##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295064_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4217310 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23881905764575 33.0 33.0 33.0 27.0 33.0 2 31.21212526468294 33.0 33.0 33.0 27.0 33.0 3 31.264736763481935 33.0 33.0 33.0 27.0 33.0 4 34.80818104431498 37.0 37.0 37.0 33.0 37.0 5 35.16283981969549 37.0 37.0 37.0 33.0 37.0 6 35.21235076387555 37.0 37.0 37.0 33.0 37.0 7 35.039805231296725 37.0 37.0 37.0 33.0 37.0 8 34.953076250026676 37.0 37.0 37.0 33.0 37.0 9 34.90576267810524 37.0 37.0 37.0 33.0 37.0 10 34.80280842527583 37.0 37.0 37.0 33.0 37.0 11 34.997277174312536 37.0 37.0 37.0 33.0 37.0 12 34.90058117615257 37.0 37.0 37.0 33.0 37.0 13 34.83767116953698 37.0 37.0 37.0 33.0 37.0 14 36.27401945790089 40.0 37.0 40.0 27.0 40.0 15 36.43160711448767 40.0 37.0 40.0 33.0 40.0 16 36.52910148886375 40.0 37.0 40.0 33.0 40.0 17 36.45520580654493 40.0 37.0 40.0 33.0 40.0 18 36.387170257818376 40.0 37.0 40.0 27.0 40.0 19 36.49087854580289 40.0 37.0 40.0 33.0 40.0 20 36.49121549044296 40.0 37.0 40.0 33.0 40.0 21 36.501250323073236 40.0 37.0 40.0 33.0 40.0 22 36.41148054091352 40.0 37.0 40.0 27.0 40.0 23 36.317213105036146 40.0 37.0 40.0 27.0 40.0 24 36.365671482532704 40.0 37.0 40.0 27.0 40.0 25 35.33313439135373 37.0 33.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37418889475636163 1101 2 0.3097913450207628 1101 3 0.355991134131731 1101 4 0.3278839973125969 1101 5 0.29529814321116277 1101 6 0.24227185669111861 1101 7 0.2593977750652243 1101 8 0.44619684915296176 1101 9 0.3587877064704159 1101 10 0.4887933466985288 1101 11 0.4593755044541439 1101 12 0.48478456410521176 1101 13 0.41724616968714656 1101 14 0.8498648966968148 1101 15 0.7401823625048394 1101 16 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25 -0.8471116193187385 2216 1 -0.4565101565902019 2216 2 -0.39657390322386377 2216 3 -1.3559520984145834 2216 4 -0.7127046744740042 2216 5 -0.528141039256333 2216 6 -0.4466833085979829 2216 7 -0.3042280232838621 2216 8 -0.3973076223994525 2216 9 -0.22755836555626274 2216 10 -0.41074223481152927 2216 11 -0.46952288540594367 2216 12 -0.3897634483837038 2216 13 -0.9575347111184556 2216 14 -0.6739571344426096 2216 15 -1.03231256956591 2216 16 -0.8592990125800029 2216 17 -1.7284391012764715 2216 18 -0.7975724279793397 2216 19 -0.4918653791292087 2216 20 -0.6337746286463641 2216 21 -0.43239605838876827 2216 22 -0.4691739601799654 2216 23 -0.3302905168924468 2216 24 -0.540434161733927 2216 25 -0.44051986289743894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5589.0 3 528.0 4 687.0 5 662.0 6 415.0 7 313.0 8 483.0 9 884.0 10 1812.0 11 3335.0 12 5601.0 13 8458.0 14 11569.0 15 14722.0 16 17137.0 17 18355.0 18 18930.0 19 19372.0 20 20177.0 21 21859.0 22 23596.0 23 25555.0 24 27517.0 25 29354.0 26 31178.0 27 35784.0 28 40356.0 29 47883.0 30 58343.0 31 74386.0 32 100443.0 33 143658.0 34 209793.0 35 337254.0 36 625877.0 37 2235445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76003592686751 21.749170332932454 13.916570887058716 22.57422285314132 2 14.484035168903286 21.623953470472927 41.16615529923309 22.725856061390697 3 17.991353050203582 27.396009820182694 30.39048602681519 24.222151102798534 4 11.80048222364702 18.824687987612183 39.45479165191305 29.920038136827753 5 12.21610877462629 39.3222907783026 35.730176135273716 12.731424311797396 6 29.346498446306647 39.67177025057953 18.167889165201355 12.81384213791247 7 24.816212630772696 33.63857653166436 24.198757392119948 17.346453445442993 8 23.266539961722373 37.811279021301736 21.906053939425913 17.016127077549985 9 23.61281743336709 17.680859729775587 22.72773718777075 35.97858564908657 10 14.291397688213225 30.96155395496063 34.455875492336624 20.29117286448952 11 31.033897785599372 26.608660446928727 23.66829109742094 18.689150670050957 12 20.144205237627375 28.672178416261048 32.33243477320762 18.851181572903958 13 25.91803547881235 24.579066649968667 27.079988089605518 22.42290978161346 14 19.578580495174332 24.559906551871457 28.929642550453387 26.931870402500817 15 22.868714890009684 30.865880906152558 24.526029980165916 21.73937422367184 16 20.494015479563533 32.14030210690759 27.06991939459819 20.29576301893069 17 19.67279408532672 31.207043739680817 29.256339178010197 19.863822996982268 18 19.747268006257592 30.630478006238583 30.141865035992556 19.48038895151127 19 20.294970784244402 29.89180951403383 30.355279364970745 19.457940336751026 20 21.51830763981749 29.32460789708528 29.349816719270184 19.807267743827044 21 21.335319264394535 29.921041896286976 28.86139292520375 19.882245914114737 22 20.291742777472596 29.676894184912666 29.457115322839705 20.574247714775034 23 19.86816131052685 30.202526809211932 29.453256500693335 20.476055379567878 24 20.232972580795032 29.816017868978413 29.339421473050027 20.611588077176535 25 20.936235212260968 29.1597912274465 28.24954802700241 21.654425533290116 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8747.0 1 8747.0 2 7243.0 3 5739.0 4 5739.0 5 5739.0 6 8430.0 7 11121.0 8 11121.0 9 11121.0 10 16760.0 11 22399.0 12 22399.0 13 22399.0 14 36163.0 15 49927.0 16 49927.0 17 49927.0 18 76992.0 19 104057.0 20 104057.0 21 104057.0 22 145723.0 23 187389.0 24 187389.0 25 187389.0 26 237280.5 27 287172.0 28 287172.0 29 287172.0 30 337449.0 31 387726.0 32 387726.0 33 387726.0 34 424922.0 35 462118.0 36 462118.0 37 462118.0 38 482918.5 39 503719.0 40 503719.0 41 503719.0 42 493918.0 43 484117.0 44 484117.0 45 484117.0 46 508896.5 47 533676.0 48 533676.0 49 533676.0 50 479300.5 51 424925.0 52 424925.0 53 424925.0 54 363639.5 55 302354.0 56 302354.0 57 302354.0 58 255167.5 59 207981.0 60 207981.0 61 207981.0 62 167789.0 63 127597.0 64 127597.0 65 127597.0 66 96162.5 67 64728.0 68 64728.0 69 64728.0 70 45734.0 71 26740.0 72 26740.0 73 26740.0 74 18404.5 75 10069.0 76 10069.0 77 10069.0 78 6674.5 79 3280.0 80 3280.0 81 3280.0 82 2219.0 83 1158.0 84 1158.0 85 1158.0 86 766.0 87 374.0 88 374.0 89 374.0 90 258.5 91 143.0 92 143.0 93 143.0 94 91.0 95 39.0 96 39.0 97 39.0 98 27.0 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3133276899255687 2 0.18215402709309964 3 0.1998193161043414 4 0.22061456236321256 5 0.24596247370954472 6 0.1969739004246783 7 0.19201813478259838 8 0.21596705008642952 9 0.2158722028971074 10 0.2268981886558019 11 0.200388399240274 12 0.20688543170883808 13 0.21864648318477894 14 0.2079998861833728 15 0.19903682679243404 16 0.2023801902160382 17 0.2020956486480719 18 0.205391588477015 19 0.20444311658379394 20 0.2007440762002319 21 0.19844403185917092 22 0.20235647841870766 23 0.2058658244236255 24 0.2216341696484252 25 0.21563508492380215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4217310.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.77512607752779 #Duplication Level Percentage of deduplicated Percentage of total 1 86.27817797208516 65.37739813574133 2 10.652341614220441 16.143650576768994 3 1.887610118608206 4.2910168416826195 4 0.515924715947101 1.5637704158961714 5 0.206760078972123 0.7833635525956111 6 0.1035137114977955 0.47062587236989745 7 0.06595440354307597 0.34983922706913084 8 0.042720349937002106 0.2589711922041953 9 0.03652090195497288 0.2490638355092791 >10 0.18099058362399645 2.5611444781377597 >50 0.01570340838513538 0.8124471295960306 >100 0.011199538267441144 1.624204563223957 >500 0.0010393403703605859 0.5434412545561431 >1k 0.0011023304194351657 1.804255158147841 >5k 3.4644670325098136E-4 1.7454505826833757 >10k+ 9.448546452299493E-5 1.421357183817682 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 25087 0.5948578596309022 No Hit CCCATGTACTCTGCGTTGATACCAC 21702 0.5145934256670721 No Hit GAGTACATGGGAAGCAGTGGTATCA 12768 0.30275222831615417 No Hit CATGTACTCTGCGTTGATACCACTG 9162 0.21724748714227793 No Hit GCGTTGATACCACTGCTTCCCATGT 7657 0.18156123215983647 No Hit GTATCAACGCAGAGTACATGGGAAG 7414 0.1757992654085187 No Hit GCTTCCCATGTACTCTGCGTTGATA 7121 0.16885170879067463 No Hit GGTATCAACGCAGAGTACATGGGAA 6777 0.16069485050897372 No Hit GTATCAACGCAGAGTACTTTTTTTT 6710 0.1591061600878285 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6240 0.14796161534248134 No Hit TATCAACGCAGAGTACATGGGAAGC 6226 0.14762965017985397 No Hit GGTATCAACGCAGAGTACTTTTTTT 5483 0.13001178476327327 No Hit ACTCTGCGTTGATACCACTGCTTCC 5320 0.12614676179839754 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 5027 0.11919920518055349 No Hit ATTCCATTCCATTCCATTCCATTCC 4968 0.11780020913805245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.742359466105171E-5 0.0 4 0.0 0.0 0.0 7.113539199157757E-5 0.0 5 0.0 0.0 0.0 7.113539199157757E-5 0.0 6 0.0 0.0 0.0 7.113539199157757E-5 0.0 7 0.0 0.0 0.0 7.113539199157757E-5 0.0 8 0.0 0.0 0.0 7.113539199157757E-5 0.0 9 0.0 0.0 0.0 1.1855898665262928E-4 0.0 10 0.0 0.0 0.0 1.1855898665262928E-4 0.0 11 0.0 0.0 0.0 1.4227078398315515E-4 0.0 12 0.0 0.0 0.0 1.65982581313681E-4 0.0 13 0.0 0.0 0.0 1.8969437864420685E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGTCG 315 0.0 9.951173 8 TATTCCG 130 4.209358E-6 9.505964 5 GACAGCG 90 0.0011155833 9.498847 7 GTCTAAT 355 0.0 9.376316 1 TAGGACT 430 0.0 9.285337 4 CTGTCGC 340 0.0 9.21936 9 GTCTTAG 450 0.0 9.0875845 1 CGCTCTG 295 0.0 9.022611 5 GTCGTAG 85 0.0073735635 8.950836 1 ATAGGAC 325 0.0 8.774216 3 TCGCTCT 315 0.0 8.75194 4 TCGCCCA 315 0.0 8.743308 12 CCTACAC 440 0.0 8.641273 3 GTTCTAG 475 0.0 8.609291 1 GTCCTAA 310 0.0 8.589915 1 ACAGCGT 100 0.0029097293 8.548963 8 TCTCGCT 295 1.8189894E-12 8.377641 2 GTAGGAC 205 3.8295184E-8 8.346205 3 GTATAGA 730 0.0 8.337766 1 TAGTACT 445 0.0 8.331467 4 >>END_MODULE