FastQCFastQC Report
Wed 25 May 2016
SRR1295063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750517
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC117640.4277014103166787No Hit
CCCATGTACTCTGCGTTGATACCAC116980.42530186143186893No Hit
GTATCAACGCAGAGTACTTTTTTTT107270.389999407384139No Hit
GGTATCAACGCAGAGTACTTTTTTT104670.38054663905004044No Hit
TATCAACGCAGAGTACTTTTTTTTT75470.27438477929785565No Hit
GAGTACATGGGAAGCAGTGGTATCA67130.24406320702617No Hit
CATGTACTCTGCGTTGATACCACTG52000.1890553666819729No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48360.17582149101423478No Hit
ATTCCATTCCATTCCATTCCATTCC46570.16931362358422072No Hit
ACGCAGAGTACATGGGAAGCAGTGG39260.14273680184488952No Hit
GAATGGAATGGAATGGAATGGAATG38740.1408462481780698No Hit
GCGTTGATACCACTGCTTCCCATGT37340.13575629599817055No Hit
GCTTCCCATGTACTCTGCGTTGATA36670.13332039031207588No Hit
GGTATCAACGCAGAGTACATGGGAA33920.12332226995870231No Hit
GTATCAACGCAGAGTACATGGGAAG33220.12077729386875267No Hit
TATCAACGCAGAGTACATGGGAAGC28610.10401680847637008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT402.7638805E-416.6235858
CGATCAA400.005283452614.24878810
TCTGTCG1603.6379788E-1211.873998
GGGACGC700.001493095310.8562187
CTAGGAC3200.010.389933
TGTCGCC1752.3646862E-1010.31340810
GACAGTC2151.8189894E-1210.1619267
TGTACCG750.002650151310.1326555
CTGTCGC1901.0004442E-109.9991499
AACACGG1151.0278774E-59.9123795
TATACTG9150.09.8627275
GTCGCCC1752.579327E-99.77059711
GCTAGAC1502.6527414E-79.4993643
TCCGGAT1401.0605017E-69.49919118
CTGCTCG800.00452048759.4991919
GTGCGAT900.00111495549.49919116
GACTGTC1951.6862032E-99.2556227
TGGACCC1356.8568224E-69.1475355
TAGACTG3950.09.1386285
GTACTAG2106.493792E-109.0606841
Produced by FastQC (version 0.11.5)