##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295068_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2888265
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.273737001279315	27.0	27.0	33.0	15.0	33.0
2	22.544067459183974	27.0	6.0	33.0	2.0	33.0
3	24.79394688506768	27.0	15.0	33.0	15.0	33.0
4	25.044372659710934	33.0	6.0	37.0	2.0	37.0
5	26.69384734433994	33.0	15.0	37.0	2.0	37.0
6	27.61836604328204	33.0	15.0	37.0	6.0	37.0
7	27.461762338289596	33.0	15.0	37.0	6.0	37.0
8	28.418566855880606	33.0	22.0	37.0	6.0	37.0
9	29.32585202535086	33.0	27.0	37.0	15.0	37.0
10-11	28.849550854925013	33.0	24.5	37.0	10.5	37.0
12-13	27.637014782230857	33.0	15.0	37.0	4.0	37.0
14-15	29.393088930551734	33.0	24.5	37.0	10.5	40.0
16-17	29.827720101860457	33.0	27.0	37.0	6.0	40.0
18-19	29.95456147548788	33.0	27.0	40.0	10.5	40.0
20-21	29.455762542564482	33.0	24.5	38.5	6.0	40.0
22-23	30.182052893346004	33.0	27.0	37.0	15.0	40.0
24-25	30.304406105395454	33.0	27.0	38.5	15.0	40.0
26-27	28.859744691016928	33.0	22.0	37.0	4.0	40.0
28-29	29.589881122403934	33.0	22.0	37.0	10.5	40.0
30-31	30.268953160461386	33.0	27.0	38.5	15.0	40.0
32-33	29.070295488814217	33.0	22.0	37.0	6.0	40.0
34-35	28.636034262784058	33.0	22.0	37.0	6.0	40.0
36-37	28.96967660515915	33.0	22.0	37.0	6.0	40.0
38-39	29.068339470235593	33.0	24.5	37.0	6.0	40.0
40-41	28.117671681788202	33.0	22.0	37.0	6.0	40.0
42-43	28.776268278707114	33.0	22.0	37.0	6.0	40.0
44-45	28.62720075893313	33.0	22.0	37.0	6.0	40.0
46-47	28.321833003550573	33.0	22.0	37.0	6.0	40.0
48-49	28.200568334276806	33.0	22.0	37.0	6.0	40.0
50-51	28.17893025743829	33.0	22.0	37.0	6.0	40.0
52-53	28.385903821152148	33.0	22.0	37.0	6.0	40.0
54-55	28.338944660548805	33.0	22.0	37.0	6.0	40.0
56-57	28.289221903114846	33.0	22.0	37.0	6.0	40.0
58-59	28.171290030520055	33.0	22.0	37.0	6.0	40.0
60-61	28.147042947928945	33.0	22.0	37.0	6.0	40.0
62-63	28.069309083480913	33.0	22.0	37.0	6.0	40.0
64-65	27.60659946369187	33.0	22.0	37.0	6.0	40.0
66-67	27.81583943994059	33.0	22.0	37.0	10.5	37.0
68-69	27.446961584203663	33.0	22.0	37.0	6.0	37.0
70-71	27.56144675090409	33.0	22.0	37.0	10.5	37.0
72-73	27.565200388468508	33.0	22.0	37.0	10.5	37.0
74-75	27.658944556680222	33.0	22.0	37.0	15.0	37.0
76-77	27.40192987831795	30.0	22.0	37.0	10.5	37.0
78-79	26.99918515094702	27.0	22.0	33.0	6.0	37.0
80-81	26.80625565867398	27.0	22.0	33.0	6.0	37.0
82-83	26.891092749453392	27.0	22.0	33.0	15.0	37.0
84-85	26.858645588268388	27.0	22.0	33.0	10.5	37.0
86-87	26.63257024545878	27.0	22.0	33.0	10.5	37.0
88-89	26.577713263845247	27.0	22.0	33.0	10.5	37.0
90-91	26.717353843916676	27.0	22.0	33.0	15.0	37.0
92-93	26.654672787988638	27.0	22.0	33.0	15.0	37.0
94-95	26.544640294432817	27.0	22.0	33.0	15.0	37.0
96-97	26.618417458231846	27.0	22.0	33.0	15.0	37.0
98-99	26.691593569149646	27.0	22.0	33.0	15.0	37.0
100	26.361832795813402	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	817.0
3	705.0
4	3183.0
5	12972.0
6	20484.0
7	23057.0
8	21405.0
9	21810.0
10	26096.0
11	32330.0
12	41070.0
13	54342.0
14	44200.0
15	49084.0
16	55963.0
17	59865.0
18	60157.0
19	60515.0
20	60032.0
21	58830.0
22	61801.0
23	67530.0
24	74024.0
25	81812.0
26	89648.0
27	96541.0
28	103494.0
29	108994.0
30	115433.0
31	125042.0
32	143424.0
33	171713.0
34	204483.0
35	235351.0
36	247531.0
37	199014.0
38	55423.0
39	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.362696268308454	20.477560935780627	13.650408885286646	24.509333910624274
2	15.329246241634738	21.074192329889215	39.9062157878322	23.69034564064385
3	18.046726296248792	26.175737593576265	29.91537019429904	25.862165915875906
4	12.253423436460194	17.264272011402458	39.01472348722145	31.467581064915894
5	13.044339868063224	37.55804456290762	35.88481369091771	13.512801878111452
6	30.582773086244046	37.94055938836062	18.77296987546648	12.703697649928857
7	26.202660054102793	31.61861286444244	24.671626089570182	17.50710099188458
8	23.88981493112019	36.65090320237097	21.796016471858312	17.663265394650534
9	24.761878720929808	16.746362466413654	21.90289216276026	36.58886664989628
10-11	23.255746275808264	26.995610136852587	29.632326949993228	20.11631663734592
12-13	24.188216673398895	24.680145569543814	29.258209183273422	21.87342857378387
14-15	21.481182436120662	26.299579447278887	26.51820202999498	25.701036086605466
16-17	21.2993412891439	29.69625226309562	27.104568920659037	21.899837527101443
18-19	21.15374868058919	28.90925746352729	28.664909795402068	21.272084060481458
20-21	22.68557266987954	28.355311215207003	27.580029338841115	21.379086776072345
22-23	21.86946917655717	28.28292453725711	28.11638364366113	21.73122264252459
24-25	21.943331545847908	28.214068671166558	27.80527482634011	22.037324956645428
26-27	21.77507917000923	28.211270169138185	27.891664990605136	22.121985670247447
28-29	21.699871426849057	28.164938092534015	28.15608430790923	21.979106172707702
30-31	22.18187089247261	28.51992995259471	27.555072254212654	21.743126900720025
32-33	21.52999156417884	28.36062615898075	28.066175380060663	22.043206896779747
34-35	21.761979989664137	27.86561069897719	27.931265949731674	22.441143361627
36-37	22.089290022684597	28.48267621576271	27.496560092330807	21.931473669221884
38-39	21.800783837491718	28.254397585927727	27.815779789504674	22.12903878707588
40-41	22.056339522887022	28.087637327516223	27.565019016576457	22.291004133020294
42-43	21.721351368690716	28.209128242717778	28.04296879896938	22.026551589622123
44-45	22.042346045535734	27.885651617916952	27.984840486918454	22.087161849628863
46-47	21.982529446643543	28.262432074599324	27.895131929656348	21.859906549100785
48-49	21.863787800551062	28.035378333524413	27.96745383374768	22.13338003217685
50-51	22.180381756777955	27.985821685683398	27.72687498777509	22.106921569763557
52-53	21.824898370166622	27.99174176343291	27.93440794662953	22.24895191977094
54-55	22.18128222685161	28.054792231591986	27.75778302872015	22.006142512836256
56-57	22.14906542489423	28.018596765386217	27.679308352779213	22.153029456940338
58-59	21.849868424563923	27.60460350150238	28.11408780423853	22.43144026969517
60-61	22.20105253697936	27.996723631510122	27.648285300520183	22.153938530990334
62-63	21.91397015017011	28.10309260686444	27.865731094518747	22.117206148446698
64-65	21.993731391029435	27.594957941484893	27.894295522362643	22.517015145123025
66-67	21.89203085015273	27.855995258297405	27.683182757909442	22.568791133640424
68-69	22.333499385799733	27.764214706020795	27.6856108843666	22.21667502381287
70-71	22.41577209240041	27.764424013677402	27.545719470106896	22.274084423815292
72-73	22.036025749095508	27.964158760433357	27.851941822212595	22.147873668258544
74-75	22.14982673631285	27.666165082522426	27.61157049443336	22.57243768673137
76-77	22.247196088896303	27.93469839881354	27.498183157038113	22.319922355252046
78-79	22.25520541201992	27.725919255109847	27.70228329049938	22.316592042370857
80-81	22.39861114913104	27.64411932743872	27.57672544498771	22.380544078442526
82-83	21.991091355985063	27.805361232991494	28.018554035471322	22.184993375552125
84-85	22.1718950694625	27.506713351492433	27.650112465601463	22.671279113443603
86-87	22.24511660974575	27.79987680519443	27.638255379248477	22.316751205811343
88-89	22.071022389327524	27.42062081449416	27.920692502482915	22.5876642936954
90-91	22.250137112565767	27.4084369584575	27.67586728476803	22.665558644208705
92-93	22.0455418808384	27.695801666491786	27.831774804717767	22.426881647952047
94-95	22.06306591071864	27.220513234454234	27.801199888594446	22.91522096623267
96-97	22.077029878900262	27.430340815801436	27.82213050714506	22.670498798153243
98-99	22.034656858432392	27.37669955513115	27.862410929731723	22.726232656704727
100	22.30691587120722	27.087220197922274	27.561574195810323	23.04428973506018
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	329.0
1	407.5
2	402.0
3	299.0
4	305.0
5	402.5
6	597.0
7	849.5
8	1182.0
9	1700.5
10	2458.0
11	3286.5
12	4166.5
13	5178.5
14	6107.5
15	6908.0
16	7738.5
17	8574.0
18	9392.0
19	10115.5
20	10756.5
21	11600.0
22	12694.0
23	13836.5
24	15348.0
25	17537.5
26	20640.0
27	24946.5
28	30685.5
29	37704.0
30	45828.5
31	54575.0
32	63515.5
33	72627.0
34	81125.0
35	88879.0
36	95879.5
37	102130.5
38	107376.5
39	111106.0
40	112847.0
41	113352.0
42	112533.0
43	110137.5
44	106770.0
45	102826.0
46	98501.0
47	94066.5
48	89812.0
49	85989.5
50	82658.0
51	78673.0
52	73929.5
53	68749.5
54	63587.5
55	58866.0
56	54307.0
57	49167.5
58	43619.0
59	37952.0
60	32582.0
61	27537.5
62	22414.5
63	17754.0
64	14052.0
65	11212.0
66	8706.0
67	6623.0
68	5126.5
69	3916.5
70	2943.0
71	2252.0
72	1702.5
73	1270.5
74	914.0
75	603.5
76	413.0
77	276.0
78	188.5
79	128.5
80	82.0
81	60.5
82	43.5
83	26.0
84	16.5
85	13.0
86	8.0
87	7.0
88	6.5
89	4.0
90	3.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	1.0
97	1.0
98	1.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.558967061540406
2	24.289426351113903
3	5.794343663064158
4	23.67608235393913
5	12.293955021440208
6	9.55161662797562
7	7.84779097485861
8	8.212473578428572
9	5.031913622884327
10-11	6.60605934704745
12-13	9.700096770898792
14-15	7.077466922183387
16-17	7.546866371333655
18-19	6.417485930134527
20-21	7.668721533515796
22-23	5.532941056308891
24-25	5.056720903379711
26-27	9.452283637408618
28-29	5.9521027329555976
30-31	4.10026434555001
32-33	8.372240774305682
34-35	6.9766278371271335
36-37	6.508215139538789
38-39	5.9158352851971685
40-41	8.324842076471516
42-43	6.0974668183148015
44-45	6.1726849856228565
46-47	5.609613383813466
48-49	3.8147642269667084
50-51	6.184820298691429
52-53	4.133727341500867
54-55	4.685165661738102
56-57	4.796633965373676
58-59	3.3418159344796963
60-61	3.878470292718985
62-63	3.0067185663365374
64-65	4.431847493218247
66-67	2.3898257258250193
68-69	4.747711861619345
70-71	2.268039809366523
72-73	1.4847494949390032
74-75	0.7506409557294778
76-77	1.1676733263741381
78-79	1.6361206468243044
80-81	1.9786099959664367
82-83	0.7482346668328564
84-85	1.140736739876708
86-87	0.48317934815538044
88-89	0.7504851528512793
90-91	0.38317121178285235
92-93	0.3504872302229885
94-95	0.48754182874493857
96-97	0.4897923147633614
98-99	0.43576333889030266
100	0.4569179074634772
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2888265.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.59728013876433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.40422154181374	91.19577151750453
2	2.78803911071415	5.274818335881156
3	0.46488244798763984	1.3192984549164388
4	0.1335956845424723	0.5055115358397697
5	0.0733202780818091	0.34679494427784985
6	0.03825157287891936	0.21710968532252967
7	0.0225123358790541	0.1490724020610185
8	0.018254449679660743	0.13814570321006753
9	0.011672226477765259	0.09937447901642363
>10	0.04442441510600875	0.6563317152030257
>50	4.930056640830809E-4	0.030338962781326383
>100	3.329311747290821E-4	0.0674322639859254
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.462286182188961E-5	0.0
4	0.0	3.462286182188961E-5	0.0	3.462286182188961E-5	0.0
5	0.0	3.462286182188961E-5	0.0	3.462286182188961E-5	0.0
6	0.0	3.462286182188961E-5	0.0	3.462286182188961E-5	0.0
7	0.0	3.462286182188961E-5	0.0	3.462286182188961E-5	0.0
8	0.0	3.462286182188961E-5	0.0	3.462286182188961E-5	0.0
9	0.0	6.924572364377922E-5	0.0	6.924572364377922E-5	0.0
10-11	0.0	6.924572364377922E-5	0.0	6.924572364377922E-5	0.0
12-13	0.0	6.924572364377922E-5	0.0	6.924572364377922E-5	0.0
14-15	0.0	6.924572364377922E-5	0.0	1.0386858546566884E-4	0.0
16-17	3.462286182188961E-5	6.924572364377922E-5	0.0	1.0386858546566884E-4	0.0
18-19	3.462286182188961E-5	6.924572364377922E-5	0.0	1.2118001637661364E-4	0.0
20-21	3.462286182188961E-5	6.924572364377922E-5	0.0	1.3849144728755844E-4	0.0
22-23	3.462286182188961E-5	1.0386858546566884E-4	0.0	1.3849144728755844E-4	0.0
24-25	8.655715455472403E-5	1.2118001637661364E-4	0.0	2.4236003275322727E-4	0.0
26-27	1.0386858546566884E-4	1.3849144728755844E-4	0.0	3.2891718730795134E-4	0.0
28-29	1.0386858546566884E-4	1.5580287819850325E-4	0.0	5.885886509721233E-4	0.0
30-31	1.0386858546566884E-4	1.7311430910944806E-4	0.0	0.002181240294779046	0.0
32-33	1.0386858546566884E-4	1.7311430910944806E-4	0.0	0.006820703778912253	0.0
34-35	1.0386858546566884E-4	1.7311430910944806E-4	0.0	0.014091504761509072	0.0
36-37	1.0386858546566884E-4	1.9042574002039287E-4	0.0	0.025811343488218706	0.0
38-39	1.0386858546566884E-4	2.0773717093133768E-4	0.0	0.04440382028657343	0.0
40-41	1.0386858546566884E-4	2.0773717093133768E-4	0.0	0.06687405760897978	0.0
42-43	1.0386858546566884E-4	2.250486018422825E-4	0.0	0.0908330779897274	0.0
44-45	1.0386858546566884E-4	2.4236003275322727E-4	0.0	0.11924113611458782	0.0
46-47	1.0386858546566884E-4	2.4236003275322727E-4	0.0	0.15327540928550532	0.0
48-49	1.0386858546566884E-4	2.4236003275322727E-4	0.0	0.1907546572077008	3.462286182188961E-5
50-51	1.0386858546566884E-4	2.4236003275322727E-4	0.0	0.2294630167245734	3.462286182188961E-5
52-53	1.0386858546566884E-4	2.596714636641721E-4	0.0	0.2724646111073603	3.462286182188961E-5
54-55	1.0386858546566884E-4	2.769828945751169E-4	0.0	0.3234294637091818	3.462286182188961E-5
56-57	1.2118001637661364E-4	2.769828945751169E-4	0.0	0.38171705158633296	3.462286182188961E-5
58-59	1.3849144728755844E-4	2.769828945751169E-4	0.0	0.44557892021680834	3.462286182188961E-5
60-61	1.7311430910944806E-4	2.769828945751169E-4	0.0	0.5169885727244556	3.462286182188961E-5
62-63	2.0773717093133768E-4	2.9429432548606167E-4	0.0	0.5917566428288263	5.1934292732834413E-5
64-65	2.0773717093133768E-4	3.116057563970065E-4	0.0	0.6772058658052499	6.924572364377922E-5
66-67	2.0773717093133768E-4	3.116057563970065E-4	0.0	0.777214002177778	6.924572364377922E-5
68-69	2.0773717093133768E-4	3.116057563970065E-4	0.0	0.8871588998931885	6.924572364377922E-5
70-71	2.0773717093133768E-4	3.116057563970065E-4	0.0	1.0164406659361243	6.924572364377922E-5
72-73	2.0773717093133768E-4	3.116057563970065E-4	0.0	1.1528028072216365	6.924572364377922E-5
74-75	2.0773717093133768E-4	3.116057563970065E-4	0.0	1.3030833389595484	6.924572364377922E-5
76-77	2.0773717093133768E-4	3.116057563970065E-4	0.0	1.4713504474139318	6.924572364377922E-5
78-79	2.0773717093133768E-4	3.116057563970065E-4	0.0	1.6594564556922582	6.924572364377922E-5
80-81	2.0773717093133768E-4	3.116057563970065E-4	0.0	1.868422738218273	6.924572364377922E-5
82-83	2.0773717093133768E-4	3.116057563970065E-4	0.0	2.0972279205682307	6.924572364377922E-5
84-85	2.0773717093133768E-4	3.116057563970065E-4	0.0	2.3326806923879904	6.924572364377922E-5
86-87	2.0773717093133768E-4	3.116057563970065E-4	0.0	2.599207482692897	6.924572364377922E-5
88	2.0773717093133768E-4	3.116057563970065E-4	0.0	2.8246023131533984	6.924572364377922E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2655	0.0	25.447824	1
GTACATG	4630	0.0	24.633467	1
ATCAACG	2490	0.0	24.34107	3
TCAACGC	2510	0.0	23.923534	4
CAACGCA	2480	0.0	23.185516	5
TATCAAC	2985	0.0	21.800554	2
AACGCAG	2615	0.0	21.612793	6
TACATGG	5085	0.0	21.207077	2
CGCAGAG	2850	0.0	19.496487	8
ACATGGG	5535	0.0	19.466991	3
ACGCAGA	2940	0.0	18.612532	7
GTATATA	1460	0.0	17.831388	1
GTCTAGA	930	0.0	16.662716	1
GGTATCA	2095	0.0	16.272991	1
CATGGGG	3755	0.0	15.842042	4
ACAGCGT	355	2.8763134E-7	15.511099	8
GTATATG	1370	0.0	15.383213	1
TAGTGCG	195	0.0032824639	15.30653	7
GTATAAT	1150	0.0	15.0920725	1
TTATACT	1525	0.0	15.087917	4
>>END_MODULE