FastQCFastQC Report
Wed 25 May 2016
SRR1295064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217310
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC250870.5948578596309022No Hit
CCCATGTACTCTGCGTTGATACCAC217020.5145934256670721No Hit
GAGTACATGGGAAGCAGTGGTATCA127680.30275222831615417No Hit
CATGTACTCTGCGTTGATACCACTG91620.21724748714227793No Hit
GCGTTGATACCACTGCTTCCCATGT76570.18156123215983647No Hit
GTATCAACGCAGAGTACATGGGAAG74140.1757992654085187No Hit
GCTTCCCATGTACTCTGCGTTGATA71210.16885170879067463No Hit
GGTATCAACGCAGAGTACATGGGAA67770.16069485050897372No Hit
GTATCAACGCAGAGTACTTTTTTTT67100.1591061600878285No Hit
ACGCAGAGTACATGGGAAGCAGTGG62400.14796161534248134No Hit
TATCAACGCAGAGTACATGGGAAGC62260.14762965017985397No Hit
GGTATCAACGCAGAGTACTTTTTTT54830.13001178476327327No Hit
ACTCTGCGTTGATACCACTGCTTCC53200.12614676179839754No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN50270.11919920518055349No Hit
ATTCCATTCCATTCCATTCCATTCC49680.11780020913805245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3150.09.9511738
TATTCCG1304.209358E-69.5059645
GACAGCG900.00111558339.4988477
GTCTAAT3550.09.3763161
TAGGACT4300.09.2853374
CTGTCGC3400.09.219369
GTCTTAG4500.09.08758451
CGCTCTG2950.09.0226115
GTCGTAG850.00737356358.9508361
ATAGGAC3250.08.7742163
TCGCTCT3150.08.751944
TCGCCCA3150.08.74330812
CCTACAC4400.08.6412733
GTTCTAG4750.08.6092911
GTCCTAA3100.08.5899151
ACAGCGT1000.00290972938.5489638
TCTCGCT2951.8189894E-128.3776412
GTAGGAC2053.8295184E-88.3462053
GTATAGA7300.08.3377661
TAGTACT4450.08.3314674
Produced by FastQC (version 0.11.5)