FastQCFastQC Report
Wed 25 May 2016
SRR1295067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595453
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT150930.4197802057209481No Hit
GGTATCAACGCAGAGTACTTTTTTT134760.37480673506231343No Hit
TATCAACGCAGAGTACTTTTTTTTT98220.2731783727947494No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64060.17816948239901897No Hit
GTACATGGGAAGCAGTGGTATCAAC62760.17455380448583252No Hit
CCCATGTACTCTGCGTTGATACCAC59700.16604305493633206No Hit
ATTCCATTCCATTCCATTCCATTCC58590.16295582225661134No Hit
GAATGGAATGGAATGGAATGGAATG49980.13900890930850718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG400.005282427614.2493735
GCACCGT951.3623347E-510.9993646
AGCGTCA700.001492775210.8566663
GCGTCAG700.001492775210.8566664
AGCCGTG803.775289E-410.6868827
GTCCTAC2150.010.6174751
TATTCCG1856.002665E-1110.2698195
TAGGACC2151.8189894E-1210.1623454
TGGACCG856.600095E-410.0583825
TCGGAAC951.6466095E-49.99914313
TTATACT6550.09.5720994
TATACTG10050.09.2632755
GTCTAGG4100.09.0475131
GTCTTAG4100.09.0475131
GACTGTC2950.09.0164287
CTTACAC5300.08.961873
ACCGTTC850.00744224958.94065958
CCGTTCA850.00744394778.9404129
GGTATCA54650.08.9110371
GTAGGAC2151.0440999E-98.8368223
Produced by FastQC (version 0.11.5)