FastQCFastQC Report
Wed 25 May 2016
SRR1295068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888265
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39950.032.9889451
ATCAACG42150.029.6748183
TCAACGC42500.029.4314584
CAACGCA42850.028.9715795
AACGCAG45050.027.5204856
TATCAAC48150.026.5258922
GTACATG63400.024.929011
GGTATCA27100.024.498481
ACGCAGA51800.023.7497087
CGCAGAG52250.023.365438
TACATGG68250.023.2110022
ACATGGG71650.021.1321493
CATGGGG44800.018.5786574
GCAGAGT66150.018.3140379
AGAGTAC55550.016.48346710-11
GAGTACT26750.015.09624412-13
GTATTAT15100.015.093311
TATACTG14900.014.8330565
GTACATA14800.014.7297231
CTATACT13500.014.6296724
Produced by FastQC (version 0.11.5)