FastQCFastQC Report
Wed 25 May 2016
SRR1295063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750517
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC114490.4162490179119053No Hit
CCCATGTACTCTGCGTTGATACCAC106560.38741807449290444No Hit
GAGTACATGGGAAGCAGTGGTATCA61960.22526674076182768No Hit
ATTCCATTCCATTCCATTCCATTCC48230.17534885259752986No Hit
CATGTACTCTGCGTTGATACCACTG46270.16822291954567087No Hit
GTATCAACGCAGAGTACTTTTTTTT38440.13975554413951993No Hit
GAATGGAATGGAATGGAATGGAATG36880.13408388313906075No Hit
GGTATCAACGCAGAGTACTTTTTTT34880.1268125228820618No Hit
GCGTTGATACCACTGCTTCCCATGT34230.12444933079853715No Hit
GCTTCCCATGTACTCTGCGTTGATA33390.12139535949059758No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32340.11757789535567313No Hit
ACGCAGAGTACATGGGAAGCAGTGG31530.11463299445158856No Hit
GTATCAACGCAGAGTACATGGGAAG29690.10794334301514952No Hit
GGTATCAACGCAGAGTACATGGGAA29170.10605278934832979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG1102.728484E-912.9612725
AGACGGT658.0311863E-411.6915566
GTCCTAC1701.0913936E-1111.1875781
CGATCAA600.005877224311.08342910
CCGAGGG803.7723582E-410.6875929
GGAACGA1451.4520992E-810.47941115
GTACTAG1356.360515E-79.8616431
TTAGACT2350.09.7071664
GTTCTAG3150.09.6603851
GTCTAAT3100.09.5094411
CTTAGAC2002.6375346E-109.5030263
GAACGAA1106.862386E-59.49713916
GACTGTG3800.09.249917
TAGACTG3700.09.2480425
GTATTAG4450.09.1888991
ACATTCG1151.114763E-49.0842219
GTCTAGG3350.09.0836461
CTAGGAC2201.6734703E-109.0710713
CGTGTGA1054.4938512E-49.04769610
GTATAAA6550.09.0013041
Produced by FastQC (version 0.11.5)