FastQCFastQC Report
Wed 25 May 2016
SRR1295065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673530
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC344820.9386611787572172No Hit
CCCATGTACTCTGCGTTGATACCAC317030.8630118714152328No Hit
GAGTACATGGGAAGCAGTGGTATCA174070.4738494037070611No Hit
CATGTACTCTGCGTTGATACCACTG127000.3457165178996769No Hit
GCGTTGATACCACTGCTTCCCATGT106030.28863245978663576No Hit
GTATCAACGCAGAGTACATGGGAAG90710.24692870345417078No Hit
ACGCAGAGTACATGGGAAGCAGTGG85090.2316300669927835No Hit
GCTTCCCATGTACTCTGCGTTGATA79810.21725697081553708No Hit
TATCAACGCAGAGTACATGGGAAGC77220.2102065315922287No Hit
GGTATCAACGCAGAGTACATGGGAA73590.20032502797037183No Hit
ACTCTGCGTTGATACCACTGCTTCC71700.19518011286147113No Hit
GTATCAACGCAGAGTACTTTTTTTT59900.16305842064717044No Hit
ATTCCATTCCATTCCATTCCATTCC55720.15167971950685036No Hit
GTGGTATCAACGCAGAGTACATGGG47450.12916731318377692No Hit
GGTATCAACGCAGAGTACTTTTTTT46870.12758845034612484No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN42830.1165908540286863No Hit
GAATGGAATGGAATGGAATGGAATG40370.1098942978551965No Hit
CAGTGGTATCAACGCAGAGTACATG38930.1059743625341293No Hit
TATCAACGCAGAGTACTTTTTTTTT38740.10545714884593292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG701.0855628E-412.2216425
CAGAACG856.5610826E-410.0648814
GTGCGTT856.6189567E-410.05527111
GTTCTAG3450.09.924841
CTAGGAC3400.09.7839653
CCGGGAC800.0045006069.5044233
GACTCCG1201.7029106E-59.4994957
GCTACAC3050.09.3486133
TATTCCG2451.8189894E-129.3117265
TAGGACT4200.09.2793944
GTCTTAG3500.09.23955251
TATACTG7850.09.2029915
GTCCTAA2750.08.9925071
TCTAGAC2553.6379788E-128.9453393
GTACCGT850.0074428548.9405796
GGGTCGG850.00745952878.93814117
TCAGGAC3950.08.9028783
CTAGACT3300.08.6415644
TAGGGTG3000.08.5551495
ACGCAAA1803.9655606E-78.45449351
Produced by FastQC (version 0.11.5)