##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295069_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 350417 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.988296800668916 33.0 33.0 33.0 27.0 33.0 2 29.6757919849779 33.0 33.0 33.0 22.0 33.0 3 30.537665124694293 33.0 33.0 33.0 27.0 33.0 4 34.74634506887508 37.0 37.0 37.0 27.0 37.0 5 35.17060530739091 37.0 37.0 37.0 33.0 37.0 6 34.85479300376409 37.0 37.0 37.0 33.0 37.0 7 34.694897793200674 37.0 37.0 37.0 27.0 37.0 8 34.7062385671928 37.0 37.0 37.0 27.0 37.0 9 34.61671094724286 37.0 37.0 37.0 27.0 37.0 10-11 34.592267213063295 37.0 37.0 37.0 27.0 37.0 12-13 34.47400525659429 37.0 37.0 37.0 27.0 37.0 14-15 35.69904142778461 37.0 37.0 40.0 27.0 40.0 16-17 35.63840081959494 37.0 37.0 40.0 27.0 40.0 18-19 35.69619053870103 37.0 37.0 40.0 27.0 40.0 20-21 35.72254057308864 37.0 37.0 40.0 27.0 40.0 22-23 35.734372190846905 37.0 37.0 40.0 27.0 40.0 24-25 35.689263077989935 37.0 37.0 40.0 27.0 40.0 26-27 35.54929698045471 37.0 37.0 40.0 27.0 40.0 28-29 35.43559673189371 37.0 35.0 40.0 27.0 40.0 30-31 35.23986564578773 37.0 33.0 40.0 27.0 40.0 32-33 35.1130738520106 37.0 33.0 40.0 27.0 40.0 34-35 34.987873019859194 37.0 33.0 40.0 27.0 40.0 36-37 34.92318295059886 37.0 33.0 40.0 27.0 40.0 38-39 34.80568151659308 37.0 33.0 40.0 22.0 40.0 40-41 34.63090546406139 37.0 33.0 40.0 22.0 40.0 42-43 34.41766095822977 37.0 33.0 40.0 22.0 40.0 44-45 34.182114452209795 37.0 33.0 40.0 22.0 40.0 46-47 33.87985029265133 37.0 33.0 40.0 22.0 40.0 48-49 33.62133258375022 37.0 33.0 40.0 22.0 40.0 50-51 33.48595387780844 37.0 33.0 40.0 22.0 40.0 52-53 33.4281370481455 37.0 33.0 40.0 22.0 40.0 54-55 33.26753553623254 37.0 33.0 40.0 22.0 40.0 56-57 33.04755619733061 37.0 33.0 40.0 15.0 40.0 58-59 32.906554191149404 37.0 33.0 38.5 15.0 40.0 60-61 32.69122217244026 37.0 33.0 37.0 15.0 40.0 62-63 32.44203905632432 37.0 33.0 37.0 15.0 40.0 64-65 32.27115550900784 37.0 33.0 37.0 15.0 40.0 66-67 31.999148157766317 37.0 30.0 37.0 15.0 40.0 68-69 31.77107988482294 37.0 27.0 37.0 15.0 40.0 70-71 31.481990029022565 35.0 27.0 37.0 15.0 37.0 72-73 31.23747135555638 33.0 27.0 37.0 15.0 37.0 74-75 31.03631815808023 33.0 27.0 37.0 15.0 37.0 76-77 30.770591894799622 33.0 27.0 37.0 15.0 37.0 78-79 30.57050171652631 33.0 27.0 37.0 15.0 37.0 80-81 30.468814298393056 33.0 27.0 37.0 15.0 37.0 82-83 30.337131189411473 33.0 27.0 37.0 15.0 37.0 84-85 30.156044084619182 33.0 27.0 37.0 15.0 37.0 86-87 30.196548683425746 33.0 27.0 37.0 15.0 37.0 88-89 30.27275931247628 33.0 27.0 37.0 15.0 37.0 90-91 30.34310550001855 33.0 27.0 37.0 15.0 37.0 92-93 30.3176800783067 33.0 27.0 37.0 15.0 37.0 94-95 30.39629926630272 33.0 27.0 37.0 15.0 37.0 96-97 30.40355348056744 33.0 27.0 37.0 15.0 37.0 98-99 30.340796822071987 33.0 27.0 37.0 15.0 37.0 100 29.692631921396508 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6361274933807195 1101 2 4.01883898282702 1101 3 2.402132187279811 1101 4 1.3211464550737801 1101 5 0.6426916581731632 1101 6 0.6930070594050477 1101 7 0.7814788378465778 1101 8 0.7092724621642148 1101 9 0.8977150888582344 1101 10-11 0.8968201465809145 1101 12-13 0.694081783126876 1101 14-15 1.1914714232076236 1101 16-17 1.149718654793162 1101 18-19 1.0080594940222696 1101 20-21 1.1778639083607558 1101 22-23 1.1335666604110628 1101 24-25 1.0340818720036467 1101 26-27 1.1503847768109168 1101 28-29 0.99799492514218 1101 30-31 0.552312264221861 1101 32-33 0.8358658041785674 1101 34-35 0.931273910037234 1101 36-37 0.9731758666505215 1101 38-39 1.187824846449324 1101 40-41 0.8805945848719006 1101 42-43 1.1872480126013514 1101 44-45 1.0273808101731987 1101 46-47 1.099006914108024 1101 48-49 1.0157752891602527 1101 50-51 0.8445656602536857 1101 52-53 0.9032466040144911 1101 54-55 1.0298868014413571 1101 56-57 1.0814672239591943 1101 58-59 0.8848898260084255 1101 60-61 1.024317356746188 1101 62-63 0.8709235070655907 1101 64-65 1.2633692781983328 1101 66-67 1.0768075321332766 1101 68-69 0.9759679327502013 1101 70-71 1.1439299859928944 1101 72-73 0.9726765289938015 1101 74-75 0.6436344711165809 1101 76-77 0.9391438268725807 1101 78-79 0.8698707892868569 1101 80-81 0.842900292499845 1101 82-83 0.823303964701811 1101 84-85 0.9835304834718066 1101 86-87 1.0794707740685183 1101 88-89 1.0311025386199368 1101 90-91 0.8674745575131197 1101 92-93 0.6376639699035209 1101 94-95 0.40173884392136117 1101 96-97 0.6839789574090425 1101 98-99 0.540714703511675 1101 100 0.6328377505817677 1102 1 0.8707548744657139 1102 2 4.049062052894971 1102 3 2.267440753616004 1102 4 1.2952399718388463 1102 5 0.680789018092959 1102 6 0.5610100113934635 1102 7 0.8954715414223813 1102 8 0.6032097463348762 1102 9 0.9132756881675803 1102 10-11 0.7832973939909706 1102 12-13 0.8536075155541951 1102 14-15 0.9224749600239335 1102 16-17 1.0888512993291997 1102 18-19 0.9134461766556683 1102 20-21 0.9652580823609753 1102 22-23 0.9085471661479403 1102 24-25 1.2556550590371742 1102 26-27 1.0779078914371851 1102 28-29 0.9147927139357606 1102 30-31 1.0324141913690141 1102 32-33 1.003568683759724 1102 34-35 1.099683456656372 1102 36-37 1.0220646936528581 1102 38-39 1.2321847383954108 1102 40-41 0.8786834508627663 1102 42-43 1.2367669220365727 1102 44-45 1.2512480542413726 1102 46-47 1.091059520769491 1102 48-49 0.9279014170426194 1102 50-51 0.8392256246070318 1102 52-53 0.9363659646026576 1102 54-55 0.655140923085554 1102 56-57 0.948944527067134 1102 58-59 0.9753049145680777 1102 60-61 0.7126563062838969 1102 62-63 0.8674215019413793 1102 64-65 1.2535281564427407 1102 66-67 1.2317416693728909 1102 68-69 1.2243144015036833 1102 70-71 0.8780978594472764 1102 72-73 1.0517988694193328 1102 74-75 0.9825317920478476 1102 76-77 0.7705358565038587 1102 78-79 0.7598813718868342 1102 80-81 0.7923126797116105 1102 82-83 1.2135094899329566 1102 84-85 1.0684036315168122 1102 86-87 0.8239775692116567 1102 88-89 0.6549259205961029 1102 90-91 0.9642078759936794 1102 92-93 0.9817760062185457 1102 94-95 0.7850830847590551 1102 96-97 0.7965722513825284 1102 98-99 0.9819186135038791 1102 100 0.8006206958864368 1103 1 0.6418196735409829 1103 2 3.907976175421659 1103 3 2.367903118476274 1103 4 1.3553168059994505 1103 5 0.5799118847550915 1103 6 0.5166841801566378 1103 7 0.42840823823343044 1103 8 0.6683011993844445 1103 9 0.6254195652329244 1103 10-11 0.6275382316873035 1103 12-13 0.8464408602199924 1103 14-15 1.066120842936833 1103 16-17 0.7097784088688712 1103 18-19 0.9643233099935316 1103 20-21 0.8955310816130364 1103 22-23 0.7532129715467306 1103 24-25 0.8096310568613418 1103 26-27 0.7915425552027386 1103 28-29 1.1192939718401576 1103 30-31 1.0580873402097026 1103 32-33 1.0062391808019413 1103 34-35 1.1020496768236328 1103 36-37 1.1254831338514109 1103 38-39 0.9567682700470002 1103 40-41 1.252750221788176 1103 42-43 1.6706878440465047 1103 44-45 1.2755001110850515 1103 46-47 1.0232176767496313 1103 48-49 0.9496019678007599 1103 50-51 0.7310985422652934 1103 52-53 0.9171489250976563 1103 54-55 0.9094857928754507 1103 56-57 0.8618295478055629 1103 58-59 0.8217283863842226 1103 60-61 0.8426114296945144 1103 62-63 0.8588932500885207 1103 64-65 0.7331382060789622 1103 66-67 0.9450601571678199 1103 68-69 0.8462342355962917 1103 70-71 1.132379834017211 1103 72-73 1.0712793888998569 1103 74-75 0.8499673669441563 1103 76-77 0.8869039125316043 1103 78-79 0.8808475784551391 1103 80-81 0.8484543129474851 1103 82-83 1.2157397204354403 1103 84-85 1.1403676622504761 1103 86-87 1.1975938743724655 1103 88-89 0.836169546080562 1103 90-91 0.9124179120309392 1103 92-93 0.7089008447308203 1103 94-95 0.8469498828824022 1103 96-97 1.0551243014267264 1103 98-99 1.0671688344870844 1103 100 1.0195640561910508 1104 1 0.6459020593554392 1104 2 3.5905377275366597 1104 3 2.201382899858629 1104 4 1.1577841627293282 1104 5 0.3537445473563636 1104 6 0.39823875548658094 1104 7 0.3812560669946521 1104 8 0.49585510691128576 1104 9 0.5057949408721711 1104 10-11 0.5260497337170733 1104 12-13 0.2888709605662001 1104 14-15 0.5251625454832691 1104 16-17 0.6030942270155393 1104 18-19 0.3392727854166253 1104 20-21 0.6502478803758365 1104 22-23 0.505791921689962 1104 24-25 0.6438963855786284 1104 26-27 0.6380190151433425 1104 28-29 0.7114437706205408 1104 30-31 0.5743158343137011 1104 32-33 0.7053262236643576 1104 34-35 0.6191805838056936 1104 36-37 0.6277694807926935 1104 38-39 0.6977388691366784 1104 40-41 0.4593588364482173 1104 42-43 0.5471012141675899 1104 44-45 0.657686463298468 1104 46-47 0.7900797051136905 1104 48-49 0.5624896890105404 1104 50-51 0.5620371571010665 1104 52-53 0.5910633057102785 1104 54-55 0.5391872145931131 1104 56-57 0.37868366797497544 1104 58-59 0.4186626429260585 1104 60-61 0.36000945791798244 1104 62-63 0.5755110031068185 1104 64-65 0.6088521403179357 1104 66-67 0.5699507604751979 1104 68-69 0.45402644338849996 1104 70-71 0.30425302172260515 1104 72-73 0.21959318606344524 1104 74-75 0.4508144224596826 1104 76-77 0.31302907289221693 1104 78-79 0.6476577642098427 1104 80-81 0.3784140690236164 1104 82-83 0.45766106025935116 1104 84-85 0.32994905514716066 1104 86-87 0.4883632538427918 1104 88-89 0.04724892678105164 1104 90-91 0.2727231683443172 1104 92-93 0.2977089919286833 1104 94-95 0.42842186151269956 1104 96-97 0.3961151103471714 1104 98-99 0.4386017993571265 1104 100 0.3606884257321994 1105 1 0.4028109132723827 1105 2 3.6674505593054896 1105 3 2.120740831943909 1105 4 0.9867276523709307 1105 5 0.27032331059958636 1105 6 0.48670156434724987 1105 7 0.4215602305980184 1105 8 0.5340407092184378 1105 9 0.6213462107658643 1105 10-11 0.1760946621335009 1105 12-13 0.3321807109204755 1105 14-15 0.6457438446070753 1105 16-17 0.6143920367108464 1105 18-19 0.5332294694508732 1105 20-21 0.3896630651150943 1105 22-23 0.41034389836269725 1105 24-25 0.5073779294113621 1105 26-27 0.5842514211399532 1105 28-29 0.6040027014307796 1105 30-31 0.6552189487586304 1105 32-33 0.39287039735442164 1105 34-35 0.44074995292549346 1105 36-37 0.5611582199201948 1105 38-39 0.6167300930009532 1105 40-41 0.3734203652929722 1105 42-43 0.5709230419194995 1105 44-45 0.6398420683403927 1105 46-47 0.791513685203121 1105 48-49 0.9496820714241707 1105 50-51 0.6551739419312455 1105 52-53 0.5233288344271756 1105 54-55 0.48438608942929307 1105 56-57 0.3072465979470209 1105 58-59 0.44610715721968575 1105 60-61 0.653353836893622 1105 62-63 0.5144323304307505 1105 64-65 0.40584673114969405 1105 66-67 0.3727061332389887 1105 68-69 0.3556012465381073 1105 70-71 0.4748620237434942 1105 72-73 0.44418732086290547 1105 74-75 0.46025471738898815 1105 76-77 0.20246787612706285 1105 78-79 0.4341403486770403 1105 80-81 0.5332448504939258 1105 82-83 0.4452604621609062 1105 84-85 0.5675671032294467 1105 86-87 0.20381809634471892 1105 88-89 0.6330284614434163 1105 90-91 0.6938134194106986 1105 92-93 0.5579413055823039 1105 94-95 0.5678313636063983 1105 96-97 0.6591772039048251 1105 98-99 0.669082799786878 1105 100 0.41672463839100615 1106 1 0.5992341544848756 1106 2 3.8508842477414937 1106 3 2.273182806108906 1106 4 1.059348721796077 1106 5 0.6080928541125346 1106 6 0.5123976170465454 1106 7 0.4504522073362196 1106 8 0.39517497919939615 1106 9 0.5913477546905241 1106 10-11 0.47982581338664687 1106 12-13 0.5476122378619621 1106 14-15 0.7501671887478807 1106 16-17 0.6888761085548083 1106 18-19 0.6358155432504731 1106 20-21 0.6770903719958525 1106 22-23 0.36810983938477193 1106 24-25 0.3521826229423155 1106 26-27 0.2688662119381604 1106 28-29 0.39899009271275077 1106 30-31 0.3689650246167915 1106 32-33 0.5743870460086313 1106 34-35 0.5961588356386827 1106 36-37 0.608150140047826 1106 38-39 0.6311889474088517 1106 40-41 0.14372297452563743 1106 42-43 0.5938734819380258 1106 44-45 0.6753600550626402 1106 46-47 0.7288071852803242 1106 48-49 0.38110608459899 1106 50-51 0.48415855414883424 1106 52-53 0.3120092934874421 1106 54-55 0.5620789513509621 1106 56-57 0.7106921231387204 1106 58-59 0.5143563466592482 1106 60-61 0.9699690394050648 1106 62-63 0.6687141481447085 1106 64-65 0.6538113238109062 1106 66-67 0.768398583447425 1106 68-69 0.4906277505780423 1106 70-71 0.6433042037651084 1106 72-73 0.6367661896807704 1106 74-75 0.3710776292308857 1106 76-77 0.5361357264693005 1106 78-79 0.7185184043612551 1106 80-81 0.6870248926945344 1106 82-83 0.9170426658560338 1106 84-85 0.8253562031213697 1106 86-87 0.849834770731011 1106 88-89 0.5592957662656062 1106 90-91 0.427633089010822 1106 92-93 0.5092021773850917 1106 94-95 0.5302110661435826 1106 96-97 0.6221899155511643 1106 98-99 0.5269388030805828 1106 100 0.3767833075279512 1107 1 0.23819879111215414 1107 2 2.9542993886322897 1107 3 1.9197135596906918 1107 4 0.8967118348116969 1107 5 0.24968052670916308 1107 6 0.08643562076900224 1107 7 0.16693924365184643 1107 8 -0.25729439707208 1107 9 0.06489664444509913 1107 10-11 0.10435942374028429 1107 12-13 0.013577696893257496 1107 14-15 0.050738543117446966 1107 16-17 -0.07296434506593386 1107 18-19 0.004157317046079356 1107 20-21 0.008350381518418715 1107 22-23 -0.09819323813762537 1107 24-25 0.09261892991543874 1107 26-27 0.1656588362726623 1107 28-29 -0.0245436474941485 1107 30-31 0.1315499702948486 1107 32-33 0.12796017503949741 1107 34-35 0.38016957017531894 1107 36-37 -0.07091318506147104 1107 38-39 -0.16469470406497777 1107 40-41 0.18292567547356953 1107 42-43 -0.06744323017728249 1107 44-45 0.33931278847388313 1107 46-47 -0.09356653241868429 1107 48-49 -0.497680220209908 1107 50-51 0.10761871083844454 1107 52-53 0.02934037603083084 1107 54-55 0.12365969844008617 1107 56-57 0.20108730991100998 1107 58-59 0.12713390801490476 1107 60-61 0.11385078636433121 1107 62-63 0.2695408806037065 1107 64-65 0.05227039838996461 1107 66-67 0.03388358539521974 1107 68-69 0.10833107915009066 1107 70-71 0.25962675927737777 1107 72-73 -0.12038656104424561 1107 74-75 0.06711710462369425 1107 76-77 0.17033041979916774 1107 78-79 0.08343638038168777 1107 80-81 0.17695693615209152 1107 82-83 -0.11738576335229567 1107 84-85 0.2609619686063702 1107 86-87 0.22791553946234444 1107 88-89 0.1985201094245035 1107 90-91 0.15866801953932708 1107 92-93 0.26544595524944015 1107 94-95 -0.006580692502478058 1107 96-97 0.01991315495730106 1107 98-99 0.058125229085863594 1107 100 -0.2442529215332101 1108 1 0.5799206454167916 1108 2 3.686749719758115 1108 3 2.2998460100218168 1108 4 1.0066456096461351 1108 5 0.3132566856495629 1108 6 0.19107138235840893 1108 7 -0.0025971801892126223 1108 8 -0.08510896660850165 1108 9 -0.23046759396549277 1108 10-11 -0.008885609372299541 1108 12-13 0.3062929286813372 1108 14-15 0.13550675503797294 1108 16-17 0.09524757546386553 1108 18-19 0.2339586415493926 1108 20-21 0.31232389145220196 1108 22-23 0.15015113272330893 1108 24-25 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1.0422287186960268 2202 90-91 1.0083368937115118 2202 92-93 1.201207544653414 2202 94-95 0.9848100359743412 2202 96-97 0.9536879893943855 2202 98-99 0.9548139708077166 2202 100 0.8325682705197686 2203 1 0.17854015253358924 2203 2 3.877651414317505 2203 3 2.2704147415955624 2203 4 1.1422646006090673 2203 5 0.5015128729346827 2203 6 0.6486181642702746 2203 7 0.5996703974744477 2203 8 0.5398361373658815 2203 9 0.8537302241908762 2203 10-11 0.7352000546727737 2203 12-13 0.7850536344998744 2203 14-15 0.9379414265804016 2203 16-17 0.9955450200648173 2203 18-19 1.058376218775635 2203 20-21 0.9324153994088391 2203 22-23 1.036079378587516 2203 24-25 0.9585545244109639 2203 26-27 1.1002568923847065 2203 28-29 1.0536606619532165 2203 30-31 1.0473022864259676 2203 32-33 1.0389288821178013 2203 34-35 1.4476020108832373 2203 36-37 1.505568570211615 2203 38-39 1.262346509508994 2203 40-41 0.8743417742251722 2203 42-43 0.9985824725355812 2203 44-45 1.2966644817210167 2203 46-47 1.2190136245270864 2203 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2210 88-89 0.16208550004529698 2210 90-91 0.15766656581617866 2210 92-93 0.23271564781391874 2210 94-95 0.4266880309132297 2210 96-97 0.33554875502191095 2210 98-99 0.10610347708026424 2210 100 -0.22522530079342573 2211 1 0.690737422458735 2211 2 4.007326824772306 2211 3 2.3410632916364484 2211 4 1.2622432129258598 2211 5 0.3515569064001127 2211 6 0.6558616693341222 2211 7 0.5247285845537704 2211 8 0.45899384007708477 2211 9 0.3122161020294598 2211 10-11 0.5358715650206349 2211 12-13 0.36841055718969073 2211 14-15 0.45464898146497035 2211 16-17 0.4387827378732041 2211 18-19 0.6236306693008942 2211 20-21 0.6550548911368423 2211 22-23 0.6097064780402377 2211 24-25 0.6420985893288744 2211 26-27 0.4086421223023038 2211 28-29 0.4591208116669989 2211 30-31 0.648563530685891 2211 32-33 0.3498538994166651 2211 34-35 0.642634092408052 2211 36-37 0.511954995323272 2211 38-39 0.5477855860643146 2211 40-41 0.3124904815284424 2211 42-43 0.5697138180724792 2211 44-45 0.7576429682279056 2211 46-47 1.069213347745304 2211 48-49 0.3330435262423208 2211 50-51 0.38791672408347466 2211 52-53 1.0975601805089141 2211 54-55 1.009161117550775 2211 56-57 0.9441888551648674 2211 58-59 1.0448885595443969 2211 60-61 0.750944763027853 2211 62-63 0.5979625141577856 2211 64-65 0.5808654788062384 2211 66-67 0.38261426972193036 2211 68-69 0.5064073957694504 2211 70-71 0.09666554830292284 2211 72-73 0.5648941075145721 2211 74-75 0.8450972370740253 2211 76-77 0.6080078086354987 2211 78-79 0.8767350243425867 2211 80-81 0.6983460878392584 2211 82-83 0.2949604996562165 2211 84-85 0.7180732899561058 2211 86-87 0.39521292199151503 2211 88-89 0.801429286340305 2211 90-91 1.1428291674421942 2211 92-93 0.9770826629126361 2211 94-95 0.7341793201118385 2211 96-97 0.8821805784830055 2211 98-99 0.8914112587295122 2211 100 0.45787574450273993 2212 1 0.2555172966725685 2212 2 4.129833563316783 2212 3 2.5657712880425585 2212 4 1.0157921796823715 2212 5 0.24558206363281698 2212 6 0.19516984543475502 2212 7 0.5311526636193591 2212 8 0.33585817071499946 2212 9 0.17425563482370876 2212 10-11 0.24451936376277672 2212 12-13 0.35023894892374585 2212 14-15 0.5617469149780021 2212 16-17 0.2965096022038409 2212 18-19 0.25620030092713364 2212 20-21 0.5980917285133032 2212 22-23 0.5155016262882128 2212 24-25 0.3822603143962553 2212 26-27 0.31951364072099153 2212 28-29 0.2880516292770636 2212 30-31 0.13531105988624859 2212 32-33 0.24565354002762518 2212 34-35 0.5939144158581868 2212 36-37 0.21139344995039977 2212 38-39 0.47606501104185384 2212 40-41 0.2169379208815414 2212 42-43 0.22139845419108184 2212 44-45 0.17428717307965513 2212 46-47 0.20387192815859834 2212 48-49 -0.05165997780080289 2212 50-51 -0.05123421931275374 2212 52-53 0.6601163350462045 2212 54-55 -0.0485882085947793 2212 56-57 0.08208193692587429 2212 58-59 0.027008954872336233 2212 60-61 -0.3204613465858088 2212 62-63 0.11667320598168374 2212 64-65 0.3512832685636482 2212 66-67 0.07805452129425916 2212 68-69 -0.03842638680017885 2212 70-71 -0.3843317562792876 2212 72-73 -0.43121999850519543 2212 74-75 -0.29877068745427593 2212 76-77 -0.00782777087932729 2212 78-79 -0.3658076531057546 2212 80-81 -0.5302028789172546 2212 82-83 -0.23455432514162666 2212 84-85 0.01070581466410303 2212 86-87 -0.16507458923939566 2212 88-89 -0.07384025543867168 2212 90-91 0.3165713058069741 2212 92-93 0.38543845806088584 2212 94-95 0.08534733448739829 2212 96-97 0.08465137812361334 2212 98-99 0.5355711868517012 2212 100 -0.1670568700525159 2213 1 -0.6771823770400118 2213 2 -25.556958889513407 2213 3 -14.475227435180594 2213 4 -6.2931452582520855 2213 5 -2.221250111143746 2213 6 -1.3943889572322945 2213 7 -0.9954468540427186 2213 8 -0.9042893679429653 2213 9 -0.8239994368176582 2213 10-11 -0.8047925953803698 2213 12-13 -1.0993212723842376 2213 14-15 -1.0809399909661082 2213 16-17 -1.448560620522791 2213 18-19 -1.2784996565136453 2213 20-21 -1.2086042567328334 2213 22-23 -1.864353672335703 2213 24-25 -1.8124126888666154 2213 26-27 -1.3051423388505796 2213 28-29 -1.4787237998618252 2213 30-31 -1.3840179229481961 2213 32-33 -1.0741435943176754 2213 34-35 -0.8861253060881893 2213 36-37 -1.4552755811178386 2213 38-39 -1.4826028851136286 2213 40-41 -1.3529230523061457 2213 42-43 -1.262804978920002 2213 44-45 -1.7072066558322447 2213 46-47 -1.2293455494978005 2213 48-49 -1.1975955715020419 2213 50-51 -1.602872749600735 2213 52-53 -1.5592693934259216 2213 54-55 -0.9921546719229113 2213 56-57 -1.1581294405744806 2213 58-59 -1.1766653251673844 2213 60-61 -1.069042705643831 2213 62-63 -1.0779146788246692 2213 64-65 -0.9584202354794762 2213 66-67 -1.494516056092607 2213 68-69 -1.2546201731528512 2213 70-71 -1.8048382110955714 2213 72-73 -1.6718853411069823 2213 74-75 -1.6671208080387565 2213 76-77 -2.0117290334697664 2213 78-79 -1.7286534468353594 2213 80-81 -1.2246488996294111 2213 82-83 -1.7509041619979158 2213 84-85 -1.5000971361090585 2213 86-87 -1.5738346970561068 2213 88-89 -1.573570713659695 2213 90-91 -1.7128725869437673 2213 92-93 -1.349233126561046 2213 94-95 -1.035996118484654 2213 96-97 -1.7168169152513322 2213 98-99 -1.470493503175252 2213 100 -1.0176881652716965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 2.0 5 5.0 6 7.0 7 6.0 8 7.0 9 12.0 10 78.0 11 327.0 12 1098.0 13 1750.0 14 2490.0 15 3000.0 16 3410.0 17 3439.0 18 3414.0 19 3758.0 20 4239.0 21 5003.0 22 5517.0 23 5417.0 24 5188.0 25 4948.0 26 5110.0 27 5728.0 28 6492.0 29 7645.0 30 8933.0 31 10926.0 32 14065.0 33 18308.0 34 24084.0 35 33884.0 36 50390.0 37 72242.0 38 39391.0 39 101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.98836874532159 18.441584614498762 9.012782864052514 31.557263776127137 2 13.890278824415974 22.565184626978148 31.014619442351165 32.52991710625471 3 18.514798083426317 25.981045440147028 27.02209082321919 28.482065653207467 4 12.643622395104995 18.66677321217587 29.85690557594507 38.83269881677407 5 12.42993886353048 36.992280055906 33.25520974753696 17.322571333026556 6 34.68633738111485 32.195709713248036 16.490450642857756 16.627502262779352 7 26.30482133732233 31.88808354053846 21.6556236564972 20.15147146564201 8 28.690883345418833 31.880884544452755 19.89739695445384 19.53083515567457 9 27.241947317485376 13.943709015749942 20.106369919523136 38.707973747241546 10-11 24.820358512165946 25.675100847605208 25.61243687211125 23.892103768117597 12-13 27.23274695534506 23.383178317146562 26.409990693319173 22.974084034189204 14-15 26.247114795798197 23.567532691843777 24.008324804405635 26.177027707952387 16-17 22.917677687507492 26.61444565608354 28.007085234052514 22.460791422356454 18-19 24.292157282523373 25.09997859130807 29.489616784414473 21.118247341754085 20-21 22.980434804326634 24.700898979995234 29.561156757049904 22.757509458628224 22-23 22.122049834375414 24.58865018460612 27.562756803738075 25.726543177280387 24-25 22.8540480060739 25.56047283941984 26.89498974601222 24.69048940849404 26-27 24.043450712667035 23.50802285557718 28.308036555223847 24.140489876531934 28-29 22.765150055586712 23.091820118822845 30.07091409105375 24.072115734536688 30-31 23.72191466253352 24.65444134743969 28.309620552694035 23.31402343733275 32-33 21.206353101995795 24.568114916319814 28.880473386290117 25.34505859539427 34-35 21.035110599959474 25.34780983256613 29.761249061805273 23.855830505669122 36-37 24.271767079619753 24.32456343124916 28.190255220417633 23.213414268713453 38-39 24.2986893456717 23.52385505033569 28.082534835436896 24.09492076855571 40-41 22.420268554060417 24.420154397180323 28.76639221450078 24.39318483425848 42-43 24.39699937356144 23.885717058107527 28.45229720300177 23.264986365329264 44-45 25.153044188031014 23.30409915836399 27.116005354049356 24.426851299555636 46-47 22.939494181748643 24.33090822946487 29.83990889448943 22.889688694297057 48-49 24.419167231598102 24.905555357334 28.06108395475791 22.614193456309987 50-51 22.275026937540584 25.35396998694154 27.912715232733216 24.458287842784664 52-53 21.781364268240814 25.050452862611216 26.096475578530914 27.071707290617052 54-55 22.75549859493434 25.60202318037218 25.50083561802195 26.141642606671528 56-57 23.542750642893633 23.687169812397787 26.409157202834727 26.360922341873856 58-59 22.636148539690783 23.22423944904116 28.071958010223447 26.067654001044605 60-61 23.46551438731884 24.435181432624194 26.756816923436972 25.342487256619993 62-63 21.276544126041784 24.84173421623473 26.947282794839595 26.93443886288389 64-65 20.667903525046384 24.983159697445412 27.834165834165837 26.51477094334237 66-67 23.466020830776344 24.215848858417512 26.560524280213617 25.757606030592527 68-69 23.87856903281976 23.721285387036232 26.058988503450394 26.341157076693616 70-71 21.89590897673038 24.283243132150442 26.816726039597178 27.004121851522 72-73 23.43319389675985 23.892749440897877 26.889708096598003 25.784348565744274 74-75 24.063214051753544 23.228009882012426 26.018636527765686 26.690139538468344 76-77 21.97857871714916 24.217894834488124 28.49958256327556 25.30394388508716 78-79 22.95106531759736 24.250139140610504 27.488476303283722 25.31031923850841 80-81 21.368206843602923 24.631604784642562 27.553792979441795 26.44639539231272 82-83 20.69277980966678 24.48032238777004 26.31373158992025 28.513166212642933 84-85 21.237409989810978 24.752338195714902 25.63909731517765 28.371154499296473 86-87 22.129673760727425 23.341904808112172 26.555208954670125 27.973212476490282 88-89 21.371033911970148 22.894753740448213 27.73147256291159 28.002739784670048 90-91 21.890246251714142 23.70320796475962 26.9023255282989 27.504220255227334 92-93 20.215096111175324 23.9911929600758 26.985900459900996 28.807810468847876 94-95 19.49570575082653 24.22430263933301 27.843486425207008 28.436505184633454 96-97 21.47971530655343 23.543714256361085 26.991370158556215 27.985200278529277 98-99 21.523193927200605 23.13385557125123 26.747856113465463 28.595094388082703 100 20.08598280343931 23.87151141200331 27.778158653983485 28.264347130573885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 89.0 1 73.0 2 44.5 3 27.0 4 25.0 5 71.5 6 228.0 7 426.0 8 590.0 9 624.0 10 436.0 11 254.0 12 194.5 13 182.5 14 189.5 15 203.0 16 217.0 17 218.5 18 229.0 19 247.5 20 278.0 21 347.5 22 399.5 23 472.5 24 625.5 25 794.5 26 968.5 27 1183.0 28 1435.0 29 1672.5 30 2028.5 31 2458.0 32 2849.0 33 3266.0 34 3693.0 35 4189.5 36 4800.5 37 5339.0 38 5821.5 39 6354.0 40 6858.0 41 7319.0 42 7763.0 43 8394.5 44 8913.5 45 9140.0 46 10021.0 47 11755.0 48 13729.0 49 17514.5 50 28244.5 51 30673.5 52 19988.0 53 13407.0 54 11565.5 55 10973.0 56 10615.0 57 9693.0 58 8940.5 59 8454.5 60 7691.0 61 6795.5 62 5768.0 63 4677.5 64 3595.0 65 2902.5 66 2497.0 67 2152.0 68 1704.0 69 1268.5 70 923.5 71 703.5 72 570.5 73 471.5 74 406.5 75 326.0 76 201.5 77 142.5 78 99.0 79 34.5 80 14.5 81 5.5 82 1.5 83 0.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8780966676845016 2 5.327081734048291 3 0.0 4 0.005422111370167543 5 0.15524360975637597 6 0.052508868005833056 7 0.03424491391684764 8 0.038525528156453596 9 0.037098656743251614 10-11 0.03881090243909399 12-13 0.03738403102589201 14-15 0.03952433814569499 16-17 0.03167654537308407 18-19 0.026254434002916528 20-21 0.021545758339349973 22-23 0.021831132621990372 24-25 0.018692015512946004 26-27 0.012271094153537072 28-29 0.01812126694766521 30-31 0.022401881187271166 32-33 0.0015695585545221836 34-35 0.0028537428264039703 36-37 0.003995239956965558 38-39 0.005564798511487742 40-41 0.006278234218088734 42-43 0.0067062956420493305 44-45 0.007990479913931117 46-47 0.01541021126258144 48-49 0.02268725546991156 50-51 0.019833512643507592 52-53 0.02568368543763573 54-55 0.022116506904630767 56-57 0.013697965566739058 58-59 0.013127217001458264 60-61 0.011129597022975484 62-63 0.016266334110502628 64-65 0.0191200769369066 66-67 0.01997619978482779 68-69 0.02696786970951752 70-71 0.025398311154995336 72-73 0.022401881187271166 74-75 0.02382875260047315 76-77 0.018406641230305606 78-79 0.014839462697300644 80-81 0.013127217001458264 82-83 0.008989289903172505 84-85 0.011414971305615881 86-87 0.00856122847921191 88-89 0.0069916699246897265 90-91 0.0067062956420493305 92-93 0.004423301380926154 94-95 0.0018549328371625805 96-97 0.0014268714132019851 98-99 5.70748565280794E-4 100 0.09902487607621777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 350417.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.79292160009999 #Duplication Level Percentage of deduplicated Percentage of total 1 84.08205924484287 50.27511976401852 2 9.697700401921121 11.597076796666556 3 2.8870147168421822 5.178691338674384 4 1.145487762336939 2.7396823986914622 5 0.5672283554417108 1.6958120293139929 6 0.33633906227423327 1.2066417108968628 7 0.22172103099580837 0.9280143756397996 8 0.15731934669676778 0.7525266690575029 9 0.11799669224479621 0.6349830271617803 >10 0.6967209791877194 7.7159222970372605 >50 0.05453446784449798 2.278538151507233 >100 0.022005136147778947 2.5356817365190785 >500 0.007653960399227461 3.338004045705318 >1k 0.005262097774468879 5.2884624508179625 >5k 9.567450499034326E-4 3.8348432082923054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8303 2.3694626687632163 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5104 1.4565503385965863 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2945 0.8404272623759692 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2406 0.6866105240327952 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2185 0.6235428075692675 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1917 0.5470624998216411 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1877 0.5356475285160252 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1379 0.3935311357611075 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1294 0.36927432173667374 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1262 0.36014234469218104 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1098 0.3133409623391559 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1063 0.30335286244674203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.30335286244674203 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 906 0.25854910007219967 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 875 0.24970249731034738 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 873 0.2491317487450666 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 864 0.24656338020130303 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 841 0.23999977170057388 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 829 0.23657528030888914 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 761 0.21716982908934215 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 734 0.20946472345805142 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 688 0.19633750645659315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 0.19576675789131234 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 668 0.1906300208037852 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 653 0.18634940656417923 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 615 0.17550518382384417 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 572 0.1632340896703071 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 567 0.16180721825710512 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 538 0.15353136406053358 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.13127217001458263 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 460 0.13127217001458263 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 392 0.11186671879503562 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 354 0.10102249605470054 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.85374282640397E-4 0.0 12-13 0.0 0.0 0.0 2.85374282640397E-4 0.0 14-15 0.0 0.0 0.0 2.85374282640397E-4 0.0 16-17 0.0 0.0 0.0 2.85374282640397E-4 0.0 18-19 0.0 0.0 0.0 5.70748565280794E-4 0.0 20-21 0.0 0.0 0.0 5.70748565280794E-4 0.0 22-23 0.0 0.0 0.0 5.70748565280794E-4 0.0 24-25 0.0 0.0 0.0 8.56122847921191E-4 0.0 26-27 0.0 0.0 0.0 0.0021403071198029776 0.0 28-29 0.0 0.0 0.0 0.014982149838620843 0.0 30-31 0.0 0.0 0.0 0.056789482245439005 0.0 32-33 0.0 0.0 0.0 0.15724122973485877 0.0 34-35 0.0 0.0 0.0 0.3238998107968506 0.0 36-37 0.0 0.0 0.0 0.5597616553991387 0.0 38-39 0.0 0.0 0.0 0.902781543132896 0.0 40-41 0.0 0.0 0.0 1.4536965957701824 0.0 42-43 0.0 0.0 0.0 1.8440886144222455 0.0 44-45 0.0 0.0 0.0 2.244896794390683 0.0 46-47 0.0 0.0 0.0 2.7504373360881464 0.0 48-49 0.0 0.0 0.0 3.2214475895861217 0.0 50-51 0.0 0.0 0.0 3.6977372673129443 0.0 52-53 0.0 0.0 0.0 4.259353855549246 0.0 54-55 0.0 0.0 0.0 4.810696969610492 0.0 56-57 0.0 0.0 0.0 5.405559661774401 0.0 58-59 0.0 0.0 0.0 6.065345003238998 0.0 60-61 0.0 0.0 0.0 6.687032877971102 0.0 62-63 0.0 0.0 0.0 7.312573305518853 0.0 64-65 0.0 0.0 0.0 8.035140989164338 0.0 66-67 0.0 0.0 0.0 8.801798999477764 0.0 68-69 0.0 0.0 0.0 9.66805263443269 0.0 70-71 0.0 0.0 0.0 10.887885005579067 0.0 72-73 0.0 0.0 0.0 11.879846012037087 0.0 74-75 0.0 0.0 0.0 12.767502718190041 0.0 76-77 0.0 0.0 0.0 13.809404224110132 0.0 78-79 0.0 0.0 0.0 14.86329144990112 0.0 80-81 0.0 0.0 0.0 15.980531766438272 0.0 82-83 0.0 0.0 0.0 17.260863485504416 0.0 84-85 0.0 0.0 0.0 18.43731896568945 0.0 86-87 0.0 0.0 0.0 19.62361985862558 0.0 88 0.0 0.0 0.0 20.669659291643956 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTACGG 30 0.008651998 49.665836 2 ACTAAGC 45 0.0010183131 41.733295 8 CTCGAAC 55 0.0027286555 34.145424 8 CCCTATA 70 0.0067437207 28.38048 2 AGTACTC 110 1.01285994E-4 25.649351 5 TAGGTAG 100 0.0017510757 23.511906 5 GTGCATG 150 2.125794E-5 23.201921 1 ACCCAAG 110 0.0022041127 22.599274 1 CTGGTCG 105 0.0023467578 22.357124 9 CGGGTCG 110 0.0030463745 21.37446 4 TACTGGA 120 0.0036743348 20.6941 2 AGCGGTC 140 5.1828416E-4 20.153063 94 TAGGACC 140 5.1828416E-4 20.153063 4 GCCGTTC 190 9.095078E-6 19.768402 8 ACACACG 60 0.0051061017 19.554106 34-35 CATGGGG 965 0.0 19.491734 4 CAAGACA 145 6.5649033E-4 19.45813 4 CCTCAGA 180 8.665481E-5 19.334934 1 GGCCGTT 170 8.676292E-5 19.332335 7 GTCCAGA 130 0.005802861 19.122461 1 >>END_MODULE