##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295068_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2888265
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43713440421845	33.0	33.0	33.0	27.0	33.0
2	30.231552852664144	33.0	33.0	33.0	27.0	33.0
3	30.993869329857198	33.0	33.0	33.0	27.0	33.0
4	35.23789679963577	37.0	37.0	37.0	33.0	37.0
5	35.53589300150782	37.0	37.0	37.0	33.0	37.0
6	35.28875085907976	37.0	37.0	37.0	33.0	37.0
7	35.19769792591746	37.0	37.0	37.0	33.0	37.0
8	35.17883400588242	37.0	37.0	37.0	33.0	37.0
9	35.172286476483286	37.0	37.0	37.0	33.0	37.0
10-11	35.13641459492118	37.0	37.0	37.0	33.0	37.0
12-13	35.05524008357959	37.0	37.0	37.0	33.0	37.0
14-15	36.527838165819276	40.0	37.0	40.0	33.0	40.0
16-17	36.51302892913219	40.0	37.0	40.0	33.0	40.0
18-19	36.53322929163356	40.0	37.0	40.0	33.0	40.0
20-21	36.53382393928535	40.0	37.0	40.0	33.0	40.0
22-23	36.48940557739681	40.0	37.0	40.0	33.0	40.0
24-25	36.45526951301214	40.0	37.0	40.0	33.0	40.0
26-27	36.44114044244555	40.0	37.0	40.0	33.0	40.0
28-29	36.388985082739985	38.5	37.0	40.0	33.0	40.0
30-31	36.31816419199762	37.0	37.0	40.0	33.0	40.0
32-33	36.27360647309024	37.0	37.0	40.0	30.0	40.0
34-35	36.17937671924148	37.0	37.0	40.0	27.0	40.0
36-37	36.17575170560873	37.0	37.0	40.0	27.0	40.0
38-39	36.11361042009649	37.0	37.0	40.0	27.0	40.0
40-41	36.05898298805684	37.0	37.0	40.0	27.0	40.0
42-43	35.979235284850944	37.0	37.0	40.0	27.0	40.0
44-45	35.88157509785286	37.0	37.0	40.0	27.0	40.0
46-47	35.79090647845679	37.0	37.0	40.0	27.0	40.0
48-49	35.69224326715174	37.0	37.0	40.0	27.0	40.0
50-51	35.594870969249705	37.0	37.0	40.0	27.0	40.0
52-53	35.48947707360647	37.0	37.0	40.0	27.0	40.0
54-55	35.3639269596107	37.0	33.0	40.0	27.0	40.0
56-57	35.24062888966213	37.0	33.0	40.0	27.0	40.0
58-59	35.10359904648639	37.0	33.0	40.0	27.0	40.0
60-61	34.94435067419367	37.0	33.0	40.0	27.0	40.0
62-63	34.75838557057611	37.0	33.0	40.0	27.0	40.0
64-65	34.56818799521512	37.0	33.0	40.0	27.0	40.0
66-67	34.337014436002235	37.0	33.0	40.0	27.0	40.0
68-69	34.11642872104879	37.0	33.0	37.0	27.0	40.0
70-71	33.89137180971967	37.0	33.0	37.0	27.0	40.0
72-73	33.68046422333131	37.0	33.0	37.0	27.0	40.0
74-75	33.44108089112322	37.0	33.0	37.0	27.0	40.0
76-77	33.2093365740332	37.0	33.0	37.0	24.5	37.0
78-79	32.98371254022744	37.0	33.0	37.0	22.0	37.0
80-81	32.76973581025287	37.0	33.0	37.0	22.0	37.0
82-83	32.55901380240387	37.0	33.0	37.0	22.0	37.0
84-85	32.39297069347861	33.0	33.0	37.0	22.0	37.0
86-87	32.43022125739847	33.0	33.0	37.0	22.0	37.0
88-89	32.4456261804232	33.0	33.0	37.0	22.0	37.0
90-91	32.467398593965584	33.0	33.0	37.0	22.0	37.0
92-93	32.4502784543662	33.0	33.0	37.0	22.0	37.0
94-95	32.485140040820355	33.0	33.0	37.0	22.0	37.0
96-97	32.44791838699011	33.0	33.0	37.0	22.0	37.0
98-99	32.376611564382074	33.0	33.0	37.0	22.0	37.0
100	31.848392373968455	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	12.0
4	30.0
5	56.0
6	47.0
7	41.0
8	55.0
9	69.0
10	416.0
11	1696.0
12	4888.0
13	8925.0
14	11797.0
15	13499.0
16	14784.0
17	15203.0
18	15762.0
19	16583.0
20	18448.0
21	20256.0
22	22213.0
23	23851.0
24	25465.0
25	27158.0
26	30469.0
27	34863.0
28	41405.0
29	49219.0
30	60373.0
31	74408.0
32	95324.0
33	127580.0
34	176781.0
35	252925.0
36	389257.0
37	670716.0
38	640439.0
39	3249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.756970339588605	20.208425266178264	10.031258910079046	31.00334548415409
2	13.296273479847336	22.5965348429452	36.23790238799115	27.869289289216308
3	15.392701154499328	26.64842041848653	28.909674146935966	29.04920428007818
4	10.726385888543064	18.197128693570463	37.2260173062294	33.850468111657065
5	11.704992828150274	38.647183835177856	35.295280451230035	14.352542885441839
6	30.318870684225736	39.53294611375055	17.445112905941393	12.703070296082327
7	26.525725005126162	32.986867335006956	23.007655987630717	17.47975167223617
8	24.697043015129058	36.82914317166044	20.599169853697365	17.874643959513133
9	25.062711680063398	16.447363407691387	21.352591009005124	37.13733390324009
10-11	23.369321618118448	27.259904716313848	29.249022673693208	20.121750991874492
12-13	24.11037963739748	24.762436534860537	29.30840749867164	21.818776329070342
14-15	21.516994297420208	26.40215120582735	26.274974982152376	25.805879514600065
16-17	21.1658497007922	29.78338065414419	26.91463983797493	22.136129807088682
18-19	21.015692494232265	28.9315870561036	28.90059030626001	21.152130143404126
20-21	22.4473911455708	28.234109726878447	28.020538444129567	21.29796068342118
22-23	21.74952208785272	28.134497638144317	28.323065148429816	21.79291512557315
24-25	21.75878766156129	27.98637642990779	28.263713392499245	21.991122516031677
26-27	21.513572286841168	28.10903884177612	28.318760886050963	22.05862798533175
28-29	21.36906713966673	28.103984981647816	28.556720702213934	21.970227176471525
30-31	21.964380457112618	28.416673708474686	27.94820877183018	21.67073706258251
32-33	21.296240834756066	28.314609581469007	28.58344288750757	21.805706696267357
34-35	21.49699262555743	27.843666734126217	28.579033537206595	22.08030710310976
36-37	21.77061913050343	28.344801534279433	28.02793571344236	21.856643621774772
38-39	21.593329514167873	28.394768054948894	28.268801382794123	21.743101048089112
40-41	21.802139570700845	28.0363157778104	28.146753387053437	22.014791264435317
42-43	21.409749989958602	28.147510910498593	28.358120582650404	22.0846185168924
44-45	21.697552986312374	27.894726818812956	28.310705295341776	22.097014899532898
46-47	21.76051752142531	28.313025525454034	28.01552896713011	21.910927985990547
48-49	21.58409405509751	28.17701408727761	28.201949160848194	22.03694269677669
50-51	21.901050117000967	28.047540105340694	27.896029308941078	22.15538046871726
52-53	21.656077316255534	28.149518591639193	28.034831173297487	22.159572918807786
54-55	21.912654970492902	28.14572546632056	27.915614944352875	22.02600461883367
56-57	21.878871375829508	28.02556218328845	27.90366930462017	22.19189713626187
58-59	21.5006723066785	27.73362873504191	28.388742507754124	22.376956450525466
60-61	22.003489390980597	28.04982796301737	27.786937655141035	22.159744990860993
62-63	21.594693531681212	28.276242753368486	27.97868976954491	22.15037394540539
64-65	21.59851866268236	27.72351048132035	28.114899913805676	22.563070942191622
66-67	21.598845559867623	27.965235200381212	27.926397169169842	22.509522070581326
68-69	21.796213157840967	27.924731395062512	28.01463867904173	22.264416768054794
70-71	21.898761489066878	27.812468505610028	27.829230880589208	22.459539124733887
72-73	21.540545566815087	28.096046827581645	28.041778974341035	22.32162863126223
74-75	21.77858077796935	27.724199217698185	27.91429415954157	22.582925844790893
76-77	21.719137825205028	28.023485839496693	27.767659181752936	22.489717153545342
78-79	21.67960559554567	27.977070410420126	28.01093705686376	22.332386937170444
80-81	21.90790775643302	27.76495491722315	27.805227029270345	22.521910297073486
82-83	21.475581206516203	27.993705680849835	28.13217516198921	22.398537950644755
84-85	21.626395468018504	27.614061331338817	27.969819108562564	22.789724092080114
86-87	21.64110123281607	27.937990600009037	27.8677166041403	22.5531915630346
88-89	21.42148679060657	27.625678249899284	28.128129826479174	22.824705133014973
90-91	21.580551532742334	27.64211399243605	27.941313190508797	22.836021284312817
92-93	21.337440017561494	27.963891602095053	28.010998093234495	22.687670287108958
94-95	21.333177758823386	27.4112303445081	28.167267065401365	23.08832483126715
96-97	21.325213729072	27.689842519794087	28.03140048112412	22.953543270009792
98-99	21.2138549904622	27.65174485747311	28.12391748690251	23.010482665162176
100	21.49542428563605	27.42458095523973	27.864509496314405	23.215485262809814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	9.5
2	12.0
3	12.5
4	15.0
5	30.5
6	61.0
7	110.5
8	171.0
9	198.0
10	167.0
11	143.0
12	173.5
13	215.0
14	235.0
15	285.5
16	344.0
17	400.5
18	512.0
19	675.0
20	885.5
21	1225.0
22	1723.0
23	2464.0
24	3717.0
25	5648.5
26	8660.5
27	13214.5
28	19466.5
29	27557.5
30	37078.5
31	47361.5
32	57807.5
33	68343.0
34	78889.5
35	88584.0
36	97423.0
37	105768.0
38	112673.0
39	117470.0
40	120817.5
41	122320.0
42	122277.5
43	120459.5
44	116647.0
45	112374.5
46	107894.0
47	103096.0
48	98896.5
49	95546.5
50	93096.5
51	89726.5
52	83726.5
53	77575.0
54	72206.0
55	67239.0
56	62522.5
57	56681.0
58	49793.5
59	43615.0
60	37404.0
61	30836.5
62	24804.0
63	19331.5
64	15107.5
65	11855.5
66	8994.5
67	6702.0
68	4891.5
69	3568.0
70	2640.5
71	1927.0
72	1419.0
73	984.0
74	668.0
75	412.0
76	212.0
77	112.5
78	61.5
79	37.0
80	22.0
81	10.0
82	6.5
83	5.0
84	2.5
85	0.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.669017558984373
2	5.018756935392009
3	0.0
4	0.005955132233365013
5	0.16511642802859156
6	0.058962733682678015
7	0.03853524520776314
8	0.045148211815744056
9	0.04303621724460879
10-11	0.045252080401209725
12-13	0.043174708691896345
14-15	0.04590991477582562
16-17	0.036613676376648266
18-19	0.029966086906845463
20-21	0.024391806153521232
22-23	0.024409117584432177
24-25	0.021552731484126283
26-27	0.013676030419646396
28-29	0.020704471369489986
30-31	0.025932523504595323
32-33	0.001523405920163143
34-35	0.0039643176786063605
36-37	0.004639463484133208
38-39	0.0069072609334669775
40-41	0.007236178120774929
42-43	0.0076516524626376045
44-45	0.00882882976458185
46-47	0.01732874234185575
48-49	0.026521112155567444
50-51	0.023612791762528715
52-53	0.029481366841339007
54-55	0.025603606317287367
56-57	0.01674015369088363
58-59	0.015441796372562766
60-61	0.012758524581366323
62-63	0.01883483683110795
64-65	0.020912208540421324
66-67	0.02186433724052329
68-69	0.02929094110131861
70-71	0.02929094110131861
72-73	0.025482426300910757
74-75	0.026330686415547047
76-77	0.019648474083922354
78-79	0.01620349933264434
80-81	0.014212684777885686
82-83	0.009140435520978858
84-85	0.011944887328551916
86-87	0.009815581326505705
88-89	0.007807455340836107
90-91	0.007703586755370438
92-93	0.00443172631320187
94-95	0.0015753402128959773
96-97	0.0010040629928347988
98-99	7.09768667348737E-4
100	0.11255892378296312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2888265.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.46994008670377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.73867645295303	69.79856097369114
2	9.42656581224061	15.171103722687441
3	2.1345715258353763	5.153065283842698
4	0.7392498027742347	2.37949549353401
5	0.31945373214879513	1.2853211343243731
6	0.17302104322323258	0.8353795789147505
7	0.11370929757461028	0.6405126254191043
8	0.06725806866995046	0.43298022049746576
9	0.04856341405530337	0.35171055174924454
>10	0.22792058135475862	3.112816549084052
>50	0.008963572174687674	0.47891795727119196
>100	0.0018741152091046479	0.23226540833317733
>500	8.629089319932237E-5	0.05388406652483429
>1k	8.629089319926655E-5	0.07398643412651273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.462286182188961E-5	0.0
3	0.0	0.0	0.0	3.462286182188961E-5	0.0
4	0.0	0.0	0.0	3.462286182188961E-5	0.0
5	0.0	0.0	0.0	3.462286182188961E-5	0.0
6	0.0	0.0	0.0	3.462286182188961E-5	0.0
7	0.0	0.0	0.0	3.462286182188961E-5	0.0
8	0.0	0.0	0.0	3.462286182188961E-5	0.0
9	0.0	3.462286182188961E-5	0.0	3.462286182188961E-5	0.0
10-11	0.0	3.462286182188961E-5	0.0	5.1934292732834413E-5	0.0
12-13	0.0	3.462286182188961E-5	0.0	6.924572364377922E-5	0.0
14-15	0.0	3.462286182188961E-5	0.0	6.924572364377922E-5	0.0
16-17	0.0	3.462286182188961E-5	0.0	6.924572364377922E-5	0.0
18-19	5.1934292732834413E-5	6.924572364377922E-5	0.0	6.924572364377922E-5	0.0
20-21	6.924572364377922E-5	6.924572364377922E-5	0.0	1.2118001637661364E-4	0.0
22-23	6.924572364377922E-5	6.924572364377922E-5	0.0	1.3849144728755844E-4	0.0
24-25	6.924572364377922E-5	6.924572364377922E-5	0.0	2.769828945751169E-4	0.0
26-27	6.924572364377922E-5	6.924572364377922E-5	0.0	3.2891718730795134E-4	0.0
28-29	6.924572364377922E-5	6.924572364377922E-5	0.0	8.828829764581851E-4	0.0
30-31	6.924572364377922E-5	6.924572364377922E-5	0.0	0.003462286182188961	0.0
32-33	6.924572364377922E-5	6.924572364377922E-5	0.0	0.010767710026607669	0.0
34-35	8.655715455472403E-5	8.655715455472403E-5	0.0	0.02103338855679794	0.0
36-37	1.0386858546566884E-4	1.3849144728755844E-4	0.0	0.03912383385873526	0.0
38-39	1.0386858546566884E-4	1.7311430910944806E-4	0.0	0.06716835193446585	0.0
40-41	1.3849144728755844E-4	1.7311430910944806E-4	0.0	0.10028511926710326	0.0
42-43	1.3849144728755844E-4	1.7311430910944806E-4	0.0	0.13556581546360877	0.0
44-45	1.3849144728755844E-4	1.7311430910944806E-4	0.0	0.17763259257720465	0.0
46-47	1.3849144728755844E-4	1.9042574002039287E-4	0.0	0.22793961080441028	1.7311430910944806E-5
48-49	1.3849144728755844E-4	2.0773717093133768E-4	0.0	0.2821936352793113	3.462286182188961E-5
50-51	1.3849144728755844E-4	2.0773717093133768E-4	0.0	0.33942522587089485	3.462286182188961E-5
52-53	1.3849144728755844E-4	2.0773717093133768E-4	0.0	0.40231765437035727	3.462286182188961E-5
54-55	1.3849144728755844E-4	2.0773717093133768E-4	0.0	0.4732772096743201	3.462286182188961E-5
56-57	1.3849144728755844E-4	2.250486018422825E-4	0.0	0.5566144380796083	3.462286182188961E-5
58-59	1.3849144728755844E-4	2.4236003275322727E-4	0.0	0.648313087614883	3.462286182188961E-5
60-61	1.3849144728755844E-4	2.4236003275322727E-4	0.0	0.7494291555657115	3.462286182188961E-5
62-63	1.3849144728755844E-4	2.4236003275322727E-4	0.0	0.8555482270498033	3.462286182188961E-5
64-65	1.3849144728755844E-4	2.4236003275322727E-4	0.0	0.9738718573261109	3.462286182188961E-5
66-67	1.3849144728755844E-4	2.4236003275322727E-4	0.0	1.1104244243516437	3.462286182188961E-5
68-69	1.3849144728755844E-4	2.4236003275322727E-4	0.0	1.260826136105932	3.462286182188961E-5
70-71	1.3849144728755844E-4	2.4236003275322727E-4	0.0	1.430201176138616	3.462286182188961E-5
72-73	1.7311430910944806E-4	2.4236003275322727E-4	0.0	1.6085089145213476	3.462286182188961E-5
74-75	1.7311430910944806E-4	2.4236003275322727E-4	0.0	1.8014448120238273	3.462286182188961E-5
76-77	1.7311430910944806E-4	2.4236003275322727E-4	0.0	2.0169548154341794	3.462286182188961E-5
78-79	2.0773717093133768E-4	2.4236003275322727E-4	0.0	2.2607343855221043	3.462286182188961E-5
80-81	2.250486018422825E-4	2.4236003275322727E-4	0.0	2.5265340957287505	6.924572364377922E-5
82-83	2.4236003275322727E-4	2.4236003275322727E-4	0.0	2.8104761855300673	6.924572364377922E-5
84-85	2.4236003275322727E-4	3.116057563970065E-4	0.0	3.107488405669147	6.924572364377922E-5
86-87	2.4236003275322727E-4	3.116057563970065E-4	0.0	3.434709072747826	6.924572364377922E-5
88	2.4236003275322727E-4	3.116057563970065E-4	0.0	3.7101858728336907	6.924572364377922E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3995	0.0	32.988945	1
ATCAACG	4215	0.0	29.674818	3
TCAACGC	4250	0.0	29.431458	4
CAACGCA	4285	0.0	28.971579	5
AACGCAG	4505	0.0	27.520485	6
TATCAAC	4815	0.0	26.525892	2
GTACATG	6340	0.0	24.92901	1
GGTATCA	2710	0.0	24.49848	1
ACGCAGA	5180	0.0	23.749708	7
CGCAGAG	5225	0.0	23.36543	8
TACATGG	6825	0.0	23.211002	2
ACATGGG	7165	0.0	21.132149	3
CATGGGG	4480	0.0	18.578657	4
GCAGAGT	6615	0.0	18.314037	9
AGAGTAC	5555	0.0	16.483467	10-11
GAGTACT	2675	0.0	15.096244	12-13
GTATTAT	1510	0.0	15.09331	1
TATACTG	1490	0.0	14.833056	5
GTACATA	1480	0.0	14.729723	1
CTATACT	1350	0.0	14.629672	4
>>END_MODULE