FastQCFastQC Report
Wed 25 May 2016
SRR1295064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217310
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC253670.6014971628834495No Hit
GTACATGGGAAGCAGTGGTATCAAC233910.5546426513583302No Hit
GTATCAACGCAGAGTACTTTTTTTT155840.36952464959891496No Hit
GGTATCAACGCAGAGTACTTTTTTT141780.3361858625521956No Hit
GAGTACATGGGAAGCAGTGGTATCA132270.3136359432908655No Hit
TATCAACGCAGAGTACTTTTTTTTT105060.24911614275450467No Hit
CATGTACTCTGCGTTGATACCACTG100500.23830356317178486No Hit
GCGTTGATACCACTGCTTCCCATGT84400.20012756946963822No Hit
GCTTCCCATGTACTCTGCGTTGATA82200.19491097405692254No Hit
GTATCAACGCAGAGTACATGGGAAG75870.17990140634669965No Hit
ACGCAGAGTACATGGGAAGCAGTGG75320.17859725749352076No Hit
GGTATCAACGCAGAGTACATGGGAA70810.1679032368974536No Hit
TATCAACGCAGAGTACATGGGAAGC64720.15346275232316334No Hit
ACTCTGCGTTGATACCACTGCTTCC59520.1411326177112899No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59200.14037384019671306No Hit
ATTCCATTCCATTCCATTCCATTCC48280.11448055751177882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCA600.00588143811.0825878
CTGCGCA750.002650662610.132659
TATTCCG2400.09.8951675
TATACTG10250.09.6383745
GGAACGA1901.0550139E-99.4993615
TATACCG1002.7592195E-49.4993595
TAGACAG6250.09.2713755
TTAGACA6350.09.1253694
GTCTACG850.0073661798.9519311
ACGCGAG850.0074432788.94057517
GACCGTG850.0074432788.9405757
GTATTAG6550.08.8579691
TAGACTG4850.08.8138395
GTCTAGG4250.08.7281311
TGGACTG5550.08.5579825
CAGATCG1000.0029083128.5494239
GTATAGG5350.08.5336151
GCCCTAG2701.4551915E-118.45461
GTCTTAC3750.08.3700541
ATACTGT11600.08.1891046
Produced by FastQC (version 0.11.5)