##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295067_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3595453
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.342263130682003	33.0	33.0	33.0	27.0	33.0
2	31.363823418078333	33.0	33.0	33.0	27.0	33.0
3	31.350903766507308	33.0	33.0	33.0	27.0	33.0
4	35.050940173602605	37.0	37.0	37.0	33.0	37.0
5	35.18194397201132	37.0	37.0	37.0	33.0	37.0
6	35.225377720137075	37.0	37.0	37.0	33.0	37.0
7	35.1449436274094	37.0	37.0	37.0	33.0	37.0
8	34.99983145378343	37.0	37.0	37.0	33.0	37.0
9	34.981033822441844	37.0	37.0	37.0	33.0	37.0
10	34.90362188019145	37.0	37.0	37.0	33.0	37.0
11	35.04547465924322	37.0	37.0	37.0	33.0	37.0
12	34.957047971423904	37.0	37.0	37.0	33.0	37.0
13	34.90562885956234	37.0	37.0	37.0	33.0	37.0
14	36.361282987150716	40.0	37.0	40.0	27.0	40.0
15	36.528513653217	40.0	37.0	40.0	33.0	40.0
16	36.61330769724983	40.0	37.0	40.0	33.0	40.0
17	36.538804428816064	40.0	37.0	40.0	33.0	40.0
18	36.49146491415685	40.0	37.0	40.0	33.0	40.0
19	36.62006345236608	40.0	37.0	40.0	33.0	40.0
20	36.60242784427999	40.0	37.0	40.0	33.0	40.0
21	36.61883328748839	40.0	37.0	40.0	33.0	40.0
22	36.51671847747697	40.0	37.0	40.0	33.0	40.0
23	36.431789262715995	40.0	37.0	40.0	27.0	40.0
24	36.479165212283405	40.0	37.0	40.0	33.0	40.0
25	35.42736144791769	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.329794753680396
1101	2	0.3280867995425112
1101	3	0.3118005164905284
1101	4	0.4260027314018231
1101	5	0.24466786899711224
1101	6	0.3035888275861538
1101	7	0.31055918571416896
1101	8	0.313886429145235
1101	9	0.4008146817109832
1101	10	0.5167405459154608
1101	11	0.36761296457980563
1101	12	0.4179674440146144
1101	13	0.40903288374348534
1101	14	0.8657413827748783
1101	15	0.6588596328679373
1101	16	0.6499615212761611
1101	17	0.5146303905263565
1101	18	0.6964243440201443
1101	19	0.6187139648575695
1101	20	0.5667294684438886
1101	21	0.6026042310148085
1101	22	0.682504095917885
1101	23	0.7215248002296022
1101	24	0.6716625520984394
1101	25	0.7402572562434031
1102	1	0.3171716575076111
1102	2	0.31776048438981874
1102	3	0.27870677422011525
1102	4	0.3674131591255616
1102	5	0.2484636930251085
1102	6	0.20453719296777706
1102	7	0.22180562361671718
1102	8	0.2898478330295404
1102	9	0.35568653569121267
1102	10	0.5133542937413296
1102	11	0.29148292820086397
1102	12	0.461437292946961
1102	13	0.339946072909342
1102	14	0.7917862796105197
1102	15	0.7628267024604298
1102	16	0.6671495073116276
1102	17	0.6640117551859817
1102	18	0.6854671693715062
1102	19	0.6157829595666371
1102	20	0.5566736597148747
1102	21	0.5812716656137837
1102	22	0.6001569936154851
1102	23	0.6353270710231058
1102	24	0.6587509085813039
1102	25	0.6766495159725281
1103	1	0.3274588801769305
1103	2	0.347470505884214
1103	3	0.3236030245920709
1103	4	0.3852948875364888
1103	5	0.18390533974154977
1103	6	0.20743895754834796
1103	7	0.27000549613815394
1103	8	0.3984911134102447
1103	9	0.3981830176323129
1103	10	0.5205831510104275
1103	11	0.3652294101419642
1103	12	0.3930309482679206
1103	13	0.3470677822491979
1103	14	0.7936905681200912
1103	15	0.7037648054757284
1103	16	0.7177690844006506
1103	17	0.6324805711231676
1103	18	0.7197869469772087
1103	19	0.6828707718383953
1103	20	0.6351613142445842
1103	21	0.6542437378336032
1103	22	0.6848654977092252
1103	23	0.7309923490638823
1103	24	0.7781859790495602
1103	25	0.8433176267763045
1104	1	0.3558545015660286
1104	2	0.3964866753229792
1104	3	0.43209744675807116
1104	4	0.44179456681663964
1104	5	0.4126988612006244
1104	6	0.3605186250313892
1104	7	0.38373460449211905
1104	8	0.5039572000898289
1104	9	0.48853952689954383
1104	10	0.652529090652294
1104	11	0.44788527886661456
1104	12	0.5615001221563887
1104	13	0.4391185838245377
1104	14	0.8122586773532348
1104	15	0.7869492732153489
1104	16	0.6926255174128144
1104	17	0.6919540579086174
1104	18	0.7238790687072125
1104	19	0.6322703472770854
1104	20	0.6837621458733949
1104	21	0.5740274706089252
1104	22	0.7966243096552574
1104	23	0.7411970714954279
1104	24	0.6836016588859266
1104	25	0.8261323415374946
1105	1	0.22522643260929343
1105	2	0.15926343903849016
1105	3	0.17371787274652206
1105	4	0.24826519433008798
1105	5	0.18903472535655652
1105	6	0.1450407585535629
1105	7	0.08715842534780904
1105	8	0.2848825766411025
1105	9	0.23951292380363753
1105	10	0.34687600992614165
1105	11	0.24362651661112977
1105	12	0.3365313903751428
1105	13	0.3335073343358559
1105	14	0.5741815619859949
1105	15	0.5373632369369261
1105	16	0.4377062087757153
1105	17	0.3679354928070353
1105	18	0.4087375074229058
1105	19	0.41749050976521573
1105	20	0.4577815807055998
1105	21	0.40317295746449133
1105	22	0.441186024337604
1105	23	0.509814637472914
1105	24	0.5215277777943754
1105	25	0.4829937306039156
1106	1	-0.08330123455737137
1106	2	-0.1806963692595609
1106	3	-0.2713385299282898
1106	4	-0.17279654045983506
1106	5	-0.49411074894030094
1106	6	-0.48936433351445174
1106	7	-0.6339710250258719
1106	8	-0.43224883655525304
1106	9	-0.0865846741666303
1106	10	-0.5079566465511078
1106	11	-0.5847657373735657
1106	12	-0.9655829305565575
1106	13	-0.3939479326116242
1106	14	-0.571374419227503
1106	15	-0.29598178700183553
1106	16	-0.31801439414777377
1106	17	-0.4254679781636952
1106	18	-0.526121689726267
1106	19	-0.798715846806644
1106	20	-0.36668262985479316
1106	21	-0.11381738909301475
1106	22	0.03899082839706125
1106	23	-0.6450140921599541
1106	24	-0.14116213459202953
1106	25	-0.07462057054368643
1107	1	-0.9840894985389035
1107	2	-1.0261824848854815
1107	3	-0.7601463906369688
1107	4	-0.8408700216130711
1107	5	-0.9480858107245567
1107	6	-0.8778824301692154
1107	7	-0.9589497584695224
1107	8	-0.9003561534931066
1107	9	-0.9041666334810543
1107	10	-0.7846165576187616
1107	11	-0.8825995609068045
1107	12	-0.8497656547735275
1107	13	-0.8059554969754643
1107	14	-1.04558499945486
1107	15	-0.9450276958914046
1107	16	-1.0127144851374297
1107	17	-0.9464794297419701
1107	18	-1.081458114511861
1107	19	-1.1974142959950385
1107	20	-1.1360510521392513
1107	21	-1.0117265774300392
1107	22	-1.0766593633342367
1107	23	-0.7904577974759874
1107	24	-0.7505567153186945
1107	25	-0.6922998980350741
1108	1	-0.06935084387760071
1108	2	-0.18516167545137918
1108	3	-0.15020708817335304
1108	4	-0.14750347752193704
1108	5	-0.4678322764790792
1108	6	-0.08417205278586692
1108	7	-0.057319505432396056
1108	8	0.159276662788308
1108	9	-0.21562063625008676
1108	10	-0.5574370148395573
1108	11	-0.05818835983541959
1108	12	0.10654581744118019
1108	13	0.07299735097642923
1108	14	-0.23315482703004875
1108	15	0.03599659495933594
1108	16	-0.7351856091852227
1108	17	-0.7669445097601084
1108	18	-0.6336917210401296
1108	19	-0.13936162623296866
1108	20	-1.0234519694593942
1108	21	-0.30765425107831845
1108	22	-0.49713738424830467
1108	23	-0.14084142402319344
1108	24	-0.41762458132235025
1108	25	-1.1400627248696296
1109	1	0.13426416640376004
1109	2	0.11104289029161407
1109	3	0.18115383045127942
1109	4	0.2527599301379766
1109	5	0.048931638848436876
1109	6	0.1488424839994522
1109	7	0.10623791757544154
1109	8	0.2726468780997706
1109	9	0.24765328088795258
1109	10	0.2593782114783991
1109	11	0.17926630229772655
1109	12	0.23650927426582768
1109	13	0.18564491989788223
1109	14	0.45135396030283204
1109	15	0.31132235536077246
1109	16	0.3057808586915982
1109	17	0.3091475581310661
1109	18	0.23676837159560193
1109	19	0.23990490101865447
1109	20	0.19351490559397178
1109	21	0.28059704982468503
1109	22	0.3773726126564796
1109	23	0.451541707032618
1109	24	0.4401694385480539
1109	25	0.3855979673703587
1110	1	0.048494091419243546
1110	2	0.016387688933527045
1110	3	0.10479549910385444
1110	4	-0.9267852469436093
1110	5	-0.28019138054887804
1110	6	-0.05319161799896932
1110	7	0.14511958514989942
1110	8	0.15369066079944815
1110	9	0.16639932603767704
1110	10	-0.05656846898941126
1110	11	0.09598989196570074
1110	12	0.06244745512018568
1110	13	0.1835553977875719
1110	14	0.30537010202640147
1110	15	0.15525683835593895
1110	16	0.058466866648785754
1110	17	0.19604152197523916
1110	18	0.2933507914190514
1110	19	0.15256032274710662
1110	20	0.17272680480040492
1110	21	0.2757615218671461
1110	22	-0.20848885262856243
1110	23	0.1946121272630421
1110	24	0.2367422316661063
1110	25	0.3707476970741226
1111	1	-0.019379666916968574
1111	2	-0.135141742188857
1111	3	-0.09599059980628155
1111	4	-0.03051001227164818
1111	5	-0.1334805901861671
1111	6	-0.020869754243939553
1111	7	-0.04220337164828436
1111	8	0.06558614372004001
1111	9	-0.05616162346744602
1111	10	-0.06038597682827174
1111	11	-0.10994494613667172
1111	12	0.02645265502373917
1111	13	-0.1437515384888215
1111	14	-0.28275483289547054
1111	15	-0.1286716132129655
1111	16	-0.14094282418616189
1111	17	0.0010987281593415332
1111	18	-0.06356668678699862
1111	19	-0.09526845526178818
1111	20	-0.10894858874752344
1111	21	-0.09100840359695184
1111	22	-0.06020127791557428
1111	23	0.03307930055166253
1111	24	0.07695818418030598
1111	25	0.015257042847530045
1112	1	-0.12832955373111687
1112	2	-0.34836975478901877
1112	3	-0.14736218476753749
1112	4	-0.14313748110062363
1112	5	-0.05692850672807026
1112	6	-0.15505565452061632
1112	7	-0.0744818807091363
1112	8	-0.042485384947717364
1112	9	-0.10632924632499652
1112	10	0.14220466168366386
1112	11	-0.07605009519465256
1112	12	-0.01789038793897646
1112	13	-0.022629980476580158
1112	14	-0.3229555730950153
1112	15	-0.17875623311211797
1112	16	-0.11640964096880424
1112	17	-0.24416890547781378
1112	18	-0.20288469711146462
1112	19	-0.1731614018069223
1112	20	-0.9766151635879226
1112	21	-1.2425258126590109
1112	22	-1.6699810745403596
1112	23	-0.5630306394418696
1112	24	-0.3369187007533938
1112	25	-1.5165173999067818
1113	1	-0.3403156878346465
1113	2	-0.4151237730812021
1113	3	-0.39987536382662725
1113	4	-0.5962547835321459
1113	5	-0.3492245395084126
1113	6	-0.29693811727290154
1113	7	-0.27243903849388573
1113	8	-0.20995629709123875
1113	9	-0.25047545463044685
1113	10	-0.2115809863503415
1113	11	-0.3276174346097278
1113	12	-0.2746239185179178
1113	13	-0.37468614851105286
1113	14	-0.6889964545950207
1113	15	-0.5908898317877558
1113	16	-0.573859771878837
1113	17	-0.5516440443128943
1113	18	-1.087802452738245
1113	19	-1.126537706504422
1113	20	-1.327887867379033
1113	21	-1.2261354072488402
1113	22	-0.644170371618074
1113	23	-2.1184713894447995
1113	24	-1.1190564087124883
1113	25	-0.6784450060730549
1114	1	-0.3672754288947999
1114	2	-0.44866656840752484
1114	3	-0.3711865700432533
1114	4	-0.6892279801548966
1114	5	-0.7449439293964844
1114	6	-0.4740160793714807
1114	7	-0.4249438332771618
1114	8	-0.2220372545825242
1114	9	-1.1719316033743183
1114	10	-0.2742284825037373
1114	11	-0.4037301475735333
1114	12	-0.4470923375970557
1114	13	-0.44201016380263525
1114	14	-0.7159251642488016
1114	15	-1.3024307566098585
1114	16	-1.1328944365457048
1114	17	-1.6096395878581475
1114	18	-1.7744022294845152
1114	19	-1.7530828334961441
1114	20	-0.9807341800290743
1114	21	-0.758795272865612
1114	22	-0.5770616798073505
1114	23	-0.46907094207536204
1114	24	-0.9845332795399528
1114	25	-0.6628290542062132
1115	1	-0.6993052919445866
1115	2	-0.4704991432669843
1115	3	-0.5244782616736963
1115	4	-0.6450893047859623
1115	5	-0.4097940830033764
1115	6	-0.4242235655298643
1115	7	-0.3721521820136715
1115	8	-0.14793618422079646
1115	9	-0.5780387415270454
1115	10	-0.17788649364501907
1115	11	-0.5291171392154439
1115	12	-0.5079385989794787
1115	13	-0.4790150205849244
1115	14	-0.5163217802230733
1115	15	-1.576145833880716
1115	16	-0.936405936879261
1115	17	-0.730508454736416
1115	18	-2.2212454059815983
1115	19	-1.2144751960679017
1115	20	-0.976014985328618
1115	21	-0.838799267069767
1115	22	-0.7592076722183592
1115	23	-0.4813691816511252
1115	24	-0.48939991986186726
1115	25	-0.6508036102759149
1116	1	-1.487218794419622
1116	2	-0.8483686034448255
1116	3	-1.616531202095267
1116	4	-0.9395867356052605
1116	5	-0.9395840821123329
1116	6	-0.46248096106600656
1116	7	-1.1154602354382845
1116	8	-2.814225641697476
1116	9	-1.318899334402829
1116	10	-0.7412272075236359
1116	11	-0.4555570320434299
1116	12	-0.7311512825334177
1116	13	-1.5935044263921867
1116	14	-1.7108894859382602
1116	15	-1.5415496819636516
1116	16	-1.0569542267727314
1116	17	-1.7497563788529789
1116	18	-2.4232865162352937
1116	19	-1.3180315877490116
1116	20	-0.9754091305443495
1116	21	-0.6804027339646339
1116	22	-0.525555019621244
1116	23	-0.48118071715759925
1116	24	-0.5328547435833997
1116	25	-0.6048784614461269
1201	1	0.29744170642797485
1201	2	0.3154776412277549
1201	3	0.37172271631632015
1201	4	0.39487781651170195
1201	5	0.33031101188225165
1201	6	0.1929860583866585
1201	7	0.1656701318372953
1201	8	0.25380783479430846
1201	9	0.24187813533780655
1201	10	0.42909440191665027
1201	11	0.3522746434766688
1201	12	0.38713280435772646
1201	13	0.4191270519727013
1201	14	0.768693206485132
1201	15	0.6444072559662359
1201	16	0.6002569149419088
1201	17	0.6030692176074908
1201	18	0.532416104983156
1201	19	0.5125847056750814
1201	20	0.5448117939681794
1201	21	0.5176727506147571
1201	22	0.5726267962207316
1201	23	0.6723988499953393
1201	24	0.6085198928232032
1201	25	0.7245368195627861
1202	1	0.3858273510700698
1202	2	0.4218100835214891
1202	3	0.44787869957498216
1202	4	0.4876561173154812
1202	5	0.3028916549614209
1202	6	0.07324413099332361
1202	7	0.20912395991122423
1202	8	0.26796173598941664
1202	9	0.2579563501112361
1202	10	0.33544587600472653
1202	11	0.24600154644863892
1202	12	0.31265490161720066
1202	13	0.3228641624041373
1202	14	0.5686398732009792
1202	15	0.46303307558282114
1202	16	0.4201057855284702
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2210	20	0.4832853351003621
2210	21	0.5558041724512464
2210	22	0.5305312035906198
2210	23	0.6575116545242423
2210	24	0.616654719870084
2210	25	0.6782896242713292
2211	1	0.0032365182952851512
2211	2	-0.0464720074075089
2211	3	0.04515945663090548
2211	4	0.06665830062016909
2211	5	0.05259538250623308
2211	6	-0.10048298246552179
2211	7	-0.02785554755116948
2211	8	0.12472132029882488
2211	9	-0.011521747161957308
2211	10	0.1695371500857874
2211	11	-0.04018443925701831
2211	12	0.032166047088999505
2211	13	-0.023428201338411725
2211	14	-0.07613721736303347
2211	15	-0.09701463858344539
2211	16	-0.04856565034275917
2211	17	0.008407018765332452
2211	18	0.03494709780938621
2211	19	-0.01840675069974651
2211	20	0.023445814938071408
2211	21	0.058147820704611775
2211	22	0.020253991699057394
2211	23	0.12009313500932706
2211	24	0.14958854493028184
2211	25	0.15671517936529966
2212	1	0.1679410934236536
2212	2	0.1620263030115261
2212	3	0.21762977461586175
2212	4	0.1956532101626749
2212	5	0.2696574474633877
2212	6	0.2095859637109072
2212	7	0.20420784338284648
2212	8	0.27838690708747293
2212	9	0.18099311348110803
2212	10	0.3240970782335495
2212	11	0.2611932368368599
2212	12	0.2820970916723198
2212	13	0.13902914332594207
2212	14	0.201880742924196
2212	15	0.26201738907079886
2212	16	0.19597520591487694
2212	17	0.2034960994614181
2212	18	0.17104302923189607
2212	19	0.16634421513810338
2212	20	0.1387883604994684
2212	21	0.2403697282447581
2212	22	-0.3377456682594939
2212	23	0.21886154737611463
2212	24	0.20532681373119743
2212	25	0.3670710124160408
2213	1	-0.04527733893844754
2213	2	-0.8755479195120834
2213	3	-0.22064466497018742
2213	4	-0.1496775664517287
2213	5	0.0019571364860624385
2213	6	-0.10329534506581695
2213	7	-0.08464448140746583
2213	8	0.06194990567963288
2213	9	-0.24150108958933458
2213	10	-1.4159083714754743
2213	11	-0.49332878708629835
2213	12	-0.19761938677509505
2213	13	-0.18413617641898128
2213	14	-0.17043954123668925
2213	15	-0.15579165153791763
2213	16	-0.1376036435704151
2213	17	-0.22130188432260667
2213	18	-0.07655365350024113
2213	19	-0.10220496738349993
2213	20	-0.1254912219187858
2213	21	-0.08506420924496183
2213	22	-0.04847271058864777
2213	23	-0.956659316499767
2213	24	-1.499240866937015
2213	25	-1.4597573186634847
2214	1	-0.04119450332020591
2214	2	-0.3010038308713696
2214	3	-0.06712453332298907
2214	4	-0.0689213213672204
2214	5	-0.1514606878119764
2214	6	-0.1860877367595961
2214	7	-0.12100578706063203
2214	8	-0.11861009900346886
2214	9	-0.4930570556390137
2214	10	-0.1369589690316957
2214	11	-0.25603216190086187
2214	12	-0.22556484923412512
2214	13	-0.1543546832357663
2214	14	-0.15444474251521711
2214	15	-0.21151668949326563
2214	16	-0.33035242387710895
2214	17	-0.2530943199204714
2214	18	-0.09790008867857836
2214	19	-0.8486608510994742
2214	20	-0.4432013422430714
2214	21	-0.2703108390647273
2214	22	-0.6579424685362412
2214	23	-1.8419601488886173
2214	24	-1.3696531750395948
2214	25	-1.6630247850348567
2215	1	-0.3538238456438698
2215	2	-0.17727359011056265
2215	3	-1.08628255597446
2215	4	-0.7734600005783889
2215	5	-0.21359316566498165
2215	6	-0.24663180603729273
2215	7	-0.13241433733381314
2215	8	-0.13473777888660976
2215	9	-0.36984118395152876
2215	10	-1.2789117197180673
2215	11	-0.5286590563618176
2215	12	-0.5012716000500461
2215	13	-0.4607395913673997
2215	14	-0.7220654795682648
2215	15	-1.004154265536954
2215	16	-0.6720215244981702
2215	17	-0.5092434424451397
2215	18	-0.4795186266289946
2215	19	-0.9939619282782814
2215	20	-1.186796863131164
2215	21	-0.7070270312394342
2215	22	-0.3755328656026151
2215	23	-0.7419374584729113
2215	24	-1.7879768579920992
2215	25	-0.7556393527585925
2216	1	-0.5462893423493647
2216	2	-0.526948700988811
2216	3	-1.386111324363867
2216	4	-0.7678258484430316
2216	5	-0.5854133081136013
2216	6	-0.506750816276508
2216	7	-0.42603715748157356
2216	8	-0.39630002624265614
2216	9	-0.2994134881705932
2216	10	-0.42623828522665974
2216	11	-0.48048766874529036
2216	12	-0.36666550617509586
2216	13	-1.0734142245425673
2216	14	-0.7601894030570406
2216	15	-1.2216974814190635
2216	16	-0.9040864400451198
2216	17	-1.9173491794747548
2216	18	-0.9564628661724583
2216	19	-0.6105679786215461
2216	20	-0.613914495248558
2216	21	-0.48142649432075046
2216	22	-0.511747288986264
2216	23	-0.3353577260595344
2216	24	-0.546269567818257
2216	25	-0.47446508736376103
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4804.0
3	453.0
4	595.0
5	551.0
6	370.0
7	256.0
8	351.0
9	727.0
10	1366.0
11	2461.0
12	4423.0
13	7016.0
14	9557.0
15	12161.0
16	13734.0
17	14646.0
18	15345.0
19	16025.0
20	16862.0
21	17775.0
22	19792.0
23	20896.0
24	22454.0
25	24104.0
26	25583.0
27	29054.0
28	32823.0
29	39258.0
30	47747.0
31	61545.0
32	82838.0
33	118837.0
34	175102.0
35	282847.0
36	522025.0
37	1951070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46053312918216	21.776639719307017	13.087343817656697	22.675483333854128
2	14.818186327041143	21.535297503910673	41.43181715592603	22.21469901312215
3	17.74041800388597	27.002066721437384	30.620612815233546	24.6369024594431
4	11.420498288808153	17.235428667634086	40.06519197738406	31.278881066173703
5	12.337768250993348	38.868592736378304	35.669669406763425	13.123969605864922
6	30.12198279237509	39.59152722478348	17.781546824632212	12.504943158209208
7	24.89297583742471	33.860293687343976	24.326323234428756	16.920407240802554
8	22.9458000902556	38.37262295095676	21.839932054647285	16.841644904140356
9	23.318072285798305	16.97112108073303	23.162423537970742	36.54838309549792
10	13.806285323373515	30.76560249820613	34.87595108627475	20.552161092145603
11	31.686359232987645	26.575293827804586	23.41985688470624	18.31849005450153
12	19.202540063356654	28.57685547871175	33.192231089577255	19.028373368354337
13	25.59282263014502	24.9520139105304	27.76285868312679	21.692304776197783
14	19.049743891895567	23.880596598937885	29.468290700675432	27.601368808491117
15	21.992935225633843	31.726800855576226	24.6934069073149	21.586857011475033
16	20.7832394767367	32.703984116375864	26.39579520960687	20.116981197280566
17	18.48100362698315	31.549653215373237	29.88724413381322	20.0820990238304
18	18.72010314493385	31.349142312304956	30.608582423276058	19.322172119485135
19	19.563925042509673	29.6348641460287	30.804204116658635	19.997006694802995
20	21.22318709552636	29.469857873874318	29.62790584900134	19.67904918159799
21	21.561244944943745	30.146431160243225	28.663252364181986	19.62907153063104
22	19.735464009582802	29.75276010048789	30.7248082129398	19.786967676989512
23	19.794762397019568	30.280977463663678	30.002321667118643	19.921938472198107
24	19.86098013575845	28.99084880669878	30.366106270249688	20.782064787293084
25	20.707037624960105	29.35463235632288	29.07818359954789	20.86014641916912
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6910.0
1	6910.0
2	5497.5
3	4085.0
4	4085.0
5	4085.0
6	6250.5
7	8416.0
8	8416.0
9	8416.0
10	13749.5
11	19083.0
12	19083.0
13	19083.0
14	31526.5
15	43970.0
16	43970.0
17	43970.0
18	67677.0
19	91384.0
20	91384.0
21	91384.0
22	126376.0
23	161368.0
24	161368.0
25	161368.0
26	204777.0
27	248186.0
28	248186.0
29	248186.0
30	292470.5
31	336755.0
32	336755.0
33	336755.0
34	373986.0
35	411217.0
36	411217.0
37	411217.0
38	433753.0
39	456289.0
40	456289.0
41	456289.0
42	443020.5
43	429752.0
44	429752.0
45	429752.0
46	416269.5
47	402787.0
48	402787.0
49	402787.0
50	371145.5
51	339504.0
52	339504.0
53	339504.0
54	300434.5
55	261365.0
56	261365.0
57	261365.0
58	222146.0
59	182927.0
60	182927.0
61	182927.0
62	146009.0
63	109091.0
64	109091.0
65	109091.0
66	80687.0
67	52283.0
68	52283.0
69	52283.0
70	36396.5
71	20510.0
72	20510.0
73	20510.0
74	13676.0
75	6842.0
76	6842.0
77	6842.0
78	4420.0
79	1998.0
80	1998.0
81	1998.0
82	1275.5
83	553.0
84	553.0
85	553.0
86	339.5
87	126.0
88	126.0
89	126.0
90	77.0
91	28.0
92	28.0
93	28.0
94	19.5
95	11.0
96	11.0
97	11.0
98	12.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31097611344106013
2	0.1824248571737692
3	0.19916822720252497
4	0.21958290095851624
5	0.2458660980966793
6	0.19914041429550047
7	0.19499629114884828
8	0.21872070084075637
9	0.21938821060934463
10	0.2291505409749481
11	0.2029507825578585
12	0.20890274466110392
13	0.22125167537998688
14	0.20901399628920195
15	0.20103169197316723
16	0.20456393116528013
17	0.20434142790908405
18	0.20837429942763816
19	0.20709490570451067
20	0.20308984709298106
21	0.20064231127482407
22	0.20467518279337818
23	0.20898618338217745
24	0.2239495273613645
25	0.21716317804738372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3595453.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.70945836007968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.13076811885827	75.37376471048786
2	7.1210954770342365	11.7796389967183
3	0.9714416663121392	2.4104224214727092
4	0.25333708577144376	0.8381349258670866
5	0.11870222160809742	0.4908898227671941
6	0.08214382376330155	0.4076442702653108
7	0.05398122559725347	0.31253305515334723
8	0.039223248722005166	0.25953069255357875
9	0.030949254141402628	0.23038164420154778
>10	0.16875599519873388	2.689021984968689
>50	0.01595251892725269	0.9139659615484667
>100	0.011958707502280733	1.940285756681887
>500	8.791414251798848E-4	0.48871734779383813
>1k	6.762626153553113E-4	1.205167163572056
>5k	1.3525252307106152E-4	0.6599012459482086
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6280	0.17466505611393054	No Hit
ATTCCATTCCATTCCATTCCATTCC	6216	0.17288503006436184	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5621	0.15633635038477767	No Hit
CCCATGTACTCTGCGTTGATACCAC	5479	0.15238691758729705	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4634	0.12888501115158507	No Hit
GAATGGAATGGAATGGAATGGAATG	4494	0.12499120416815349	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4307	0.1197901905545699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.5625814049022476E-5	0.0
5	0.0	0.0	0.0	5.5625814049022476E-5	0.0
6	0.0	0.0	0.0	5.5625814049022476E-5	0.0
7	0.0	0.0	0.0	5.5625814049022476E-5	0.0
8	0.0	0.0	0.0	5.5625814049022476E-5	0.0
9	0.0	0.0	0.0	5.5625814049022476E-5	0.0
10	0.0	0.0	0.0	5.5625814049022476E-5	0.0
11	0.0	0.0	0.0	5.5625814049022476E-5	0.0
12	0.0	0.0	0.0	5.5625814049022476E-5	0.0
13	0.0	0.0	0.0	5.5625814049022476E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCGC	95	1.3632645E-5	10.998685	10
TCGGAAC	90	9.547148E-5	10.552823	13
ACGATTC	110	6.0689035E-6	10.361097	18
CAGGACG	75	0.0026369642	10.138905	4
CGAATTG	95	1.6492569E-4	9.99755	19
ACGAATT	105	4.119354E-5	9.949944	18
CTTACAC	540	0.0	9.856306	3
GTCTTAG	450	0.0	9.721195	1
ACTCGGA	160	6.668779E-8	9.497673	11
GAACGAT	120	1.7070322E-5	9.4975395	16
GTGTTAG	565	0.0	9.425708	1
TTAGACA	510	0.0	9.318847	4
TATACTG	1020	0.0	9.318586	5
GGAACGA	245	1.8189894E-12	9.303453	15
CTCGGAA	155	4.280082E-7	9.191297	12
GGCGCCC	105	4.5004708E-4	9.046537	8
GTCTAAG	305	0.0	9.042167	1
CTAGGAC	400	0.0	9.02908	3
TCTGGAC	380	0.0	9.004069	3
GTATTAG	615	0.0	8.968655	1
>>END_MODULE