FastQCFastQC Report
Wed 25 May 2016
SRR1295067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595453
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC62800.17466505611393054No Hit
ATTCCATTCCATTCCATTCCATTCC62160.17288503006436184No Hit
GTATCAACGCAGAGTACTTTTTTTT56210.15633635038477767No Hit
CCCATGTACTCTGCGTTGATACCAC54790.15238691758729705No Hit
GGTATCAACGCAGAGTACTTTTTTT46340.12888501115158507No Hit
GAATGGAATGGAATGGAATGGAATG44940.12499120416815349No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN43070.1197901905545699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCGC951.3632645E-510.99868510
TCGGAAC909.547148E-510.55282313
ACGATTC1106.0689035E-610.36109718
CAGGACG750.002636964210.1389054
CGAATTG951.6492569E-49.9975519
ACGAATT1054.119354E-59.94994418
CTTACAC5400.09.8563063
GTCTTAG4500.09.7211951
ACTCGGA1606.668779E-89.49767311
GAACGAT1201.7070322E-59.497539516
GTGTTAG5650.09.4257081
TTAGACA5100.09.3188474
TATACTG10200.09.3185865
GGAACGA2451.8189894E-129.30345315
CTCGGAA1554.280082E-79.19129712
GGCGCCC1054.5004708E-49.0465378
GTCTAAG3050.09.0421671
CTAGGAC4000.09.029083
TCTGGAC3800.09.0040693
GTATTAG6150.08.9686551
Produced by FastQC (version 0.11.5)