FastQCFastQC Report
Wed 25 May 2016
SRR1295066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4301226
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC285860.6646012090506288No Hit
GTACATGGGAAGCAGTGGTATCAAC281670.6548598004382937No Hit
GTATCAACGCAGAGTACTTTTTTTT159590.3710337471223321No Hit
GAGTACATGGGAAGCAGTGGTATCA154920.36017637761884636No Hit
GGTATCAACGCAGAGTACTTTTTTT143880.33450927712238326No Hit
CATGTACTCTGCGTTGATACCACTG123980.2882433985100992No Hit
TATCAACGCAGAGTACTTTTTTTTT107200.24923126569029388No Hit
GCGTTGATACCACTGCTTCCCATGT93420.21719388844017962No Hit
ACGCAGAGTACATGGGAAGCAGTGG93070.216380166957049No Hit
GCTTCCCATGTACTCTGCGTTGATA79390.18457528155925776No Hit
GTATCAACGCAGAGTACATGGGAAG78360.1821806154803305No Hit
GGTATCAACGCAGAGTACATGGGAA69880.16246530640333712No Hit
TATCAACGCAGAGTACATGGGAAGC68760.1598613976573191No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67900.15786196772734098No Hit
ACTCTGCGTTGATACCACTGCTTCC67050.1558857869825952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG851.022272E-915.6457959
TCGTCCG759.692976E-713.9322089
CGGACGT1051.375156E-913.57033310
CGTCCGC853.95146E-612.29312410
GTCCGCA951.0421973E-611.99903211
GACGTTC1157.093331E-811.56428312
GCGCGTC855.332088E-511.1755689
TGCGTGA1303.275636E-810.96065417
TCGTCTA1053.4736186E-610.85626614
GGACGTT1303.8509825E-710.22994411
ACCGTTC750.002650974610.1325158
GGACCGC750.002650974610.1325156
TGAACCG1252.5752197E-69.8793175
ATCGTCC1356.458322E-79.8510558
CTCGGAC1356.458322E-79.8510558
TGACTCG1451.6210834E-79.8269075
TTGCGTG1451.6213744E-79.82679316
GTCCCGC1502.5994814E-79.5130571
GCTCGTT1901.0550139E-99.49923311
CTCGTTG1002.7596197E-49.49923212
Produced by FastQC (version 0.11.5)