FastQCFastQC Report
Wed 25 May 2016
SRR1295066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4301226
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC286390.6658334158679409No Hit
CCCATGTACTCTGCGTTGATACCAC256290.5958533683187073No Hit
GAGTACATGGGAAGCAGTGGTATCA144470.33588097905108916No Hit
CATGTACTCTGCGTTGATACCACTG109270.2540438470333807No Hit
GCGTTGATACCACTGCTTCCCATGT87650.20377910856114048No Hit
ACGCAGAGTACATGGGAAGCAGTGG76070.176856552062133No Hit
GTATCAACGCAGAGTACATGGGAAG74720.173717912055772No Hit
GCTTCCCATGTACTCTGCGTTGATA70290.16341852299786153No Hit
TATCAACGCAGAGTACATGGGAAGC62670.14570264385084625No Hit
ACTCTGCGTTGATACCACTGCTTCC62450.14519116177573557No Hit
GGTATCAACGCAGAGTACATGGGAA61720.1434939712537774No Hit
GTATCAACGCAGAGTACTTTTTTTT55880.12991644707811215No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN50560.11754788053452665No Hit
GGTATCAACGCAGAGTACTTTTTTT46590.10831795399730217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC453.5435238E-516.8876158
TCGGACG550.003071694612.0899969
TCGTCTA1501.7826096E-1011.39608614
GTCCTAG4350.010.2758061
TAGACTG5500.010.0239675
TTAGACT3550.09.907824
CGCAGGA1106.801757E-59.5051552
ACCGGAC800.00449801759.5051543
CGCAAAA1701.6529157E-89.5051542
GTCTAAT4650.09.20379451
CTAGAAC4650.09.1985363
TACACCG1151.1045345E-49.0922045
CTTAGAC3050.09.0376873
CGGACTG950.00181525979.0051985
CCGTCCT950.00182607368.9993219
CGTCCGC950.00182607368.99932110
GTGTAAG7200.08.9822221
ATTAGAC3000.08.8714773
TAGACAG5900.08.8610475
GTGCTAG4550.08.7790041
Produced by FastQC (version 0.11.5)