##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295063_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2750517
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09152642939491	33.0	33.0	33.0	27.0	33.0
2	31.015255677387195	33.0	33.0	33.0	27.0	33.0
3	31.09105451811423	33.0	33.0	33.0	27.0	33.0
4	34.65848347783344	37.0	37.0	37.0	27.0	37.0
5	34.89646819125277	37.0	37.0	37.0	33.0	37.0
6	34.7983222790479	37.0	37.0	37.0	33.0	37.0
7	34.72453978652013	37.0	37.0	37.0	27.0	37.0
8	34.63152818179273	37.0	37.0	37.0	27.0	37.0
9	34.60350290509021	37.0	37.0	37.0	27.0	37.0
10	34.51275923762696	37.0	37.0	37.0	27.0	37.0
11	34.67851571177346	37.0	37.0	37.0	27.0	37.0
12	34.581178738397185	37.0	37.0	37.0	27.0	37.0
13	34.50680144860039	37.0	37.0	37.0	27.0	37.0
14	35.86602191515268	40.0	37.0	40.0	27.0	40.0
15	36.02228344707559	40.0	37.0	40.0	27.0	40.0
16	36.1161272589844	40.0	37.0	40.0	27.0	40.0
17	36.02909416666031	40.0	37.0	40.0	27.0	40.0
18	35.97169841160771	40.0	37.0	40.0	27.0	40.0
19	36.0854628420766	40.0	37.0	40.0	27.0	40.0
20	36.08938683163929	40.0	37.0	40.0	27.0	40.0
21	36.08400202580097	40.0	37.0	40.0	27.0	40.0
22	35.98078615765691	40.0	37.0	40.0	27.0	40.0
23	35.88630464745355	37.0	37.0	40.0	27.0	40.0
24	35.93819125640743	40.0	37.0	40.0	27.0	40.0
25	34.79620522250908	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.37784646338073813
1101	2	0.36097701000745275
1101	3	0.3476986425018893
1101	4	0.39433409997649704
1101	5	0.2421380262847137
1101	6	0.22368510740609082
1101	7	0.21608198788527488
1101	8	0.48764737898203236
1101	9	0.38820155850701354
1101	10	0.5607318984642973
1101	11	0.5193972952023884
1101	12	0.39297734899834325
1101	13	0.3941520453150389
1101	14	0.878686684502604
1101	15	0.8380094151085871
1101	16	0.7329553164557012
1101	17	0.723242491369227
1101	18	0.7494873209700543
1101	19	0.6318298932672448
1101	20	0.6166718233695292
1101	21	0.6528983582608063
1101	22	0.6968744368794262
1101	23	0.8196588260112705
1101	24	0.8240999673969753
1101	25	0.6954177082007931
1102	1	0.3525651747882108
1102	2	0.32272297242788994
1102	3	0.3760162604253807
1102	4	0.332857480646922
1102	5	0.24483066740814508
1102	6	0.22213015191180574
1102	7	0.26831245985303553
1102	8	0.383049670570216
1102	9	0.3604311301196006
1102	10	0.552962254596892
1102	11	0.29094586133413713
1102	12	0.4952049119238211
1102	13	0.35944506485090244
1102	14	0.7412146922614511
1102	15	0.7128628112245821
1102	16	0.6815843940839557
1102	17	0.572016784175787
1102	18	0.6946871511135768
1102	19	0.5714671343789348
1102	20	0.7452292464161445
1102	21	0.5326189375103567
1102	22	0.5611439210785818
1102	23	0.6953218836226824
1102	24	0.619470035747625
1102	25	0.7102609318829778
1103	1	0.2907055542643313
1103	2	0.15976589340582947
1103	3	0.21696592447974083
1103	4	0.2590377985239911
1103	5	0.20571184399329212
1103	6	0.060576903715961805
1103	7	0.11125381452724525
1103	8	0.17457403068662813
1103	9	0.2109641710858554
1103	10	0.4495133569241645
1103	11	0.22960197056786313
1103	12	0.3510710674289683
1103	13	0.41880898612818385
1103	14	0.6320458827316315
1103	15	0.576342292032578
1103	16	0.5146899167201653
1103	17	0.2854739046726138
1103	18	0.3994517006434677
1103	19	0.3697636032530198
1103	20	0.46319449416478164
1103	21	0.445182006210608
1103	22	0.46196692478967805
1103	23	0.6622872287468695
1103	24	0.49923557706551236
1103	25	0.6206528864987604
1104	1	0.29164629709148215
1104	2	0.3054057505061323
1104	3	0.3154394590587515
1104	4	0.26722053870470575
1104	5	0.11658342677156952
1104	6	0.039448184564932376
1104	7	0.15183063121967422
1104	8	0.3161234042339771
1104	9	0.28470280567641737
1104	10	0.40492179230217573
1104	11	0.22902141522057207
1104	12	0.34692384548255717
1104	13	0.24920678305741717
1104	14	0.5283508961766046
1104	15	0.4066724588231949
1104	16	0.4752830760962894
1104	17	0.41200888085262477
1104	18	0.4265118615778647
1104	19	0.3092022072453773
1104	20	0.4738714439898075
1104	21	0.3681971249660947
1104	22	0.5220229944806363
1104	23	0.45216798693391524
1104	24	0.49420916331698805
1104	25	0.6292373547688257
1105	1	0.18478514568595017
1105	2	-0.0165026725317432
1105	3	0.10925142233193696
1105	4	0.09060066895346353
1105	5	0.09613160477413629
1105	6	0.03469380987900905
1105	7	0.05893251496421925
1105	8	0.02766802796317336
1105	9	0.07412533289295453
1105	10	0.27242414723716024
1105	11	0.01987012768657337
1105	12	0.1728125155495519
1105	13	0.2264712336098853
1105	14	0.4950868036963243
1105	15	0.2749596225455946
1105	16	0.2583762704867567
1105	17	0.13643037047361872
1105	18	0.1636426250650871
1105	19	0.21524309868236458
1105	20	0.25190127384448147
1105	21	0.2272529943999544
1105	22	0.267307021002388
1105	23	0.33340322408553646
1105	24	0.30911025604751075
1105	25	0.2458857278800437
1106	1	0.18342633496730798
1106	2	0.010439700311092537
1106	3	0.018390019672381186
1106	4	0.17986326385984341
1106	5	-0.29307053090184354
1106	6	-0.23973109688877514
1106	7	-0.5931775108239634
1106	8	0.04478986826151754
1106	9	0.2332556777987307
1106	10	-0.18450396106941724
1106	11	-0.1729294742484484
1106	12	-0.4359846068670663
1106	13	-0.08681817602717246
1106	14	-0.09536617108991408
1106	15	-0.03595342412070579
1106	16	0.09787527735359447
1106	17	-0.11344246086095922
1106	18	-0.23626717988253176
1106	19	-0.4742417978740434
1106	20	0.03206256970941723
1106	21	0.09211741501545845
1106	22	0.3685929397242944
1106	23	-0.3529045527653878
1106	24	0.20504089933967862
1106	25	0.3783795811690709
1107	1	-1.1405710524982382
1107	2	-1.1598836794834817
1107	3	-1.0175828241078868
1107	4	-1.1291529643967948
1107	5	-1.0583423763244255
1107	6	-1.0573414150662899
1107	7	-1.1435885952827718
1107	8	-1.0528922874924334
1107	9	-1.1711712720647043
1107	10	-0.9346092399414161
1107	11	-0.8905395270034404
1107	12	-0.9786340246951326
1107	13	-0.8771773049984972
1107	14	-1.2284588633022722
1107	15	-0.9928322339453999
1107	16	-0.9514105774580841
1107	17	-1.072384639471025
1107	18	-1.3224514262272606
1107	19	-1.233591225263794
1107	20	-1.4526638855164862
1107	21	-1.1964449653299667
1107	22	-1.1841588476859926
1107	23	-1.1152194618267757
1107	24	-1.0213454138178548
1107	25	-0.8157950548087953
1108	1	-0.13686030710434594
1108	2	-0.3605757367949529
1108	3	-0.2679995794998504
1108	4	-0.3044278438148922
1108	5	-0.6670355963597814
1108	6	-0.3489187649346377
1108	7	-0.27524442901325585
1108	8	-0.06984422711190774
1108	9	-0.400206583042646
1108	10	-0.698911926712384
1108	11	-0.259466238218657
1108	12	-0.1218581160635992
1108	13	-0.08807192638653305
1108	14	-0.5477463907226152
1108	15	-0.14675034303188284
1108	16	-1.0052957314344937
1108	17	-0.9837350553814161
1108	18	-0.8537160236510175
1108	19	-0.2941696465957264
1108	20	-1.2607027353662446
1108	21	-0.45725593143859555
1108	22	-0.5352492393278965
1108	23	-0.40545207420370133
1108	24	-0.6173098493390725
1108	25	-1.3546656916367183
1109	1	0.11210826158936982
1109	2	0.06956725709068934
1109	3	0.15885898287765343
1109	4	0.18983677301228852
1109	5	0.08663431957596401
1109	6	0.17331691451422415
1109	7	0.2331860893721398
1109	8	0.33212845037697036
1109	9	0.227516389094383
1109	10	0.3313127872672865
1109	11	0.24841568112914558
1109	12	0.23780569202767055
1109	13	0.21492306622284474
1109	14	0.5899181075941513
1109	15	0.3891071970304978
1109	16	0.36078880165400307
1109	17	0.4759525199085104
1109	18	0.3856532014080756
1109	19	0.3844616883394565
1109	20	0.3858891134613458
1109	21	0.3730381306686752
1109	22	0.44673620082108556
1109	23	0.5119432361698699
1109	24	0.5203689744124986
1109	25	0.4078697149777213
1110	1	0.03838633955573556
1110	2	0.012819182299828924
1110	3	0.15141495627390356
1110	4	-0.9065355661644148
1110	5	-0.2785686248004495
1110	6	-0.03768471666955264
1110	7	0.03658438366311856
1110	8	0.2290475807354042
1110	9	0.17042432744866431
1110	10	-0.0013970895615003087
1110	11	-0.03372246311054994
1110	12	0.16626177642498163
1110	13	0.20148869579904272
1110	14	0.3341490892596184
1110	15	0.22212423869947884
1110	16	0.194844570326147
1110	17	0.299221956031289
1110	18	0.3703907910515838
1110	19	0.23140122816498376
1110	20	0.17868770302082737
1110	21	0.2148334462160335
1110	22	-0.27069716628149365
1110	23	0.18326993335789155
1110	24	0.2135618113032507
1110	25	0.3535534667444722
1111	1	-0.1610604216951934
1111	2	-0.2818167175316191
1111	3	-0.22691277746347893
1111	4	-0.09523695897657092
1111	5	-0.2478104401741561
1111	6	-0.06607064228642656
1111	7	-0.036896422332674206
1111	8	0.08720978203206187
1111	9	0.03721050539670756
1111	10	0.07193931805980469
1111	11	-0.0876050159939652
1111	12	-0.03399547892790622
1111	13	-0.1117652783170513
1111	14	-0.1563914863508984
1111	15	-0.08222494717981732
1111	16	-0.19592205195631607
1111	17	-0.06113915968238359
1111	18	-0.1714451444469276
1111	19	-0.15164241008847767
1111	20	-0.0930959895450556
1111	21	-0.07988163982077623
1111	22	-0.0799588913296887
1111	23	0.014346459838357362
1111	24	0.008685804257673624
1111	25	0.14914858434929812
1112	1	-0.07542344333452888
1112	2	-0.39574329603793146
1112	3	-0.11350776942662222
1112	4	-0.17747046130938315
1112	5	-0.11026463975150591
1112	6	-0.026194939721804644
1112	7	-0.0022948035586836113
1112	8	0.15913126149077783
1112	9	0.032124245606901525
1112	10	0.1724909171188429
1112	11	-0.07591757150780865
1112	12	0.12245316953031704
1112	13	-0.036508103235512124
1112	14	-0.15703239279073955
1112	15	-0.15911360318132495
1112	16	-0.08702027091877085
1112	17	-0.10889371515993673
1112	18	-0.07156518298825176
1112	19	-0.006657141585833415
1112	20	-0.8589185820934873
1112	21	-1.1883140630349516
1112	22	-1.6789220988015643
1112	23	-0.532641358238628
1112	24	-0.26979759679625204
1112	25	-1.468996932649155
1113	1	-0.22406111747908497
1113	2	-0.15131041185652094
1113	3	-0.2495247338713824
1113	4	-0.5083844863401552
1113	5	-0.26710474510236537
1113	6	-0.1737316387321144
1113	7	-0.11499646404861608
1113	8	-0.10547645285222274
1113	9	-0.2701875368100346
1113	10	-0.10404591756534387
1113	11	-0.2341311094442844
1113	12	-0.18751545371569733
1113	13	-0.34740154752421404
1113	14	-0.6442865273217109
1113	15	-0.6199188112052667
1113	16	-0.4622366881134141
1113	17	-0.4702486243057251
1113	18	-0.8453235499457392
1113	19	-1.0936061450004502
1113	20	-1.1913071196391556
1113	21	-1.074405426167516
1113	22	-0.4917049416728858
1113	23	-1.9687255999403774
1113	24	-1.022342543349815
1113	25	-0.6442357562968581
1114	1	-0.24969804381544236
1114	2	-0.36859102651987996
1114	3	-0.28250985142221197
1114	4	-0.5163969177664285
1114	5	-0.5283933964038923
1114	6	-0.34502504467122463
1114	7	-0.22392065865058441
1114	8	-0.105287676398234
1114	9	-1.0770896375216452
1114	10	-0.22077461852320823
1114	11	-0.26109525167298386
1114	12	-0.32285064843905786
1114	13	-0.23014210472727115
1114	14	-0.531239238187716
1114	15	-1.2327186135048365
1114	16	-0.9636471824222994
1114	17	-1.4611886190452736
1114	18	-1.6156143993639063
1114	19	-1.5188949278132284
1114	20	-0.8193277235503871
1114	21	-0.551448041593936
1114	22	-0.317290311262866
1114	23	-0.30968333424718253
1114	24	-0.9333368840304388
1114	25	-0.5613341700688323
1115	1	-0.6126890673907184
1115	2	-0.44860533861748664
1115	3	-0.5605377519049135
1115	4	-0.5465758147193256
1115	5	-0.3256276710186512
1115	6	-0.24837477532727803
1115	7	-0.2064872948211942
1115	8	-0.14324772029890198
1115	9	-0.5504828762716727
1115	10	-0.2531933971559397
1115	11	-0.41346711700783345
1115	12	-0.4424253649280985
1115	13	-0.41346296369209057
1115	14	-0.4333578894880077
1115	15	-1.5286967177877102
1115	16	-0.8944923557377962
1115	17	-0.6275818595021718
1115	18	-2.185576191098562
1115	19	-1.0411277942609587
1115	20	-0.8780232402879449
1115	21	-0.7787635802476913
1115	22	-0.6035891874602299
1115	23	-0.33706484339258935
1115	24	-0.39130079398967865
1115	25	-0.40029610347362876
1116	1	-1.401830920637778
1116	2	-0.7119339054222529
1116	3	-1.6460369024305024
1116	4	-0.8897014575122455
1116	5	-0.954221494539496
1116	6	-0.40511276165985777
1116	7	-1.247966310189156
1116	8	-2.829225426035542
1116	9	-1.3773997012128945
1116	10	-0.7729567974800844
1116	11	-0.5086387020588461
1116	12	-0.6061272854147361
1116	13	-1.5077676737752697
1116	14	-1.5590470572824557
1116	15	-1.3539488761950338
1116	16	-0.8929009231970753
1116	17	-1.5785676050142428
1116	18	-2.331192508031343
1116	19	-1.2362438651514651
1116	20	-0.8377412315453654
1116	21	-0.6824123492181897
1116	22	-0.5288598239813638
1116	23	-0.3689895564530232
1116	24	-0.41719685280356344
1116	25	-0.4024794342993161
1201	1	0.18333957131332568
1201	2	0.19617501210824173
1201	3	0.26602113344134537
1201	4	0.2405297854761983
1201	5	0.16376977670021375
1201	6	-0.03587136979939487
1201	7	-0.044035124303036355
1201	8	0.17580810602367336
1201	9	0.14003497028146228
1201	10	0.291351965533444
1201	11	0.21687977336828368
1201	12	0.18418621021649528
1201	13	0.221903317693652
1201	14	0.6179977883403254
1201	15	0.41607146561510433
1201	16	0.33498610519107785
1201	17	0.42755452296228214
1201	18	0.29521610264811926
1201	19	0.41078043873163494
1201	20	0.3346079221304734
1201	21	0.42486963527421295
1201	22	0.3332620363343963
1201	23	0.4048586199142079
1201	24	0.4409489598865406
1201	25	0.455379132691462
1202	1	0.14249190350830787
1202	2	0.19291325451929353
1202	3	0.23975669303321823
1202	4	0.34621333485207373
1202	5	0.2224163259520111
1202	6	0.022906099943462266
1202	7	0.05647723517439118
1202	8	0.07673288877769124
1202	9	0.09579882795441108
1202	10	0.22957905393901257
1202	11	0.05857215292787288
1202	12	0.12018331111747926
1202	13	0.0803432146115668
1202	14	0.4354424861282382
1202	15	0.2333259863044077
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2210	18	0.36366410515437764
2210	19	0.33583111782799335
2210	20	0.29729695886426555
2210	21	0.46700719594624474
2210	22	0.4843400740284949
2210	23	0.5675672720866132
2210	24	0.5477977114485384
2210	25	0.5688502115617595
2211	1	0.007775412362736489
2211	2	-0.11955548752406742
2211	3	0.05061777331056305
2211	4	-0.05867032027157393
2211	5	-0.05303224381739824
2211	6	-0.06494800182706939
2211	7	-0.02918696354380046
2211	8	0.05117500108008244
2211	9	-0.06757502872656573
2211	10	0.1352720929774165
2211	11	0.037519132698889734
2211	12	0.020744167324089346
2211	13	-0.05338725894980456
2211	14	-0.09216895996984675
2211	15	-0.04949630072943734
2211	16	-0.20875655072267563
2211	17	-0.10688419033613883
2211	18	-0.003800688109599548
2211	19	-0.18600516354882046
2211	20	0.019624584237831755
2211	21	-0.02786725988351435
2211	22	-0.14524145360130092
2211	23	0.02111225057596755
2211	24	0.06734429209311799
2211	25	-0.09646472629171399
2212	1	0.1255109610706029
2212	2	0.1821355765594035
2212	3	0.14733590097358018
2212	4	0.090546913056059
2212	5	0.24457715797770874
2212	6	0.14984947609810462
2212	7	0.26975869206633263
2212	8	0.360974248052635
2212	9	0.21164030309589776
2212	10	0.34576587011041227
2212	11	0.17614405992069493
2212	12	0.2846677868637073
2212	13	0.20075430369497838
2212	14	0.15240620668658522
2212	15	0.20517577989595281
2212	16	0.19436450804482064
2212	17	0.08658866258685549
2212	18	0.20195885135330371
2212	19	0.16962084864945837
2212	20	0.1905025910051492
2212	21	0.2031934836893896
2212	22	-0.3553448236610919
2212	23	0.24346276607225548
2212	24	0.19595148566968135
2212	25	0.369229883343408
2213	1	-0.12766167244324933
2213	2	-0.919299943873991
2213	3	-0.2846573469413407
2213	4	-0.10157336790015137
2213	5	-0.041491458180523466
2213	6	-0.02107585801262246
2213	7	-0.07923345725741626
2213	8	0.15331096350949736
2213	9	-0.30100553570272126
2213	10	-1.5315782136787774
2213	11	-0.4806776403014723
2213	12	-0.11952236609622702
2213	13	-0.16280485075103002
2213	14	0.07232927246742094
2213	15	0.05443938449361241
2213	16	-0.10076602582086736
2213	17	-0.14145720970584819
2213	18	0.046335987376110666
2213	19	0.07060425375668444
2213	20	-0.007395783291542557
2213	21	0.005247677691613717
2213	22	0.07467161689621804
2213	23	-0.9090254097519548
2213	24	-1.3480222619783433
2213	25	-1.3724870822098723
2214	1	0.18541562626215224
2214	2	-0.041242197862537466
2214	3	0.09027811115693396
2214	4	0.14440145366661739
2214	5	0.14698194550921073
2214	6	0.08148720597309023
2214	7	0.22858747240004362
2214	8	0.19268461252371338
2214	9	-0.05924057768736901
2214	10	0.2675518563301509
2214	11	0.18514658088421498
2214	12	0.10542454291444159
2214	13	0.1553426961694342
2214	14	0.21565954236264417
2214	15	0.19592101944027007
2214	16	0.0366723754925431
2214	17	0.10809267198256123
2214	18	0.23586616409779992
2214	19	-0.25150982482685436
2214	20	-0.1486177279035843
2214	21	0.012309827779660054
2214	22	-0.3028777120855324
2214	23	-1.4001567825197014
2214	24	-0.9021283451102278
2214	25	-1.4441285931246952
2215	1	-0.376362733096844
2215	2	-0.17932771870033548
2215	3	-1.1731028630785865
2215	4	-0.9369900230268442
2215	5	-0.27456206619693546
2215	6	-0.32297678109295447
2215	7	-0.1984542598251835
2215	8	-0.08214971976097019
2215	9	-0.3134183501585923
2215	10	-1.4278122463428602
2215	11	-0.5023198732786653
2215	12	-0.3977268571011763
2215	13	-0.5464903588782732
2215	14	-0.7080031895350132
2215	15	-0.9811044453304731
2215	16	-0.5077186370691393
2215	17	-0.4874216098546995
2215	18	-0.5027736890296239
2215	19	-1.0343110529136865
2215	20	-1.3648474081448967
2215	21	-0.6551440972535687
2215	22	-0.3232959295108344
2215	23	-0.49212286603979294
2215	24	-1.702509330746345
2215	25	-0.7551689903691425
2216	1	-0.5050955990927193
2216	2	-0.3992223427219592
2216	3	-1.3449380543479101
2216	4	-0.7172886891748931
2216	5	-0.5389983417346258
2216	6	-0.35260513398419846
2216	7	-0.2407810049324013
2216	8	-0.3110226779247256
2216	9	-0.20041146176240687
2216	10	-0.19386543082624996
2216	11	-0.284973276852412
2216	12	-0.2662693793255144
2216	13	-1.0032845333053544
2216	14	-0.6267691264688793
2216	15	-0.9827089111974985
2216	16	-0.6789814851239413
2216	17	-1.646766753666732
2216	18	-0.695558339432111
2216	19	-0.5074809717556263
2216	20	-0.38276448132387486
2216	21	-0.18464325719214258
2216	22	-0.30471126979908547
2216	23	-0.3749879478883713
2216	24	-0.40934889936784913
2216	25	-0.15647014467207754
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3590.0
3	355.0
4	462.0
5	411.0
6	259.0
7	223.0
8	449.0
9	1000.0
10	1829.0
11	3108.0
12	5039.0
13	7283.0
14	9550.0
15	12116.0
16	13827.0
17	14911.0
18	15306.0
19	15670.0
20	16289.0
21	17728.0
22	18813.0
23	20116.0
24	20978.0
25	22255.0
26	23155.0
27	26133.0
28	29206.0
29	34095.0
30	41284.0
31	52732.0
32	69055.0
33	96531.0
34	140186.0
35	223051.0
36	402107.0
37	1391415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59627076387121	21.82598065138725	12.6555216050756	22.92222697966594
2	14.628598527134804	21.89104993456644	40.82398572517613	22.656365813122626
3	17.91166080699212	27.294972410167745	30.82545820032036	23.967908582519772
4	11.533919595701228	17.702354340764543	39.49182092945722	31.271905134077
5	12.11941190620239	39.14100255851417	35.90623154917668	12.833353986106758
6	29.938309719087925	39.574123959761984	17.598140731067165	12.88942559008293
7	24.338158317469823	33.85743020601801	24.69556994624255	17.10884153026962
8	23.243699952853937	38.30402432066317	21.979391269307477	16.472884457175418
9	23.439291563359063	16.959960067549964	22.932266039017936	36.66848233007303
10	14.13207730739561	30.88415077412704	34.741103159729725	20.242668758747623
11	31.620459047734233	26.597133629184494	23.246695717492496	18.535711605588777
12	19.351036897544546	28.863469357940687	32.965957234420124	18.819536510094643
13	25.7175108388598	24.934460377996466	27.356265551427335	21.991763231716405
14	19.192067128334266	24.175687255176275	29.202989472579745	27.42925614390972
15	22.61637793485721	31.299679284020883	24.34825879346095	21.73568398766096
16	20.72681145481782	32.63394652630843	26.6099958615303	20.029246157343454
17	19.16398545149332	31.52030729057378	29.684949931221	19.630757326711898
18	19.30946496540549	31.154588217464546	30.329689786021135	19.20625703110883
19	19.896500847009964	29.77711798029108	30.49884333047961	19.827537842219346
20	21.4274421230943	29.466639951913443	29.351450793246027	19.754467131746235
21	21.45010857563556	30.300962700325783	28.656172054041196	19.59275666999746
22	19.897406043578933	29.943795910813716	30.118695983527843	20.040102062079505
23	19.692767399506938	30.568244786404996	29.759542364341605	19.979445449746454
24	20.07335427469376	29.40015427279905	29.783868691038663	20.742622761468528
25	20.892483104446203	29.227513674750615	28.50483685210939	21.375166368693794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6921.0
1	6921.0
2	5783.5
3	4646.0
4	4646.0
5	4646.0
6	6357.5
7	8069.0
8	8069.0
9	8069.0
10	12063.5
11	16058.0
12	16058.0
13	16058.0
14	25625.5
15	35193.0
16	35193.0
17	35193.0
18	52317.5
19	69442.0
20	69442.0
21	69442.0
22	95861.0
23	122280.0
24	122280.0
25	122280.0
26	154337.0
27	186394.0
28	186394.0
29	186394.0
30	218708.0
31	251022.0
32	251022.0
33	251022.0
34	277491.5
35	303961.0
36	303961.0
37	303961.0
38	321936.0
39	339911.0
40	339911.0
41	339911.0
42	331858.5
43	323806.0
44	323806.0
45	323806.0
46	325809.0
47	327812.0
48	327812.0
49	327812.0
50	296762.5
51	265713.0
52	265713.0
53	265713.0
54	230431.5
55	195150.0
56	195150.0
57	195150.0
58	165585.0
59	136020.0
60	136020.0
61	136020.0
62	110264.0
63	84508.0
64	84508.0
65	84508.0
66	63935.5
67	43363.0
68	43363.0
69	43363.0
70	31279.5
71	19196.0
72	19196.0
73	19196.0
74	13269.5
75	7343.0
76	7343.0
77	7343.0
78	4872.0
79	2401.0
80	2401.0
81	2401.0
82	1645.0
83	889.0
84	889.0
85	889.0
86	595.0
87	301.0
88	301.0
89	301.0
90	193.5
91	86.0
92	86.0
93	86.0
94	51.0
95	16.0
96	16.0
97	16.0
98	16.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3132138430702301
2	0.181202297604414
3	0.19938069824691143
4	0.21857708932538863
5	0.24493577025700985
6	0.1945816004772921
7	0.18989157311152777
8	0.21286907152364445
9	0.21385070515833934
10	0.22541216796696764
11	0.1978900693942266
12	0.20527050005508057
13	0.21792266690225875
14	0.20668841530519536
15	0.19665393815053678
16	0.2014530359201561
17	0.19970790945847633
18	0.20261645356127594
19	0.20239831275356596
20	0.19876263262506652
21	0.1972720037723817
22	0.20072589989445622
23	0.20454336402938064
24	0.21915879814594857
25	0.21305085553006944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2750517.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.88262707367298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41930996603122	70.53630081235612
2	8.237519258797194	12.995943194077961
3	1.2914859624050674	3.0562741662984774
4	0.3784300408471186	1.1940622314247236
5	0.17369411956801975	0.6850724229387033
6	0.10533796896524737	0.49856014335502485
7	0.06787248873608914	0.37477721522716934
8	0.04716409454547801	0.2976342145038717
9	0.03849048540847995	0.2732607545725598
>10	0.20609179808726116	3.0436073083439803
>50	0.019387479839204674	1.0753039567282248
>100	0.012479826956416374	1.7927651404389529
>500	0.0013450641455866434	0.7775827296253022
>1k	0.0012523011008218974	2.363408063746392
>5k	4.6381522252662864E-5	0.2266928241709777
>10k+	9.276304450532573E-5	0.8087548221916497
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11449	0.4162490179119053	No Hit
CCCATGTACTCTGCGTTGATACCAC	10656	0.38741807449290444	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6196	0.22526674076182768	No Hit
ATTCCATTCCATTCCATTCCATTCC	4823	0.17534885259752986	No Hit
CATGTACTCTGCGTTGATACCACTG	4627	0.16822291954567087	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3844	0.13975554413951993	No Hit
GAATGGAATGGAATGGAATGGAATG	3688	0.13408388313906075	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3488	0.1268125228820618	No Hit
GCGTTGATACCACTGCTTCCCATGT	3423	0.12444933079853715	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3339	0.12139535949059758	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3234	0.11757789535567313	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3153	0.11463299445158856	No Hit
GTATCAACGCAGAGTACATGGGAAG	2969	0.10794334301514952	No Hit
GGTATCAACGCAGAGTACATGGGAA	2917	0.10605278934832979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.6356801284994786E-5	0.0
10	0.0	0.0	0.0	3.6356801284994786E-5	0.0
11	0.0	0.0	0.0	1.0907040385498435E-4	0.0
12	0.0	0.0	0.0	1.0907040385498435E-4	0.0
13	0.0	0.0	0.0	1.0907040385498435E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	110	2.728484E-9	12.961272	5
AGACGGT	65	8.0311863E-4	11.691556	6
GTCCTAC	170	1.0913936E-11	11.187578	1
CGATCAA	60	0.0058772243	11.083429	10
CCGAGGG	80	3.7723582E-4	10.687592	9
GGAACGA	145	1.4520992E-8	10.479411	15
GTACTAG	135	6.360515E-7	9.861643	1
TTAGACT	235	0.0	9.707166	4
GTTCTAG	315	0.0	9.660385	1
GTCTAAT	310	0.0	9.509441	1
CTTAGAC	200	2.6375346E-10	9.503026	3
GAACGAA	110	6.862386E-5	9.497139	16
GACTGTG	380	0.0	9.24991	7
TAGACTG	370	0.0	9.248042	5
GTATTAG	445	0.0	9.188899	1
ACATTCG	115	1.114763E-4	9.08422	19
GTCTAGG	335	0.0	9.083646	1
CTAGGAC	220	1.6734703E-10	9.071071	3
CGTGTGA	105	4.4938512E-4	9.047696	10
GTATAAA	655	0.0	9.001304	1
>>END_MODULE