28.411349887423803 24.193382757526667 22.750353668573847 12-13 26.157388987736212 26.159139593286586 24.16408584969619 23.51938556928101 14-15 25.182073997839684 26.928562708710963 22.034623806455304 25.85473948699405 16-17 23.30036066123295 29.545200598412098 25.05214379271601 22.102294947638942 18-19 24.317211067764287 28.304524093170354 25.92785736023453 21.45040747883083 20-21 23.94590888100047 27.848498842392832 25.079881891416605 23.125710385190093 22-23 22.976390338339613 28.082943872226508 23.737011661146152 25.203654128287734 24-25 23.55617712241699 28.700698960987626 22.68296620154758 25.060157715047804 26-27 24.339173539050567 26.755389618210284 23.820971494133385 25.084465348605768 28-29 24.021156281285286 26.507369803690313 25.379309083761136 24.092164831263265 30-31 24.992289641300705 27.838343204082395 24.161060826163073 23.00830632845383 32-33 22.398218042321496 27.724510846664863 25.375979598965536 24.501291512048105 34-35 21.746133425313978 28.01364927041693 25.625026947454003 24.615190356815088 36-37 24.415406094060295 27.44431287081876 24.37624118213527 23.764039852985672 38-39 25.083875161561252 26.16662034358061 24.14906009698991 24.600444397868227 40-41 23.388210418983185 27.802148479346418 24.41131071627968 24.398330385390715 42-43 25.008201686724558 26.961042369532144 24.506487343462226 23.52426860028107 44-45 25.35075829731116 26.382274647715338 24.181258700271083 24.08570835470242 46-47 23.271559427043986 27.12651211053767 26.41335117373456 23.188577288683785 48-49 24.091739274520847 26.95127261270874 26.084733848808227 22.872254263962184 50-51 22.846862503327348 27.213871134541073 25.872376750490307 24.066889611641276 52-53 21.971159237192474 27.45613707211686 24.291876294208674 26.280827396481993 54-55 22.940927026441788 27.85349950677285 23.679715131246542 25.52585833553882 56-57 23.819350229916797 26.020968652075254 23.986835312338666 26.172845805669283 58-59 23.635900730341902 25.522888684289214 25.350442734622707 25.49076785074618 60-61 24.370569433780336 26.481319453124534 24.584397264432262 24.563713848662868 62-63 22.21422102449692 26.81967116549962 25.225272985649383 25.740834824354074 64-65 21.659653070427094 26.965017909763557 25.5794269175282 25.795902102281154 66-67 23.716571777115366 26.265173653010688 24.383853283110053 25.634401286763897 68-69 25.07141254541868 25.277255348976585 23.89613596767952 25.755196137925218 70-71 23.211773712616136 26.328663293073696 24.344176746214956 26.11538624809522 72-73 24.71040678688986 25.820616860744288 24.35005635915506 25.11891999321079 74-75 25.051871172568884 25.279773621693437 24.018473758147334 25.649881447590346 76-77 23.354075074139462 26.105312655058217 25.81221656800637 24.728395702795954 78-79 24.06084521754269 25.870968677914956 25.60692886883605 24.461257235706306 80-81 23.23089680419598 26.028117945556637 25.438331868705195 25.30265338154219 82-83 22.615009044969085 26.32509874749809 24.561940436831055 26.49795177070177 84-85 23.190901035337717 26.521473083239766 23.726898287521973 26.56072759390054 86-87 24.0344878463145 25.13905580997592 24.372404566101466 26.45405177760811 88-89 24.070785534530703 24.38130129503591 25.51899773609175 26.028915434341638 90-91 24.668004135380812 25.278015703579566 24.6601394761565 25.393840684883124 92-93 23.410425713038936 25.478113442402478 24.97220811365701 26.139252730901575 94-95 22.719984165959104 25.59822268627946 25.355405374394753 26.326387773366683 96-97 24.27533841445049 25.193840699232094 24.591880348009674 25.93894053830774 98-99 24.7470867315493 24.657502830851264 24.323677383290093 26.271733054309344 100 23.670456366925418 25.066978086144122 24.75641730209979 26.506148244830662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 622.0 1 667.5 2 669.5 3 529.0 4 371.0 5 282.5 6 256.0 7 263.0 8 278.0 9 293.5 10 310.0 11 354.0 12 422.5 13 484.5 14 577.5 15 703.5 16 816.5 17 929.5 18 1048.5 19 1150.0 20 1212.5 21 1275.0 22 1390.5 23 1543.0 24 1792.0 25 2059.5 26 2246.0 27 2472.5 28 2799.0 29 3076.5 30 3292.5 31 3600.0 32 3929.5 33 4305.0 34 4668.5 35 4938.0 36 5357.0 37 5884.5 38 6404.5 39 6866.5 40 7262.0 41 7612.0 42 8061.5 43 8504.0 44 8804.5 45 9366.5 46 10309.5 47 11713.5 48 13763.5 49 16869.5 50 20473.0 51 20599.5 52 17065.5 53 13926.0 54 12070.0 55 10808.0 56 9855.0 57 9014.0 58 8218.5 59 7541.5 60 6762.0 61 5828.5 62 4951.5 63 4136.5 64 3364.5 65 2752.0 66 2282.5 67 1890.5 68 1511.0 69 1173.5 70 942.0 71 756.5 72 570.0 73 463.5 74 377.0 75 303.5 76 233.5 77 170.0 78 119.0 79 64.5 80 34.5 81 22.5 82 16.0 83 8.5 84 6.5 85 4.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.8574954982206915 2 25.299000904636475 3 6.319042740506312 4 24.665755371457436 5 12.810451547727423 6 10.12878941375561 7 8.417685215043791 8 8.841751399047421 9 5.506582157829101 10-11 6.904202707060445 12-13 10.341964002887988 14-15 7.663298298883901 16-17 8.056401373221048 18-19 6.6970209778635175 20-21 8.232619992751493 22-23 5.659542773324353 24-25 5.421112560178302 26-27 9.960418586997777 28-29 6.3602793243478475 30-31 4.2319579244157675 32-33 8.908671668326594 34-35 7.337258180967248 36-37 7.097401096407994 38-39 6.0520750990962195 40-41 8.761703912766789 42-43 6.489553874383948 44-45 6.518233989789309 46-47 5.943775558834189 48-49 3.984823795649184 50-51 6.7291255846605615 52-53 4.56441896369183 54-55 5.111909524937432 56-57 4.92356249839477 58-59 3.6042771897482146 60-61 4.109104295739076 62-63 3.106156379399401 64-65 4.754478235930335 66-67 2.6843731896568945 68-69 5.241612136397492 70-71 2.5234220942477106 72-73 1.6416155608888836 74-75 0.7649457646175842 76-77 1.076717168402218 78-79 1.6471803594003713 80-81 2.090794681764869 82-83 0.6944583168054062 84-85 1.130082159255972 86-87 0.3899639572281025 88-89 0.6065630377521639 90-91 0.21445877340425834 92-93 0.26953600995385496 94-95 0.513959083035355 96-97 0.515385954448557 98-99 0.45146211513710804 100 0.2993576224897765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 350417.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.75568481021949 #Duplication Level Percentage of deduplicated Percentage of total 1 95.76331510223798 82.12248666289253 2 2.7414381208527607 4.7018780683713945 3 0.6426715240211606 1.653382099513862 4 0.24733166586844035 0.8484038552720197 5 0.1515838032352763 0.6499586426289341 6 0.08788404906152235 0.4521934086699466 7 0.058963021884102525 0.3539490024105819 8 0.04565625004434258 0.313222639073537 9 0.03907044718288198 0.30154616586086 >10 0.19633976960273788 3.002790392189314 >50 0.012195590216804098 0.6821208654509461 >100 0.010840524633559948 2.208467980309377 >500 0.0016938319739937418 1.0621098310985377 >1k 0.001016299184396245 1.647490386258153 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2556 0.7294166664288547 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1955 0.5579067225619762 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1160 0.3310341678628605 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 955 0.27253243992157916 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 809 0.23086779465608118 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 651 0.18577865799889845 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 637 0.1817834180419329 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 604 0.1723660667147998 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 500 0.14268714132019852 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 495 0.1412602699069965 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 488 0.13926264992851375 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 365 0.10416161316374492 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.85374282640397E-4 0.0 12-13 0.0 0.0 0.0 2.85374282640397E-4 0.0 14-15 0.0 0.0 0.0 2.85374282640397E-4 0.0 16-17 0.0 0.0 0.0 2.85374282640397E-4 0.0 18-19 0.0 0.0 0.0 5.70748565280794E-4 0.0 20-21 0.0 0.0 0.0 5.70748565280794E-4 0.0 22-23 0.0 0.0 0.0 5.70748565280794E-4 0.0 24-25 0.0 0.0 0.0 5.70748565280794E-4 0.0 26-27 0.0 0.0 0.0 0.001141497130561588 0.0 28-29 0.0 1.426871413201985E-4 0.0 0.01055884845769469 0.0 30-31 0.0 2.85374282640397E-4 0.0 0.03923896386305459 0.0 32-33 0.0 2.85374282640397E-4 0.0 0.10715804313146908 0.0 34-35 0.0 2.85374282640397E-4 0.0 0.21017815916465238 0.0 36-37 0.0 2.85374282640397E-4 0.0 0.36584983034498897 0.0 38-39 0.0 4.280614239605955E-4 0.0 0.5945773178812672 0.0 40-41 0.0 5.70748565280794E-4 0.0 0.9855400850986111 0.0 42-43 0.0 5.70748565280794E-4 0.0 1.2450879951600522 0.0 44-45 0.0 5.70748565280794E-4 0.0 1.5139105694073063 0.0 46-47 0.0 5.70748565280794E-4 0.0 1.85450477573862 0.0 48-49 0.0 5.70748565280794E-4 0.0 2.175836217991707 0.0 50-51 0.0 5.70748565280794E-4 0.0 2.504444704452124 0.0 52-53 0.0 5.70748565280794E-4 0.0 2.8926964159843847 0.0 54-55 0.0 5.70748565280794E-4 0.0 3.2750979547225163 0.0 56-57 0.0 5.70748565280794E-4 0.0 3.6816136203437617 0.0 58-59 0.0 5.70748565280794E-4 0.0 4.136072165448594 0.0 60-61 0.0 5.70748565280794E-4 0.0 4.566416583670312 0.0 62-63 0.0 5.70748565280794E-4 0.0 5.00318192325144 0.0 64-65 0.0 5.70748565280794E-4 0.0 5.50558334783986 0.0 66-67 0.0 5.70748565280794E-4 0.0 6.069054868913323 0.0 68-69 0.0 5.70748565280794E-4 0.0 6.705154144918768 0.0 70-71 0.0 5.70748565280794E-4 0.0 7.609647933747507 0.0 72-73 0.0 7.134357066009925E-4 0.0 8.357613928547988 0.0 74-75 0.0 8.56122847921191E-4 0.0 9.019254202849748 0.0 76-77 0.0 8.56122847921191E-4 0.0 9.798754055881993 0.0 78-79 0.0 8.56122847921191E-4 0.0 10.582534523153843 0.0 80-81 0.0 8.56122847921191E-4 0.0 11.432236449715624 0.0 82-83 0.0 8.56122847921191E-4 0.0 12.416064289118392 0.0 84-85 0.0 8.56122847921191E-4 0.0 13.314993279435644 0.0 86-87 0.0 8.56122847921191E-4 0.0 14.23347040811376 0.0 88 0.0 8.56122847921191E-4 0.0 15.04550292936701 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACCC 70 7.370383E-5 40.367027 3 TAATACT 60 0.0016812712 37.68313 4 CTGTGCG 55 0.0019356911 36.618637 9 TACATAG 130 1.1758239E-6 33.65791 2 GACTGAT 60 0.003059441 33.35579 7 GGATAAT 95 2.451773E-4 32.9057 1 GTATAGA 80 0.0042019566 31.260418 1 TTAGGCA 80 0.0068798047 28.26235 4 GTACATA 135 5.866823E-5 27.787035 1 GTCGGGA 95 0.009736999 26.318964 2 GGACTGA 100 0.0011849082 25.140879 6 GTACATT 200 1.1796747E-6 25.008333 1 ATATACT 140 1.491531E-4 24.22487 4 ACATAGG 140 1.493478E-4 24.220217 3 TAATGAT 105 0.001577615 23.935509 5 GAACCTT 130 1.9962063E-4 23.206964 6 TATTATA 170 2.8097274E-4 22.061485 2 ATGGGCG 165 4.0764084E-5 21.324362 5 ATACTGA 125 0.004332414 20.112703 6 TGGACTG 150 5.262644E-4 20.105827 5 >>END_MODULE