##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295065_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3673530
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0396395292811	33.0	33.0	33.0	27.0	33.0
2	30.95880039090466	33.0	33.0	33.0	27.0	33.0
3	30.98007910647252	33.0	33.0	33.0	27.0	33.0
4	34.52677152493651	37.0	37.0	37.0	27.0	37.0
5	34.7402234363133	37.0	37.0	37.0	27.0	37.0
6	34.842879464711054	37.0	37.0	37.0	33.0	37.0
7	34.686379041412486	37.0	37.0	37.0	27.0	37.0
8	34.58719651125757	37.0	37.0	37.0	27.0	37.0
9	34.57278938786399	37.0	37.0	37.0	27.0	37.0
10	34.47055665803737	37.0	37.0	37.0	27.0	37.0
11	34.66196492202323	37.0	37.0	37.0	27.0	37.0
12	34.56848671441366	37.0	37.0	37.0	27.0	37.0
13	34.48155234883069	37.0	37.0	37.0	27.0	37.0
14	35.82612609669718	40.0	37.0	40.0	27.0	40.0
15	35.98646424556217	40.0	37.0	40.0	27.0	40.0
16	36.06600681088762	40.0	37.0	40.0	27.0	40.0
17	35.98835343661274	40.0	37.0	40.0	27.0	40.0
18	35.92311237420138	37.0	37.0	40.0	27.0	40.0
19	36.01139966190558	40.0	37.0	40.0	27.0	40.0
20	36.05116522799596	40.0	37.0	40.0	27.0	40.0
21	36.03962945722507	40.0	37.0	40.0	27.0	40.0
22	35.9506776860404	40.0	37.0	40.0	27.0	40.0
23	35.853195972266455	37.0	37.0	40.0	27.0	40.0
24	35.92402865908268	37.0	37.0	40.0	27.0	40.0
25	34.78405947412979	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3270158408154167
1101	2	0.31402541258417216
1101	3	0.29515590010115744
1101	4	0.3445643325523733
1101	5	0.11741202172948562
1101	6	0.2335009842624416
1101	7	0.19209129086598864
1101	8	0.40871094362475446
1101	9	0.32355544071081965
1101	10	0.537173580370812
1101	11	0.4493501828844302
1101	12	0.3669386134800732
1101	13	0.3604416729394231
1101	14	0.8207013983410576
1101	15	0.6776353608793997
1101	16	0.5603501429422053
1101	17	0.5596611695994298
1101	18	0.5901581900137529
1101	19	0.5164547183261945
1101	20	0.5186864507343714
1101	21	0.4606948111742284
1101	22	0.5526843489029005
1101	23	0.7108553279396546
1101	24	0.6734438395333342
1101	25	0.6988405292757491
1102	1	0.3898914198635026
1102	2	0.3701679572711889
1102	3	0.4293060915289786
1102	4	0.5831434882474298
1102	5	0.3054238950513337
1102	6	0.3456356799550875
1102	7	0.3651107940741767
1102	8	0.42044586438932896
1102	9	0.5226857503532543
1102	10	0.7312196760341578
1102	11	0.5199418653359515
1102	12	0.5353762953948475
1102	13	0.4905043326516818
1102	14	0.8639622704428973
1102	15	0.7552858226268953
1102	16	0.7939027787859487
1102	17	0.7140898745672146
1102	18	0.8108110023107713
1102	19	0.7520217867773553
1102	20	0.7406843789317605
1102	21	0.8176136984165652
1102	22	0.7968475182682297
1102	23	0.8742431530525465
1102	24	0.7310160965665062
1102	25	0.8549339367168685
1103	1	0.32720480529009777
1103	2	0.36539729467033766
1103	3	0.326021357395188
1103	4	0.3351236673974185
1103	5	0.14559796277256964
1103	6	0.08211398501829592
1103	7	0.13301721679192013
1103	8	0.2206043676610392
1103	9	0.31736874380983693
1103	10	0.36121364083944485
1103	11	0.2670637127408142
1103	12	0.2937715688844591
1103	13	0.3155059208608151
1103	14	0.6157429705254884
1103	15	0.535628558158308
1103	16	0.42811280356200854
1103	17	0.43697787405898936
1103	18	0.49259583173711263
1103	19	0.45229749988325807
1103	20	0.525024319215099
1103	21	0.47135996612509956
1103	22	0.496962504609634
1103	23	0.5550201049615779
1103	24	0.586259410206047
1103	25	0.48186774016010503
1104	1	0.30153924701584245
1104	2	0.2627667448658748
1104	3	0.244623214475304
1104	4	0.30860506522664366
1104	5	0.11431912888503604
1104	6	0.14898382210552796
1104	7	0.21560803177403898
1104	8	0.3485313601532454
1104	9	0.4286704775028767
1104	10	0.47901285450821973
1104	11	0.331717183263855
1104	12	0.4756462159764965
1104	13	0.4695316857561451
1104	14	0.6533894142588821
1104	15	0.6753513554898021
1104	16	0.4054074895523456
1104	17	0.5469124161674443
1104	18	0.6714637029962844
1104	19	0.5883269454779239
1104	20	0.6003758678275162
1104	21	0.5428420749985108
1104	22	0.6313107489578726
1104	23	0.5876221084331448
1104	24	0.5530652533162339
1104	25	0.5640371568947415
1105	1	0.16681366648774443
1105	2	0.06271643624181777
1105	3	0.03853952977317121
1105	4	0.15753280550749338
1105	5	0.012382317220470895
1105	6	0.05132651530559684
1105	7	0.08244089280437805
1105	8	0.16795812328582116
1105	9	0.024878262674491225
1105	10	0.34001772529773433
1105	11	0.04051992596338749
1105	12	0.2031173632769807
1105	13	0.09411789732325815
1105	14	0.3746768576836743
1105	15	0.25505569557610386
1105	16	0.19525714416429452
1105	17	0.17364800213098874
1105	18	0.215539756970756
1105	19	0.22270940711759835
1105	20	0.2402077736976196
1105	21	0.2696846459451052
1105	22	0.32911549872524404
1105	23	0.43885880347317396
1105	24	0.47840028940299817
1105	25	0.3691280378409658
1106	1	-0.05933637867954289
1106	2	-0.24690634677378753
1106	3	-0.26077149609468364
1106	4	-0.08296629769274233
1106	5	-0.4915667063993041
1106	6	-0.4311672800370303
1106	7	-0.7145156362532461
1106	8	-0.17717804252581715
1106	9	0.08286363333614588
1106	10	-0.2964947990393014
1106	11	-0.4706178221060142
1106	12	-0.7337049170070742
1106	13	-0.21359413176335096
1106	14	-0.4917438919623791
1106	15	-0.36306714074251545
1106	16	-0.2484108884309677
1106	17	-0.356911941311715
1106	18	-0.5701568198538425
1106	19	-0.6787467563000789
1106	20	-0.2490950042308242
1106	21	-0.010333346158567736
1106	22	0.11425072939635328
1106	23	-0.7006035655370155
1106	24	-0.020643361086357004
1106	25	0.07556221610493452
1107	1	-0.9955223467992909
1107	2	-1.050810688662331
1107	3	-0.8640142730041127
1107	4	-0.8788490225982031
1107	5	-0.9487908796707387
1107	6	-0.8322759853892308
1107	7	-1.0560542032486921
1107	8	-1.0587089675301158
1107	9	-0.9685646797606324
1107	10	-0.8436790035068
1107	11	-0.9028908369360522
1107	12	-0.8719038162821491
1107	13	-0.7816509457872769
1107	14	-1.1513796590908711
1107	15	-0.9540389577990638
1107	16	-0.9348554674678908
1107	17	-1.1250131818482245
1107	18	-1.1293049206813777
1107	19	-1.2296375559177974
1107	20	-1.297921326179491
1107	21	-1.199914633447598
1107	22	-1.1776034945676201
1107	23	-0.9173974541238437
1107	24	-0.8436494900951885
1107	25	-0.8188608583775192
1108	1	-0.0033079856534143914
1108	2	-0.06645695968780885
1108	3	-0.016536735207015596
1108	4	-0.07121664281419271
1108	5	-0.4043341904496742
1108	6	-0.11375649106234675
1108	7	0.011028806482279663
1108	8	0.1376341971791888
1108	9	-0.16868220854365745
1108	10	-0.5077097480398365
1108	11	-0.10686630705583866
1108	12	0.13464684997769893
1108	13	0.20293120291505318
1108	14	-0.22005667966959663
1108	15	0.046701709669967784
1108	16	-0.6907997259888177
1108	17	-0.6881373501428953
1108	18	-0.6194911774308522
1108	19	-0.13200663819358027
1108	20	-0.9271437985653677
1108	21	-0.28467570628836114
1108	22	-0.2439164282931756
1108	23	-0.005044282207116169
1108	24	-0.32811651475887516
1108	25	-1.118845035407368
1109	1	0.2312980364522943
1109	2	0.13931811203943312
1109	3	0.17485277501970842
1109	4	0.1947596827230882
1109	5	0.12880996788112498
1109	6	0.12120083048445451
1109	7	0.23020112483789745
1109	8	0.334097343395392
1109	9	0.2838502252913173
1109	10	0.30452143138861487
1109	11	0.22224889792600067
1109	12	0.2334267412982527
1109	13	0.2636796117931226
1109	14	0.6182653204616315
1109	15	0.4688214061787406
1109	16	0.39797870394514945
1109	17	0.4253431358725237
1109	18	0.40138893518538765
1109	19	0.4818441154643551
1109	20	0.45625675855098535
1109	21	0.5114812943498777
1109	22	0.5300404101013285
1109	23	0.537382812496702
1109	24	0.6204611982009354
1109	25	0.45070554348066594
1110	1	-0.021810394881171646
1110	2	0.0267000135742137
1110	3	0.0890930639198011
1110	4	-0.9592879084082426
1110	5	-0.34227287984835897
1110	6	0.018216154852190414
1110	7	0.07863152031679732
1110	8	0.17008029547596948
1110	9	0.131014455145106
1110	10	-0.13901108745906754
1110	11	0.02095172009533286
1110	12	0.05902148387936279
1110	13	0.04505810139905719
1110	14	0.26248620474916606
1110	15	0.07372186227507171
1110	16	0.08938070185812563
1110	17	0.15014598266402146
1110	18	0.24132925574254926
1110	19	0.20462941016824487
1110	20	0.09573641008869771
1110	21	0.23006839233437404
1110	22	-0.4245843363489712
1110	23	0.03525522277234927
1110	24	0.1589432416063019
1110	25	0.386369169505123
1111	1	0.04468325125859707
1111	2	-0.24161924320744887
1111	3	-0.15132480444425767
1111	4	-0.11766176214026558
1111	5	-0.04956448755397247
1111	6	-0.008670115695238678
1111	7	0.03217580660090391
1111	8	0.18789976967757838
1111	9	0.09781213653090504
1111	10	0.17154354819961526
1111	11	0.02651432897513928
1111	12	0.10912610042671389
1111	13	-0.023542300970945007
1111	14	-0.09865090302839263
1111	15	0.029871632213492205
1111	16	-0.08232381908342035
1111	17	0.09304631794923068
1111	18	-0.11928565100392063
1111	19	-0.10156572569566436
1111	20	0.009219719342311805
1111	21	-0.12525902661359822
1111	22	0.028494871868915084
1111	23	0.052247396868345675
1111	24	0.04997057673730865
1111	25	0.027794327349234038
1112	1	-0.1794600571197691
1112	2	-0.513872136864336
1112	3	-0.12093190613207483
1112	4	-0.3172316343178707
1112	5	-0.17087726602586173
1112	6	-0.22781288036547664
1112	7	-0.23740581921234138
1112	8	-0.07274504844413343
1112	9	-0.15259694420305436
1112	10	0.12112688097357704
1112	11	-0.07272758451982497
1112	12	-0.16712209817416834
1112	13	-0.1737951473836432
1112	14	-0.3220660395596866
1112	15	-0.28077042523630524
1112	16	-0.22921590091939237
1112	17	-0.3339759556316082
1112	18	-0.16231345769678995
1112	19	-0.05875288865411221
1112	20	-0.9438855052742667
1112	21	-1.3178370151666243
1112	22	-1.8024906811479369
1112	23	-0.6657525524598285
1112	24	-0.46869909188751535
1112	25	-1.4867563273813147
1113	1	-0.14751477142948133
1113	2	-0.11999575143699204
1113	3	-0.1474639033757903
1113	4	-0.4646949267068905
1113	5	-0.25926673866356253
1113	6	-0.2966063475591767
1113	7	-0.1546630553169237
1113	8	0.004446393360204581
1113	9	-0.10704773389235811
1113	10	-0.03343564109555075
1113	11	-0.18009804084950787
1113	12	-0.17684067601349795
1113	13	-0.17899596878392998
1113	14	-0.49965234315554596
1113	15	-0.34609555370865763
1113	16	-0.47698470210692534
1113	17	-0.45505236025695694
1113	18	-0.7717919233649297
1113	19	-0.9825929007214285
1113	20	-1.1345440859247375
1113	21	-0.9863603664523737
1113	22	-0.4222650086178845
1113	23	-1.9437206357791652
1113	24	-1.0784473169292497
1113	25	-0.4619228872019505
1114	1	-0.2830645549792621
1114	2	-0.37411448984144613
1114	3	-0.322766521392694
1114	4	-0.49368315078439906
1114	5	-0.674153720729116
1114	6	-0.46731993754062273
1114	7	-0.37488051048080706
1114	8	-0.22741541348374028
1114	9	-1.1314022999612092
1114	10	-0.35567819417739344
1114	11	-0.3572081727810712
1114	12	-0.37153777454988557
1114	13	-0.3281599040550134
1114	14	-0.7692864907539771
1114	15	-1.3197481253433736
1114	16	-1.30556775199355
1114	17	-1.4199897185336
1114	18	-1.7944306834144044
1114	19	-1.554516473180712
1114	20	-0.8694916740508987
1114	21	-0.6297848150196828
1114	22	-0.47614776475063536
1114	23	-0.3128607872943121
1114	24	-0.877170724474091
1114	25	-0.3793338591664934
1115	1	-0.7749729862114556
1115	2	-0.47841010930612526
1115	3	-0.4917309456257577
1115	4	-0.5433550521743982
1115	5	-0.35628288055338686
1115	6	-0.37883441358874137
1115	7	-0.27421684218600717
1115	8	-0.052370689378399504
1115	9	-0.406520316626235
1115	10	-0.16513866561244583
1115	11	-0.3967807454273995
1115	12	-0.4567765243685855
1115	13	-0.4129957860336688
1115	14	-0.40307472628202135
1115	15	-1.496217032221324
1115	16	-0.8810375232353778
1115	17	-0.5755200244880072
1115	18	-2.2027222167421243
1115	19	-1.1307900063353244
1115	20	-0.9209732276601699
1115	21	-0.8244433035642302
1115	22	-0.577355681107349
1115	23	-0.36646326353892533
1115	24	-0.4366096306736509
1115	25	-0.6500219378458283
1116	1	-1.5134121158191078
1116	2	-0.7767295456159431
1116	3	-1.7606677600837912
1116	4	-1.0586129641643396
1116	5	-0.9920693297955339
1116	6	-0.469210885039125
1116	7	-1.274757703609751
1116	8	-3.0205164041026435
1116	9	-1.5645627063765772
1116	10	-0.8028145791513026
1116	11	-0.6530743937712771
1116	12	-0.7552365134079722
1116	13	-1.653962335051233
1116	14	-1.6034820002204242
1116	15	-1.4464550132897003
1116	16	-0.9595351677009489
1116	17	-1.699481156610183
1116	18	-2.509369634831586
1116	19	-1.3455385139410012
1116	20	-0.9473643111492862
1116	21	-0.7697628466215178
1116	22	-0.5127245446733042
1116	23	-0.4927816203493336
1116	24	-0.5689939438092466
1116	25	-0.33623319324942713
1201	1	0.3851970080191407
1201	2	0.3612193982384362
1201	3	0.47913984201252546
1201	4	0.46041987894076897
1201	5	0.37296723135465015
1201	6	0.2532687438961716
1201	7	0.3512361336492802
1201	8	0.37451529231567093
1201	9	0.33797684619139545
1201	10	0.48135814461095094
1201	11	0.3455784173767995
1201	12	0.4763421257113123
1201	13	0.38963084670199066
1201	14	0.8420824908238131
1201	15	0.7793886225705506
1201	16	0.6617003957308398
1201	17	0.7469431253514713
1201	18	0.609319706641763
1201	19	0.5490167133759272
1201	20	0.6345670770759355
1201	21	0.6230524824400447
1201	22	0.7130070846587913
1201	23	0.6456599896975703
1201	24	0.6719221782372315
1201	25	0.7529258806804862
1202	1	0.4500181956034872
1202	2	0.5010953003683447
1202	3	0.488692297566125
1202	4	0.5634997357734903
1202	5	0.3325566522312542
1202	6	0.1684886288929448
1202	7	0.30333772961243
1202	8	0.341060208116879
1202	9	0.35442714836823797
1202	10	0.5470612191870217
1202	11	0.36955659023369236
1202	12	0.40146387657676996
1202	13	0.2992167470716396
1202	14	0.7844324292149452
1202	15	0.5992325467622948
1202	16	0.3965529745021783
1202	17	0.5073126318937469
1202	18	0.592595831737114
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2210	23	0.6998229158751244
2210	24	0.6294810822494625
2210	25	0.8100192644054829
2211	1	-0.005822306376632724
2211	2	-0.025425589243237567
2211	3	-0.011036452204390201
2211	4	-0.06657501048264436
2211	5	-0.004105665164949812
2211	6	-0.11848526237092472
2211	7	-0.01552068524240724
2211	8	4.146365047219547E-4
2211	9	-0.05961039523374012
2211	10	0.15862767778808262
2211	11	-0.22173166804740418
2211	12	0.01310331112789953
2211	13	-0.19412080439836643
2211	14	-0.1545880014284009
2211	15	-0.11825414315875804
2211	16	-0.1325266630440467
2211	17	-0.06394369531126642
2211	18	-0.11810140658542423
2211	19	-0.21111172591260186
2211	20	-0.14171236803471743
2211	21	-0.09824541832515621
2211	22	-0.08006657697792008
2211	23	-0.03891872647128736
2211	24	0.04218704087114844
2211	25	-0.04091660402041697
2212	1	0.234798518748363
2212	2	0.19955488838379765
2212	3	0.2007107261357568
2212	4	0.18427865585553604
2212	5	0.3075601441589271
2212	6	0.25415502081935415
2212	7	0.224320722980913
2212	8	0.3236952607789263
2212	9	0.1157543640120764
2212	10	0.3974049695802577
2212	11	0.19682848241806994
2212	12	0.27435393433513866
2212	13	0.21378069653621168
2212	14	0.21042231206234163
2212	15	0.2952202904107679
2212	16	0.09927444450935496
2212	17	0.17984050439748955
2212	18	0.2953645214148466
2212	19	0.2248257487275538
2212	20	0.28302187754555064
2212	21	0.21734260179528775
2212	22	-0.27213680408889473
2212	23	0.25412395771012086
2212	24	0.25260656956891125
2212	25	0.3042274047486302
2213	1	0.0398484834510171
2213	2	-0.6576103681649101
2213	3	-0.06115616495474896
2213	4	0.020200295749909003
2213	5	0.19257752854523602
2213	6	0.13254182665303915
2213	7	0.12999039686854275
2213	8	0.3098955554005016
2213	9	0.0019281307830141259
2213	10	-1.4057786963253065
2213	11	-0.2513398632489654
2213	12	0.04144078982954369
2213	13	0.09497590808942391
2213	14	0.21246710845652217
2213	15	0.2240065913639313
2213	16	0.13973477035639092
2213	17	0.056403161415310876
2213	18	0.16446684067708617
2213	19	0.2118505011603986
2213	20	0.1682746384999021
2213	21	0.07568946804080667
2213	22	0.26579392223415255
2213	23	-0.6083643139400863
2213	24	-1.2076486357709655
2213	25	-1.1786942010914885
2214	1	0.07446240390544645
2214	2	-0.10568580769315616
2214	3	0.06220053151720961
2214	4	-0.055707473184270384
2214	5	0.10326534281443145
2214	6	-0.03366148890842169
2214	7	0.03097374768340444
2214	8	0.10362305575235098
2214	9	-0.31502252494549765
2214	10	0.05280148539381457
2214	11	-0.015296097337653691
2214	12	0.06585871760564999
2214	13	0.03786087764898838
2214	14	0.12055275662986986
2214	15	-0.06502020396030161
2214	16	-0.14096062730492065
2214	17	-0.08240628433369324
2214	18	0.19378781152871483
2214	19	-0.6249278214279457
2214	20	-0.23380171079343626
2214	21	-0.1716352741420053
2214	22	-0.4185199592245823
2214	23	-1.6950575476831702
2214	24	-1.2060006442911444
2214	25	-1.454497847088021
2215	1	-0.3207914595044663
2215	2	-0.1088018862846134
2215	3	-1.1937086277539954
2215	4	-0.801870392275859
2215	5	-0.1456560311970989
2215	6	-0.16116624539437652
2215	7	-0.15517030852197422
2215	8	0.023813269150622318
2215	9	-0.22142924007928144
2215	10	-1.227409058409016
2215	11	-0.48132947388445046
2215	12	-0.2910704724278119
2215	13	-0.35406790569964386
2215	14	-0.648840306554753
2215	15	-0.8843570410496469
2215	16	-0.5409974975045486
2215	17	-0.43159824762770427
2215	18	-0.29953661054721437
2215	19	-0.8600754806306696
2215	20	-1.1500305838045648
2215	21	-0.5669020382851429
2215	22	-0.24859620111918446
2215	23	-0.3657996151230307
2215	24	-1.6787684913285403
2215	25	-0.6194616329554137
2216	1	-0.5375805501050692
2216	2	-0.47795559644437446
2216	3	-1.3818131297654155
2216	4	-0.7847781345370848
2216	5	-0.5074188454629791
2216	6	-0.5690325592281127
2216	7	-0.3039153211370831
2216	8	-0.5150381750142046
2216	9	-0.28445291213687796
2216	10	-0.301366922490125
2216	11	-0.4310041584887898
2216	12	-0.2537464750100753
2216	13	-1.0010249051485829
2216	14	-0.5827739113586077
2216	15	-1.0653170073807132
2216	16	-0.7697415687068556
2216	17	-1.6248669402648375
2216	18	-0.7937165854082693
2216	19	-0.6232731127090076
2216	20	-0.5469465977911696
2216	21	-0.23181706862065
2216	22	-0.4058559435336093
2216	23	-0.3166957425695571
2216	24	-0.4556545519222368
2216	25	-0.2690686602261394
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4769.0
3	511.0
4	635.0
5	507.0
6	362.0
7	315.0
8	579.0
9	1358.0
10	2464.0
11	4247.0
12	6722.0
13	9740.0
14	12980.0
15	16785.0
16	19346.0
17	20575.0
18	21404.0
19	21456.0
20	21993.0
21	23666.0
22	25357.0
23	26509.0
24	27924.0
25	29463.0
26	30782.0
27	34851.0
28	38833.0
29	45606.0
30	55243.0
31	70000.0
32	93317.0
33	131153.0
34	190622.0
35	306280.0
36	554684.0
37	1822492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03457624082537	21.089423421666957	12.76789329951703	23.10810703799064
2	14.275105832276806	22.068746596227264	40.359656569727946	23.296491001767986
3	18.492775918455298	27.67506498536636	29.736403533894677	24.095755562283667
4	11.845222570580809	17.91706543600175	38.25558377102474	31.982128222392703
5	12.187823882977243	39.487212233641635	35.14544068278557	13.17952320059555
6	30.524416281812517	38.42058148706988	17.768506002197295	13.286496228920303
7	24.809486444566083	33.864297564704785	23.798368869590025	17.527847121139104
8	24.111910538787097	37.75197930642026	21.486802714259134	16.649307440533512
9	24.37323564811935	17.21622783613938	22.080001047562618	36.330535468178645
10	14.787620907268664	30.893657260547474	33.66935775401201	20.649364078171853
11	31.4072882003164	26.791364355463422	22.465386503736838	19.335960940483336
12	20.42100267047826	29.11156816266186	31.809596809610447	18.657832357249436
13	26.297160899976348	24.528237544955577	26.394526570679595	22.78007498438848
14	19.881711973948484	24.34746412065357	28.360511701278273	27.410312204119673
15	24.02685321612895	30.104475845143142	23.793893683608168	22.074777255119738
16	20.8462896408457	31.867934039029304	26.85613425244015	20.429642067684842
17	20.351496312815517	31.11194879074096	28.80542641833042	19.731128478113106
18	20.479808318892424	30.235872615203828	29.658117956219833	19.626201109683915
19	20.60459740831492	29.62075050141717	30.054534657862053	19.720117432405857
20	22.120950190835163	28.928172714758094	28.67753534739692	20.27334174700982
21	21.587464027212082	29.845187794931853	28.5066223205711	20.06072585728496
22	20.59758381778175	29.561532018055768	28.615674498669875	21.225209665492606
23	19.872336920112513	30.17629845261502	29.03649044095344	20.91487418631903
24	20.525435880759044	29.980695423101984	28.608804589447633	20.885064106691345
25	21.370940461926928	28.660376987894224	27.26437711010866	22.704305440070186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9940.0
1	9940.0
2	8768.0
3	7596.0
4	7596.0
5	7596.0
6	9849.5
7	12103.0
8	12103.0
9	12103.0
10	16225.5
11	20348.0
12	20348.0
13	20348.0
14	30414.5
15	40481.0
16	40481.0
17	40481.0
18	60696.0
19	80911.0
20	80911.0
21	80911.0
22	112122.5
23	143334.0
24	143334.0
25	143334.0
26	182902.0
27	222470.0
28	222470.0
29	222470.0
30	263381.0
31	304292.0
32	304292.0
33	304292.0
34	340769.0
35	377246.0
36	377246.0
37	377246.0
38	401798.5
39	426351.0
40	426351.0
41	426351.0
42	422784.5
43	419218.0
44	419218.0
45	419218.0
46	463773.0
47	508328.0
48	508328.0
49	508328.0
50	454515.5
51	400703.0
52	400703.0
53	400703.0
54	337118.0
55	273533.0
56	273533.0
57	273533.0
58	230406.0
59	187279.0
60	187279.0
61	187279.0
62	154319.0
63	121359.0
64	121359.0
65	121359.0
66	93297.5
67	65236.0
68	65236.0
69	65236.0
70	48051.0
71	30866.0
72	30866.0
73	30866.0
74	22208.5
75	13551.0
76	13551.0
77	13551.0
78	9450.5
79	5350.0
80	5350.0
81	5350.0
82	3655.5
83	1961.0
84	1961.0
85	1961.0
86	1353.0
87	745.0
88	745.0
89	745.0
90	493.0
91	241.0
92	241.0
93	241.0
94	153.0
95	65.0
96	65.0
97	65.0
98	44.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3100832169602535
2	0.18083423845728766
3	0.19926337882091613
4	0.2201152569871486
5	0.24532261884345577
6	0.1961600966917379
7	0.190361859029326
8	0.21374536209041442
9	0.21445312819005155
10	0.22379019635064912
11	0.1995355965515458
12	0.20470773343350948
13	0.21687586599265554
14	0.20587826967521702
15	0.19675897569912318
16	0.20040669328956073
17	0.1992089352747902
18	0.20255721336153507
19	0.20201277790027578
20	0.19809284257920856
21	0.19645953619543055
22	0.19961726187073467
23	0.203455531872613
24	0.21989748280264487
25	0.21347314435978473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3673530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.09889848977886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3672987651675	57.60588514741376
2	11.6564622855492	16.108974084381998
3	2.8001392879378657	5.8045962124338075
4	0.9728535301866472	2.688924293111606
5	0.3973513407544394	1.3728269979784269
6	0.2253059360600881	0.9341035202976381
7	0.12818404099207717	0.6200163225564834
8	0.08789495578226268	0.4858755701889736
9	0.06052920313473194	0.3764251136766641
>10	0.2678846800875105	3.2523200060533997
>50	0.018653692943030258	0.8647377293934477
>100	0.013992241654578103	1.9185181686694914
>500	0.0013086357794879979	0.6095760651828843
>1k	0.001625880792470181	2.801183658296392
>5k	3.172450326771083E-4	1.626942769793596
>10k+	1.9827814542319272E-4	2.9290943405714023
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	34482	0.9386611787572172	No Hit
CCCATGTACTCTGCGTTGATACCAC	31703	0.8630118714152328	No Hit
GAGTACATGGGAAGCAGTGGTATCA	17407	0.4738494037070611	No Hit
CATGTACTCTGCGTTGATACCACTG	12700	0.3457165178996769	No Hit
GCGTTGATACCACTGCTTCCCATGT	10603	0.28863245978663576	No Hit
GTATCAACGCAGAGTACATGGGAAG	9071	0.24692870345417078	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8509	0.2316300669927835	No Hit
GCTTCCCATGTACTCTGCGTTGATA	7981	0.21725697081553708	No Hit
TATCAACGCAGAGTACATGGGAAGC	7722	0.2102065315922287	No Hit
GGTATCAACGCAGAGTACATGGGAA	7359	0.20032502797037183	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7170	0.19518011286147113	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5990	0.16305842064717044	No Hit
ATTCCATTCCATTCCATTCCATTCC	5572	0.15167971950685036	No Hit
GTGGTATCAACGCAGAGTACATGGG	4745	0.12916731318377692	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4687	0.12758845034612484	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4283	0.1165908540286863	No Hit
GAATGGAATGGAATGGAATGGAATG	4037	0.1098942978551965	No Hit
CAGTGGTATCAACGCAGAGTACATG	3893	0.1059743625341293	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3874	0.10545714884593292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.7221773062966685E-5	2.7221773062966685E-5	0.0	2.7221773062966685E-5	0.0
6	2.7221773062966685E-5	2.7221773062966685E-5	0.0	5.444354612593337E-5	0.0
7	2.7221773062966685E-5	2.7221773062966685E-5	0.0	5.444354612593337E-5	0.0
8	2.7221773062966685E-5	2.7221773062966685E-5	0.0	8.166531918890005E-5	0.0
9	5.444354612593337E-5	2.7221773062966685E-5	0.0	8.166531918890005E-5	0.0
10	5.444354612593337E-5	2.7221773062966685E-5	0.0	1.0888709225186674E-4	0.0
11	8.166531918890005E-5	2.7221773062966685E-5	0.0	1.3610886531483342E-4	2.7221773062966685E-5
12	8.166531918890005E-5	2.7221773062966685E-5	0.0	1.3610886531483342E-4	2.7221773062966685E-5
13	8.166531918890005E-5	2.7221773062966685E-5	0.0	1.633306383778001E-4	2.7221773062966685E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACCG	70	1.0855628E-4	12.221642	5
CAGAACG	85	6.5610826E-4	10.064881	4
GTGCGTT	85	6.6189567E-4	10.055271	11
GTTCTAG	345	0.0	9.92484	1
CTAGGAC	340	0.0	9.783965	3
CCGGGAC	80	0.004500606	9.504423	3
GACTCCG	120	1.7029106E-5	9.499495	7
GCTACAC	305	0.0	9.348613	3
TATTCCG	245	1.8189894E-12	9.311726	5
TAGGACT	420	0.0	9.279394	4
GTCTTAG	350	0.0	9.2395525	1
TATACTG	785	0.0	9.202991	5
GTCCTAA	275	0.0	8.992507	1
TCTAGAC	255	3.6379788E-12	8.945339	3
GTACCGT	85	0.007442854	8.940579	6
GGGTCGG	85	0.0074595287	8.938141	17
TCAGGAC	395	0.0	8.902878	3
CTAGACT	330	0.0	8.641564	4
TAGGGTG	300	0.0	8.555149	5
ACGCAAA	180	3.9655606E-7	8.4544935	1
>>END_MODULE