FastQCFastQC Report
Wed 25 May 2016
SRR1295068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888265
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26550.025.4478241
GTACATG46300.024.6334671
ATCAACG24900.024.341073
TCAACGC25100.023.9235344
CAACGCA24800.023.1855165
TATCAAC29850.021.8005542
AACGCAG26150.021.6127936
TACATGG50850.021.2070772
CGCAGAG28500.019.4964878
ACATGGG55350.019.4669913
ACGCAGA29400.018.6125327
GTATATA14600.017.8313881
GTCTAGA9300.016.6627161
GGTATCA20950.016.2729911
CATGGGG37550.015.8420424
ACAGCGT3552.8763134E-715.5110998
GTATATG13700.015.3832131
TAGTGCG1950.003282463915.306537
GTATAAT11500.015.09207251
TTATACT15250.015.0879174
Produced by FastQC (version 0.11.5)