FastQCFastQC Report
Wed 25 May 2016
SRR1295065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673530
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC355140.9667540485581988No Hit
GTACATGGGAAGCAGTGGTATCAAC334900.9116571798787542No Hit
GAGTACATGGGAAGCAGTGGTATCA178640.4862897539968368No Hit
GTATCAACGCAGAGTACTTTTTTTT171450.4667172991645638No Hit
CATGTACTCTGCGTTGATACCACTG144620.39368128203662417No Hit
GGTATCAACGCAGAGTACTTTTTTT144430.3931640683484278No Hit
TATCAACGCAGAGTACTTTTTTTTT114780.3124515112167316No Hit
GCGTTGATACCACTGCTTCCCATGT112000.30488385830522685No Hit
ACGCAGAGTACATGGGAAGCAGTGG107790.29342349184571787No Hit
GTATCAACGCAGAGTACATGGGAAG95320.2594779408361984No Hit
GCTTCCCATGTACTCTGCGTTGATA89940.24483262692832236No Hit
TATCAACGCAGAGTACATGGGAAGC84150.22907122032486466No Hit
GGTATCAACGCAGAGTACATGGGAA83080.2261584906071272No Hit
ACTCTGCGTTGATACCACTGCTTCC81720.2224563294705637No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68400.1861969277506921No Hit
ATTCCATTCCATTCCATTCCATTCC54830.1492569817042463No Hit
GTGGTATCAACGCAGAGTACATGGG50690.1379871676561781No Hit
CAGTGGTATCAACGCAGAGTACATG48910.13314169205097007No Hit
GAATGGAATGGAATGGAATGGAATG45150.12290630537929456No Hit
ACATGGGAAGCAGTGGTATCAACGC44200.12032023693831274No Hit
CATGGGAAGCAGTGGTATCAACGCA40660.11068372927402254No Hit
GCAGTGGTATCAACGCAGAGTACAT39720.10812488260610366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA307.7287486E-418.9988027
AACGCCG400.005284080314.2487146
ACGATTC1158.8373235E-710.73816118
GTTCCCG750.002618278610.1476121
AGCGGTT750.00265042810.13269412
CGCACTG750.002651026710.1324185
GTCTTAG4150.010.0864821
TAGGACT4750.09.9990974
ATAGGAC2950.09.982153
CTAGGAC3600.09.7630073
TCCACGA900.00111491769.4994012
TATACTG8650.09.2246015
AACGATT1356.8611516E-69.14732317
TAGACAG5200.09.1337915
GAACGAT1151.1124782E-49.08613716
CTAGAAC4500.09.0769583
TAGGACA4100.09.0357694
TCTGTCG1901.0604708E-88.9994338
GTCCTAG3550.08.843431
TAGCACC2701.8189894E-128.7955034
Produced by FastQC (version 0.11.5)